leukotriene lysophospholipid and cannabinoid receptors in invertebrates

Báo cáo khoa hoc:" Expression of estrogen and progesterone receptors in vestibular schwannomas and their clinical significance" docx

Ngày tải lên : 11/08/2014, 07:21

... immunofluorescence histochemical staining to detect estrogen-binding activity and found of tumors had estrogen-binding activity and the intensity of the staining was found to be much greater in the tumors from ... no competing interests Authors' contributions SJ: Principal investigator - did the study, collected data and wrote the paper; VA: Co-investigator - helped in doing the study and collecting the ... Estrogen and progestin receptors in acoustic and spinal neurilemmonas Surg neurol 1986, 26:142-148 Siglock TJ, Rosenblatt SS, Finck F, House WF, Hitselberger WE: Sex hormone receptors in acoustic...

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Báo cáo khoa học:" Expression of Human CD4 and chemokine receptors in cotton rat cells confers permissiveness for productive HIV infection" pptx

Ngày tải lên : 12/08/2014, 04:21

... expression of the receptors by incubating the cell with the chromatin relaxing agent trichostatin A (TSA, 100 ng/ml)[33] CCRT and VCRT cells stably expressing hCD4 and hCCR5 receptors were produced ... collaborators[19] detected viral cDNA in spleen and brain of all the infected animals months post-infection, showed increase in mortality (17%), and found morphological changes in cells of the CNS We have ... virus infection in macaques[27], HSV-2 infection in mice[28], gonorrhea in mice[29] In recent clinical trials sponsored by NIAID (The HPTN 035 Study of Two Candidate Microbicides, BufferGel and...

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Báo cáo khoa học: " Expression of Human CD4 and chemokine receptors in cotton rat cells confers permissiveness for productive HIV infection" pdf

Ngày tải lên : 12/08/2014, 04:21

... expression of the receptors by incubating the cell with the chromatin relaxing agent trichostatin A (TSA, 100 ng/ml)[33] CCRT and VCRT cells stably expressing hCD4 and hCCR5 receptors were produced ... collaborators[19] detected viral cDNA in spleen and brain of all the infected animals months post-infection, showed increase in mortality (17%), and found morphological changes in cells of the CNS We have ... virus infection in macaques[27], HSV-2 infection in mice[28], gonorrhea in mice[29] In recent clinical trials sponsored by NIAID (The HPTN 035 Study of Two Candidate Microbicides, BufferGel and...

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Serotonin and serotonin receptors in neural stem and progenitor cell proliferation

Ngày tải lên : 11/09/2015, 10:15

... β1-intergrin, Tenascin-R, and guidance signaling molecules, such as GABA, neuregulin and Slits These molecules maintain the stability, mobility and direction of the neuronal migration (Ming and ... presence of NSPCs in the brain comes from the discovery through thymidine-H3 incorporation into the dividing cells that the cells in certain regions of the brain can undergo proliferation and generate ... NSPC differentiation showing NSPC specific viii expression Reduction in NSPC proliferation in TPH1 KO mice further pointed to the role of TPH1 in regulating and maintaining NSPC proliferation Taken...

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Tài liệu Báo cáo khoa học: Nuclear receptors in the mosquito Aedes aegypti Annotation, hormonal regulation and expression proﬁling ppt

Ngày tải lên : 18/02/2014, 13:20

... code for a single protein Interestingly, during the initial process of identifying the NR family members in the A aegypti genome, we predicted two isoforms of AaFTZ-F1, differing only in their A ... Ets-domain protein and Broadcomplex, C2H2-type zinc-ﬁnger DNA-binding protein [18,19] Despite the achievements mentioned so far, there are additional NRs that remain uncharacterized in the mosquito, ... castaneum and A aegypti as well as the sequences of the human orthologs When different isoforms were recovered, only the longest amino acid sequences that included the DNA binding, hinge and ligand-binding...

