kernel mutants of corn
Tài liệu Báo cáo khoa học: Enzymatic properties of wild-type and active site mutants of chitinase A from Vibrio carchariae, as revealed by HPLC-MS pptx
... investigated Of three newly generated chitinase A variants, the hydrolytic activity of E315M and E315Q mutants was entirely abolished Apparently, structural modification of the carboxylate side-chain of ... thank Prof Steve C Fry, Institute of Cell and Molecular Biology, University of Edinburgh, Edinburgh, UK, and Dr Albert Schulte, Ruhr University of Bochum, Germany, for critical reading of the ... means of a pNP calibration curve in the case of the colorimetric assay, or by using a (GlcNAc)2 calibration curve in the case of quantitative HPLC-MS If pNP is used for monitoring the hydrolysis of...
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Tài liệu Báo cáo khoa học: The calcium-induced switch in the troponin complex probed by fluorescent mutants of troponin I doc
... reconstitution of troponin complexes The ability of each mutant TnI to bind TnC was assessed by urea/ PAGE in the presence of (A) 0.5 mM EDTA and (B) 0.5 mM Ca2+ In the absence of Ca2+ only the band of free ... different sets of data: one for probes in the inhibitory region of TnI, another for the probe at position 121 of TnI, and a third for the probe in the N-domain of TnC Fig Calcium titration of the fluorescent ... set of four TnC mutants combined with TnIF100HW or TnIM121HW There is an aspartic acid involved in metal ion coordination in the first position of all EF-hands of TnC This allowed each one of the...
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Tài liệu Báo cáo khoa học: The kinetic properties of various R258 mutants of deacetoxycephalosporin C synthase doc
... of penicillin G or ampicillin at a single defined concentration of the 2-oxoacid cosubstrate (2 mM) Results and discussion Purification and characterization of mutants Wild-type DAOCS and all mutants ... The effect of mutations appears to be similar regardless of whether the prime substrate was penicillin G or ampicillin Utilization of 2-oxoglutarate by the R258 mutants The level of 2-OG conversion ... combination of anion-exchange and gel filtration chromatographies The DNA sequences of the R258 mutants and the ESI-MS analyses showed that only Ó FEBS 2003 Co-substrate selectivity of DAOCS (Eur...
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Tài liệu Báo cáo Y học: Expression and characterization of active site mutants of hevamine, a chitinase from the rubber tree Hevea brasiliensis docx
... Total number of observations Number of unique reflections Completeness (%)
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Báo cáo khoa học: Mutants of Saccharomyces cerevisiae deficient in acyl-CoA synthetases secrete fatty acids due to interrupted fatty acid recycling docx
... role of acyl-CoA synthetases in regulating particular pools of fatty acids, we used mutants of S cerevisiae characterized by various combinations of deletions in the corresponding genes In mutants ... 2769 Fatty acid secretion in mutants of yeast M Scharnewski et al Fig TLC of lipid extracts of yeast strains fed with radiolabelled oleic acid Cells of wild-type and the mutants indicated were grown ... However, analysis of lipid extracts of the transformed cells demonstrated 18:2 to be a significant constituent of the pool of esterified fatty acids, indicating successful expression of the D12-desaturase...
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Báo cáo khoa học: Active site mutants of Drosophila melanogaster multisubstrate deoxyribonucleoside kinase pot
... specificity of the enzymes However, the single amino acid mutants of N28P and I29H or the combination of the two mutants with or without the F114Y showed complete or almost complete loss of activity ... site-directed mutagenesis of the residues N28, I29, F114 and Q81 in the Dm-dNK active site The aim of the study was in part to understand the determinants of the substrate specificity of the enzyme and ... gain further understanding of the catalytic mechanism of the enzyme and the properties determining the broad substrate specificity of the enzyme Although the maximal rate of purine and pyrimidine...
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Báo cáo khoa học: Effect of valine 106 on structure–function relation of cytosolic human thymidine kinase Kinetic properties and oligomerization pattern of nine substitution mutants of V106 ppt
... Furthermore, a prediction of the secondary structure of TK1 [30–32] places Val106 in the middle of an a-helix which aligns in CLUSTAL [33] with one of the interface helices (a-helix 4) of HSV1TK This may ... and V106T We then characterized the enzymatic properties of the mutant enzymes The apparent native sizes of the –ATP forms of V106WT and of the mutant recombinant enzymes (subunit size 24 kDa, ... were eluted essentially as V106WT: a substantial part of each of these enzymes was Fig Gel filtration of rLy-TK166)136 proteins About 100 lg of rLyTK166)136(Val106) (d) and rLy-TK166)136(Met106)...
