0

keywords tcm cratoxylum cochinchinense mangiferin lipid peroxidation advanced glycation end products dna damage peroxynitrite 3 nitrotyrosine oxidative stress ca2 influx

Folk medicine  cratoxylum cochinchinense  antioxidant but cytotoxic

Folk medicine cratoxylum cochinchinense antioxidant but cytotoxic

Thạc sĩ - Cao học

... Cratoxylum cochinchinense, mangiferin, lipid peroxidation, advanced glycation end products, DNA damage, peroxynitrite, 3- nitrotyrosine, oxidative stress, Ca2+ influx, mitochondrial depolarization, potassium ... 71 3. 4 Iron-binding activity of TCM extracts 73 3.5 Inhibition of protein glycation (advanced glycation end products (AGE) formation) by TCM 3. 6 Identification of mangiferin (MGF) by high performance ... dependent DNA damage 50 2.2. 13 Peroxynitrite scavenging assays 50 2.2. 13. 1 Synthesis of Peroxynitrite 50 2.2. 13. 2 Assessment of pyrogallol red (PR) bleaching by peroxynitrite (ONOO-) 2.2. 13. 3...
  • 200
  • 289
  • 0
Isolation, purification and detection of soyasaponins and their associated bioactivities in cultured hepatocarcinoma cells

Isolation, purification and detection of soyasaponins and their associated bioactivities in cultured hepatocarcinoma cells

Cao đẳng - Đại học

... 33 3. 2.5 Flow cytometry cell cycle analysis 34 3. 2.6 Confocal observation and examination 34 3. 2.7 Statistical Methods 35 3. 3 Results 36 3. 3.1 ... and B 36 3. 3.2 HPLC-MS 37 3. 3 .3 Cell cycle distribution 39 3. 3.4 Cell morphology 42 3. 4 Discussion 42 3. 5 Conclusion ... flour 30 3. 2.1.2 Preparation of concentrated soyasapogenol A (SG-A) and B (SG-B) extracts 31 3. 2.2 HPLC and ESI-MS analysis 32 3. 2 .3 Cell culture 33 3. 2.4...
  • 147
  • 280
  • 0
Báo cáo khoa học:

Báo cáo khoa học: "Isolation and identification of Escherichia coli O157:H7 using different detection methods and molecular determination by multiplex PCR and RAPD" docx

Báo cáo khoa học

... Sources A1 A2 A3 A4 C1 C2 C3 C4 C5 C6 P1 P2 P3 P4 P5 P6 P7 438 88 438 89 438 92 438 94 29 (4-FS) 40 ( 139 8) 41 (9 73) 42 (75) 43 (796) 44 (1489) 30 09-88 (3D) 30 77-88 (3E) 31 04-88 (3C) 32 99-85 (3A) C7-88 ... P010726- 23; lane 5, P010726-24; lane 6, P010726-25; lane 7, P010726-26; lane 8, E010206- 13- 2; lane 9, J01 030 3-11-1; lane 10, O157-R1 -3- 2; lane 11, O157-C-1-2; lane 12, A1; lane 13, A2; lane 14, A3; ... P010726-21; lane 3, P010726-22; lane 4, P010726- 23; lane 5, P010726-24; lane 6, P010726-25; lane 7, P010726-26; lane 8, E010206- 13- 2; lane 9, J01 030 3-11-1; lane 10, O157-R1 -3- 2; lane 11, O157-C-1-2;...
  • 13
  • 456
  • 0
Báo cáo khoa học:

Báo cáo khoa học: " Isolation and identification of a canine coronavirus strain from giant pandas (Ailuropoda melanoleuca)" ppt

Báo cáo khoa học

... characterization of a new Australian isolate J Clin Microbiol 2001, 39 , 1 036 -1041 Nermut MV Negative staining of viruses J Microsc 1972, 96, 35 1 -36 2 Roche RR, Alvarez M, Guzman MG, Morier L, Kouri G Comparison ... with conventional tissue culture method for isolation of dengue virus in C6 /36 -HT cells J Clin Microbiol 2000, 38 , 35 08 -35 10 Tang G, Tang X, Gan D A method of preparing the section of eye tissue ... coronavirus (CCV) K378 (X77047) The asterisk indicates conserved amino acids between GPV and CCV K378; the dot indicates synonymous mutations of amino acids between GPV and CCV K378; the blanks...
  • 3
  • 308
  • 0
Báo cáo y học:

Báo cáo y học: "Isolation and identification of bioactive compounds in Andrographis paniculata (Chuanxinlian)" potx

Báo cáo khoa học

... 5.78 3. 97 4.67 4.52 ergosterol peroxide 4.7 4.71 5 .32 4.55 4.67 β-sitosterol and stigmasterol 5.2 5 .34 5.97 4.88 4.55 Steroids Flavonoids 5-hydroxy-7,8-dimethoxyflavone 6.1 4 .33 5 .34 3. 63 5.11 ... [28 ,31 -33 ] expression of the pi class of glutathione Stransferase Anti-cancer proliferation of HL-60 cells, the JAK-STAT3 pathway [44, 63] [99] CCl4, tBHP-induced hepatotoxicity (i.p 100 mg/kg, 3d) ... mg/kg, 3d) [91] caspase dependent Bid cleavage, caspase 3, activity, TRAIL-induced apoptosis, cell cycle arrest [48,52, 53, 63] tumor suppressor p 53 expression, MAPKs (p38 kinase, JNK, ERK1/2) signaling...
  • 15
  • 311
  • 1
Isolation and identification of components from ixeris sonchifolia hance as potential anti stroke agents

Isolation and identification of components from ixeris sonchifolia hance as potential anti stroke agents

Cao đẳng - Đại học

... correlations of compound 3. 3.1 .3 Chemical structures of compound and 10 68 3. 3.1.4 Chemical structures of compounds 11 to 13 71 3. 3.1.5 Chemical structures of compounds 14 to 18 71 3. 3.2.1 Representative ... preparations 60 3. 1.2 .3 Calibration graphs 60 3. 1.2.4 Quality control 60 3. 3 Results 61 3. 3.1 Characterization of purified compounds isolated from Ixeris sonchifolia Hance 61 3. 3.2 Quantitative ... Epidemiology 1.1 .3 Progress in stroke treatment 10 1.1 .3. 1 Thrombolysis 10 1.1 .3. 2 Anticoagulants and antiplatelet drugs 11 1.1 .3. 3 Neuroprotectants 13 1.1 .3. 4 Combination therapy 21 1.1 .3. 5 Traditional...
  • 188
  • 422
  • 0
isolation, selection and identification bacillus subtilis from muddreg of beer

isolation, selection and identification bacillus subtilis from muddreg of beer

Tổng hợp

... 14 iii 3. 1.4.Catalase .15 3. 1.5.Amylase .16 3. 1.6.Protease .16 3. 1.7.Reaction with Methyl Red 17 3. 1.8.Spore stained .17 3. 3.PCR product .18 3. 4.Sequencing ... 2. 83 0.74 12 BN3 long + + 1. 83 0.68 13 BN4 short + - 1. 43 0.71 14 BN5 short + + 1. 43 0.72 15 BN6 short + - 1.6 0.68 16 BN7 long + + 2.7 0.74 17 BN8 short + - 1. 43 0.68 18 BN9 long + + 2. 83 0.71 ... and Discussion 10 3. 1.Bacteria Isolation and characteristic 10 3. 1.1.Bacteria Isolation 10 3. 1.2.Characteristic (size of cell, Gram, movement) 12 3. 1 .3. Chemical reaction to test...
  • 29
  • 299
  • 0
Purification and cloning of PCR products

Purification and cloning of PCR products

Môi trường

... EcoRI 3 -dA overhang Taq DNA polymerase Added restriction site (blunt end) Type of end 5’CACC-target sequence 3 3 GTGG-target sequence 5’ 5’AATTCNNNN 3 AAAA3’GNNNN 5’ 5’GATCCNNNN 3 AAAA3’GNNNN ... denaturation; 136 PCR ● ● 94°C, 30 s3; 55°C4, 30 s3; 72°C, 30 s3 for 25 30 cycles; 72°C, Analyze a µl aliquot on an agarose gel 10 Meanwhile place the inoculated cultures in an orbital incubator at 37 °C ... AAAA5’GNNNN 3 3 ACGTCNNNN 5’ 5’NNNNNN 3 3 NNNNNN 5’ 5’ANNNNNN 3 3 ANNNNNN 5’ Sequence of end ’ Strand invasion of the added sequence by the complementary vector tail and TOPO ligation The other end...
  • 26
  • 555
  • 0
Tài liệu Báo cáo khoa học: Structure and function of active chromatin and DNase I hypersensitive sites pdf

