... modulated by lumican-evoked signaling, including vinculin and focal adhesion kinase [82] Lumican alters the relationship between actin filaments and b1 integrin, which in turn would affect focal adhesion ... protein ⁄ protein interactions Second, many surface receptors are made up of protein modules that are often shared by extracellular matrix proteins, including leucine- rich repeats, fibronectin and ... detail In analogy to decorin, lumican inhibits tumor cell growth in soft agar by increasing the expression of the cyclin-dependent kinase inhibitor p21WAF1 [72] Again, similar to decorin, these...
... system is involved in the appearance of free [35S]sulfate in the culture medium, tendon cultures were maintained in DMEM containing 10 mM ammonium chloride following days in culture in DMEM alone ... 35S-labelled large proteoglycans (d) and 35S-labelled small proteoglycans (s) remaining in the tissue at each time after incubation of bovine tendon with [35S]sulfate was determined as described in Results ... 35S-labelled proteoglycans remaining in the matrix of tendon cultures maintained in DMEM On the days indicated, tissue samples from the experiment described in Fig were extracted with M GdnHCl and aliquots...
... Vigorous was defined as activity leading to sweating or dyspnoea, and examples such as swimming, tennis, netball, athletics (which may involve running, walking, throwing or jumping), and running were ... including cartilageandbone Moreover, our recent longitudinal data have suggested that increased patella bone volume may be advantageous to patellofemoral joint health, as increased baseline bone ... included males and thus expanded the generalisability of our findings Subsequently, the present study substantiates previous findings pertaining to both men and women In the past and present studies,...
... the hands, wrists, and distal part of the feet were obtained at baseline and after 12 and 24 months Forty joints in the hands and feet were scored (in the hands, proximal interphalangeal joints ... HRT, in line with our previous findings of an increase in insulin-like growth factor in the HRT group [41] PICP correlated significantly with CTX-I and COMP and inversely with bone mass in the ... of bone turnover, in accord with the findings of Cortet and co-workers [40] BSP accounts for about 10% of the noncollagenous proteins inboneand is in particular enriched at cartilagebone interfaces...
... Vitamin E aChange (mm3) in tibial cartilage volume per standard-deviation increase in vitamin C/vitamin E intake before and after adjusting for energy intake, age, gender, body mass index, and ... body mass index, and tibial cartilage volume cChange in tibial plateau bone area (mm2) per standard-deviation increase in vitamin C/vitamin E intake before and after adjusting for energy intake, ... gender, and body mass index dOdds ratio of tibiofemoral bone marrow lesions being present per standard-deviation increase in vitamin C/vitamin E intake before and after adjusting for energy intake,...
... Vigorous was defined as activity leading to sweating or dyspnoea, and examples such as swimming, tennis, netball, athletics (which may involve running, walking, throwing or jumping), and running were ... including cartilageandbone Moreover, our recent longitudinal data have suggested that increased patella bone volume may be advantageous to patellofemoral joint health, as increased baseline bone ... included males and thus expanded the generalisability of our findings Subsequently, the present study substantiates previous findings pertaining to both men and women In the past and present studies,...
... HEXXHXXGXXH) and a conserved methionine (Met-turn) supporting the catalytic zinc Interaction between cysteine–zinc maintains proMMPs in an inactive state by preventing a water molecule from binding to ... (GAIP)-interacting C-terminus ⁄ synectin and calcium ⁄ calmodulin-associated serine kinase, since the C2 region contains a class II PSD-95 ⁄ Discs-large ⁄ Zonula occludens protein-binding motif FXF, ... (Pro) containing a cysteine switch motif PRCGXPD (except for MMP23 which lacks the cysteine switch motif) that maintains them as inactive zymogens, and a catalytic domain with a zinc-binding site...
... Accumulating evidences indicate that HSPGs act to inhibit cellular invasion by promoting tight cell–cell and cell–ECM interactions, and by maintaining the structural integrity and self-assembly of ... heparanase in human cancer by focusing on head and neck carcinoma and multiple myeloma as examples of solid and hematological malignancies Heparanase in head and neck carcinoma: signaling in motion ... protein-coding genes contain introns that are removed in the nucleus by RNA splicing and are often alternatively spliced Alternative splicing increases the coding capacity of the genome, generating...
... joints other than the index (affected) joint(s), and might in fact also originate from diverse tissue sources, including skin, intervertebral disc, meniscus and tendon [6,11-13] Notwithstanding ... 178VFSG , and revealing a practicable new signature ‘beacon’ of SLRP catabolism Recombinant MMP-13 was shown to degrade biglycan and fibromodulin (and to a lesser extent decorin and lumican) incartilage ... described work, Monfort and colleagues [1] demonstrate that MMP-13 cleaves at Gly177-Val178 within the leucine- rich region of biglycan, generating fragments bearing the amino-terminal ‘neoepitope’...
... frozen in liquid nitrogen within 30 of resection and stored in the vapor phase of liquid nitrogen Tissue sections were made from each sample, stained with hematoxylin and eosin (H&E), and examined ... in PBS containing 0.1% Tween-20 Mouse mAb 2E3 (Abnova) against rLRG1 was added at a 1:50 dilution in blocking buffer and incubated overnight at 4°C Cells were washed and incubated in a 1:50 dilution ... 14 20 Cummings C, Walder J, Treeful A, Jemmerson R: Serum leucine- rich alpha2-glycoprotein-1 binds cytochrome c and inhibits antibody detection of this apoptotic marker in enzyme-linked immunosorbent...
