line cell culture and phagocytosis assay
Báo cáo y học: " Comparative analysis of cell culture and prediction algorithms for phenotyping of genetically diverse HIV-1 strains from Cameroon" pps
... with10% FBS and antibiotics Appropriate controls included uninfected cells, and cells treated with co-receptor antagonists TAK 779 (9.14 μmol/ http://www.aidsrestherapy.com/content/6/1/27 ml) and AMD ... CXCR4/CCR5 Columns indicate sample ID's and their corresponding HIV genotype from partial sequences (gp41/p17/pol), its tropism by prediction and cell culture assay tributing to the evolving diversity ... potentially be an alternate tool to cell culture assays for phenotypic characterization of emerging, new and diverse HIV strains in a population where genetic diversity is high and continuing to evolve...
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Báo cáo y học: "Cell culture and passaging alters gene expression pattern and proliferation rate in rheumatoid arthritis synovial fibroblasts" pdf
... the cell suspension transferred into preheated (37°C) culture medium and centrifuged to remove the DMSO It was resuspended in culture medium and then transferred into cell culture flasks and cultured ... the cells was performed at 85 to 95% confluency as fibroblasts exhibit contact inhibition (unpublished observations) and were passaged 1:2 to provide cell- cell contacts between the cells Cell culture ... the cells were grown to 85 to 95% confluence, then trypsinized, counted and 50,000 cells were seeded into five fresh cell culture six-wells (passage 1) Each day, one well was trypsinized and...
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Báo cáo khoa học: Dual expression of mouse and rat VRL-1 in the dorsal root ganglion derived cell line F-11 and biochemical analysis of VRL-1 after heterologous expression pptx
... features of DRG cells present in F-11 cells such as l- and d-opioid receptors, receptors for prostaglandin and bradykinin, and voltage-sensitive calcium channels Also F-11 cells synthesize and release ... codon for P761 and a stop codon The accuracy of the resulting new plasmid pcDNA3.1/rVRL-1 was confirmed by DNA sequencing of both strands Cell culture and transfection of F-11 cells F-11 cells (obtained ... brain and F-11 cDNA were used On a 6% polyacrylamide gel fragments A, B and D of F-11 cells again separated into two bands The slower migrating band always corresponded to the rat brain control, and...
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cancer cell culture, methods and protocols
... dissociated cells in culture Tissue culture Maintenance of tissue explants in culture Cell line A culture that is subcultured beyond the initial primary culture phase Finite cell line A cell line with ... trypsin, breaks the cell- cell and cell- substrate links and creates a single -cell suspension Subculture of a primary culture into a secondary culture produces a cell line. ” Once a cell -line is created, ... human and animal cell lines European Collection of Animal Cell Culture (ECACC) http://camr.org.uk CAMR, Salisbury, Wilts, SP4 OJG, UK Contains over 900 human and animal cell lines Interlab Cell line...
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báo cáo hóa học: "Fibrillar beta-amyloid peptide Aβ1–40 activates microglial proliferation via stimulating TNF-α release and H2O2 derived from NADPH oxidase: a cell culture study" doc
... microglial cells were counted and their numbers expressed as percentage of cell number at time '0' The dashed line indicates cell number at time '0' Data are expressed as mean of experiments ± standard ... synthesized EUK-8 and EUK-134 and helped to write the manuscript providing useful suggestions GCB conceived of the study, and participated in its design and coordination, and wrote Introduction and Discussion ... After a 24 hour incubation, microglial cells were counted and their numbers expressed as percentage of cell number at time '0' The dashed line indicates cell number at time '0' i.e 100% Data are...
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Báo cáo y học: " High cell density and latent membrane protein 1 expression induce cleavage of the mixed lineage leukemia gene at 11q23 in nasopharyngeal carcinoma cell line" ppt
... mediated predominantly by CAD and partially by other nucleases Page of Methods Cell lines NPC cell lines SUNE1 and HONE1, as well as the EBV genome-positive marmoset cell line, B95-8 (gifts from Prof ... other hand, cells transfected with LMP1 expression plasmids (Fig 4C, lanes and 4) showed very distinct and intense bands of sizes smaller than kb DNA sequencing of these bands (600 bp and 300 ... bromide-stained agarose gel SUNE1 (lanes 1-3) and HONE1 (lanes 4-6) seeded at cell number of 0.4 × 105 (lanes and 4), × 105 (lanes and 5) and × 105 (lanes and 6) were harvested for genomic DNA extraction...