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Báo cáo khoa học: Characterization of Met95 mutants of a heme-regulated phosphodiesterase from Escherichia coli ppt
... Fe(II)-CO (…) complexes of wild-type (A), Met95Ala (B), Met95Leu (C) and Met95His (D) mutants of isolated heme-bound PAS domain MCD spectra of the wild-type and Met95 mutants of the full-length enzyme ... from those of the Met95Ala and Met95Leu mutants Specifically, the Soret MCD intensities of the Fe(II)–CO complexes of the Met95Ala and Met95Leu mutants were 20–30% higher than those of the Fe(II)–CO ... denaturation of the Met95 mutants occurred occasionally at high temperatures during expression or as a consequence of auto-oxidation of the O2-bound Fe(II) complexes during purification, often resulting...
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Báo cáo khoa học: Transport of taurocholate by mutants of negatively charged amino acids, cysteines, and threonines of the rat liver sodium-dependent taurocholate cotransporting polypeptide Ntcp docx
... domains model), is involved in taurocholate transport of the human isoform [21] We therefore looked for the role of each of the eight cysteines of the rat Ntcp for taurocholate transport Finally, ... at the beginning of the C-terminal tail of Ntcp (Fig 7) 1122 D Zahner et al (Eur J Biochem 270) Ó FEBS 2003 Fig Sodium dependency of taurocholate uptake by Ntcp mutants Uptake of lM [3H]taurocholate ... [3H]taurocholate for 30 The amount of cRNA of transport-negative mutants was increased 14-fold; the standard amount of cRNA which was injected for comparison of transport by mutated vs wild-type...
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Báo cáo khoa học: Substrate specificity and excision kinetics of natural polymorphic variants and phosphomimetic mutants of human 8-oxoguanine-DNA glycosylase pot
... phosphorylation of OGG1 at sites of high phosphorylation probability could influence the ability of APEX1 to stimulate OGG1 To address this question, we investigated the activity of phosphomimetic mutants of ... Time course of excision of 8-oxoGua (d), FapyGua (s) and FapyAde ( ) by OGG1 (B) Time course of excision of 8-oxoGua (d), FapyGua (s) and FapyAde ( ) by Fpg Means and standard deviations of three ... all plots Means of two independent experiments are shown [P], concentration of the AP product of this variant in vivo and contribute to the pathogenesis of Alzheimer’s disease Of all variants...
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A Dissertation Presented to the Faculty of the Graduate School of Cornell University In Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy potx
... Professor Jeevak Parpia’s lab at Cornell University before heading off to Colorado in August 2008 to become an Assistant Professor of Mechanical Engineering at the University of Colorado at Boulder iii ... of valuable criticisms and suggestions I couldn’t incorporate all of their suggestions, so not fault them if you find parts of this thesis disagreeable or in need of revision A crucial part of ... meV A whole subfield of condensed matter physics grew out of the study of these frustrated little conducting islands For a more extensive review of quantum dots discussing all of these situations...
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Analysis and Control of Corn plex Nonlinear Processes pptx
... (lower row) kinetics t = 0.05 from one of them leads the system into the basin of attraction of the other one The basin of attraction of a fixed point is that part of phase space from which systems ... value Xopt is of the same order of magnitude as the width of the pulse front 1.5.2 Propagation of trigger waves i n the presence of noise We study in this subsection the effect of noise on pulses ... of large nonequilibriumsystems; problems of nonlinear pattern formation in chemistry; complex organization of intracellularprocesses and biochemicalnetworksof a living cell; various aspects of...
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Báo cáo hóa học: " Human adenovirus type 19 infection of corneal cells induces p38 MAPK-dependent interleukin-8 expression" potx
... the human cornea, HAdV-19 infection leads to infiltration of leukocytes, presumably in response to chemokines produced by infected corneal cells [28] In vitro, HAdV-19 infection of corneal cells ... protocol for use of corneas from deceased human donors was approved by the University of Oklahoma Institutional Review Board, and conformed to the tenets of the Declaration of Helsinki HAdV-19 ... regulation of IL-8 in corneal cells remains unknown The eye represents a major target of adenovirus infection, and the resultant inflammation, particularly in the HAdV19-infected cornea, can...
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Báo cáo hóa học: "Research Article Kernel Learning of Histogram of Local Gabor Phase Patterns for Face Recognition" pptx
... [22] and kernel Fisher discriminant analysis (KFDA) [23, 24], which are nonlinear extensions to PCA and FDA, respectively The selection of kernel function is one of open problems for the kernel- based ... mercer’s kernels are available, such as polynomial, Gaussian, RBF, and so on We also find that some special kernel functions, GW-chi [15] and HI -kernel [14], have been successfully used in the field of ... and so forth The performances of popular statistical or learning methods degrade abruptly, if the distribution of the testing samples is very different from that of the training set Eigenface...
