lipids proteins and nucleotides
Tài liệu Báo cáo khoa học: Analysis of proteins and peptides on a chromatographic timescale by electron-transfer dissociation MS ppt
... peptides and proteins N D Udeshi et al dimethylation of Arg, monomethylation, dimethylation and trimethylation of Lys, acetylation and ubiquitination of Lys, and phosphorylation of Ser and Thr ... Hart [11], this modification is found on proteins in both the nucleus and cytoplasm, is added and removed by transferase and O-GlcNAcase enzymes, respectively, and often occurs at sites that are known ... reactions and tandem MS ETD has most recently been utilized for the direct analysis of intact proteins, and ubiquitin is a model protein that was initially interrogated via ETD for tandem MS Shown...
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Tài liệu Báo cáo khoa học: Inhibition of pneumococcal choline-binding proteins and cell growth by esters of bicyclic amines pptx
... analysis of ligand binding to C-LytA (A) Conformational changes induced by ligands on C-LytA monitored by near-UV CD with no ligand added (—) and upon addition of ligands: s, d, 2.5 and 20 mM choline; ... 10 Experimental conditions depended on the enzyme, and were set as follows: 37 °C and pH 6.9 for LytA; 30 °C and pH 6.0 for LytC; and 30 °C and pH 6.9 for Pce The specific activities of the enzymes ... single-step purification system for CBPs and CBM-containing fusion proteins ([26], and C-LYTAG Purification System User’s Manual from Biomedal, http://www.biomedal.es), and gave support to the hypothesis...
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Tài liệu Báo cáo khoa học: The small heat shock proteins and their role in human disease pptx
... 2005 FEBS Small heat shock proteins and disease up-regulated in Alzheimer’s brains and localizing to astrocytes and degenerating neurons [104–109], interact with Ab and occur in amyloid plaque, ... potential and decrease cell protection by Hsp25 In another example, Hsp27 and aB-crystallin appear in Parkinson’s disease 2617 Small heat shock proteins and disease with severe dementia [119] sHSPs and ... HSPs that protect deregulated intracellular signaling proteins and transcription factors 2619 Small heat shock proteins and disease Y Sun and T H MacRae Table sHSP modifications associated with...
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Báo cáo khoa học: Functional classification of scaffold proteins and related molecules pptx
... Functional classification of scaffold proteins L Buday and P Tompa Table Scaffold proteins and their kin Representatives of the four categories of scaffold proteins and their relatives are presented ... functional description of scaffold proteins and their kin, in general, and their distinct classes, in particular, encompassing scaffolds [5], docking proteins [6], anchors [7] and adaptors (this article) ... regulating more proteins in complex ways (scaffolds ⁄ anchoring proteins) and proteins specialized to initiate signaling cascades by localizing partners at the cell membrane (docking proteins) This...
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Báo cáo khoa học: Compartmentalized signalling: Ras proteins and signalling nanoclusters pdf
... basic residues on proteins and acidic lipids in the plasma membrane Biochem Soc Symp 75, 189–198 27 Silvius JR, Bhagatji P, Leventis R & Terrone D (2006) K-ras4B and prenylated proteins lacking ... now switching towards understanding context-dependent regulation of interactions and outputs within this large signalling web Ras trafficking and localization H-, K-, and N-Ras share almost complete ... that is driven by interactions of their HVRs with membrane proteins and lipids [20–22] These nanoclusters are small (< 15 nm diameter) and short-lived (t1 ⁄ £ 0.4 s) [23] K(B)-Ras operates from...
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Báo cáo khoa học: 15 N-Labelled proteins by cell-free protein synthesis Strategies for high-throughput NMR studies of proteins and protein–ligand complexes doc
... interconversion between Ala and Glu, Glu and Asp, and Glu and Gln is efficient in wheat germ extract but can effectively be suppressed by inhibitors of transaminases and glutamine synthase [20] ... scrambling and easier sample handling compared to in vivo protein production render cell-free protein synthesis particularly attractive for high-throughput production of proteins and selective ... amino acids and the necessity to purify each individual sample In contrast, cell-free systems allow the synthesis of [15N]-labelled proteins with very small quantities of [15N]-amino acids and they...
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Báo cáo khoa học: Computer-assisted mass spectrometric analysis of naturally occurring and artificially introduced cross-links in proteins and protein complexes potx
... disulfide bonds, and artificially introduced cross-links in proteins Cross-links impose distance constraints on amino acid residues that can be used to model the 3-D structure of proteins and protein ... disulfide bond structures, chemical modifications and cross-linking in protein mixtures, complexes, and assemblies Proteins A single protein, a list of proteins from a mixture or from a protein complex ... Netherlands Organization for Scientific Research (NWO), Chemical Sciences division (CW) and Regieorgaan Genomics The ApexQ FTICR-mass spectrometer was largely funded by NWO-CW and the TofSpec 2EC and...
