k l and s activity profiles in human lung cancer
Báo cáo sinh học: "Multiplex Zymography Captures Stage-specific Activity Profiles of Cathepsins K, L, and S in Human Breast, Lung, and Cervical Cancer" potx
... were expressed in insect cells, human cathepsin V was expressed in NSO cells, and human cathepsin L was isolated from liver Western blotting SDS-PAGE was performed, and protein was transferred ... Profiles of Cathepsins K, L, and S in Human Breast, Lung, and Cervical Cancer Binbin Chen and Manu O Platt* Abstract Background: Cathepsins K, L, and S are cysteine proteases upregulated in cancer and ... unique in that its activity was binary: off in normal tissue and on in cancerous tissue Cathepsin K in breast tissue had the lowest variability and consistently low baseline for cathepsin K activity...
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báo cáo khoa học: "Inhibition of angiogenesis- and inflammation-inducing factors in human colon cancer cells in vitro and in ovo by free and nanoparticle-encapsulated redox dye, DCPIP" pptx
... Barbatis C, Lea RW, Kalofoutis A, Korkolopoulou P: Analysis of interleukin (IL)-8 expression in human astrocytomas: associations with IL-6, cyclooxygenase-2, vascular endothelial growth factor, and ... days turning them at regular intervals to achieve nearly complete vasculogenesis and blood vessel development progressing mostly through angiogenesis On day 10, the eggs were candled and a small ... immunity plays a crucial role in angiogenesis, organogenesis, and the pathogenesis of various diseases including tumor development, growth and metastasis, atherosclerosis, sepsis, and rheumatoid...
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Báo cáo khoa học: Transport of L-arginine and nitric oxide formation in human platelets pdf
... control and each treatment at least at 5% level (P < 0.05) Results L- Arginine in ux in human platelets N-ethylmaleimide inhibits selectively system y+, leaving system y +L functionally intact ... replaced by choline salts In some experiments washed platelets were resuspended in pH 7.4 Hepes buffer containing lM prostaglandin E1 In these conditions the platelet L- arginine in ux was unchanged ... the assay of cGMP level, suggesting that the L- arginine transport systems y +L and y+ are both implicated in NO production Thus extracellular L- arginine may modulate intracellular NO synthesis by...
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Báo cáo hóa học: " Research Article Joint Wavelet Video Denoising and Motion Activity Detection in Multimodal Human Activity Analysis: Application to Video-Assisted " doc
... noise problems Based on the qualitative and quantitative analysis results, the proposed methodology is expected to be easily extendible to similar video surveillance tasks, as well as in demanding ... Dimoulas, G M Kalliris, G V Papanikolaou, and A Kalampakas, “Long-term signal detection, segmentation and summarization using wavelets and fractal dimension: a bioacoustics application in gastrointestinal-motility ... Hensley, D R Hillman, R D McEvoy, et al., “Guidelines for sleep studies in adults,” in The Australasian Sleep Association & Thoracic Society of Australia and New Zealand, pp 1–38, Sydney, Australia,...
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Báo cáo y học: " Profiles of cytokine and chemokine gene expression in human pulmonary epithelial cells induced by human and avian influenza viruses" doc
... nearby cells) manner Cytokines released following infection can be classified broadly into “early” and “late” cytokines In this study, the transcription levels of cytokines/chemokines were delineated ... growing stocks of influenza virus isolates MDCK cells were grown and maintained in Eagles Minimal Essential Media (MEM) containing 2% FBS, 100 U/ml penicillin and 100 μg/ml streptomycin (all from ... LL, et al: Proinflammatory cytokine responses induced by influenza A (H5N1) viruses in primary human alveolar and bronchial epithelial cells Respir Res 2005, 6:135 Julkunen I, Melen K, Nyqvist...
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3D Videocommunication Algorithms concepts and real time systems in human centred communication
... more and more established in daily use, especially in offices and in home environments Immersive systems will leave its experimental state and immersive portals will become ubiquitous in business ... various devices ranging from inflatable pressure pads in data gloves or body suits to force-feedback arms or exoskeleton systems Although there is an increasing interest in engineering truly multisensory ... Factors of 3D Displays by W.A IJsselsteijn, P.J.H Seuntiens and L. M.J Meesters The authors discuss several aspects of stereoscopic viewing The human factors aspect is essential for the development...