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Báo cáo khoa học: Functional expression of olfactory receptors in yeast and development of a bioassay for odorant screening docx

Ngày tải lên : 07/03/2014, 16:20

... bioluminescence was measured following yeast stimulation by odorants in two strains expressing either I7 OR and Golf, or I7 OR and Ga15 Strains were grown in minimal media with 2% glucose (uninduced) ... that mRNA of the olfactory receptors is present in both uninduced and induced yeast cells (Fig 1) This indicates a leakage of GAL1 ⁄ 10 promoter in glucose-containing minimal medium The reporter ... of increasing amount of total proteins in membrane fractions from I7 yeast strain induced at 15 °C, and from yeast expressing SSTR2 as a control activity By taking advantage of structural and...

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Báo cáo khoa học: Soluble recombinant CD69 receptors optimized to have an exceptional physical and chemical stability display prolonged circulation and remain intact in the blood of mice doc

Ngày tải lên : 16/03/2014, 04:20

... protein unfolding, many aromatic amino acids forming the protein core become exposed with the concomitant increase in the molar extinction coefﬁcient of the protein, and thus the increase in absorbance ... protein is shown in Fig 3A indicating good dispersion of the backbone and side-chain signals (the latter including those assigned to tryptophane indole groups in the 5594 Fig Structure determination ... CD69 in buffers with neutral or slightly alkaline pH under conditions compatible with binding of potential ligands We succeeded in crystallizing the very stable CD69NG70 protein using l-arginine...

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Báo cáo khoa học: Arthropod nuclear receptors and their role in molting pptx

Ngày tải lên : 23/03/2014, 04:20

... also contain a carboxy-terminal extension containing T-box and A-box motifs [124] In each zinc finger, four invariant cysteine residues coordinate tetrahedrically to a zinc ion, and both zinc fingers ... overlapping ligand-binding pockets The difference of the binding pocket is easy to understand by looking at the superimposition between PonA and BYI06830 shown in Fig Ligand-binding affinity Typically, ... for retaining the ligand-binding activity They found two high-affinity binding sites (Kd1 = 0.24 nm and Kd2 = 3.9 nm) The removal of GST had no effect on PonA binding, but altered DNA binding The...

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Báo cáo khoa học: Membrane compartments and purinergic signalling: P2X receptors in neurodegenerative and neuroinﬂammatory events pdf

Ngày tải lên : 30/03/2014, 02:20

... iceberg in purinergic physiopathological mechanisms Understanding the entire purinergic signalling machinery, also comprising additional P2/P1 receptors, enzymes and transporters for purinergic ligands ... were found in vivo at the protein level in dopaminergic, GABAergic neurons or astrocytes Moreover, dopamine denervation obtained by unilateral injections in the rat brain of 6-hydroxydopamine (used ... huntingtin protein and the death of the neurons in the neostriatum (resulting in the appearance of generalized involuntary movements), is not fully understood According to experimental evidence indicating...

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Báo cáo khoa học: Expression and functional characterization of P2Y1 and P2Y12 nucleotide receptors in long-term serum-deprived glioma C6 cells ppt

Ngày tải lên : 30/03/2014, 08:20

... nucleotide receptors is divided into two major classes [5]: P1, responding to adenosine; P2, responding to ATP, ADP, UTP and UDP P2 receptors are further divided into two groups [6,7]: P2X, the intrinsic ... twice in NaCl ⁄ Pi, subsequently P2Y1 and P2Y12 in serum-deprived glioma C6 suspended in a DNA staining solution (75 lgÆmL)1 propidium iodide and 50 lgÆmL)1 RNase A in NaCl ⁄ Pi), and incubated ... phosphatidylinositol 3-kinase signaling with increased invasiveness and gelatinase activity in malignant gliomas J Neurosurg 95, 480–488 Vajkoczy P, Goldbrunner R, Farhadi M, Vince G, Schilling L,...

