li raj rajagopalan jianwen jiang mark saeys calculation of the phase diagrams of igg using a simple four site model the 4th graduate student symposium jointly organized by chbe and gpbe nus 2007

Phase separations in protein solutions a monte carlo simulation study

Ngày tải lên : 14/09/2015, 14:06

... liquid-liquid phase behavior of a non-globular protein International Conference on Materials for Advanced Technologies, 2007, Singapore Jianguo Li, Raj Rajagopalan, Jianwen Jiang, Mark Saeys Calculation ... Calculation of the Phase Diagrams of IgG Using a Simple Four- site Model The 4th Graduate Student Symposium (Jointly organized by ChBE and GPBE) , NUS 2007 xviii Chapter Introduction Chapter Introduction ... the four- site model: the star-like shape and the linear shape The full phase diagrams for the two shapes of the four- site model will be calculated, compared and discussed (2) To investigate the...

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Báo cáo y học: "Coverage and error models of protein-protein interaction data by directed graph analysis" pptx

Ngày tải lên : 14/08/2014, 08:20

... DS and DS performed the computational and statistical analyses TC, RG and WH wrote paper All authors read and approved the final version of the manuscript 10 Chiang et al R186.13 reviews AP-MS, ... proteins and CAI, we plotted diagrams of the adjacency matrix If the value of CAI is associated with the tendency of a protein to have a large number of unreciprocated edges, then we should see a pattern ... characterized by three traits: the coverage of the tested interactions, the presence of biases in the assay that systematically affect certain subsets of proteins, and stochastic variability in the measured...

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Báo cáo y học: "The retinoic acid binding protein CRABP2 is increased in murine models of degenerative joint disease" pot

Ngày tải lên : 09/08/2014, 01:22

... JR, Ayala J, Kimura JH, Williams HR, Mumford RA, Flannery CR, Carlson SS, Iwata M, Sandy JD: Cellmediated catabolism of aggrecan Evidence that cleavage at the "aggrecanase" site (Glu373-Ala374) ... GDF5 have been linked to OA, and similar to the retinoid signalling pathway, they all play a role in endochondral ossification and modulation of their activity may impact maintenance of the articular ... The surgical area was shaved and a three-part preparation was applied containing hibitane soap (Ayerst, Montreal, Quebec, Canada), isopropyl alcohol and betadine solution (Purdue Pharma, Pickering,...

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Báo cáo y học: " Characterization of protein tyrosine phosphatase H1 knockout mice in animal models of local and systemic inflammation" docx

Ngày tải lên : 11/08/2014, 03:20

... platform and a removable infrared generator (radiant heat 137 mW/cm2/s) was placed underneath the animal's hind paw The apparatus automatically detected the withdrawal of the paw Latency of each ... histological evaluation Hargreaves' plantar test Thermal hyper/hypoalgesia was assessed by Hargreaves' plantar apparatus (Plantar test, Ugo Basile, Italy) [19] The test was performed at 1, 3, and 24 ... the article A great and indispensable support to the final version of this article has been given by Daniela Boselli, Vittoria Ardissone and Chiara Ferrandi Patrignani et al Journal of Inflammation...

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Báo cáo y học: " Differential expression and function of breast regression protein 39 (BRP-39) in murine models of subacute cigarette smoke exposure and allergic airway inflammation" pps

Ngày tải lên : 12/08/2014, 13:22

... Kasinska and Sussan Kianpour, the secretarial assistance of Marie Colbert, and Gordon Gaschler for discussion and preparation of the manuscript Additional technical assistance was provided by Jagbir ... (Kinematica, Switzerland) AMCase and BRP-39 levels were assessed by enzyme linked immune-sorbent assay (ELISA) The assay utilized anti-BRP-39 or anti-AMCase monoclonal antibodies for capture and ... participated in mouse experiments and manuscript preparation MJ, AJC, and AAH participated in the design of the study helped to draft the manuscript MRS conceived and designed the study and aided...

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FOAMING PROPERTIES OF PROTEINS.pdf

Ngày tải lên : 16/12/2012, 15:10

... at a lower temperature may be related to the increased viscosity of the liquid phase and a decreased rate of protein denaturation (Buckingham, 1970; Mita et al., 1977) The rate of drainage of liquid ... foaming characteristics of protein i.e the foaming capacity of protein and the stability and strength of the foam Foaming ability i.e Gi denotes the amount of gas retained in the foam reflecting the ... Huang and John Amistoso for their assistance in the analyses of data, and to Western Wheat Quality Laboratory (USDA) for their cooperation This research was supported in part by a grant-in-aid...

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Models of motherhood in the abortion debate - self-sacrifice versus self-defence

Ngày tải lên : 01/11/2013, 08:20

... save all ice cave explorers, thereby obliterating the need to sacriWce the life of one in order to save the lives of the others, that would solve the ethical and legal complications of the sacriWce ... example of the symbiotic ideal of mother and child that cultures so often idealize However, given the quality and quantity of the transformations of a woman’s body and liberty resulting from a ... rationale for sacriWcing a member of the group disappears, and with that disappearance, the language of justiWcation for the killing of anyone or anything no longer applies This is because the...

