... domain B) from AMY2 (in green) and TAA (in black) The superimpositioning was guided by the catalytic acids (D179AMY2, E204AMY2, and D289AMY2 and D206TAA, E230TAA, and D297TAA) The invariant Y51AMY2 ... [17] and in contrast to Tyr51AMY2 and Tyr82TAA, the geometry differed of the Met52AMY2 (Met53AMY1) and Trp83TAA (Fig 1A) Also the larger TAA loop in TAA appeared to hinder binding of the substrate ... conserved in plant a- amylases, has a role in substrate binding and activity This local region was different in structures of nonplant a- amylases having a longer ba loop which typically contained aromatic...
... pressed tothe part of the stem which was cut The angle α is the final angle of the loading inlet, which is defined by angles α1 and α2 (Mikleš et al 2004) There are many factors influencing the ... shaft torque The other rotating parts are not important due to low weight The average no-load input of the chipper was established as 4.09 kW The no-load input was evaluated and controlled individually ... cutting wood was supplied by the motor during caracole and by the rotor in consequence of the revolution decrease The torque of the rotor consists of the disc torque, shaft torque and cardan shaft...
... 305-8575, Japan Department of Rheumatology, Clinical Research Center for Allergy and Rheumatology, Sagamihara National Hospital, National Hospital Organization, 18-1 Sakuradai, Minami-ku, Sagamihara 252-0392, ... designed and coordinated the study and helped inthe manuscript preparation All authors read and approved the final manuscript Competing interests The authors declare that they have no competing interests ... related tothe induction of proinflammatory cytokines IRF5 is activated by TLR7 signaling and regulates the expression of many genes, including type I IFN and proinflammatory cytokines [19] STAT4...
... 95/98/NT/2000/XP, Linux RedHat and Solaris The Attack Planner enables the user to state plan goals, launch the planner and view the attack plans generated For example, a user can state the goal “An external ... server translates the information returned by the Sentinels into CycL and adds it tothe KB This approach is potentially vulnerable to an attack on the Sentinels themselves, or to spoofing of their ... with no initial access gains administrator/root access to target.mynetwork.net.” The user then examines the plans returned, using the browser to get more detail about any interesting or unclear steps...
... savings balance (hundreds) Active account Barangay’s distance to branch Bank’s penetration in barangay Standard deviation of balances in barangay (hundreds) Mean savings balance in barangay (hundreds) ... door -to- door visit from the Bank “Active” (row 2) defined as having had a transaction in their account inthe past six months Mean balances of savings accounts include empty accounts Barangays are ... impact using Intent to Treat specifications as well as quantile regressions, and using both change in savings balance as well as binary outcomes for increasing savings over certain percentage...
... MeMIH-B was amplified by PCR using T7 promoter-linked primers (forward, 5¢-TAATACGACTCACTATAGGTACTATG TATCGCATGCCAAT-3¢; reverse, 5¢-TAATACGACTC ACTATAGGTACTTTAAAGTCCCGGGTTGA-3¢) For PCR, the reaction ... In other words, thein vitro results indicate that MeMIH-B may act directly on the hepatopancreas and ovary to increase the rate of vitellogenin gene expression, indicating that both the hepatopancreas ... urea in binding buffer), sonicated, and agitated overnight at °C Following centrifugation as above, the supernatant was collected and loaded into an Ni2+–nitrilotriacetic acid–agarose (Qiagen,...
... on the cantilever, and over toa photodiode detector The photodiode signal is calibrated in picoNewtons When pressed against the layer of protein attached toa substrate, the cantilever tip can ... the separating b-strands is defined as the distance between the first amino acid of strand A (Y9) and the last amino acid of strand G (K87) The elongation of the x(Y9)Àx(87) distance up tothe ... length After the protein has collapsed, it acquires the final native contacts that define the native fold To confirm that the protein had indeed folded, at seconds we stretched back again at a force...
... Immunoprecipitation and SDS/PAGE Materials and cell lines The mAbs V.1 and V.5 against GPV, ALMA.12 against GPIba, ALMA.16 against GPIX and RAM.1 against GPIbb, were developed in our laboratory [19] ... enrichment in N-mannose sugars added before the medial Golgi compartment On the other hand, the 82 kDa soluble molecule was resistant to Endo-H and cleaved by neuraminidase toa 72 kDa form, indicating ... of a Ca2+ chelator, impermeable or permeable forms of calpain inhibitors (calpastatin, lactacystin), were unsuccessful and did not restore surface expression of the mature protein The similar...
... psychologist Elias Porter in an eminently sensible and approachable way The authors use a case example to illuminate fundamental concepts ina manner that is both compelling and readable A definite addition ... training and development services that incorporate the SDI are available from many individual consultants and large consulting organizations The capacity for delivery of these services can also ... the address against what was inthe e-mail from Jenny, Dr Mac’s assistant Yup Everything appeared to match up The building looked more like a hotel circa 1940, with large, stone walls that had...
