... Recognizing indicators in discourse Interpreting text by going outside it Identifying the main point or important information in a piece of discourse Distinguishing the main idea from supporting ... use when reading in their native language to reading in a language they are learning Instead, they seem to think reading means starting at the beginning and going word by word, stopping to look ... Giving Guiding Questions Giving guiding questions is a good way Apart from providing students the primary notions about the reading text, stimulating student's brains in thinking and finding...
... Publishing House of Ho Chi Minh, Vietnam, 1999-2000 (in Vietnamese) [3] Forest Inventory and Planning institute, Vietnam Forest Trees, Agriculture Publishing House, Hanoi, 1971-1988 (in Vietnamese) ... Asteraceae is the 2nd largest family in the studies carried out in Yen Tu and Huu Lung and is the 6th largest in Cat Ba In contrast, in the studies of the Yen Tu, Huu Lung and Cat Ba areas, the ... containing the highest number of species in this study and the other studies are listed in Table The 10 genera containing the highest number of species vary significantly However, each list includes...
... cells of the mouse In this way, any desired mutation can be introduced at will in haploid cells in the about 4800 non-essential yeast genes In the remaining about 1200 “essential” yeast genes, the ... essential during evolution for survival of the species, because wild yeasts for the larger part of their life (for instance during winter in moderate climates!) are actually in non-growing (starving) ... discussed in a subsequent Chapter by Jazwinski, this volume.) After this introductory discussion of the two aging models of yeast and the in uence of caloric restriction on yeast aging, we may again...
... of protein utilizing the principle of protein–dye binding Anal Biochem 2, 248–254 Bauer R & Rupe CO (1971) Use of syringaldazine in a photometric method for estimating ‘free’ chlorine in water ... variations in identity (for example ranging from 38–86% in Coprinopsis cinerea [27]) This natural diversity within in a single organism can be used for protein engineering purpose In Trametes ... sequence domain (blue copper-binding domain) that shares distant homology to the single-domain cupredoxins [21,22] The evolutionary path from a single-domain cupredoxin to a threedomain laccase...
... aprotinin, leupeptin and pepstatin, twice in kinase buffer, and used for in vitro kinase assays In vitro kinase assays Recombinant human protein kinase CK2 holoenzyme (0.5 U, corresponding to ... N-Terminal domain; B, Rio1 family-specific domain (R-domain); CK, serine-rich acidic domain (pos 402–435, CK2 domain); and K, lysine-rich C-terminal domain (K domain, pos 436–484) (B) N- and C-terminally ... chaperonin-harbouring plasmid pREP4-groESL [33], served for recombinant expression of Rio1 proteins YEp351 or YEp352 [34] were used for protein expression in yeast strains WCG-4a (obtained from...
... least in duplicate for two independent cultures of the same yeast Enzymatic assays Strains were grown in 500-mL shaking flasks containing 100 mL mineral medium [50] supplemented with 0.1% yeast ... catabolism in yeast be seen in Fig that, in C arabinofermentans PYCC 5603T, d-xylose has a stronger inhibitory effect in the putative l-arabinose ⁄ proton symporter than d-glucose In fact, radioactive ... 3593 L-Arabinose transport and catabolism in yeast C Fonseca et al Table Kinetic parameters for enzymes in the initial L-arabinose catabolic pathway Cells were grown in 2% (w ⁄ v) L-arabinose medium,...
... mycin A This drug is a well-known inhibitor of electron transfer from quinone to cytocrome b [38] Yeast strains were cultivated in aerobic conditions on plates containing rich or synthetic minimal ... so-called Pasteur effect [3] Such an inhibitory effect of oxygen could be a more recently acquired trait, originating independently in several 984 yeast lineages In contrast, whereas the upper four ... with N2 (containing less than p.p.m O2) at a flow rate of 0.1 LÆmin)1 per liter of medium, and a stir rate of 500 r.p.m was maintained In order to minimize the diffusion of oxygen into the bioreactor,...
... Mg2+-permeable ion channel [14] Using in vitro chemical cross-linking and in vivo split-ubiquitin assays to analyze protein–protein interactions, we show here that Alr1p and Alr2p interact to form homo-oligomeric ... overexpressing N-terminally deleted Alr1 proteins in an ALR1 wild-type strain Protein–protein interactions detected by the split-ubiquitin system To test for possible Alr1p–Alr1p interaction, ... construct in medium lacking methionine, we observed good growth of cells expressing NubG-ALR1 in combination with MetALR1-Cub on selective medium This strongly indicated interaction of Alr1 proteins in...
... non-agricultural income generating engaged in by rural dwellers in Yewa area, Ogun State 3.2.1 Agricultural Income Generating Activities Types of agricultural income generating activities, degree involvement ... result in rural dwellers/farmers spending more to maintain their health More money is spent to provide the things the industries should have provided our communities (spent more to get safe drinking ... result in rural dwellers/farmers spending more to maintain their health More money is spent to provide the things the industries should have provided our communities (spent more to get safe drinking...
... cystein, methionine and serine (Fig 2) Mutants containing asparagine, aspartic acid, glycine, lysine or valine were nonfunctional (Fig 2) Clones expressing eEF2 in which the adjacent threonine ... Ammerer G (2001) Rck2, a member of the calmodulin-protein kinase family, links protein synthesis to high osmolarity MAP kinase signaling in budding yeast Proc Natl Acad Sci USA 98, 5625–5630 Dunand-Sauthier ... to bind to ribosomes in cells also expressing wild-type eEF2 from one of the remaining eEF2 coding genes Because binding of eEF2 to the ribosome is dependent on the preformation of a guanosine...
