lattice interactions in metallic nanoparticles
Tài liệu Báo cáo khoa học: Transient RNA–protein interactions in RNA folding docx
... electrostatic interactions [60] (d) For the Transient RNA–protein interactions in RNA folding human mRNA-binding protein hnRNP A1 [61], the Xenopus laevis protein X1rbpa [54], the trypanosome guideRNA-binding ... RNA–protein interactions in RNA folding M Doetsch et al Tat peptide induced a change in the population of coexisting and interchanging RNA conformations The lack of intermolecular NOE connectivities indicated ... molecules during their folding process or during their assembly into RNP complexes Static versus transient interactions RNA folding reactions can be modulated either by tight binding to proteins, establishing...
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Tài liệu Báo cáo khoa học: Cobalamin uptake and reactivation occurs through specific protein interactions in the methionine synthase–methionine synthase reductase complex docx
... cob(I)alamin from CH3-H4-folate, generating H4-folate and the methylcob(III)alamin form of MS During primary turnover, the homocysteine-binding domain (dotted barrel) and the CH3-H4-folate binding-domain ... form of hMS During reactivation of hMS, the FMN domain of MSR (light grey) and the C-terminal activation of hMS (grid-barrel) interact with the cobalamin-binding domain For more information on ... plates containing 100 lgÆmL)1 zeocin Several transformed colonies were streaked onto plates containing 1000 lgÆmL)1 zeocin to select for colonies containing multiple copies of the integrated cDNA...
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Tài liệu Báo cáo khoa học: The role of electrostatic interactions in the antitumor activity of dimeric RNases docx
... the hinge loop after ligation Also in this case no clashes were detected at Electrostatic interactions and antitumor RNases the dimer interface of the models or in the surroundings of the hinge ... homologous to the RNases investigated in this study, have been isolated from human eosinophils, namely eosinophil-derived neurotoxin (EDN) and eosinophil cationic protein (ECP) They are RNases ... cationic proteins, with high isoelectric point values Thus we investigated the effects Table Natural and engineered RNases investigated in this study IC50 is the RNase concentration producing half-maximal...
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Managing Drug Interactions in the Treatment of HIV-Related Tuberculosis docx
... disease 8, Predicting Drug Interactions Involving Rifamycins Knowledge of the mechanisms of drug interactions can help predict the likelihood of an interaction, if that specific combination of drugs ... rifampin induces the activity of the CYP2C19 and CYPD6 isozymes The rifamycins vary in their potential as CYP450 inducers, with rifampin being most potent, rifapentine intermediate, and rifabutin ... K, Gerstoft J, Pedersen C Pharmacokinetic interaction between rifampin and the combination of indinavir and low-dose ritonavir in HIVinfected patients Clin Infect Dis Feb 2004;38(3):426-429 Ribera...
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Báo cáo Y học: Probing intermolecular protein–protein interactions in the calcium-sensing receptor homodimer using bioluminescence resonance energy transfer (BRET) potx
... repeatedly in studies of the oligomerization of GPCRs and other protein–protein interactions [34–40] In these studies, BRET has been measured using Rluc and enhanced yellow fluorescent protein (EYFP) ... AT1aD359-GFP2 displayed similar binding characteristics in a [I125]angiotensin II whole cell binding assay (data not shown) These observations are in excellent agreement with the findings of another group ... alternate interpretation of the lack of agonist-induced BRET observed in this study is that the translation of agonist binding to the ATDs of the family C GPCR homodimer into G-protein coupling of...
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Báo cáo khoa học: Supramolecular calsequestrin complex Protein–protein interactions in chronic low-frequency stimulated muscle, postnatal development and ageing pot
... the CSQ-binding element JUN, show decreased protein–protein interactions during fiber type shifting and the ageing process Reduced protein coupling between the major elements regulating Ca2+homeostasis ... heterogeneous protein–protein interactions Slow CSQ might be involved in a more indirect type of physiological coupling process in transformed fibers, while fast CSQ appears to be a directly interacting endogenous ... changes in protein interactions between other SR Ca2+-regulatory proteins might also be involved in triggering impaired triadic signal transduction resulting in a progressive functional decline of...
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Báo cáo khoa học: Nature, nurture and neurology: gene–environment interactions in neurodegenerative disease docx
... expansions, encoding polyglutamine tracts in different proteins, suggesting that these diseases may involve overlapping molecular mechanisms of pathogenesis involving toxic gainof-function of ... made in identifying genetic factors contributing to PD in recent years [135] Low concordance for clinical disease in monozygotic twins indicates environmental in uences on PD [136], and the finding ... causing HD: an expanded CAG repeat in the gene encoding a protein that came to be known as huntingtin [5] Normal individuals have 10–34 CAG repeats in this gene 2348 T L Spires and A J Hannan Individuals...