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Báo cáo khoa học: Cloning of type 1 cannabinoid receptor in Rana esculenta reveals differences between genomic sequence and cDNA pot

Ngày tải lên : 30/03/2014, 08:20

... Alignment between R esculenta brain and testis cDNA revealed two nucleotide differences in codons 186 (GGG in testis and GGA in brain) and 252 (CTC in testis and CTA in brain) Such modiﬁcations were ... long Interestingly, only alterations in codons 70 and 408 are effective in changing amino acid residues A70 and E408 are located in the ﬁrst extracellular domain, just in the conserved N-linked ... underlined ^The conserved lysine in the third transmembrane domain (TMD3) °°Leucine and alanine pair in the C-terminus of the third cytoplasmic loop implicated in the interaction with Gs in rat;...

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Báo cáo khoa học: Calcium and polyamine regulated calcium-sensing receptors in cardiac tissues doc

Ngày tải lên : 31/03/2014, 01:20

... calcium, gadolinium and spermine induced changes in intracellular calcium concentration Elevating [Ca2+]o from mM to > 1.5 mM evoked an increase in intracellular calcium concentration in more than ... spermidine and putrescine, are involved in the synthesis of nucleic acids and proteins in eukaryotic and prokaryotic cells They play an important role in the regulation of cellular proliferation and ... concentration-dependent increase in intracellular calcium (Fig 4D) With mM Ca2+ in the bath solution, spermine from 1–10 mM induced a time- and concentration-dependent increase in intracellular calcium...

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Báo cáo y học: "Differential Constitutive and Cytokine-Modulated Expression of Human Toll-like Receptors in Primary Neutrophils, Monocytes, and Macrophages" docx

Ngày tải lên : 08/08/2014, 16:23

... regulate expression of TLRs in leukocytes, including pathogen structures and TLR ligands, such as araLAM and LPS, and pro-inflammatory cytokines, including IL-1β, TNF-α, GM-CSF and IFN-γ.[16, 22, 37, ... controlled by proinflammatory cytokines and increased responses to TLR ligands GM-CSF was shown to play an important role in inflammatory signaling in a model of LPS-induced lung injury Pretreatment ... had increased TLR4 expression and also increased responses to LPS, as measured by NF-κB DNA binding activity and cytokine production (e.g., TNF-α).[39] Neutrophils had increased protein and mRNA...

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Báo cáo y học: "yclooxygenases and prostaglandin E2 receptors in growth plate chondrocytes in vitro and in situ – prostaglandin E2 dependent proliferation of growth plate chondrocytes" ppsx

Ngày tải lên : 09/08/2014, 08:22

... prostaglandin synthesis, acts locally on target cells by binding to prostaglandin E (EP)type G protein-coupled receptors Four different EP receptors are known, which are linked to different intracellular ... hormones, 1,25 (OH)2D3 and parathyroid hormone, is dependent on an increase in intracellular calcium and activation of protein kinase C [21] On the other hand, an increase in intracellular cAMP concentration ... formation and promotes DNA and matrix synthesis in growth plate chondrocytes [7] In addition to various findings in vitro, the physiological role of prostaglandins was clarified by its stimulating...

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Fibroblast-like synovial cells from normal and inflamed knee joints differently affect the expression of pain-related receptors in sensory neurones: a co-culture study ppsx

Ngày tải lên : 09/08/2014, 10:20

... bradykinin (BK) and substance P (neurokinin (NK1) receptors) In lumbar DRGs but not in cervical DRGs from AIA rats we found a pronounced increase in the proportion of neurones exhibiting BK receptors ... immunoreactivity (IR) after culturing in normal medium, in medium with the COX inhibitor indomethacin (1 μmol/l), in medium containing an anti-IL6 antibody (1 μmol/l) or in medium containing normal rat IgG ... hours in DMEM containing 100 ng/ml NGF maintained at 37°C in a humidified incubator gassed with 5% CO2 in air As a control, either only DRG neurones or only FLS cells were cultured and handled in...