Tài liệu Báo cáo khoa học: Structure and function of active chromatin and DNase I hypersensitive sites pdf

Báo cáo khoa học

... Journal compilation ª 2011 FEBS 2205 Active chromatin and DNase I hypersensitive sites 125 126 127 128 129 130 131 132 133 134 135 136 137 2206 P N Cockerill et al (2008) Combinatorial patterns ... histone variant H3 .3 EMBO J 28, 36 35 36 45 109 Kraus WL & Lis JT (20 03) PARP goes transcription Cell 1 13, 677–6 83 110 Kim MY, Mauro S, Gevry N, Lis JT & Kraus WL (2004) NAD+-dependent modulation ... factors HNF3 (FoxA) and GATA-4 Mol Cell 9, 279–289 237 Hatta M & Cirillo LA (2007) Chromatin opening and stable perturbation of core histone :DNA contacts by FoxO1 J Biol Chem 282, 35 5 83 35 5 93 238 Adams...
  • 29
  • 743
  • 0
Tài liệu Báo cáo Y học: Expression and characterization of active site mutants of hevamine, a chitinase from the rubber tree Hevea brasiliensis docx

Tài liệu Báo cáo Y học: Expression and characterization of active site mutants of hevamine, a chitinase from the rubber tree Hevea brasiliensis docx

Báo cáo khoa học

... 82.51 28.8–1.92 1.95–1.92 138 441 19106 98.5 (97 .3) 12.9 (5.5) 8.8 (24.5) 2079 57 – 1 43 17.5 21.6 0.005 1.5 23. 4 22.0 20.2 1 .3 23. 2 16.5 14.9 1.4 23. 1 16.5 13. 7 1 .3 23. 2 19.9 18.6 a Values in parentheses ... luteus cells (Sigma) were suspended in 10 mM Na-acetate buffer pH 5.0, to an OD600 of 0.7 Next, 3. 3– 33 pmol hevamine was mixed with mL M luteus suspension, depending on the activity of the hevamine ... hevamine and the Tyr183Phe and Asp125Asn mutants Km (lM) kcat (s)1) Hevamine Rec hevamine Asp125Asn Tyr183Phe 14 .3 16 .3 27.6 19.9 0.77 0.61 0.278 0.116 ± ± ± ± 2 .3 0.7 2 .3 2.4 ± ± ± ± kcat/Km...
  • 9
  • 616
  • 0
Báo cáo

Báo cáo " Analysis and identification of multi-variate random pressure fields using covariance and spectral proper transformations " pdf

Báo cáo khoa học

... 6m/s 77.46 13. 25 4. 23 2.86 1 .32 9m/s 75 .36 14.41 4.62 3. 17 1.45 B/D=1 with S.P 3m/s 6m/s 9m/s 65.29 62.79 63. 30 20.97 22.61 22.08 6.14 6.29 6.10 4.04 4 .32 4.41 1.99 2.28 2.45 3m/s 43. 77 22.02 ... Mode 3m/s 86.04 8.08 3. 28 1.40 0.64 B/D=1 6m/s 85.84 8.08 3. 20 1.62 0.72 9m/s 83. 02 9.92 3. 68 1.94 0.81 B/D=1 with S.P 3m/s 6m/s 9m/s 81 .30 77.48 77.88 10.15 12 .36 11.98 4.44 5.14 5.00 2.05 2. 63 ... fluctuating pressure 0.4 0 .35 0 .3 0 .3 0.25 0.45 0.4 0 .35 0.5 0.25 I u=11.46% Iw=11. 23% I u=10.54% Iw=9.28% I u=9.52% Iw=6.65% 0.4 C p,rm s C p,rm s 0 .3 0.25 0.2 Cp ,rms 0 .35 0.2 0.2 0.15 0.15 0.15...
  • 14
  • 390
  • 0
Báo cáo sinh học:

Báo cáo sinh học: "Pox proteomics: mass spectrometry analysis and identification of Vaccinia virion proteins" pot