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Báo cáo khoa học: Netropsin interactions in the minor groove of d(GGCCAATTGG) studied by a combination of resolution enhancement and ab initio calculations pot
... However, it is interesting that the guanine amino group may be in uenced by this contact We compared the interaction energies in both a planar and a nonplanar guanine G9 amino group The HF interaction ... in the planar guanine amino group was computed to be 55 kJÆmol)1 (40 kJÆmol)1 in the nonplanar guanine amino group), 25 kJÆmol)1 in total interaction energy (7 kJÆmol)1 in the nonplanar guanine ... Effects of minor groove binding drugs on the interaction of TATA box binding protein and TFIIA with DNA Biochemistry 33, 7033–7040 Welch JJ, Rauscher FJ & Beerman TA (1994) Targeting DNA-binding drugs...
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Báo cáo " Synthesis and characterisation of metallic nanoparticles " ppt
... then resuspended in PBS with pH 7.4 containing 0.2 % bovine serum albumin (BSA) Trastuzumab was covalently linked to amino-GNPs through EDC connection Briefly, the above prepared amino-GNPs was reacted ... linkages The procedure consists of the following steps [14]: Functionalizing gold nanoparticles with 4-ATP (C6H7NS) Different volumes of 4-ATP mM were added into 20 ml of the gold NPs containing ... atoms in NPs which can result in the weak magnetic iron oxides and iron hydroxides This is in agreement with the suggestion of separated Fe and Pt domains in as-prepared NPs After annealing the...
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Báo cáo khoa học: Protein interactions in the sumoylation cascade – lessons from X-ray structures pdf
... Ala72 in Nedd8, and Arg72 in ubiquitin (highlighted in yellow in Fig 2C) Matching E1 residues are Thr149 in uba2, Arg190 in uba3 and Gln608 in uba1 (highlighted in purple in Fig 3E) Interestingly, ... SP-RING domain, a SUMO interaction motif (SIM) and a C-terminal domain (CTD) [39] (B) Pc2 contains an uncharacterized E3 domain, a ubc9 binding domain and a Pro-Ile-Asp-Leu-Ser motif involved in substrate ... domain [1,42,44,45] Except for the SP-RING domain, no homology has been found between PIAS proteins and single-chain ubiquitin E3 ligases NSE2a and TOPORS, two other proteins that contain RING...
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Báo cáo khoa học: Probing protein–chromophore interactions in Cph1 phytochrome by mutagenesis potx
... other protein– bilin interactions, as studies with blocking reagents and of autoassembly kinetics have implied [15–17] In free PCB at neutral pH the two central ring nitrogens share a single proton, ... expressed as C-terminally His6-tagged apoproteins in E coli, purified and tested for PCB-binding, apoprotein folding, Pr–Pfr photochromicity and thermal reversion using SDS ⁄ PAGE ⁄ zinc fluorescence ... module (the N-terminal chromophore binding PAS ⁄ GAF domains), the missing PHY domain precluding the formation of bona fide Pfr There may also be other problems in extrapolating from 1ZTU to the...
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Báo cáo khoa học: Dissecting the role of protein–protein and protein–nucleic acid interactions in MS2 bacteriophage stability potx
... protein–RNA interactions in virus stability is not completely understood, and we have investigated these questions in this work We sought to determine the role of protein–RNA and protein–protein interactions ... covalently link them To facilitate purification, both dlFG and a single-chain dimer, sc-dlFG, contain an N-terminal six-histidine nickelaffinity tag Using intrinsic fluorescence of Trp residues, extrinsic ... conserved arginine-rich RNA binding motif in cowpea chlorotic mottle virus capsid protein results in virion structural alterations and RNA packaging constraints J Virol 79, 3277–3288 Dertinger D,...
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Báo cáo khoa học: Redox-sensitive loops D and E regulate NADP(H) binding in domain III and domain I–domain III interactions in proton-translocating Escherichia coli transhydrogenase potx
... Characterization of single-cysteine mutations in ecIII Determination of protein concentration and substrate content Protein concentrations were determined using the bicinchoninic acid assay with bovine serum ... important in NADP(H)-binding and possibly also coupling to proton translocation [4,13] The corresponding regions in ecIII are indicated in an NMR-derived structural model (Fig 1) and in the amino-acid ... two subdomains A and 1B [5] The 1B subdomain has a Rossman fold responsible for binding NAD(H) in a novel mode as compared to other NAD(H)-binding enzymes [5] By using NMR in combination with...
