... characterization of a new Australian isolate J Clin Microbiol 2001, 39 , 1 036 -1041 Nermut MV Negative staining of viruses J Microsc 1972, 96, 35 1 -36 2 Roche RR, Alvarez M, Guzman MG, Morier L, Kouri G Comparison ... with conventional tissue culture method for isolation of dengue virus in C6 /36 -HT cells J Clin Microbiol 2000, 38 , 35 08 -35 10 Tang G, Tang X, Gan D A method of preparing the section of eye tissue ... coronavirus (CCV) K378 (X77047) The asterisk indicates conserved amino acids between GPV and CCV K378; the dot indicates synonymous mutations of amino acids between GPV and CCV K378; the blanks...
... EcoRI 3 -dA overhang Taq DNA polymerase Added restriction site (blunt end) Type of end 5’CACC-target sequence 33 GTGG-target sequence 5’ 5’AATTCNNNN 3 AAAA3’GNNNN 5’ 5’GATCCNNNN 3 AAAA3’GNNNN ... denaturation; 136 PCR ● ● 94°C, 30 s3; 55°C4, 30 s3; 72°C, 30 s3 for 25 30 cycles; 72°C, Analyze a µl aliquot on an agarose gel 10 Meanwhile place the inoculated cultures in an orbital incubator at 37 °C ... AAAA5’GNNNN 33 ACGTCNNNN 5’ 5’NNNNNN 33 NNNNNN 5’ 5’ANNNNNN 33 ANNNNNN 5’ Sequence of end ’ Strand invasion of the added sequence by the complementary vector tail and TOPO ligation The other end...
... 25 30 35 40 45 50 55 60 65 40 30 b 20 10 l1 l2 l3 l4 l5 o o Po o Po o Po o Po o P 50 Mean Fluorescence Intensity Fraction Number (1 mL/tube) 618F CAOV -3 SKOV -3 OVCAR -3 VK-8 40 30 20 10 CAOV -3 ... both 618F and 653F specifically bind to the OVCAR -3 but not to the MUC16neg SKOV -3 or CAOV -3 cells (Fig 4B) The binding of 618F, 653F and VK-8 to the OVCAR -3, SKOV -3, and CAOV -3 cells was comparable ... MUC16 was conducted using the 618F (3) and the VK-8 (4) antibodies (B) MUC16 expression on OVCAR -3, CAOV -3, and SKOV -3 was determined by flow cytometry using 653F, 618F, or VK-8 as the primary antibodies...
... States, 19671995 J Wild Dis 2001, 37 :34 7 -35 7 Proctor SJ: Pathogenesis of duck plague in the bursa of Fabricius, thymus, and spleen Am J Vet Res 1976, 37 :427- 431 Levine PP, Fabricant J: A hitherto-undescribed ... characterization of the DNA of Anatid herpesvirus Intervirology 19 93, 36 :99-112 Zhang et al Virology Journal 2010, 7:241 http://www.virologyj.com/content/7/1/241 10 11 12 13 14 Page of Sandhu , ... polyclonal antibody J Virol Methods 2010, 1 63: 465-469 Reed LJ, Muench H: A simple method of estimating fifty per cent endpoints Am J Hyg 1 938 , 27:4 93- 497 Xie Wei, Cheng Anchun, Wang Mingshu, Chang...