k atpase inhibition on calcium regulation in cardiomyocytes

báo cáo hóa học:" The effect of oxygen tension on calcium homeostasis in bovine articular chondrocytes" pptx

Ngày tải lên : 20/06/2014, 04:20

... chick chondrocytes by maintenance under lowered oxygen tension Mech Ageing Develop 1988, 45:157-165 Sanchez JC, Wilkins RJ: Mechanisms involved in the increase in intracellular calcium following ... Wilkins RJ: Rapid effects of hypoxia on H+ homeostasis in articular chondrocytes Pflug Archiv 2009, 458:1085-1092 61 Browning JA, Wilkins RJ: The effect of intracellular alkalinisation on intracellular ... Hypoxia also induced depolarization of mitochondria, indicative of a reduction in electron flow through the mitochondrial electron transport chain, and hence ROS production Addition of antimycin A also...

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Báo cáo khoa học: Action of palytoxin on apical H+/K+-ATPase in rat colon pdf

Ngày tải lên : 17/03/2014, 11:20

... membrane of colon epithelial cells Ouabain inhibition of the colonic H+ /K+ -ATPase has been a matter of controversy in many investigations Early experiments involving ATPase measurements on apical ... cations fail to be conducted Taking into consideration the relatively high homology of the Na+ /K+ -ATPase and colonic H+ /K+ -ATPase with the gastric H+ /K + -ATPase, one might expect to obtain similar ... depolarization (Fig 2) The inhibition of palytoxin-induced currents by vanadate, a known blocker of apical H+ /K+ -ATPase [22], suggests that this enzyme is involved in the formation of the palytoxininduced...

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Financial distress prediction based on OR-CBR in the principleof k-nearest neighbors

Ngày tải lên : 23/12/2013, 17:17

... jxik À x 0k j > vk ð20Þ d k ðci ; c0 Þ ¼ jxik À x 0k j À pk ; vk À p k if pk < jxik À x 0k j vk ð21Þ In this deﬁnition, vk is veto threshold parameter of feature Fk such that the assertion of ci indifferent ... $ x 0k c0, $ xik ci, $ qk [0, 2], $ pk [0, 2], qk < pk If the condition, qk < jcik À c0kj pk is met, then what it means is that ci is weakly difference to c0 if feature Fk is taken into consideration, ... dis(ak,bk) = j norm(ak) À norm(bk)j, into consideration directly, the distance measure for the kth feature has the following properties • dis(ak,bk) = if and only if ak = bk • dis(ak,bk) = dis(bk,ak)...

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Báo cáo Y học: Irregular spiking in free calcium concentration in single, human platelets Regulation by modulation of the inositol trisphosphate receptors ppt

Ngày tải lên : 17/03/2014, 17:20

... Irregular spiking in [Ca2+]i in single platelets independently of InsP3 formation Fura-2-loaded platelets immobilized on ﬁbrinogen often exhibit ÔspontaneousÕ, spiking increases in [Ca2+]i, which ... blocked the thrombin-induced increase in InsP3 level at concentrations that also suppressed the thrombin-induced Ca2+ response Together these results indicate that both InsP3-generating (thrombin) ... lM) in the presence of mM EGTA Fig Confocal monitoring of ÔspontaneousÕ spiking in [Ca2+]i in spread platelets Fluorescence changes were monitored by confocal laser scanning microscopy in aspirin-treated,...

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Báo cáo khoa học: No evidence for a role in signal-transduction of Na+/K+-ATPase interaction with putative endogenous ouabain potx

Ngày tải lên : 23/03/2014, 17:21

... ouabain-sensitive Na+ /K+ -ATPase is knocked-out by incubation of the cells in micromolar concentrations of ouabain [16] Were signal-transduction of signiﬁcance in this situation, the phenomenon ... elicited by the interaction of a minor fraction of ouabainaltered Na+ /K+ -ATPase with neighbouring proteins (Src, growth factor receptors and Ras), i.e by protein–protein interaction In myocytes, ... obtain Ca2+-oscillations in a reasonable fraction of the rat kidney cells [8], though oscillations were seen in a few cells exposed to 10–100 nM ouabain for h As ouabain binding to Na+ /K+ -ATPase...

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Báo cáo Y học: High pressure-induced changes of biological membrane Study on the membrane-bound Na+/K+-ATPase as a model system pdf

Ngày tải lên : 24/03/2014, 00:21

... tritium incorporation into Na+ /K+ -ATPase are shown in Fig Tritium incor- Fig Eect of pressure on tritium incorporation into membrane-bound Na+ /K+ -ATPase Membrane-bound Na+ /K+ -ATPase was mixed ... emission maximum (kmax) and intensity of intrinsic ¯uorescence of Na+ /K+ -ATPase under increasing pressures are shown in Fig 2A The value for kmax was shifted from 341 to 345 nm with increasing ... reaction rate of the Na+ /K+ -ATPase with an increase in pressure can be rationalized based on a decrease in ¯uidity of the lipid bilayer with a concomitant decrease in protein conformational ¯exibility...