... differences in safranin O staining intensity between BMP-2-exposed cartilageand controls were observed by mere visual investigation Therefore, the safranin O staining intensity was scored in patellar and ... Ad-luc injection The area of the cartilage staining positive was determined with a computerized imaging system BMP-2 was compared to controls and shows an increase in VDIPEN staining on days andin ... stimulation also leads to increased VDIPEN staining (k) and NITEGE staining (q,p) Arrows point to intense staining around chondrocytes FastG, fast green; SafO, safranin O Co-incubation of several...
... proteins [1-4] Genes encoding nucleotide binding site -leucine rich repeat (NBS-LRR) proteins are the most prevalent and can be divided into two major groups based on the encoded N-terminal domains ... Columbia Landsberg Landsberg Landsberg Landsberg Landsberg Landsberg Landsberg Columbia Columbia Columbia Leaf Flower – shoot Root Seed Seed Seed Seed Seedling Seedling Seedling Seedling Seedling Seedling ... searched in 2002 for insertions in NBS-LRR-encoding and related genes using BLAST Ten enhancer trap lines and three gene trap lines were identified with insertions into NBS-LRR-encoding or related...
... (%) In further study, heavy metal leaching into rain water with lower pH value and longer soaking time should be investigated; the use of mixing treated sludge with raw material/powder for making ... concentration of Cr, Ni and Fe in water increased with the soaking time, particularly after 2days From day 14 to 21 the increase seems to be slight in comparison with the beginning days 284 N.T Ha ... in \ Heavy metal rich sludge Dehydration (dry at room temperature and at 1050C-1100C) Cooling Treating and Grinding Fine screening (d ≤ 0,05 mm) Reuse Fig Diagram of thickened sludge treatment...
... proline -rich and glutamine -rich N-terminus, which is important for transactivation, is shown in yellow, and contains the PY-motif (green) The helix–span–helix domain at the C-terminus shown in ... mechanism during unfavorable conditions [35,36] Little is known about the interaction of AP-2 and its binding partners incartilage However, at least CBP ⁄ p300-interacting transactivator with ED -rich ... has been detected in the developing olfactory bulb, neural tis- sue, especially the midbrain and hindbrain [7,14], and hypertrophic chondrocytes during chondrogenesis [15] Winger et al [16] analyzed...
... 1-6 main chains and 1-4 branchings, and the more common 1-3 main chains and 1-6 branchings.18 This fraction also is highly active in vitro andin animal models of cancer, although activity in these ... hospitals in Shanghai, Beijing, and other areas in China, with a total 189 patients The double-blind study used the same doses and test materials as the previous study, and examined PSP against the ... certainly clinically meaningful Used singly or in combination, PSK and PSP have the potential to extend survival It may prove instructive, for proponents and skeptics alike, to conduct an integrative...
... machinery is pre-existing in the cells and deadenylates IFN-b mRNA as soon as its synthesis is induced by stimulating agents The fact that the 65-kDa protein binds ARE and CRID in UV-crosslinking ... number, HTB-113) were maintained in DMEM containing 10% of fetal bovine serum (FBS; Myoclone Super Plus, Life Technologies) and 1% of penicillin/ streptomycin The cells were infected by addition ... both noninfected and infected cells [13], further supports the involvement of this protein in the deadenylation process of IFN-b mRNA Interestingly, IFN-b ARE does not recruit other ARE-binding factors...
... V S Anand et al interaction domains, including N-terminal ankyrin repeats, a leucine- rich repeat region, and a C-terminal WD40 domain It also contains a GTPase domain composed of Ras ... and C-terminus of Roc (COR) regions and a kinase domain Mutations linked to PD are found throughout the protein, including the kinase domain (G2019S and I2020T), the Roc– COR domain (R1441C and ... protein 90 is critical for maintaining the stability of leucine- rich repeat kinase J Neurosci 28, 3384–3391 Gandhi PN, Wang X, Zhu X, Chen SG & Wilson-Delfosse AL (2008) The Roc domain of leucine- rich...
... maintained in DMEM alone However, in cultures maintained in DMEM containing 100 and 1000 lgÆmL)1 heparin, the percentage of 35S-labeled proteoglycans remaining in the matrix after 10 days in ... tendon cultures maintained in the Table Incorporation of [3H]serine into macromolecules of bovine tendon explants maintained in DMEM containing varying concentrations of CaPPS or heparin The proximal ... 3481 Inhibition of proteoglycan catabolism in tendon T Samiric et al small proteoglycans remained in the matrix that had been maintained in DMEM alone, but in cultures maintained in DMEM containing...
... the maintenance of bone mass in rodents and humans We reported that ArKO mice exhibit marked bone loss due to increased bone resorption, and that the treatment with E2 restored the bone mass in ... chemicals and phytoestrogens with estrogen receptor b Endocrinology 139, 4252–4263 19 Shilling, A.D & Williams, D.E (2000) Determining relative estrogenicity by quantifying vitellogenin induction in ... was to examine the in vivo estrogenic effects of BPA on the female reproductive tract andbone by using ArKO mice MATERIALS AND METHODS Materials A standard rodent chow (NMF) was obtained from...
... no infrared anisotropy in (c) the axis of the alpha-helix [26], as shown in Figure 7a Since the amide bonds are fixed in the peptide chains and the fibril contains three identical chains in a ... anisotropy in the regular/parallel/cross sections of tendon andcartilage Since the infrared absorption of the amide I and amide II bonds has been found to have distinct anisotropy in articular cartilage ... tendon andcartilage from all different surfaces of the 3D tissue cube were investigated using infrared imaging To the best of our knowledge, there has been no study in literature regarding the infrared...