... Kokubo T (2001) Mutations in the TATA-binding protein, affecting transcriptional activation, show synthetic lethality with the TAF145 gene lacking the TAF N-terminal domain in Saccharomyces cerevisiae ... of the main factors involved in this process is the SAGA complex [34], up to now mainly connected to the initial steps of transcription We assayed our reporters in two mutants affecting subunits ... factors in Saccharomyces cerevisiae Genes Dev 13, 686–697 38 Tran HG, Steger DJ, Iyer VR & Johnson AD (2000) The chromo domain protein Chd1p from budding yeast is an ATP-dependent chromatin-modifying...
... of protein utilizing the principle of protein-dye binding Anal Biochem 72, 248–254 19 Guarente, L (1983) Yeast promoters and lacZ fusions designed to study expression of cloned genes in yeast Methods ... allowing recombination resulting in restoration of a replicating plasmid Positive recombination was identified by selection for LEU2 and GFP fluorescence In frame recombination of GFP C-terminal ... protein Cells were fixed in 70% ethanol for DAPI staining and imaged as described in Experimental procedures Fig In uence of Sut2p on invasive growth, FLO11 expression and Ras protein level in R1278b...
... uninduced and induced yeast cells (Fig 1) This indicates a leakage of GAL1 ⁄ 10 promoter in glucose-containing minimal medium The reporter plasmid pRHF-luc was cotransformed in the yeast strains ... I7 OR in yeast (A) Luciferase bioluminescence was measured following yeast stimulation by odorants in two strains expressing either I7 OR and Golf, or I7 OR and Ga15 Strains were grown in minimal ... as a function of increasing amount of total proteins in membrane fractions from I7 yeast strain induced at 15 °C, and from yeast expressing SSTR2 as a control activity By taking advantage of...
... However, preliminary docking calculations have shown that an inverted position is possible with the S-ring, rather than the N-ring, inserted into the substrate access channel Determining the structure ... should be recognized in this context that there is very little published information in the literature that would allow mapping of the amino acids with which imidazolinones interact Indeed, the literature ... DNA sequencing using the Prism Ready Dye Terminator Cycle Sequencing Kit and DNA Sequencer 373A (Perkin-Elmer Applied Biosystems) Protein purication and enzyme assays The hexahistidine-tagged...
... acid into PtdIns in an environment in which arachidonic acid is limited? In this study, we investigated the composition of PtdIns from brain, heart, liver, spleen, kidney and ovary in Seriola quinqueradiata, ... PtdIns origin [25] In general, lipids from terrestrial mammals are rich in n)6 series PUFAs, such as linoleic acid and arachidonic acid In contrast, the predominant PUFAs in lipids from marine ... docosahexaenoic acid in these tissues In striking contrast, larger amounts of arachidonic acid existed in PtdIns from all tissues investigated (Fig 1) The proportion of it in PtdIns was highest in ovary...
... reveals five ORFs, including a gene for a yeast ribokinase Yeast 6, 521–534 65 Lecain, E., Chenivesse, X., Spagnoli, R & Pompon, D (1996) Cloning by metabolic interference in yeast and enzymatic ... production [14] Protein partners involved in the yeast SCC system localize to three distinct subcellular compartments To gain a deeper insight into how the artificial steroid pathway is coordinated with ... overproduction results in an increased level of free sterol (Fig 4B), and it would also be expected to increase the cycling of sterols by esterification and hydrolysis, resulting in an increase in intracellular...
... final purpose is using data in raster format. 3.1.1. Creating the map of origins According to the data interpolation in AccessMod, map of origins contain information of population in ... Maximum travel time is defined as the travel time of local people in order to travel from origin to destination. As it was mentioned in some documents, average maximum travel time is 60 minutes. In Yen Chau District, there are two kinds of ... origins (local settlements) and destinations (public services). According to Moseley, M.J., [7], there are three components of accessibility, including: ‐ People, who is living in the study area; ...
... [2] Luyen Nguyen Ngoc, Doan Dinh Lam et all., Study a scientific aspects and measures for exploration karst cave potential in Tam DiepYen Mo, Ninh Binh, Kenh Ga-Van Trinh, Van Long, Ninh Binh for ... purpose In order to evaluate tourism potential of Ninh Binh’s caves system for tourism planning and development in the next future, during 2005-2007 Ninh Binh Tourism Department and Institute ... Mich cave (Photo Doan Dinh Lam, 2006) Fig.21 Curtains in OngMich cave (Photo Doan Dinh Lam, 2006) 3.5 Cave Ma Tien Location: Dong Thai, Yen Dong, District Yen Mo, Ninh Binh 200.04’, 32/1050 57’,...
... polymorpha The finding that H polymorpha Inp2 interacted with Myo2 points to a conserved function for this protein as a binding factor for Myo2 Remarkably, in H polymorpha, Myo2–Inp2 binding was dependent ... inheritance (involving Myo2, Inp2, Inp1, Pex3 and Pex19), in which peroxisomes are faithfully inherited into the newly formed bud (3a) and ⁄ or are retained in the mother cell (3b) during cell division ... shown to be involved in regulating peroxisome numbers [43] Peroxisome inheritance During vegetative reproduction of wild-type yeast cells, organelle replication is essential for maintaining the organelle...