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Báo cáo khoa học: Comprehensive sequence analysis of horseshoe crab cuticular proteins and their involvement in transglutaminase-dependent cross-linking potx
... through Y9), and repeats of the di- and tri-peptide sequences QQ and QQQ were observed in the amino terminal sequences of basic QH proteins and 10 Nucleotide sequences of HMM chitin-binding proteins ... chitin-binding proteins Basic G13 and G19, acidic DE25 and DE29 and S37 were shown by RT-PCR to be expressed predominantly in epidermis (Fig 7) In contrast, basic Y6, Y7 and QH4 were broadly expressed, and ... in insect cuticular proteins [4] A blast homology search revealed no other regions of similarity between acidic S37 and other known proteins The members of basic Y and G, and acidic S37 all contain...
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Báo cáo khoa học: Dynamin-related proteins and Pex11 proteins in peroxisome division and proliferation doc
... domain, and the C-terminal GED (Fig 2) Obvious PRDs or PH domains are not found in DRPs Examples of DRPs are Mx proteins, mammalian DLP1, and the yeast proteins Dnm1, Mgm1 and Vps1 Mx proteins ... GIM5A and GIM5B These two proteins are nearly identical in sequence and show weak similarity to Pex11 Both are 26 kDa, have two putative 5172 S Thoms and R Erdmann transmembrane domains, and ... peroxisomal matrix and membrane proteins, yet contain vesicular structures with some peroxisomal proteins [61] Pex28 and Pex29 from S cerevisiae are also peroxisomal membrane proteins [62] They...
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Báo cáo khoa học: A novel mechanism of TGFb-induced actin reorganization mediated by Smad proteins and Rho GTPases docx
... also enhanced by overexpression of Smad3 and Smad4 proteins (3.4-fold) and to a lesser extent by overexpression of Smad2 and Smad4 proteins (2.3-fold), and this activity was stimulated further ... promoter Smad2 and Smad3 proteins activate RhoA and RhoB GTPases and induce actin polymerization and microfilament reorganization in Swiss 3T3 fibroblasts To examine whether Smad proteins, in addition ... min, and GST–RBD was used to isolate GTP-loaded RhoA and RhoB from cell lysates Both proteins were monitored by immunoblotting using anti-RhoA and antiRhoB specific serum, and the protein band...
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Báo cáo khoa học: Separation and characterization of caveolae subclasses in the plasma membrane of primary adipocytes; segregation of specific proteins and functions docx
... functional and dynamic relationships between closed Identification of caveolae subclasses and open caveolae, as well as how targeting and sequestration of the different proteins and lipids are ... (lanes b1 and b2); LD-caveolae (lane c) a, b1 and b2, and c were separately subjected to SDS ⁄ PAGE (9% acrylamide) and silver staining Indicated are apparent molecular masses of reference proteins ... of protein (lanes and 4, VHD-caveolae; lanes and 5, HD-caveolae; lanes and 6, LD-caveolae) The amount of the analysed proteins is expressed as arbitrary densitometric units and normalized to...
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Báo cáo khoa học: DNA mediated disassembly of hRad51 and hRad52 proteins and recruitment of hRad51 to ssDNA by hRad52 pot
... Disassembly and recruitment of hRad51 and hRad52 proteins shorter and thicker nucleoprotein filaments when ssDNA is added [28] Tumour suppressors BRCA2 and p53 interact with the ... was incubated in binding buffer in the absence (lanes and 3) or presence of 75 lM ssDNA (lanes and 5) and mM ATP (lanes and 5) for h at 37 °C and then subjected to partial digestion with trypsin ... lanes and with those in lanes 3, and reveals that the FEBS Journal 273 (2006) 199–207 ª 2005 The Authors Journal compilation ª 2005 FEBS 203 Disassembly and recruitment of hRad51 and hRad52 proteins...
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Báo cáo khoa học: The mystery of nonclassical protein secretion A current view on cargo proteins and potential export routes pdf
... both N- and O-glycosylated b-galactose-terminated oligosaccharide side chains of glycoproteins [9,73] as well as by galactosecontaining glycolipids such as GM1 [73,82] As galectin-1 and galectin-3 ... premature binding to glycolipids and glycoproteins within the lumen of the classical secretory pathway [9] However, in other cases it is less clear why these cargo proteins are exported by unconventional ... nuclear export and secretion Development 126, 3183–3190 111 Bianchi, M.E & Beltrame, M (2000) Upwardly mobile proteins The role of HMG proteins in chromatin structure, gene expression and neoplasia...
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Báo cáo khoa học: Cleavage of focal adhesion proteins and PKCd during lovastatin-induced apoptosis in spontaneously immortalized rat brain neuroblasts ppt
... kinase (FAK), actin-binding structural proteins, and the adaptor proteins paxillin and Crk-associated substrate (p130Cas) [17] Among these, FAK, p130Cas and paxillin tyrosine phosphorylation are ... least 11 isoforms of PKC have been identified and can be classified into conventional (a, bI, bII and c), novel (d, e, g, and h), atypical (f and k ⁄ i) and novel ⁄ atypical (l) based upon their cofactor ... per lane) were subjected to SDS ⁄ PAGE and western blotting using specific antibodies against p130Cas (A and B), PKCd (C and D, upper panel) and actin (C and D, lower panel) as described Molecular...
