lacock abbess of d after 1280

Báo cáo khoa học: "Reduction of D-dimer levels after therapeutic administration of antithrombin in acquired antithrombin deficiency of severe sepsis" pps

Ngày tải lên : 12/08/2014, 22:22

... [9] demonstrated that elevations in soluble fibrin, fibrin(ogen) degradation products, and D- dimer often precede overt DIC by several days The D- dimer levels continue to rise thereafter as DIC ... Controlled and experimental observations in both humans and animals indicate that the elevation of fibrin(ogen) degradation products (FDP) and D- dimer is a constant feature of DIC, with marked elevations ... and hyperfibrinolysis accompanying DIC, which would be reflected by decreased levels of D- dimer In the present study, we report on changes in levels of D- dimer in response to administration of...

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Tài liệu Báo cáo khoa học: Cosubstrate-induced dynamics of D-3-hydroxybutyrate dehydrogenase from Pseudomonas putida ppt

Ngày tải lên : 18/02/2014, 16:20

... substrate-binding loop was ordered in the absence of NAD+ and disordered in the complex structure with bound NAD+ In addition, a crystal structure of a human cytosolic HBDH 5768 (DHRS6) with bound NAD+ ... structure of 5774 Fig Dynamic domains of P putida D- 3hydroxybutyrate dehydrogenase (PpHBDH) on the basis of a comparison of conformers ID and IIA, which contain a fully deﬁned substrate-binding loop ... binding of the substrate and release of the products This ﬂexibility is demonstrated by a partial disorder of the substrate-binding loop in some subunits of NAD+-bound subunits of PpHBDH and also...

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Tài liệu Báo cáo Y học: Uncoupling of protein-3 induces an uncontrolled uncoupling of mitochondria after expression in muscle derived L6 cells ppt

Ngày tải lên : 21/02/2014, 15:20

... coupled device (CCD) camera Extraction of membrane bound proteins L6 cells were harvested and membranes prepared as described above Proteins were extracted from the membranes as described previously ... re-energised mitochondria Finally, addition of the K+ ionophore valinomycin fully depolarized mitochondria This control experiment showed that mitochondria remained functional after skinning of the ... skinned L6 cells, the effect of exogenously added GDP on the membrane potential of mitochondria could be investigated Figure 5B shows that mmol concentrations of GDP did not affect the membrane...

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Báo cáo " Study on the characteristics and catalytic properties of Pt/SBA-15 in the selective oxidation of D-Glucose " potx

Ngày tải lên : 05/03/2014, 11:21

... maintained at and air flow was controlled by Flow Meter 110 AC device The products of the oxidation was determined by High performance liquid chromatography method (HPLC) with refractive index detection ... aggregated outside the channels of SBA-15 with size of 10-15nm and catalytic activities of these materials decreased pH value also affects directly on the selectivity of gluconic acid product and the ... conversion of D- glucose in to lactic acid and gluconic acid Applied catalysis A: general 343 (2008), p 49-54 [2] A.Abbadi, H.Van bekkum., “Effect of pH in the Pt catalyzed oxidation of D- glucose to Dgluconic...

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Báo cáo khoa học: Insulin resistance in human adipocytes occurs downstream of IRS1 after surgical cell isolation but at the 1 level of phosphorylation of IRS1 in type 2 diabetes pot

Ngày tải lên : 07/03/2014, 16:20

... receptor as described in various in vitro systems and models of insulin resistance [36–40] Lowered expression of IRS1 in adipocytes has been described in some obese individuals or relatives of diabetes ... Zarnowski MJ, Cushman SW & Lodish HF (2003) Standard isolation of primary adipose cells from mouse epididymal fat pads induces inﬂammatory mediators and down-regulates adipocyte genes J Biol Chem ... been described in some obese individuals and relatives of patients with diabetes [19] Animal studies have also indicated a role for IRS1 in insulin resistance in adipose tissue (reviewed in [20,21])...