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báo cáo khoa học: "PVP-coated silver nanoparticles block the transmission of cell-free and cell-associated HIV-1 in human cervical culture" ppsx
... 0.15, 0.1, 0.05 and mg/mL) After 24 h, the medium containing PVPcoated AgNPs was removed and washed three times with culture media Cell viability was measured by the CellTiter-Glo® assay Graphs ... Richman), H9+ cells (from Dr Robert Gallo), HeLa-CD4-LTR-β-gal cells and the viral strains HIV-1IIIB and HIV-1AZT-RV HIV-1IIIB was propagated by sub-culturing in the MT-2 and H9+ cells, according ... the cell- free supernatants from virulent cultures were used for viral inoculation MT-2 and H9+ cells were cultured in RPMI 1640 (Sigma-Aldrich), supplemented with 10% fetal calf serum (FCS) and...
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báo cáo khoa học: " Embryonic stem cells in scaffold-free threedimensional cell culture: osteogenic differentiation and bone generation" ppt
... 22:138-147 30 Rando TA: Stem cells, ageing and the quest for immortality Nature 2006, 441:1080-1086 31 Gerber I, ap Gwynn I: Influence of cell isolation, cell culture density, and cell nutrition ... functions of the cells, e.g differentiation and proliferation, rely on intact cell- cell interactions and a tight attachment to extracellular matrix components In micromasses, the cells can interact ... Germany) The cells were derived from the inner cell mass of blastocysts extracted from C57BL/6 mice and tested positive for the stem cell markers Pouf1 (alias Oct4) and Foxd3 [18] Cells were cultured...
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Báo cáo khoa học: " Thermal stability and inactivation of hepatitis C virus grown in cell culture" ppsx
... cytotoxic to cells, Huh7-25-CD81 cells were treated with individual, serially diluted detergents and subjected to MTT assay to assess cell viability Based on the MTT assay results (Figure 4B and 4C), ... determined by FFU assay on Huh7-25-CD81 cells and shown as mean log10 FFU/ml ± SD The dashed line indicates the detection limit of the FFU assay (10 FFU/ml) The asterisks in (A), (B) and (D) denote ... vivo setting awaits further evaluation Methods Cell culture and virus stocks The Huh7-25-CD81 cell line (a generous gift from Dr Takaji Wakita), a Huh7 cell clone that stably expresses human CD81...
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Báo cáo y học: "Calcium deposition in osteoarthritic meniscus and meniscal cell culture" pps
... These meniscal cells were fibroblast in appearance, and there were no differences between the OA meniscal cells and the normal meniscal cells in appearance These cells produced aggrecan and type II ... was added and the cells were cultured for 24 hours Culture medium with 1% serum was changed again and cells were cultured for another 24 hours Total RNA was extracted from these cells using Trizol ... examined and compared the expression of ENPP1 and ANKH in OA meniscal cells and in normal meniscal cells Both microarray and real-time RT-PCR analyses indicated that the expression of ENPP1 and ANKH...
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Báo cáo y học: " Distinguishing between linear and exponential cell growth during the division cycle: Single-cell studies, cell-culture studies, and the object of cell-cycle research" ppsx
... Control and maintenance of mammalian cell size BMC Cell Biol 2004, 5(1):35 Prescott DM: Relations between cell growth and cell division I Reduced weight, cell volume, protein content, and nuclear ... Cooper and Moses Cooper At various times the child would stand against the board and their height would be measured by drawing a line and dating the line At the left is the full board and at ... Biology and Medical Modelling 2006, 3:10 Here I argue to the contrary, that single cell studies are more misleading than population studies Understanding cell growth should be based on cell culture...
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Phytohormones on cell growth and taxol accumulation via cell culture of taxus wallichiana zucc
... among the cultured lines than by any other factor (Bonfill M et al., 2006) Paclitaxel and baccatin III production in cell lines obtained by mixing low-, medial- and high-producing cell lines was ... paclitaxel via calli and cell suspension cultures using different explants and media (Christen et al., 1991; Fett-Neto et al., 1992; Wickremeshinhe and Arteca, 1993) Suspension cell culture was also ... callus induction, subculture, and selection of appropriate callus types; (ii) cell suspension establishment and subculture; and (iii) enhancing paclitaxel biosynthesis of cells Precisely, the...