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Báo cáo khoa học: Modules, multidomain proteins and organismic complexity ppt
... as stand-alone domains in single domain proteins Proteins Bacteria Archaea Protozoa Plants Fungi Metazoa 273 23 16 57 20 129 a 859 728 756 620 371 881 Domains Multidomain proteinsc (% of proteins) ... (and some transmembrane) multidomain proteins has significantly expanded in metazoa [2,9], we have analyzed the size distribution of extracellular, transmembrane and intracellular multidomain proteins ... intracellular and transmembrane multidomain proteins) has revealed that extracellular domains used in the construction of extracellular proteins (and extracellular parts of transmembrane proteins) ...
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Báo cáo khoa học: The protein tyrosine phosphatase PTP-Basophil/Basophil-like Interacting proteins and molecular functions doc
... localization in epithelial cells and is accumulated in axons and growth cones of sympathetic and sensory neurons [23,28,29] Furthermore, PTP-Bas localizes to the Golgi apparatus and to the nucleus [22,30] ... distribution of PTP-Bas and Fas in Panc89 cells upon stimulation with Fas-ligand [30] Unstimulated cells showed limited colocalization of PTPBas and Fas However, upon treatment with Fas-ligand, colocalization ... 452 16 References Hunter, T (1995) Protein kinases and phosphatases: the yin and yang of protein phosphorylation and signaling Cell 80, 225–236 Andersen, J.N., Mortensen, O.H., Peters, G.H., Drake,...
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Báo cáo Y học: The amyloid precursor protein interacts with neutral lipids Liposomes and monolayer studies pdf
... molecules When APP concentrations were 0.35 and lgÆmL)1, s1 reached 200 and 23 min, while s2 was around 25 and 75 min, respectively From these results, s1 and s2 appear to be very sensitive to the ... of an electrostatic repulsion effect between lipids and the protein at physiological pH Anionic lipids, which represent % 20% of biological membrane lipids, provide either a source of electrostatic ... of the 30% layer and samples were centrifuged at 160 000 g for h at °C Fractions (0.5 mL) from the gradient were subsequently collected from the bottom of the tube and the lipid and protein contents...
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mass spectrometry of proteins and peptides
... (see Notes and 9) 3.1.1 Excision of Protein Bands (spots) from Gels Rinse the entire gel with water and excise bands of interest with a clean scalpel, cutting as close to the edge of the band as ... Direct Analysis of Proteins in Mixtures 17 Direct Analysis of Proteins in Mixtures Application to Protein Complexes John R Yates, III, Andrew J Link, and David Schieltz Introduction Tandem mass spectrometry ... Winston, S., and Hauer, C R (1986) Protein sequencing by tandem mass spectrometry Proc Natl Acad Sci USA 83, 6233–8238 Yost, R A and Boyd, R K (1990) Tandem mass spectrometry: quadrupole and hybrid...
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báo cáo hóa học:" Correlation between expression of p53, p21/WAF1, and MDM2 proteins and their prognostic significance in primary hepatocellular carcinoma" docx
... specimens and data analysis, and drafted the manuscript ZYZ and JF participated in the interpretation of data, conducted immunohistochemistry, and western blot analysis All authors read and approved ... p53, p21/WAF1, and MDM2 in HCC tissues than the corresponding adjacent hepatic tissues; the expression levels of the proteins in the former was 70.7%, 33.1%, and 52.5%, respectively and those in ... [24] and cutaneous squamous cell carcinoma [25], but is decreased in colorectal carcinoma [26] and ovarian carcinoma [27] Table 3: The expression of p53, p21/WAF1, and MDM2 in HCC tissues and...
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Báo cáo hóa học: " Respiratory syncytial virus (RSV) attachment and nonstructural proteins modify the type I interferon response associated with suppressor of cytokine signaling (SOCS) proteins and IFN-stimulated gene-15 (ISG15)" potx
... intracellular IFNα, IFNβ and SOCS1 and SOCS3 protein levels were determined at 24 h and 48 h pi by flow cytometry (Figure 2) At 24 h or 48 h pi, IFNα and IFNβ protein expression in RSV and RSV mutant virus ... cells to RSV infection and the role of RSV surface proteins in modulating this response The studies center on SOCS1 and SOCS3 negative regulation of the type I IFN response and ISG15 expression ... of SOCS3, ISG15 and IFNβ mRNA expression Results RSV stimulation of SOCS1, SOCS3, IFNα and IFNβ mRNA expression To determine the relationship between RSV infection, RSV proteins, and SOCS regulation...
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