Điện - Điện tử

... http://www.virologyj.com/content /3/ 1/10 1522.69 [MH]+ Virology Journal 2006, 3: 10 69.0 37 6.6 3 28 23 15 7 .3 2 .3 74 63 517 4 03 28 17 HTFNLYDDND I R 1522.64 730 .34 y++ -NH3 747 .33 y66 6 13. 35 b5 + 517 .31 y4+ * 9.1 3. 1 0 .3 3 .3 ... VP37, phoshoplipase D IMV membrane protein unknown Major core protein Glutaredoxin [17] [18] [19] [20] [21] [22] [ 23] [ 23] [24] [25, 26] [27, 28] [29] [30 ] [31 ] [32 ] [33 ] [34 ] [35 ] [36 ] [37 ] [38 ] ... 517 .31 y4+ * 9.1 3. 1 0 .3 3 .3 23 36 50 61 2727.4 632 .33 y5+ 1441.8 500 .31 b4+ * 36 3.18 b3+ + 4 03. 28 y3 ** 239 .14 b2+ Relative Abundance * 175.15 y1+ * 100 50 288. 23 y2+ 100 684.2 * m/z 991.8 1299.4...
  • 16
  • 331
  • 0
báo cáo hóa học:

báo cáo hóa học:"Pox proteomics: mass spectrometry analysis and identification of Vaccinia virion proteins" docx

Hóa học - Dầu khí

... http://www.virologyj.com/content /3/ 1/10 1522.69 [MH]+ Virology Journal 2006, 3: 10 69.0 37 6.6 3 28 23 15 7 .3 2 .3 74 63 517 4 03 28 17 HTFNLYDDND I R 1522.64 730 .34 y++ -NH3 747 .33 y66 6 13. 35 b5 + 517 .31 y4+ * 9.1 3. 1 0 .3 3 .3 ... VP37, phoshoplipase D IMV membrane protein unknown Major core protein Glutaredoxin [17] [18] [19] [20] [21] [22] [ 23] [ 23] [24] [25, 26] [27, 28] [29] [30 ] [31 ] [32 ] [33 ] [34 ] [35 ] [36 ] [37 ] [38 ] ... 517 .31 y4+ * 9.1 3. 1 0 .3 3 .3 23 36 50 61 2727.4 632 .33 y5+ 1441.8 500 .31 b4+ * 36 3.18 b3+ + 4 03. 28 y3 ** 239 .14 b2+ Relative Abundance * 175.15 y1+ * 100 50 288. 23 y2+ 100 684.2 * m/z 991.8 1299.4...
  • 16
  • 455
  • 0
báo cáo hóa học:

báo cáo hóa học:" Characterization of the tumor marker muc16 (ca125) expressed by murine ovarian tumor cell lines and identification of a panel of cross-reactive monoclonal antibodies" docx

Hóa học - Dầu khí

... 25 30 35 40 45 50 55 60 65 40 30 b 20 10 l1 l2 l3 l4 l5 o o Po o Po o Po o Po o P 50 Mean Fluorescence Intensity Fraction Number (1 mL/tube) 618F CAOV -3 SKOV -3 OVCAR -3 VK-8 40 30 20 10 CAOV -3 ... both 618F and 653F specifically bind to the OVCAR -3 but not to the MUC16neg SKOV -3 or CAOV -3 cells (Fig 4B) The binding of 618F, 653F and VK-8 to the OVCAR -3, SKOV -3, and CAOV -3 cells was comparable ... MUC16 was conducted using the 618F (3) and the VK-8 (4) antibodies (B) MUC16 expression on OVCAR -3, CAOV -3, and SKOV -3 was determined by flow cytometry using 653F, 618F, or VK-8 as the primary antibodies...
  • 7
  • 430
  • 0
Báo cáo hóa học:

Báo cáo hóa học: " Purification and characterization of novel fibrinolytic proteases as potential antithrombotic agents from earthworm Perionyx excavatus" pot

Hóa học - Dầu khí

... cloning and characterization of cDNA encoding fibrinolytic enzyme -3 from earthworm Eisenia foetida Acta Biochim Biophys Sin (Shanghai) 36 (4) :30 3–8 doi:10.10 93/ abbs /36 .4 .30 3 Einhaupl KM, Villringer A, ... 100 76 98 81 100 93 0 73 FI 602 1.8 0 EDTA 100 100 100 100 100 100 FII 44 0 Chymostatin 0.1 19 100 76 100 19 90 FIII-1 39 3 0.1 Pepstatin 100 58 60 86 83 75 FIII-2 7 83 0.4 FIII -3 831 1.2 0 FIV 296 ... large peak of 34 kDa protein (marked as FIV*) showed proteolytic activity (Figure 3A) FIII -3 was also separated into two peaks, namely FIII-3a and FIII-3b, with a MW of 33 and 31 kDa, respectively,...
  • 11
  • 375
  • 0
Báo cáo khoa học:

Báo cáo khoa học: "Purification and characterization of two larval glycoproteins from the cattle tick, Boophilus annulatus" doc

Báo cáo khoa học

... tnempiuqe dna serudecorp gniraer yrotarobaL AJ riaH ,DC kcirtaP 71 35 1-541 , 23 ,0991 aigolotissaraP lortnoc rof seigetarts dna skcit tsoh-itlum dna elgnis ni ecnatsiser ediciracA J naloN 61 633 -5 23 ,02 ... 32 611 -30 1 ,33 ,1002 dorP htlaeH minA porT mucilotana mucilotana ammolayH fo negitna lahpmyn aDk 93 deifirup a fo ycaciffe evitcetorp -onummI G saD ,HM nahK ,S hsohG ,KJ amrahS 22 641- 531 ,33 ... noitaziretcarahc dna noitacifiruP .38 -57 ,21 ,0991 lonummI etisaraP sulporcim sulihpooB htiw noitatsefni tsniaga elttac drofereH tcetorp snegitna enarbmem lavraL PJ kceebedpO ,MYJ gnoW 03 1 531 -6 431 ,34 1 ,9891...
  • 6
  • 267
  • 0
Báo cáo y học:

Báo cáo y học: "Genome-wide prioritization of disease genes and identification of disease-disease associations from an integrated human functional linkage network" pps

Báo cáo khoa học

... genome-wide linkage stud- 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 Volume 10, Issue 9, Article R91 Linghu et al R91.16 ies Nucleic Acids Res 2008, 36 :e26 Guan Y, Myers CL, Lu ... Chem 2005, 280: 230 24- 230 31 Tontonoz P, Mangelsdorf DJ: Liver X receptor signaling pathways in cardiovascular disease Mol Endocrinol 20 03, 17:985-9 93 Li AC, Glass CK: PPAR- and LXR-dependent pathways ... Huntington's disease Mol Cell 2004, 15:8 53- 865 Genome Biology 2009, 10:R91 http://genomebiology.com/2009/10/9/R91 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 Genome Biology 2009, Calvano...
  • 17
  • 415
  • 0
báo cáo khoa học:

báo cáo khoa học: " Screening and Identification of a Renal Carcinoma Specific Peptide from a Phage Display Peptide Library" pot

Báo cáo khoa học

... TXA: txabs9988@1 63. com ZJT: brianzg86@1 63. com WWW:Wangww2000@yahoo.com ZL: Liang2046@1 63. com ZLY: bytx1974@1 63. com ZJQ: jiquanzhao2010@1 63. com DCH:dch 031 0@1 63. com QSP: Urology@vip.1 63. com ZYY: yzhang@wfubmc.edu ... http://www.jeccr.com/imedia/946895945 630 233 8/supp1.pdf Additional file 2: Ethical approval-Pro.Tu 2.pdf, 741K http://www.jeccr.com/imedia/465 039 08 630 237 03/ supp2.pdf Additional file 3: Ethical approval-Pro.Tu.doc, ... for metastatic renal-cell cancer N Engl J Med, 2001, 34 5( 23) :1655 –1659 Cohen HT, McGovern FJ Renal-cell carcinoma N Engl J Med, 2005, 35 3( 23) :2477-2490 Tunuguntla HS, Jorda M Diagnostic and prognostic...
  • 28
  • 266
  • 0
Báo cáo y học:

Báo cáo y học: "Production, purification and characterization of polyclonal antibody against the truncated gK of the duck enteritis virus" pps

Báo cáo khoa học

... States, 19671995 J Wild Dis 2001, 37 :34 7 -35 7 Proctor SJ: Pathogenesis of duck plague in the bursa of Fabricius, thymus, and spleen Am J Vet Res 1976, 37 :427- 431 Levine PP, Fabricant J: A hitherto-undescribed ... characterization of the DNA of Anatid herpesvirus Intervirology 19 93, 36 :99-112 Zhang et al Virology Journal 2010, 7:241 http://www.virologyj.com/content/7/1/241 10 11 12 13 14 Page of Sandhu , ... polyclonal antibody J Virol Methods 2010, 1 63: 465-469 Reed LJ, Muench H: A simple method of estimating fifty per cent endpoints Am J Hyg 1 938 , 27:4 93- 497 Xie Wei, Cheng Anchun, Wang Mingshu, Chang...
  • 7
  • 270
  • 0

Xem thêm