... are maintained at each clinic site and include data routinely collected as part of patient care These data include basic socio-demographic, clinical, laboratory and pharmacy information for all ... responsible for the initial conception and design of the data, participated inthe data analysis and drafted the original text MAM participated inthe data analysis and made significant comments ... quality assurance Delays at any of these steps may mitigate the positive time gains resulting from the increased MD/MO availability due tothe CD4 nurse intervention Finally, having the nurse interpret...
... microarray analyses, and performed statistical analyses on microarray data JB performed final data analysis and drafted the manuscript All authors read and approved the final manuscript Acknowledgements ... Probes/Invitrogen) to label the cRNA and washed again An Axon GenePix Scanner was used to scan the microarrays GenePix Pro software (MDS, Inc., Toronto, ON) was used to acquire and align the microarray image ... contaminating genomic DNA Total RNA was eluted from the column The RNA concentration and purity were calculated using the RNA 6000 Nano LabChip in an Agilent Bioanalyzer The RNA was stored at -70°C...
... encroaching into the natural habitats of these creatures [8] Furthermore, contrary to what many people may believe, the cobra is actually not an aggressive snake and avoids encountering humans as ... research ethics board from the Advanced Dental and Medical Institute, Universiti Sains Malaysia Permission was similarly obtained from the Hospital Director to allow us to access the information ... such as muscular weakness, spreading paralysis (within 15 to h), dysphagia, dysphasia, ptosis, external opthalmoplegia as well as slowed, labored breathing, culminating in respiratory arrest with...
... graphics, charts, drawings line drawings, cartoons or any original illustrations) it must be camera ready (see page 36) Normally, finished drawings or paintings are acceptable, but cartoons ... Ethics and the law The second was the action where the Coca-Cola Corporation were also successful in forcing the supermarket chain, Sainsburys, to change the appearance the get-up of their ... own-brand cola It was alleged that confusion was being caused to customers wishing to purchase Coca-Cola because of the similarity Details of the law affecting lotteries and competitions are contained...
... the day and time you want, and can use it without interruptions Internal locations These are usually easier to arrange However, remember that lighting and other technical apparatus all have to ... ones Also, you may need special clearance, permission or a fee may be payable to use certain locations, such as a garden, an interior, the front of a building, or a building site Weather watching ... needed External locations These need advance planning in more detail Again, a reconnaissance before the day to pick suitable locations may be essential, especially if you are using several different...
... normally be ina formal setting and have a formal agenda, at least for part of the meeting The adoption of the previous minutes, chairmans address, presentation and adoption of the annual accounts ... of delegates can vary from perhaps 150 at the bottom of the scale to several thousand at the top end The average attendance is usually around the 350450 mark Dates and seasons Most major conferences ... event, but also inthe staffing and inthe implementation After all, you are presenting, or even selling, a professional service on the stand, so why so in an amateurish way? Bad planning and slipshod...
... well, is the key It can be done, and done in such a way as to turn the situation to advantage; at the very least tolimit any damage toa minimum What follows are some of the basic rules that apply ... issues being raised In addition, because many businesses now carry pages with a large amount of information instantly available to anyone who taps into it, security of information has become a major ... that, unlike the printed word, broadcast messages are somewhat difficult to retain However, they have an instant impact, eg a natural disaster such as an earthquake But because of their transience,...
... conversations the least accurate but sometimes indicative Analysis The analyst (that is the PRO) then has to examine all the material collected, analysing and classifying it so that a report can ... Conclusion The many changes in attitudes that have taken place in our society in recent years, with an increasing demand for information, the questioning of decision making and the increasingly voracious ... it may appear Analysts should take everything into account when assessing information and in their subsequent analysis again, the detective work analogy Feedback will come from a wide variety...
... can include the container, the labelling and often the packaging However, if the products are in different areas of trade then no legal action can be taken Examples of this are inthe use of the ... by making available tothe Consultancy all relevant information 117 319 app 3.p65 117 13/06/00, 12:59 Appendix Consultancy status The Consultancy acts in all its contracts as a principal at law ... contract: an offer; an acceptance; a consideration NB A one-sided bargain, such as a gift, is not a contract The offer For an offer to be made there needs to be a definite intention to create a...
... tothe agenda Any other business Any items not already on the agenda but which need to be dealt with or may be called for at the start by the chairman Date, time and venue of next meeting Agenda ... If your invitation is accepted and put inthe diary then you can draw up your draft outline programme with timings You can probably make certain arrangements in advance (who will be involved, ... tabled at the meeting Matters arising [and not dealt with elsewhere on the agenda] Topic(s) The items to be discussed or dealt with at the meeting They may be either tabled, spoken to or attached...
... that the next-of-kin have been informed before releasing any names tothe media At the finish l l Stand down Stand down the crisis team/equipment, etc gradually as the situation eases Staffing ... inthe credibility battle l Expert advice have expert advise available to deflect the media inthe early stages l Answers prepare answers to (potentially damaging) questions from the media ... secretariat Information provision Training Management backing is vital Have a good one for background information Keep it small key people only Important to maintain it Be prompt It shows you are...