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Báo cáo khoa học: Characterization of rat cathepsin E and mutants with changed active-site residues and lacking propeptides and N-glycosylation, expressed in human embryonic kidney 293T cells ppt
... Langerhans cells, and interdigitating reticulum cells [4] However, fundamental information on the biosynthesis, processing, and intracellular trafficking of this protein as well as its physiological significance ... cathepsin E expressed in Chinese hamster ovary cells is found in various subcellular compartments such as the vacuolar endolysosomal system, the ER, and the cytosol [37] The present results thus indicate ... oligosaccharide chains of rat cathepsin E in the transfected cells and then assessed the role of N-glycosylation in its folding, processing, maturation, and subcellular trafficking HEK-293T cells...
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Báo cáo khoa học: "Characterizing and Recognizing Spoken Corrections in Human-Computer Dialogue" pot
... 737 ening played a role However, in only one of these studies was input "conversational", the other was a form-filling application, and neither involved spoken system responses, relying instead ... the same words as the original For example, SYSTEM SAID: Please say mail, calendar, weather, stock quotes or start over to begin again USER SAID: MAIL SYSTEM HEARD: MAIL CODE: OK SYSTEM SAID: Switching ... example of pause ,° Using the features described above, we performed some initial simple statistical analyses to identify those features which would be most useful in distinguishing original inputs...
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Báo cáo khoa học: Novel ATP-binding and autophosphorylation activity associated withArabidopsisand human cryptochrome-1 pptx
... phosphoamino acid analysis [18] As shown in Fig 3B, only labeled phospho-serine was detected, and the nonradiolabeled standards (phospho-serine, -threonine, and -tyrosine) were clearly visible as well ... Cryptochromes have been identified as substrates for protein kinases in several systems [20,21] and have no homology to known protein kinases themselves Therefore, to eliminate the formal possibility ... gels The left panel represents the Coomassie stained protein samples, in the right panel the autoradiogram of these samples is shown protein expressed in Sf21 cell extracts is purified on Co2+ instead...
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Báo cáo Y học: Monitoring the structural consequences of Phe12 fi D-Phe and Leu15 fi Aib substitution in human/rat corticotropin releasing hormone Implications for design of CRH antagonists pdf
... helical character and a small bend along the principal molecular axis As far as the ill-defined N terminus is concerned, all data (chemical shift index, 3JHNHa, and u vs w plots, Fig S2 ) imply ... clinical applications in psychiatric disorders MATERIALS AND METHODS Sample preparation The CRH analogue was synthesized using Fmoc/tBu chemistry by solid-phase peptide synthesis and was purified and ... 6–7 Hz and were not used in structural calculations [27] Slowly exchanging amides were identified using the lyophilized original sample in D2O/TFE-d3 solution, through two-dimensional NOESY spectra...
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Báo cáo khoa học: Expression of heme oxygenase-1 is repressed by interferon-c and induced by hypoxia in human retinal pigment epithelial cells pot
... oxidative stress in two human RPE cell lines, D407 and ARPE-19 [21,25], indicating that these RPE cells possess the defense system involving HO-1 These RPE cell lines therefore provide a good system to ... Basic) was used as a control Expression of reporter genes and pRL-TK was determined with the Dual-LuciferaseTM Reporter Assay System (Promega) Results Western bolt analysis D407 human RPE cells ... repressor of the HO-1 gene in mice [47] and in cultured human lung cancer cells [27] The expression levels of HO-1 and Bach1 mRNAs are inversely regulated by interferon-c in both RPE cell lines In...
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Báo cáo khoa học: cGMP and glutathione-conjugate transport in human erythrocytes The roles of the multidrug resistance-associated proteins, MRP1, MRP4 and MRP5 pot
... Cell lines COR -L2 3/R and MOR/R are MRP1-overexpressing, multidrug-resistant, human large-cell lung tumour lines produced by doxorubicin selection [22,23] All cells were cultured on plastic in ... 1.1 osmolality but assayed in NaCl/Pi with an osmolality 10–100-fold higher To check these solutions as possible in uencing factors, cGMP uptake in the current study was also measured using solutions ... Doxorubicin-resistant lung adenocarcinoma cell line MOR/R was used as a positive control; doxorubicin-sensitive human large-cell lung tumour cell line COR -L2 3/P was used as a negative control high-affinity...
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Báo cáo khoa học: DYRK1A phosphorylates caspase 9 at an inhibitory site and is potently inhibited in human cells by harmine pptx
... kinase in cells Previous studies have shown that caspase is phosphorylated on a single major site, Thr125, catalysed by the proline-directed kinases ERK1 ⁄ MAPKs and CDK1–cyclin B1 in response ... diseases, including malarial infection and Parkinson s disease [42] Harmine and related b-carbolines have cytotoxic activity towards human tumour cell lines in culture [43], suggesting a possible ... (T125) H.sapiens M.musculus R.norvegicus Casp9 Actin Fig Small-molecule inhibitors suggest DYRK1A as a potential kinase that phosphorylates caspase on Thr125 (A) U2.C9–C287A cells stably expressing...