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Tài liệu Báo cáo khoa học: Animal models of amyloid-b-related pathologies in Alzheimer’s disease docx

Ngày tải lên : 16/02/2014, 09:20

... understanding of molecular pathogenesis Today a wide range of animal models are available for mechanistic, therapeutic and functional studies They offer an appealing means to rapidly move from simplistic ... vessel walls leading to hemorrhage and perivascular microgliosis and astrocytosis The Dutch and Iowa mutations both reduce the negative charge of Ab and stimulate the formation of amyloid ﬁbrils and ... human APP are of interest because the great majority of sporadic AD patients not carry any disease causing APP mutation In early transgenic attempts, APP processing was bypassed altogether and...

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Tài liệu Báo cáo khoa học: Bacitracin is not a speciﬁc inhibitor of protein disulﬁde isomerase pptx

Ngày tải lên : 16/02/2014, 14:20

... refolding of rhodanese was also decreased by bacitracin The decrease in the noncatalyzed rate (31%) was similar to that of the PDI-catalyzed reaction (a 32% decrease) These results Table Analysis of the ... other thiol–disulﬁde exchange enzymes, Escherichia coli DsbA and DsbC, as well as the isolated catalytic a domain of PDI Both the PDI a domain and DsbA have a catalytic site, with an associated ... However, the effects of removal of EDTA and addition of bacitracin appeared to be independent of each other (Fig 2B), implying that any metal ions bound by bacitracin and taken into the reaction are...

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Tài liệu Macro-Finance Models of Interest Rates and the Economy pdf

Ngày tải lên : 17/02/2014, 03:20

... long rate minus a short rate), and curvature (e.g., a mid-maturity rate minus a short and long rate average) Another approach, which is popular among market and central bank practitioners, is a ﬁtted ... theoretical matter, asset prices and the macroeconomy are inextricably linked, as asset markets are the mechanism by which consumption and investment are allocated across time and states of nature ... it also seems likely that the recent ﬁnancial and economic crisis the Great Panic and Recession of 2008 and 2009—will both rearrange the economic landscape and a ect the focus of economic and...

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Tài liệu Báo cáo khoa học: Mitochondrial targeting of intact CYP2B1 and CYP2E1 and N-terminal truncated CYP1A1 proteins in Saccharomyces cerevisiae ) role of protein kinase A in the mitochondrial targeting of CYP2E1 pdf

Ngày tải lên : 18/02/2014, 16:20

... Zgoda VG, Murray BP & Correia MA (2005) Vacuolar degradation of rat liver CYP2B1 in Saccharomyces cerevisiae: further validation of the yeast model and structural implications for the degradation ... Omura T (2006) Mitochondrial P450s Chem Biol Interact 163, 86–93 Bhagwat SV, Biswas G, Anandatheerthavarada HK, Addya S, Pandak W & Avadhani NG (1999) Dual targeting property of the N-terminal ... mitochondria-targeting signals at the N-terminus of mammalian CYP proteins [4,10] We found that post-translational processing of CYP 1A1 at the 4th and 32nd amino acid residues by a cytosolic endoprotease...

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Tài liệu Báo cáo khoa học: Analysis of proteins and peptides on a chromatographic timescale by electron-transfer dissociation MS ppt

Ngày tải lên : 18/02/2014, 16:20

... radical anion abstracts a proton and generates a radical site that triggers dissociation to produce a complementary pair of fragment ions of type c¢ and z¢Æ Subtraction of the m ⁄ z values for the ... and protein analysis, those that trap ions by radiofrequency electrostatic ﬁelds rather than with static magnetic and electric ﬁelds High-quality ECD spectra often require the averaging of data ... the fragments within a given ion series that differ by a single amino acid affords the mass and thus the identity of the extra residue in the larger of the two fragments The complete amino acid...

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Tài liệu Báo cáo khoa học: Applications of diagonal chromatography for proteomewide characterization of protein modiﬁcations and activity-based analyses doc

Ngày tải lên : 18/02/2014, 16:20

... [39] – and describe the use of COFRADIC for studying interactions between small molecules and proteins The latter is a particular application of ‘post-translational COFRADIC’, by which a small molecule ... choice, as all N-terminal peptides isolated by COFRADIC, by the nature of the process, will end on an arginine residue This metabolic labeling introduces a mass spacing of Da between light and heavy ... equipped with an autosampler, and can be purchased from a variety of companies; and, at least in our hands, HPLC solvent gradients and ﬂow rates can nowadays be controlled such that the overall reproducibility...