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Report on the Regulation of Reproductive Cell Donation in the European Union potx

Ngày tải lên : 28/03/2014, 16:20

... explicitly included in the law or government rules and is binding It is included in national or international organisation guidelines and is binding It is recommended by national or international organisations ... appearing in person at the institution for harvesting the substance containing the reproductive cells When donating the cells for a reproduction procedure, the donor’s declaration shall contain ... donations of reproductive cells are made repeatedly, on an ongoing basis The ongoing nature of the donations does not exempt the donor from providing information on any known illnesses.” IT Donor...

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Báo cáo khoa học: Impact of cyclic hypoxia on HIF-1a regulation in endothelial cells – new insights for anti-tumor treatments doc

Ngày tải lên : 30/03/2014, 02:20

... signaling enzymes, including Akt, ERK and eNOS Using pharmacological inhibitors, we identiﬁed the key role for the reoxygenation-driven PI 3K ⁄ Akt pathway in stabilization of HIF-1a during consecutive ... PI 3K ⁄ Akt activation occurring during the reoxygenation phases accounts for the observed CyHdriven HIF-1a stabilization, a phenomenon further exacerbated by the increase in O2 consumption in ... Brurberg KG, Skogmo HK, Graff BA, Olsen DR & Rofstad EK (2005) Fluctuations in pO2 in poorly and well-oxygenated spontaneous canine tumors before and during fractionated radiation therapy Radiother Oncol...

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Báo cáo khoa học: Mitochondrial calcium signalling in cell death Delivered on 1 July 2004 at the 29th FEBS Congress in Warsaw pptx

Ngày tải lên : 30/03/2014, 20:20

... cells inhibition of mitochondrial Ca2+ uptake resulted in reduction of ER Ca2+ release [48], indicating that in this case mitochondria play a major role in preventing the Ca2+-depended inhibition ... Mitochondrial calcium signalling in cell death cytes inhibition of mitochondrial Ca2+ uptake almost doubled the rate of propagation of the calcium wave across the cell [47] In contrast, in BHK cells ... fusion or ﬁssion (mitofusins, dynaminrelated protein-1) PKC comprises a family of serine-threonine kinases that are involved in the transduction of a wide number of extracellular signals Based on...

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Báo cáo khoa học: " Synergistic effect of ERK inhibition on tetrandrine-induced apoptosis in A549 human lung carcinoma cells" docx

Ngày tải lên : 07/08/2014, 23:22

... the ERK inhibitor PD 98059 on the expression patterns of Akt within A549 cells was examined TET treatment induced apoptosis and resulted in a decrease in Akt and ERK expression PI 3K inhibition had ... by FACS Calibur Flowcytometry (Becton Dickinson, USA) Selective inhibitor study The mitogen-activated protein kinase kinase (MEK1/2) inhibitor PD98059 and PI 3K inhibitor LY294002 were purchased ... 10-14% in the USA [11] Therefore, it is clear that Synergistic effect of ERK inhibition on tetrandrine-induced apoptosis in A549 human lung carcinoma cells Fig The effects of a PI 3K/ Akt inhibitor...

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Báo cáo toán học: "A recurrence for bounds on dominating sets in k-majority tournaments" pps

Ngày tải lên : 08/08/2014, 14:23

... that dominates A2 in G, however by the deﬁnition of ∆B , and since ∆B ∩ DB = ∅ that would be a contradiction, since if DB → A2 in G, then DB → A2 in T (Because the domination of vertices in A2 ... [1] N Alon, G Brightwell, H A Kierstead, A V Kostochka, P Winkler, Dominating sets in k- majority tournaments, J Combinatorial Theory, Ser B 96 (2006), 374–387 [2] P Erd˝s and L Moser, On the representation ... representation of directed graphs as unions of orderings, o Magyar Tud Akad Mat Kutat´ Int K zl (1964), 125–132 o o [3] D.C McGarvey, A theorem on the construction of voting paradoxes, Econometrica...

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Báo cáo y học: "Differential direct effects of cyclo-oxygenase-1/2 inhibition on proteoglycan turnover of human osteoarthritic cartilage: an in vitro study" pptx

Ngày tải lên : 09/08/2014, 07:20

... functional inhibitor of PGE2 production, either by acting directly on the production of cytokines that induce COX in the inflamed tissues [35] or by its preferential intracellular conversion to ... concentrations reached in vivo in patients might be slightly different from the in vitro concentrations in this study because of low concentrations of binding proteins such as albumin (the culture ... aspirin [54] At the concentration tested, celecoxib inhibits NF-κB, an effect also observed for other NSAIDs but only at higher concentrations [55] Inhibition of NF-κB is related to inhibition...