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Báo cáo khoa học: Optimization of D-amino acid oxidase for low substrate concentrations – towards a cancer enzyme therapy pdf

Ngày tải lên : 16/03/2014, 02:20

... observed with free reduced DAAO B Fig Oxidative half-reaction of wt-DAAO and m-DAAO (A) Time course of the (re)oxidation of reduced enzyme followed at 455 nm upon mixing of $ 12 lM reduced m-DAAO ... mm sodium chloride, mM EDTA, 40 lgÆmL)1 lysozyme, and lgÆmL)1 DNase I) The activity was assayed by addition of 100 lL of 90 mm d- Ala, 0.3 mgÆmL)1 o-dianisidine and unit of horseradish peroxidase ... 100 lL of 0.5 mgÆmL)1 thiazolyl blue tetrazolium bromide was added; after h at 37 °C, the liquid was removed, 100 lL of dimethylsulfoxide was added, and the absorbance at 600 nm was recorded The...

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Báo cáo khoa học: Netropsin interactions in the minor groove of d(GGCCAATTGG) studied by a combination of resolution enhancement and ab initio calculations pot

Ngày tải lên : 16/03/2014, 22:20

... orientations A side-by-side binding with a guanine base was found in d( CGTATATACG) [17] and a novel end-to-end binding of two Nt molecules was determined for d( CCCCCIIIII), d( CBr5CCCCIIIII) and d( CCCBr5CCIIIII) ... novel off-centered binding with a hydrogen bond between the drug and a CG base pair Structure determinations of the same ˚ ˚ decamer with distamycin (Dst) at 2.38 A and 1.85 A, revealed two : binding ... ﬂoor of the minor groove was believed to prevent binding and sequence-speciﬁc hydrogen bonding, the detailed nature of several of the amidinium and guanidinium end contacts were investigated furFEBS...

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Báo cáo khoa học: Characterization of D-amino-acid-containing excitatory conotoxins and redeﬁnition of the I-conotoxin superfamily pot

Ngày tải lên : 16/03/2014, 22:20

... Stimulating electrodes were located in wells and 2, and a ground electrode in well Recording electrodes were located in wells and Peptide was added by exchange of the fluid contents of well To eliminate ... pair of electrodes located in wells and At the same time, the activity of the muscle was recorded with a pair of electrodes: one electrode was located in the middle, and the other at one end, of ... presence of reduced and oxidized glutathione with a yield of 70%, and purified to homogeneity by RP-HPLC The identities of the peptides were confirmed by MALDI MS, and the molecular masses of the synthetic...

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Báo cáo khoa học: De-regulation of D-3-phosphoglycerate dehydrogenase by domain removal ppt

Ngày tải lên : 17/03/2014, 11:20

... of the NSD:317 enzyme was confirmed by gel filtration The chromatographs of PGDH (predicted m 176 kDa) and NSD:317 (predicted dimeric m 68 kDa, predicted tetrameric m 136 kDa) revealed that both ... D- 2-hydroxyacid dehydrogenase of similar fold, D- LDH (predicted dimeric m 74 kDa) [19] (1ldh) The D- LDH elution profile indicates that this enzyme forms both a dimer (majority) and a tetramer [19], ... domain; SBD, substrate binding domain; and RBD, regulatory binding domain The arrows describe the positions of twofold rotation axes in the plane of the drawing The third dyad associated with the...

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Báo cáo khoa học: Enhancement of intracellular concentration and biological activity of PNA after conjugation with a cell-penetrating synthetic model peptide docx

Ngày tải lên : 23/03/2014, 13:20

... energy-dependent and -independent mechanisms contribute differently to the cellular uptake of the naked PNA and of the conjugate suggests that distinct modes of nonendocytic uptake should be operative ... could be detected In this case exclusively the naked PNA generated by cleavage of the disulﬁde bond in the reducing environment of the cell interior was found The quantity of cell-associated PNA ... the other hand, energy-dependent and -independent mechanisms contributed differently to the cellular uptake of the naked PNA and of the conjugate (Fig 3), suggesting that distinct modes were functioning...