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Science Report: "Determining the value of an exchange energy of corn as feed for chickens by direct methods" pps
... 80% energy of diets Most of our results which determined the ME values of corn varieties by the biological method on 11 varieties of corn (in of 11 varieties of corn, the ME value of them were ... one was of Silidim variety, and the lowest one was of Bioseed 9723 (10.90%) The water content or the moisture of corn is one of the important factors, which not only affects the quality of corn, ... (NRC, 1998) The GE values of corn, which were estimated by the indirect method of Ewan 1989 (NRC, 1998), were in the range of 4334 – 4485 kcal Thus, the GE values of corn (according to the direct...
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Báo cáo khoa học: "Surgical correction of corneal dermoid in a dog" doc
... nilyxotameh htiw deniats dna ,ylenituor dessecorp ,nilamrof deffub-etahpsohp lartuen %01 ot detcelloc saw 04× ,E&H ,)VB( lessev doolb ,)FH( elcillof riah ,)C( muinroc ,)EC( muilehtipe laenroc ;god ... deraeppasid saw arohpipe dna avitcnujnoc fo aimerepyh dna ,noitcerroc lacigrus eht ecnis shtnom 11 rof derrucer t’nsah diomred ehT )2 giF( racs eht deniamer dna dezilehtipe saw palf lavitcnujnoc fo ... giF secnerefeR won ot pu raeppa ton did ecnerrucer dna yllufsseccus devomer saw diomred ,yletanutroF ylevisavni erom dednetxe dna ezis ni desaercni saw noisel eht ,yltneuqesnoC secnatsmucric s’renwo...
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Báo cáo khoa hoc:"Mapping the Naked Neck (NA) and Polydactyly (PO) mutants of the chicken with microsatellite molecular markers" docx
... mutation Hx In the case of the NA locus, markers of chromosome were selected because CPPP was mapped on this chromosome Analysis of individual typings showed a linkage of 5.7 cM (lod score = 13) ... region of chromosome 3q These results contribute to connecting the former classical map to the molecular genetic map of the chicken, and open the way to the identification of the molecular nature of ... free of ALVE insertions [16] that was imported from Ottawa, Canada A total of 70 progeny was scored for the presence of ∗N A or ∗P O mutant sire alleles In order to overcome the difficulty of variable...
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báo cáo khoa học: "Effect of alcohol and competition levels on viability of eye colour mutants of Drosophila melanogaster" potx
... analysis of eye colour mutants carried out in cellar and vineyard , AJERA populations (N unpublished data), the number of eye colour mutants per female was 0.628 ± 0.040 (93 mutants of 148 wild ... Valencia (Spain) were used to establish isofemale strains Four of them were eye colour mutants : allele of sepia (se an allele of safranin (sf an allele of cardinal (cd and °) ; 77 ) ; 77m °) ?7 ... and 0.331 + 0.043 (40 mutants of 121 females analysed) in a vineyard km away from the cellar In order to examine the influence of alcohol on the viability of eye coulour mutants obtained in a...
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Involvement of p63 in the herpes simplex virus-1-induced demise of corneal cells Research ppt
... 99% of SIRC cells infected at an MOI of (Fig 2) MTT assays to evaluate the cytopathogenicity of HSV-1 revealed decreased viability at 48 hpi in 23 and 36% of SIRC cells infected at MOIs of and ... Journal of Biomedical Science 2010, 17:47 http://www.jbiomedsci.com/content/17/1/47 Page of Figure Serological identification of the p63 isoforms expressed in HSV-1-infected SIRC cells The levels of ... representative of three independent experiments decreased (the relative quantities of TAp63γ in cells infected at an MOI of 10 were 0.11 and 0.19) (Fig 6; lanes and 3) The expression of the TAp63γ isoform...
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Cytoskeleton regulators in the control of corneal fibrosis
... 33 3.2 Effect of various dosing regimes of topical application of TGF-β1 on the transformation of corneal keratocytes-to-myofibroblasts in the corneal fibrosis in vivo The effect of various dosing ... dosing regimes of topical application of TGF-β1 on the transformation of corneal keratocytes-to-myofibroblasts in the development of corneal fibrosis in vivo, as defined by the expression of α-SMA, ... involve this family of proteins Our clinical target is the early phase of corneal wounding, before an opaque corneal scar forms In response to corneal injury, the transformation of corneal stroma...
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