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Báo cáo y học: "γ Activating and inhibitory Fcγ receptors in rheumatoid arthritis: from treatment to targeted therapies" potx

Ngày tải lên : 09/08/2014, 10:20

... augment inflammation and joint destruction in arthritic conditions In inflamed RA synovial tissue, macrophages and dendritic cells have now been shown to express FcγRIIb in addition to activating ... activating FcγR-expressing cells and T cells in RA synovial tissue [1] By enhancing the capacity of effector T cells to activate B cells as well as fibroblasts and osteoclasts (and macrophages and ... Fc-sialylated IgGs in intravenous immune globulin preparations (from 1% to 2%, to 20%) resulted in a 10-fold increased capacity to suppress experimental arthritis Based on these findings, receptors for...

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Báo cáo y học: "Toll-like receptors and NOD-like receptors in rheumatic diseases" pdf

Ngày tải lên : 09/08/2014, 14:21

... (nucleotide binding site or NACHT domain) central to the molecule, flanked by a leucine rich-repeat domain at the Cterminus and a caspase-recruitment domain and a pyrin domain at the N-terminus The ... syndromes in humans, including Mucke–Wells syndrome, chronic infantile cutaneous neurologic articular syndrome and familial cold-induced autoinflammatory syndrome [8] Fever, joint pain and systemic inflammation ... clinical and histological characteristics of arthritis (right-hand side) Histological images of knee joints are shown, stained with hematoxylin and eosin Arrow indicates inflammatory cell influx...

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Báo cáo y học: "Type I interferon receptor controls B-cell expression of nucleic acid-sensing Toll-like receptors and autoantibody production in a murine model of lupus" doc

Ngày tải lên : 09/08/2014, 14:22

... of IFN and other proinflammatory cytokine signaling, including suppressor of cytokine signaling (SOCS1) and the Tyro-3, Axl, and Mer (TAM) receptors, have been shown to associate with, and regulate ... bind to the IFN-I receptor (IFNAR), which is composed of two chains: IFNAR1 and IFNAR2 The IFNAR2 chain exists in both transmembrane and soluble isoforms and is critical for ligand binding and ... induces the expression of TLR7 and TLR9 in B cells, resulting in the activation of autoreactive B cells and in autoantibody production in vivo This response is completely dependent on signaling...

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Báo cáo y học: " mGluR5 positive modulators both potentiate activation and restore inhibition in NMDA receptors by PKC dependent pathway" docx

Ngày tải lên : 10/08/2014, 05:21

... responses [15,25] CHPG, directly binding to glutamate binding site, and DFB as well as CDPPB, binding to the heptahelical transmembrane domain of mGluR5, increase the intrinsic efficacy of the endogenous ... HCl DFB was dissolved in DMSO, whereas ketamine was dissolved in saline Then the individual reagents were diluted in an artificial cerebrospinal fluid (ACSF) containing (in mM) NaCl (120), KCl ... Electrophysiological recordings For electrophysiological recordings, the MED probe containing the hippocampal slice was placed in a small incubator which was superfused with ACSF in 5% CO2/ 95% O at 34°C and connected...

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Báo cáo y học: "The role of toll-like receptors in acute and chronic lung inflammation" pdf

Ngày tải lên : 11/08/2014, 03:20

... intact HA in order to maintain cell integrity and decrease lung injury, but also mediate macrophage inflammatory responses to HA fragments following chemically induced tissue injury [72,80] Infectious ... signaling: Death due to infection Host recovery and protection from reinfection EXCESSIVE innate immune signaling: Death due to inflammation Figure Innate and adaptive immunity in acute and chronic ... cytoplasmic signaling machinery [5] MyD88 was initially identified as part of the interleukin (IL) -1R and IL-18R signalling pathways and was subsequently implicated in signalling by almost all...

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