... near to the binding site(s) and their positive charge is a stringent requisite for the binding This is not surprising, if indeed collagen binds inside the concave surface of decorin, owing to the ... collagens and positive peptides, whereas fibronectin and a 30-kDa fibronectin fragment having heparin-binding ability showed interaction with tendon decorin (Fig 4) Fibronectin is known to interact with ... decorin and collagens or their CNBr peptides is quite sensitive to the ionic strength of the buffer, suggesting an ionic character of the binding The main decorin region implicated in the binding...
... syntaxin-1A, and munc18 by CKI, CKII and PKA Casein kinase I a (CKIa, upper panel), casein kinase II (CKII, middle panel) or protein kinase A (PKA, bottom panel) were tested for their ability to phosphorylate ... phosphorylation of synaptic vesicle-associated protein SV2 by CKI modulates its ability to interact with synaptotagmin Phosphorylation of syntaxin-1A by CKII increases its ability to interact with synaptotagmin ... that CKII phosphorylates in vitro syntaxin-1A on Ser14, and that this residue is phosphorylated in vivo, it remains to be determined whether CKII is the kinase Fig Summary of phosphorylation sites...
... Specificity of cytochrome c biogenesis System II System I Disulfide isomerization Heme handling/ligation Fig Schematic representation of cytochrome c biogenesis Systems IandII The systems illustrated ... towards a diheme cytochrome c [11] In E coli, natural cytochrome c biogenesis requires at least 10 proteins, contrasting with the single protein constituting System III System II is of intermediate ... recognition or specificity of System II A particularly notable point for examination is the ability of Systems IandII to mature single-cysteinecontaining c-type cytochromes (XXXCH or CXXXH motifs)...
... Domain Database (NCBI) the similarity of the two fasciclin domains in CLAP with other fasciclin I containing proteins is clear (Fig 9) Using BLAST (http://www.ncbi.nlm.nih.gov/BLAST) to identify ... completely inactivated after h preincubation at 40 °C and pH 6.8, whereas the dimer retained about 70% of its initial activity under these conditions (Fig 4C) Similar differences in cathepsin L Early ... Artemin is an RNA-binding protein with high thermal stability and potential RNA chaperone activity Arch Biophys 424, 189–200 Ó FEBS 2004 Cathepsin L and cell adhesion protein in Artemia (Eur J Biochem...
... 0 Time (s) Fig BIAcore analysis of IGF -II and IGF -II analogue binding to highanity recombinant IGF1R Binding of IGF -II (A), des-(1±6)-IGF -II (B) and Arg6-IGF -II (C) to rhIGF1R measured by BIAcore ... deprivation-induced cell death (Fig 4E) and Arg6IGF -II and des(1±6)-IGF -II were essentially equipotent to IGF -II Leu27-IGF -II and des-(1±6,10)-Leu27-IGF -II did not exhibit any survival-promoting ... relationship (Fig 5) Thus, those IGF -I mutants designed to investigate IGFBP binding [Long IGF -I, des-(1±3)-IGF -I, Arg3-IGF -I, Gly3IGF -I, Arg6-IGF -II and des(1±6)IGF -II] have basically equal abilities to...
... mammalian HDL and its principal apolipoproteins A -I and A -II in antimicrobial or antiviral activities in vitro [13,14,30], these proteins have not been yet recognized as important effectors in innate ... apoA -II, both major apolipoproteins contribute significantly to the antimicrobial activity displayed by carp plasma HDL Results Purification of HDL and apolipoproteins A -I and A -II As shown in Fig ... apolipoproteins A -I and A -II in the in vitro antimicrobial activity of carp HDL Both proteins inhibit the Ó FEBS 2004 growth of Gram-positive and -negative bacteria, including fish pathogens, at micromolar...
... duties bring one into close touch with all sorts and conditions of men His imagination was lively and fertile, his vision kaleidoscopic, his power of observation quick and true He had a high ... tribe Lever, according to himself, found no difficulty in being admitted to Red-Indian fellowship, and for a time the unrestrained life of the prairie was a delightful and exhilarating experience ... smoking "I send you by private hand the proof of chaps, iii., iv., and v of 'Lorrequer,' and am sincerely happy to find they are to your likings, andI hope in the ensuing chapter, which I expect...
... peroxiredoxin [8] TryPs were originally identied and characterized in Crithidia fasciculata [812] and these have been subsequently identied and studied in a variety of trypanosomatids [1318] Additional ... TryX indicating that formation of an intramolecular disulde is important for interaction with TryX In contrast, this mutant displayed signicant activity with dithiothreitol (65% of wild-type) ... genomic DNA of L major Friedlin strain using forward primer containing an NdeI site and reverse primer containing a BamHI site (Table 3) The 525 bp PCR-product was digested with NdeI and BamHI and...
... strategies to optimize fecundity in artificial insemination Experimental procedures Isolation and radioiodination of PSP -I ⁄ PSP -II Fig Ribbon diagram of the PSP -I ⁄ PSP -II heterodimer showing the ... function and uterine immune activity, thus ensuring reproductive success The PSP -I ⁄ PSP -II complex contributes to maintaining sperm with high viability, motility, and mitochondrial activity [14] ... heparin-binding fraction of boar seminal plasma [26], no free PSP -II has been detected, indicating that PSP -I is synthesized in excess over PSP -II, and that the PSP -II subunit is quantitatively...