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Báo cáo Y học: Two 1 : 1 binding modes for distamycin in the minor groove of d(GGCCAATTGG) docx

Ngày tải lên : 24/03/2014, 04:21

... direction and G11–G20 on strand 2, and the drug is labeled D After determination of the weighting factor and positional adjustment of parts of the DNA structure, water molecules were added, but not ... involved Inﬂuence of the binding of distamycin on the DNA conformation One of the most important effects of drug binding on the DNA conformation is the widening of the minor groove as illustrated ... pyramidalization Thus the DNA structure adapts to host the drug molecule, including a modest amino-group pyramidalization The contacts of the atoms O1 (D) and N1 (D) at the other side of the drug...

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Báo cáo khoa học: Crystal structures of open and closed forms of D-serine deaminase from Salmonella typhimurium – implications on substrate speciﬁcity and catalysis pptx

Ngày tải lên : 28/03/2014, 22:20

... Activity of dissolved SeMetDSD crystals without (C1) and with (C2) added PLP (50 lM); mM D- Ser was used as the substrate (B) Activity of WtDSD and SeMetDSD under different conditions as described in ... C-terminal hexa-histidine tag were not included in the model due to absence of a well-deﬁned electron density In SeMetDSD, 94.4% and 4.7% of residues were in favoured and additionally allowed regions, ... Stereodiagram of the active site geometry in WtDSD (dark grey) and SeMetDSD (light grey) PLP -D- Ser modelled in SeMetDSD is also shown The carboxyl group of modelled PLP -D- Ser is held by hydrogen...

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Báo cáo khoa học: On the reaction of D-amino acid oxidase with dioxygen: O2 diffusion pathways and enhancement of reactivity docx

Ngày tải lên : 28/03/2014, 23:20

... reduced DAAO (E-Flred, generated by reacting oxidized DAAO with a small excess of d- alanine), and (b) the reduced DAAO–imino acid complex (E-Flred–IA generated as above but in the presence of ... E-Flred and the E-Flred–IA complex for O2-induced re-oxidation (Scheme 1) A B C Discussion Fig Oxidative half-reaction of wild-type and variants of DAAO (A) Comparison of time courses of the ... labeled in bold IA: imino acid product (imino pyruvate) modeled into the DAAO active site (PDB code 1c0p) as described previously [16] Models of DAAO mutants were prepared using VMD [31] Molecular...

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Báo cáo khoa học: Catalytic activation of human glucokinase by substrate binding – residue contacts involved in the binding of D-glucose to the super-open form and conformational transitions ppt

Ngày tải lên : 30/03/2014, 04:20

... and side-chain dihedral angles of Glc contact residues in wild-type hGK on binding Glc Values are calculated from the coordinates of the super-open (PDB identity: 1v4t) and fully-closed (PDB identity: ... (PDB identity: 1v4s) has revealed that Glc is hydrogen bonded to amino acids in the L-domain (residues N204, D2 05, N231, E256 and E290) and the S-domain (residues T168 and K169) (Fig 1B) To identify ... analyses of the unliganded and Glc-bound hGK [12] have conﬁrmed the biochemical and biophysical studies by demonstrating that the binding of Glc at the active site indeed induces a large-scale domain...

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Báo cáo khoa học: Biosynthesis of D-arabinose in mycobacteria – a novel bacterial pathway with implications for antimycobacterial therapy pdf

Ngày tải lên : 30/03/2014, 04:20

... d- arabinose dehydrogenase to 2694 d- arabinitol, and the latter compound is oxidized to d- xylulose by a NAD-dependent d- arabinitol dehydrogenase d- Xylulose can then be phosphorylated to d- xylulose ... enzyme composed of two types of polypeptide that are annotated as an oxidoreductase and a short-chain dehydrogenase ⁄ reductase, and encoded by the Rv3790 and Rv3791 genes, respectively, of the M ... mycolic acids could be detected as well Subsequent analysis of the monocharged glycolipids by fastatom bombardment mass spectrometry demonstrated the presence of decaprenyl-phospho-pentoses and decaprenyl...

