list of human diseases caused by bacteria and viruses
Tài liệu Báo cáo khoa học: Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate specificity by replacement of Asn149 with Asp docx
... His120 and His145, and thecorresponding His101 and His126 in arginase I, weredescribed among the ligands for coordination of Mn2+A and Mn2+B, respectively [9,24]. However, thevolume of the ... the interaction of human arginase IIwith substrate and metal ions was examined by site-directed mutagenesis and kinetic studies. Selection of target residues (Asp149, His120 and His145) was ... proteinband was detected by SDS ⁄ PAGE and Coomassie bluestaining of purified enzymes.Metal-free species of purified enzymes were obtained by incubation for 1 h at 25 °C with 25 mm EDTA and 3 mV....
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Báo cáo khoa học: Inhibition of recombinant human maltase glucoamylase by salacinol and derivatives pdf
... providing lead candi-dates for the treatment of Type 2 diabetes [23]. Inorder to examine the mechanism of action of thisclass of inhibitors, 3, 5, the stereoisomers of salacinol6, 7 [25], and the ... four-carbon chain of salacinol and the seven-carbon chain of kotalanol.Fig. 2. Schematic diagram of MGA proteinorganization and expression construct.Amino acid boundaries of each of thedomains ... method of action of acarbose is quitecomplex and it appears to be acting as a type of sui-cide inhibitor of a-amylase in a mechanism wherebythe acarbose is rearranged into an active entity by...
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Báo cáo khoa học: Transcription factor specificity protein 1 (SP1) and activating protein 2a (AP-2a) regulate expression of human KCTD10 gene by binding to proximal region of promoter pot
... structure of human and mouse KCTD10 genes, and the 5Â-ankingregion sequence of human KCTD10.(A) Genomic organization of human and mouse KCTD10 genes. Solid boxes indicatecoding region, and open ... Alignment of the 5Â-ankingsequences upstream of the start codon of human and mouse KCTD10.Fig. 5. In vivo ChIP assays of Sp1.1 and AP-2.2 binding sites in theproximal promoter region of human ... transcription, respectively.Roles of Sp1 and AP-2a in the regulation of thepromoter activity and the expression of human KCTD10To test the effects of Sp1 and AP-2a binding onKCTD10 promoter...
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Tài liệu Báo cáo khoa học: Biochemical characterization of human 3-methylglutaconyl-CoA hydratase and its role in leucine metabolism docx
... 3, 4, and 5 were isolated by HPLC and used as substrates for AUH. The AUH assay contained 2 mM of the respective isomers of 3-MG-CoA in a total volume of 25 lL. The reaction was started by addition ... CoA-esters were separated by HPLC, ionized by ESI and detected by TOF. The HPLC system consisted of aHP1100 series binary-gradient pump, a vacuum degasser (allfrom Hewlett-Packard), and a CTC HTS PAL ... proceduresProduction and purification of human AUHin E. coliThe production of AUHp40 (precursor form), AUHp32(mature form), AUHp40A240 V and AUHp32A240 V inE. coli was performed using the pMAL-c2 bacterial...
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Tài liệu Báo cáo khoa học: Multidentate pyridinones inhibit the metabolism of nontransferrin-bound iron by hepatocytes and hepatoma cells docx
... inMaterials and methods. Results are the mean and standard deviation of iron internalized by normal and iron-loaded hepatocytes from threeseparate experiments, and are expressed as a percentage of the ... determine the effect of chelators on uptake, intracellular distribution and mobiliza-tion of NTBI; (c) to assess and compare the activity and toxicity of novel tetradentate and hexadentate pyridinoneswith ... overloadconditions, and is also implicated in intracellular Femetabolism [3,9,10]. An important aspect of this workwas the assessment of the effect of novel Fe chelators on theuptake and fate of NTBI and...
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Tài liệu Báo cáo khoa học: Oxygen-dependent regulation of hypoxia-inducible factors by prolyl and asparaginyl hydroxylation pdf
... Fellow supported by theCancer Council of South Australia, and this work was also supported by the National Heart Foundation and National Health and MedicalResearch Council of Australia.References1. ... CAD of HIF-1a and HIF-2a.Other mechanisms of activationThe induction of HIF activity by well established agentssuch as hypoxia, cobaltous ions and iron chelators can beeasily explained by ... neuronal cultures by iron chelators is associated withenhanced DNA binding of hypoxia-inducible factor-1 and ATF-1/CREB and increased expression of glycolytic enzymes, p21(waf1/cip1), and erythropoietin....
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Tài liệu Báo cáo Y học: Regulation of transcription of the Dnmt1 gene by Sp1 and Sp3 zinc finger proteins doc
... isoforms of Sp3 exist and that thesedifferent isoforms play different roles in the activation and repression of transcription [9]. In our hands, Sp1 generateda single band and Sp3 generated two bands ... aresimilar and binding of these factors is independentlyregulated by different coactivators in a cell-cycle-dependentmanner. The distinct functions of Sp1 and Sp3 in theregulation of expression of ... progression of the cellcycle and the differentiation of cells [11,12].The methylation of DNA plays a role in the regulation of gene expression [13,14], genomic imprinting [15] and inactivation of the...
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Báo cáo khoa học: Fluorescence quenching and kinetic studies of conformational changes induced by DNA and cAMP binding to cAMP receptor protein from Escherichia coli ppt
... The plots of the inverse time constant(kobs) are linear (Fig. 9) and the values of koff and theassociation-rate parameter, kon, listed in Table 3, weredetermined as the intercept and the ... , A and k arethe amplitude and the observed rate constant, respectively, and F1is the fluorescence at infinite time. The validity of the fitting was evaluated by an inspection of residuals and normalized ... nuclear magnetic resonance study of the cyclicAMP receptor protein (CRP): assignments of the NHprotons of histidine and tryptophan residues and theeffect of binding of cAMP to CRP. Biochem J 107,304–309.14...
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Báo cáo Y học: High affinity binding between laminin and laminin binding protein of Leishmania is stimulated by zinc and may involve laminin zinc-finger like sequences doc
... cofactor forL. donovani interaction with ECM and BM. Both Zn2+ and free sulfhydryls may be required for LBP binding site onlaminin a s e videnced by the stimulatory and inhibitoryeffects of ... NaCl/Pi and immediately f rozenin liquid nitrogen. Cells were lysed in 100 lLofSDS/PAGEsample buffe r by boiling for 5 min, p roteins were resolved by means of 7.5% S DS/PAGE a nd analysed by immuno-blotting ... for 30 min and harvested by centrifugation for 10 min at 2000 g. Cells were solubi-lized by boiling in SDS sample buffer for 5 min and theextracted proteins were resolved by means of 7.5% SDS/PAGE...
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