... SBE II to 64% (Fig 1B) Thus, the potato isoforms differed in that SBE I was more active than SBE II on amylose and SBE II was more active than SBE I on amylopectin, which is in accordance with ... activity on amylose and amylopectin with the starch –iodine assay Amylose (type III, Sigma) and amylopectin (Sigma) from potato were typically dissolved at 10 mg:mL21 in 0.5 M NaOH The solutions ... results obtained with maize SBE IandII [3,15,16] The lmax of the amylose substrate shifted from 616 nm to 543 nm after incubation for 180 with SBE Iand to 574 nm after incubation with SBE II for...
... Denileukin diftitox (Onzar) is a recombinant protein comprising IL-2 fused with the alpha chain of diphtheria toxin, (DAB389+IL-2), capable of transiently eliminating T regs Phase II/ III clinical ... with minimal toxicity Conclusion: Addition of IFN-α to ex vivo monocyte-derived DCs, did not significantly enhance peptide-specific T cell responses in vivo, compared with TNF-α alone Class II ... Collection (ATCC) and maintained in Iscove's Modified Dulbecco's Medium supplemented with glutamine, and penicillin and streptomycin (100 IU/ml and 100 μg/ml, respectively, Sigma-Aldrich, UK) Peptide-pulsed...
... the financial statements using City staff PART II: The Audit Services shall include an opinion on the General Purpose Financial Statements and “in relation to” opinion on individual funds and ... Proposer’s Qualifications (in addition to minimum qualification) Part I Identify the partners, managers, and supervisors who will work on the financial preparation services, including staff from ... reporting and grants Responsible for maintaining all City funds including the following: General, Parks and Tourism, Tax Increment Financing Districts (TIF and 2), Capital Improvements Program (CIP),...
... TNF on interferon-inducible gene expressions in peripheral blood mononuclear cells in vitro The expression of 15 IFI genes (IFIT1, IFIT3, IFIT5, IFI6, IFI16, IFI27, IFI30, IFI35, interferon-induced ... downregulated in vivo but was upregulated upon TNF stimulation in vitro Meanwhile, IFI genes such as IFIT1, IFIT3, OAS1, ISG15 and IRF7, and IL8RA were upregulated in vivo, but IFIT1, IFIT3, OAS1 and IL8RA ... erythematosus II: IL-12 production in vitro is correlated negatively with serum IL-10, positively with serum IFN-gamma and negatively with disease activity in SLE Cytokine 1998, 10:148-153 Calvano SE, Xiao...
... Lippiello L, Zarins A: Biochemical and metabolic abnormalities in articular cartilage from osteoarthritic human hips II Correlation of morphology with biochemical and metabolic data J Bone Joint ... expression levels of sensitive genes and be the explanation for this effect This is in line with reports of upregulation of col IIand col I synthesis in chondrocytes in response to cyclic loading in ... very likely to increase inappropriate mechanical loading in many parts of the joint and, in keeping with this, col IIand YKL40 expression rose in all locations after ACLT in our study Surprisingly,...
... TNF on interferon-inducible gene expressions in peripheral blood mononuclear cells in vitro The expression of 15 IFI genes (IFIT1, IFIT3, IFIT5, IFI6, IFI16, IFI27, IFI30, IFI35, interferon-induced ... downregulated in vivo but was upregulated upon TNF stimulation in vitro Meanwhile, IFI genes such as IFIT1, IFIT3, OAS1, ISG15 and IRF7, and IL8RA were upregulated in vivo, but IFIT1, IFIT3, OAS1 and IL8RA ... erythematosus II: IL-12 production in vitro is correlated negatively with serum IL-10, positively with serum IFN-gamma and negatively with disease activity in SLE Cytokine 1998, 10:148-153 Calvano SE, Xiao...
... disease, insulin dependent diabetes mellitus (IDDM), Addison's disease, rheumatoid arthritis, myasthenia gravis, vitiligo, alopecia, pernicious anaemia and hypoparathyroidism, but the association to ... syndrome is not definite [10] Normally Addison's disease is often associated with other autoimmune diseases, such as thyroiditis, IDDM, premature ovarian failure (POF), vitiligo and hypoparathyroidism ... in the adrenal cortex and steroid producing cells in the gonads, reflecting the risk of developing ovarian failure In addition patients with Addison's disease and POF, also show antibodies directed...
... protein of collagen III was increased in the bronchioles of smokers and in stage I- II COPD Overall, the amounts of precursors of collagen Iand III were increased in smokers and stage I- II COPD ... Iand III collagen protein precursors Immunohistochemical staining of the precursor protein of collagen Iand III was present mainly extracellularly, being visible as linear and reticular fibers ... Kaarteenaho-Wiik R, Lammi L, Lakari E, Kinnula VL, Risteli J, Ryhanen L, Paakko P: Localization of precursor proteinsand mRNA of type Iand III collagens in usual interstitial pneumonia and sarcoidosis...
... Type II cadherins result in their distinct behaviour in vivo Type I cadherins, including E-cadherin, N-cadherin and C-cadherin etc., show stronger and more rapid adhesion than Type II cadherins and ... cadherins form cadherin-catenin complex with catenins and p120, no vinculin is recruited B) In the released state, the vinculin binding site in α-catenin is inhibited by a putative inhibitory domain ... observed in the Type II cadherins artificial junction under the similar condition (40) The different clustering mechanism of Type Iand Type II cadherins may partly account for their distinct adhesion...