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Cambridge.University.Press.The.Crisis.of.Literature.in.the.1790s.Print.Culture.and.the.Public.Sphere.Nov.1999.pdf
... Pete, Guy, TerryBall, James, Andy, Mick, Opera-John, Mark and Sabine, and Tim and Melinda Tarik, Ben and Guy provided an unfailing supply of beds, couches, floors and backgammon within easy range ... the social and political constraints, and above all, the intense mutualities and struggles in social space that guide and block the passage of signs among historical writers, readers and audiences.5 ... poetry in England, and you will find it accompanied with literature [England’s poets] by their literature enriched their poetry; and what they borrowed from the public stock of art and science,...
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Báo cáo y học: "Expression of Human Globular Adiponectin-Glucagon-Like Peptide-1 Analog Fusion Protein and Its Assay of Glucose-Lowering Effect In Vivo"
... incretin hormone released from islet -cell and intestinal L-cells in response to the ingestion of food [3] It plays an important role in glucose homeostasis and has shown promising effects as a ... pH 7.0) supplemented with 80mg Kanamycin /l and cultured overnight at 37°C Typically, 2mL of overnight grown culture was added to 200mL of medium and incubated with shaking at 37°C until optical ... Buffered Saline (5min each time) and then incubated with horseradish peroxidase-conjugated second antibody for 2h, washed again with Tris Buffered Saline as described previously, and finally...
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Some aspects of American culture and society in the twentieth and twenty-first centuries through a number of selected short literary works
... concepts including culture and society, literature, short stories and other genres of literature, techniques in storytelling, and short literary works and their portrayal of culture and society Besides, ... customs, laws and etc make up a culture Culture and society are closely related We not have two different societies with exactly the same culture or one society with completely different culture Let ... memory with the sights and the people and in her time, she had learnt to respect their native states and their parents and everything else On the contrary, her children and grand children did not...
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The relationship between an organization’s structure and culture and the effects on business performance
... self-image and style of the organization; its shared values and beliefs, norms and symbols”5 Each organization has different expectations, values, norms and ways to adapt and identify its culture ... Harrison, Handy classify four main types of organizational culture: power, role, task, and person culture. 6 Within power culture, power is central by judgment of key individual In role culture, ... guide to Problems and Practice Paul Chapman Veeramuthu (2008) Organizations and Behavior, Hanoi: National Economic University 2- page handout on the Organizational Structure and Culture 3 Website:...
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Workplace culture and productivity
... workplace, and thereafter test for improvements in a longitudinal study I have identified (and seek to measure and test) the following factors that that can be described as "cultural" in nature, and ... they have the tools and information correct needed decisions? Is to their information jobs and consistent make across different channels? Is policy and procedure followed and enforced evenly ... that end, and measure both productivity and morale again after a specified period "before" and of time "after" The percent measurements change would between transcend differences of scale, and multivariate...
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Accepting Command-Line Options, Switches, and Parameters
... the old and new machine names, the machine’s IP address, and perhaps the machine’s domain or subnet mask While you may want the subnet mask and domain name to be set from the command line, these ... starts this line is a valid shell command that performs no active task In this syntax it simply expands any arguments that may follow it on the line In this case we simply want to expand the value ... exit and display the variable, the line of code where it exited, and its LOGNAME substitution, as with the :? syntax username="" echo "${username?$LOGNAME}" Both the :? and ? syntaxes are excellent...
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Control of Cell Proliferation and Growth byMyc Proteins
... both cell growth and cell proliferation through multiple pathways and that there is neither a single critical effector gene nor a small group of such genes In cell culture, Myc can affect cell ... required for cell proliferation and cell growth or whether its essential role in proliferation is restricted to specific cell types Control of Cell Proliferation and Growth by Myc Proteins 331 ... targets of Myc in cell proliferation and their proposed functions Control of Cell Proliferation and Growth by Myc Proteins 333 2003) There is clear evidence that this effect is direct and not mediated...
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Tài liệu Cell Differentiation and Embryonic Development-chua doc pptx
... and other neurons, muscles and glands Those synapses that are used remain and get stronger, the other synapses detach, and the axons shrivel and die Which brain cells and which of their synapses ... becoming a cell of a particular type is called cell differentiation The zygote is a totipotent cell - its daughter cells can become any cell type As the development proceeds, some of the cells become ... distance and affect the neighboring cells, which become other parts of the eye During embryonic development, some cells migrate For instance, cells of the neural crest migrate throughout the embryo and, ...
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