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Báo cáo sinh học: "Enhanced presentation of MHC class Ia, Ib and class II-restricted peptides encapsulated in biodegradable nanoparticles: a promising strategy for tumor immunotherapy" docx
... microscopy using the coumarin-6-containing NP revealed an intense cytoplasmic fluorescence (6coumarin) in the DC (Figure 2), confirming that our peptide-containing NP are avidly internalized ... important role in antitumor immunity [15,16] Therefore, it is important to examine whether CTL can be effectively primed using nanoparticle containing class Ib-binding peptides To engineer our nanoparticle-based ... after a 1-h incubation with free coumarin-6 (B), or with NP containing coumarin-6 (C) NP-loaded imDC incubated with Hoechst nuclear stain (D) Magnification 400× size distribution of the nanoparticles...
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báo cáo hóa học:" Enhanced presentation of MHC class Ia, Ib and class II-restricted peptides encapsulated in biodegradable nanoparticles: a promising strategy for tumor immunotherapy" doc
... microscopy using the coumarin-6-containing NP revealed an intense cytoplasmic fluorescence (6coumarin) in the DC (Figure 2), confirming that our peptide-containing NP are avidly internalized ... important role in antitumor immunity [15,16] Therefore, it is important to examine whether CTL can be effectively primed using nanoparticle containing class Ib-binding peptides To engineer our nanoparticle-based ... after a 1-h incubation with free coumarin-6 (B), or with NP containing coumarin-6 (C) NP-loaded imDC incubated with Hoechst nuclear stain (D) Magnification 400× size distribution of the nanoparticles...
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Báo cáo hóa học: " Atomic Force Microscopy Study of Protein– Protein Interactions in the Cytochrome CYP11A1 (P450scc)-Containing Steroid Hydroxylase System" docx
... to inoculate a preculture The bacteria were allowed to grow in ampicillin-containing nutrient broth medium at 37°C overnight These cultures were used to inoculate l of a main culture containing ... and dried in airflow The binary complexes were obtained by mixing 10 μl of μM solutions of appropriate individual proteins in 50 KP, pH 7.4 Then, the mixture was incubated for 10 min, diluted ... determined using the software Biaeval 4.1 Averaged binding curves for the interaction between Ad and varying CYP11A1 concentrations were fitted simultaneously using the 1:1 Langmuir-binding model...
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Báo cáo hóa học: " The Study of Quantum Interference in Metallic Photonic Crystals Doped with Four-Level Quantum Dots" pot
... Controlling spontaneous emission by using quantum optics would lead to several interesting effects, such as optical gain enhancement [20] and photoluminescence enhancement [21], optical switching ... the PBG can be determined by selection of proper thicknesses and refractive indices Since we are interested in optical frequencies, the radius of the metallic spheres and lattice constant of the ... the PC consisting h of spherical metal nanoparticles in a dielectric background (n2 = 1.5) has been illustrated in Fig Active Medium Embedded in a MPC The nano-sized active medium in the 3D MPC...
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Báo cáo hóa học: " Magnetic Anisotropic Energy Gap and Strain Effect in Au Nanoparticles" docx
... wavefunctions, shown in Fig 5b In a positive strained e [ nanocrystalline system, shown in Fig 5c, the lattice expands and decreases the coupling of wavefunctions In the case of our Au nanoparticles, the ... solid lines indicate the results from the fit of Eq 5; the fitting parameters are listed in Table 1; the energy gap obtained from the fit is plotted with the diameter in the Fig 5a (right panel) In ... nearest-neighbor vector The lattice constants for Au face-centered cubic nanoparticles with 123 Fig a Plots of the variation in D and strain e with mean diameter, revealing the increase in magnetic anisotropic...
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Báo cáo hóa học: " Atomistic Origin of Rate-Dependent Serrated Plastic Flow in Metallic Glasses" pot
... atomic clusters The figure indicates that the degree of atomic rearrangement underlying flow in an individual loading step varies with strain rate or loading timescale This kind of discrete flow event ... deformation indicated by (X Y-1) [rn(t - Dt)r0(t - Dt)] during the time interval [t - Dt, t] [29] We calculated Dmin values of all atoms during each displace˚ ment interval (0.1 A) to get information ... basin induced by loading is schematically shown in Fig 4b When transiting from one energy basin to another, the 123 528 Fig (a) The potential energy versus displacement curves at different strain...
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Báo cáo hóa học: " Multi-Stable Conductance States in Metallic Double-Walled Carbon Nanotubes" ppt
... irregular, having different periodicities in different parts and being asymmetric with VD, which points to a non-ideal Fabry–Perot interference pattern due to minor disorder or inhomogeneity ... and larger (0.54 nm) spaces also in the same DWCNT [30] Calculations also indicated that the interwall interactions can induce electron transfer from outer tube to interwall region, and then the ... inner tubes The continuously switching between two electronic states can be attributed to the locally orientational depinning or melting of a small part of inner tube at certain temperature (TOM,...
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