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Báo cáo khoa học: Downregulation of protease-activated receptor-1 in human lung fibroblasts is specifically mediated by the prostaglandin E2 receptor EP2 through cAMP elevation and protein kinase A pot
... Harley RA, Silver RM & Ludwicka-Bradley A (2005) Distinct PKC isoforms mediate cell survival and DNA synthesis in thrombin-induced myofibroblasts Am J Physiol Lung Cell Mol Physiol 288, L1 90 L2 01 ... forskolin for 15 Cells were washed in NaCl ⁄ Tris and lysed using the provided lysis ⁄ wash buffer containing a protease inhibitor cocktail (Roche Molecular Biochemicals, Mannheim, Germany) Cell ... expression of PAR-1 in other cell lines expressing this receptor We used the human astrocytoma cell line 1321N1 and the human alveolar epithelial cell line A549 In 1321N1 cells, mithramycin A reduced...
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Báo cáo khoa học: "INTEGRATING MULTIPLE KNOWLEDGE SOURCES FOR DETECTION AND CORRECTION OF REPAIRS IN HUMAN-COMPUTER DIALOG*" potx
... strings, the less likely the phrase was a repair SIMPLE PATTERN These numbers show that although pattern matching is useful in identifying possible repairs, it is less successful at making appropriate ... preceding M1; for false positives, this increase in F0 was rarely observed Table shows the results of combining the acoustic constraints just described As can be seen, such features in combination ... helpful in detecting repairs is acoustics In this section we describe firsthow prosodic information can help in distinguishing repairs from false positives for patterns involving matched words...
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Báo cáo hóa học: " Nutraceutical augmentation of circulating endothelial progenitor cells and hematopoietic stem cells in human subjects" potx
... cell (EPC) levels subsequent to administration (Mikirova et al Journal of Translational Medicine in press) In the current study we sought to assess kinetics of EPC and stem cell mobilization in ... Assessment of cells bearing this phenotype was performed at similar timepoints to CD34/C133 expression pre- and postStem-Kine administration Significant increases of circulating cells expressing ... research is required to assess physiological effects in humans In conclusion, the current study suggests feasibility of significant mobilization of cells expressing hematopoietic stem cell and...
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báo cáo hóa học:" Effect of borax on immune cell proliferation and sister chromatid exchange in human chromosomes Malinee Pongsavee" docx
... with loss of mammary myoepithelial cells in tissue culture provides a potential mechanism for increasing invasive mammary carcinoma [5] and induced colonic neoplasia in animal models [6] Borax is ... materials and humoral immune cell still need to be investigated for food additive carcinogenesis The defect in genetic material and immune cell development involves in human carcinogenesis In this study, ... of living cells A solubilization solution is added to dissolve the insoluble purple formazan product into a colored solution The absorbance of this colored solution can be quantified by measuring...
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Báo cáo hóa học: " Subcellular forms and biochemical events triggered in human cells by HCV polyprotein expression from a viral vector" doc
... tubular structures (TS), electron dense structures in membranous webs (EDS) Bar: 500 nm h p.i for EM analysis In this cell line, similarly as in HeLa cells, the inducible expression of HCV proteins ... immunogold labelling on ultrathin sections Due to the fixation and embedding procedures used in immunostaining, the cell structures are less visible than by embedding in an epoxy resin While in cells ... cultured cells [7,9] Although these systems produced infectious HCV, the virus yields are low, not all cells become infected and the virus growth is only observed in certain cell lines In this...
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báo cáo hóa học:" Cytokine Profiles in Human Immunodeficiency Virus-Infected Children Treated With Highly Active " pdf
... disclosed no relevant financial relationships Susan S. S Chiu, MD, has disclosed no relevant financial relationships Wilfred H .S Wong, MMedSci, has disclosed no relevant financial relationships ... Wilina W .L Lim, MD, has disclosed no relevant financial relationships Yu -lung Lau, MD, has disclosed no relevant financial relationships Acknowledgements We wish to thank all of the patients and ... in patients, within 24 months in patients, and after 16 and 19 months in patients ELISPOT Assay Numbers of cytokine-secreting cells in unstimulated cultures or cultures stimulated with T-cell...
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