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awareness of deficit after brain injury clinical and theoretical issues jan 1991

Ngày tải lên : 11/06/2014, 09:45

... case of lack of awareness of left-sided motor and visual disorders was later described by Pick (1898) His patient, Adolph W., a 31-year-old alcoholic, had a cerebrovascular accident that resulted ... would have had a different meaning if applied to disorders having different degrees of severity Medium or severe anosognosia related to motor impairment (scores and 3, as defined in the preceding ... be dissociated It was noted in the preceding section that denial of severe left hemianopia (irreducible despite the examiner's demonstration of the disorder) may not be associated with denial of...

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Báo cáo sinh học: " Involvement of PKR and RNase L in translational control and induction of apoptosis after Hepatitis C polyprotein expression from a Vaccinia virus recombinant" doc

Ngày tải lên : 19/06/2014, 08:20

... incubation, the medium was removed and 50 µCi of [35S] Met-Cys Promix per mL in Met-Cys-free DMEM was added for an additional 30 minutes The cells were washed with PBS and treated as described above Immunoblotting ... action and apoptosis are defective in mice devoid of 2',5'-oligoadenylate-dependent RNase L Embo J 1997, 16:6355-6363 Der SD, Yang YL, Weissmann C, Williams BR: A double-stranded RNA-activated protein ... total RNA was fractionated in 1% agarose-formaldehyde gel and stained with ethidium bromide Abundant ribosomal RNAs 28S and 18S are indicated B and C: PKR knockout (PKR-/-) and PKR WT cells (PKR+/+)...

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Báo cáo hóa học: " Rehabilitation of gait after stroke: a review towards a top-down approach." doc

Ngày tải lên : 19/06/2014, 08:20

... INTRODUCTION Stroke is one of the principal causes of morbidity and mortality in adults in the developed world and the leading cause of disability in all industrialized countries Stroke incidence ... oxyHb and deoxyHb changes during left hand and right hand motor execution and imagery The results of signal analysis indicated distinct patterns of hemodynamic responses which could be utilized in ... types of locomotor tasks at different speeds using a treadmill During the acceleration periods immediately preceded reaching the steady walking or running speed, the levels of oxyHb increased, but...

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báo cáo hóa học: " Overexpression of serine racemase in retina and overproduction of D-serine in eyes of streptozotocin-induced diabetic retinopathy" docx

Ngày tải lên : 19/06/2014, 22:20

... and thereby contributes to the etiology of DR Therefore, we sought to determine whether production of SR and its product, D- serine, change in a model of DR utilizing the STZ-induced rat model of ... expression in retinas of STZ-induced DR model Previous studies have indicated that RGC death in DR may be associated with excitotoxicity [14,34] Recent reports have indicated that D- serine can contribute ... retinas of DR rats The top images depict hematoxylin and eosin-stained cryosections of retinas of control (A) and DR rats (B) months after onset of diabetes The cells of the GCL are uniformly distributed...

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báo cáo hóa học: " Elimination kinetics of diisocyanates after specific inhalative challenges in humans: mass spectrometry analysis, as a basis for biomonitoring strategies" doc

Ngày tải lên : 20/06/2014, 00:20

... interpretation of the data, the peak areas of individual analyzed amines were divided by the peak areas of individual standards Using this quotient the amine concentrations were estimated with standard curves ... further details of the methods, validation and controls and materials) Data analysis The excretion of the isocyanate diamines was expressed as median values ± SD (standard deviation) of the respective ... generation of diisocyanate (HDI, MDI, TDI, NDI or IPDI) standard Budnik et al Journal of Occupational Medicine and Toxicology 2011, 6:9 http://www.occup-med.com/content/6/1/9 atmospheres was performed...

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