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Tài liệu Báo cáo khoa học: Interferon-a induces sensitization of cells to inhibition of protein synthesis by tumour necrosis factor-related apoptosis-inducing ligand ppt

Ngày tải lên : 19/02/2014, 06:20

... effector caspases also, is enhanced in cells treated with the combination of IFNa and TRAIL, relative to TRAIL alone In view of the cleavage of caspase substrates such as BID and PARP, the effect of ... IFNa and TRAIL on the DNA content of MCF-7 cells was also assessed, using ﬂuorescence-activated cell sorting Figure shows that, in spite of the activation of caspase-8 and the cleavage of BID and ... the regulation of protein synthesis, IFNa enhances the extent of activation of caspase-8 by TRAIL in MCF-7 and HeLa cells Our data therefore suggest that the degree to which this apical caspase...

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Tài liệu Báo cáo khoa học: "Parsing with generative models of predicate-argument structure" pptx

Ngày tải lên : 20/02/2014, 16:20

... evaluation of the probabilities of the same words and if they have the same number of lexical heads, and if the ﬁrst two elements of their lists of lexical heads are identical (the same words and ... for Natural Language Parsing Ph.D thesis, University of Pennsylvania Julia Hockenmaier and Mark Steedman 200 2a Acquiring Compact Lexicalized Grammars from a Cleaner Treebank In Proceedings of the ... On the Wall Street Journal task, the overall performance of this model is lower than that of the SD model of Hockenmaier and Steedman (2002b) In that model, words are generated at the maximal...

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Tài liệu Master Thesis Health Sciences: Expert Elicitation to Populate Early Health Economic Models of Medical Diagnostic Devices in Development pdf

Ngày tải lên : 20/02/2014, 16:20

... estimations due of the limited amount of information and data available Therefore, in the analysis and interpretation of the results, much attention was paid to assess the validity and accuracy of ... 2.1.2 Behavior and mathematical approach in expert elicitation To elicit priors either a behavioral or a mathematical approach can be used Using the behavioral approach a group of experts is asked ... visualize the whole breast However, as with X-ray mammography, it does not have the ability to visualize the tissue between the breast and the thoracic wall and the axilla The prevalence of tumors...

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Tài liệu Báo cáo Y học: Prediction of protein–protein interaction sites in heterocomplexes with neural networks ppt

Ngày tải lên : 21/02/2014, 15:20

... both in the contact and in the whole surface The dependence of the accuracy values and of the fraction of total residues with a given accuracy on the reliability index [34] of the prediction are ... method and the implicit model of interaction can be tested by additional mutations, by solving the structure of some of the complexes or by other experimental means CONCLUSIONS We have analysed the ... x class with respect to the overall amount of total predictions made for that class The prediction efﬁciency has a P(x) value of 0.72 and this is by far higher than that obtained with the random...

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Tài liệu Báo cáo Y học: Uncoupling of protein-3 induces an uncontrolled uncoupling of mitochondria after expression in muscle derived L6 cells ppt

Ngày tải lên : 21/02/2014, 15:20

... genome and a lox site, and the other plasmid, pAvhUCP3lx, containing the left portion of the viral genome, the UCP3 expression cassette, and a lox site as described previously [26] Both plasmids and ... measured the membrane potential of mitochondria in situ after selective permeabilization of the plasma membrane of the cells with a mild digitonin treatment The mitochondrial potential was analysed ... recombinant adenovirus The position of the probable UCP3 band is indicated on the right side of the panel mitochondria is pushed to its maximal capability While the basal oxygen consumption of the...

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Tài liệu Báo cáo Y học: Short peptides are not reliable models of thermodynamic and kinetic properties of the N-terminal metal binding site in serum albumin doc

Ngày tải lên : 22/02/2014, 04:20

... modelling of Cu(II) and Ni(II) transport by albumins On the other hand, the direct thermodynamic and kinetic characterization of Ni(II) binding at site I in HSA and BSA was obtained These data can ... similar to each other In particular, the parameters for VIHN complexes not deviate systematically from those of DAHam and DAHKam This means that the side chain carboxylate of Asp1 does not have a ... fact, the values of kobs for HSA and BSA, very similar to each other, are intermediate between those for DAHam and DAHKam This shows that the mechanism of metal binding at site I in albumin cannot...

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Tài liệu Báo cáo Y học: Enhancement by a-tocopheryl hemisuccinate of nitric oxide production induced by lypopolysaccharide and interferon-c through the upregulation of protein kinase C in rat vascular smooth muscle cells docx

Ngày tải lên : 22/02/2014, 04:20

... not of the antioxidants BHA and AsA These results suggested that active oxygens and free radicals did not participate in the TS effect, and that the inhibitory effect of a- T was mediated by a nonantioxidative ... 381S Badamchian, M., Spangelo, B.L., Bao, Y., Hagiwara, Y., Hagiwara, H., Ueyama, H & Goldstein, A. L (1994) Isolation of a vitamin E analog from a green barley leaf extract that stimulates release ... method) A P value of < 0.01 was considered to be statistically signiﬁcant Treatment of VSMC with TS RESULTS VSMC were isolated from rat thoracic aorta using the proteases elastase and collagenase as...

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