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Effect of cyclooxygenase inhibition on cholesterol efflux proteins and atheromatous foam cell transformation in THP-1 human macrophages: a possible mechanism for increased cardiovascular risk pot

Ngày tải lên : 09/08/2014, 10:20

... conditions: (a) RPMI control, (b) RPMI containing NS398 (10 to 100 μM), (c) RPMI containing indomethacin (0.5, 5, and 50 μM), (d) RPMI containing SC560 (0.001 to 0.1 μM), and (e) RPMI containing ... expression is decreased by the non-specific COX inhibitor indomethacin in a dosedependent fashion in THP-1 monocytes Cultured THP-1 monocytic cells were untreated or exposed to increasing doses of indomethacin ... traditional NSAIDs, although in vitro effects of this inhibition remain to be characterized Conclusion To our knowledge, this is the first study that describes the effects of COX inhibition on reverse...

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Báo cáo khoa hoc:"Effect of the slow (K) or rapid (k +) feathering gene on body and feather growth and fatness according to ambient temperature in a Leghorn × brown egg type cross" ppsx

Ngày tải lên : 09/08/2014, 18:21

... of plumage being at K/ k 12 ± 2.4 + w + k /k 17 ± 3.2 + Periods (weeks) and genotypes w w + + + + K/ k k /k K /k k+ /k+ 6 6 31 ± 9.6 23 ± 6.6 33.8 ± 10.2 43.8 ± 9.9 K/ k – – + 10 w k+ /k+ – – % of ... Experiments and conditions After being kept for weeks on the ﬂoor, two groups of 60 male chicks (30 K/ k+ , 30 k+ /k+ per group) were placed in individual cages in two rooms maintained respectively ... 202 ± 53 K/ k+ k+ /k+ – – 549 ± 82 658 ± 101 787 ± 167 1994 ± 303 521 ± 84 629 ± 114 772 ± 162 1921 ± 338 Feed conversion K/ k+ (FI/∆W) k+ /k+ 31 ◦ C Change of body K/ k+ weight ∆W (g) k+ /k+ – –...

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Báo cáo y học: "Modeling antibiotic and cytotoxic effects of the dimeric isoquinoline IQ-143 on metabolism and its regulation in Staphylococcus aureu" pot

Ngày tải lên : 09/08/2014, 22:24

... MsrA (conferring resistance to macrolides, lincosamides, streptogramins), TetK (conferring resistance to tetracycline), NorA (conferring resistance to fluoroquinolones), VgaAB (conferring resistance ... The clinical S epidermidis strain RP62A was grown in the presence of IQ-143 (concentrations of a quarter of the minimum inhibitory concentration and twice the minimum inhibitory concentration) ... Angiuoli SV, Durkin AS, Haft DH, Vamathevan J, Khouri H, Utterback T, Lee C, Dimitrov G, Jiang L, Qin H, Weidman J, Tran K, Kang K, Hance IR, Nelson KE, Fraser CM: Insights on evolution of virulence...

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Báo cáo y học: "The effect of substance P on asthmatic rat airway smooth muscle cell proliferation, migration, and cytoplasmic calcium concentration in vitro" pdf

Ngày tải lên : 11/08/2014, 03:20

... neurokinin (NK)-1 and NK-2 receptors, leading to potent effects on airway smooth muscle tone and secretions, bronchial circulation, and inflammatory and immune cells [10] Tachykinin levels were increased ... are related to calcium levels in ASMC In asthma, inflammatory cells can release cytokines that in turn induce increased calcium concentration in ASMC, airway smooth muscle contraction, and airway ... (Sigma-Aldrich, Beijing, China) in ml PBS and intraperitoneal (ip) injection of ml heat-killed Bordetella pertussis bacteria (6 × 109/ml, Beijing, China) were administered on day and day Rats in the control...

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Báo cáo y học: "The effect of substance P on asthmatic rat airway smooth muscle cell proliferation, migration, and cytoplasmic calcium concentration in vitro" potx

Ngày tải lên : 11/08/2014, 06:23

... neurokinin (NK)-1 and NK-2 receptors, leading to potent effects on airway smooth muscle tone and secretions, bronchial circulation, and inflammatory and immune cells [10] Tachykinin levels were increased ... are related to calcium levels in ASMC In asthma, inflammatory cells can release cytokines that in turn induce increased calcium concentration in ASMC, airway smooth muscle contraction, and airway ... (Sigma-Aldrich, Beijing, China) in ml PBS and intraperitoneal (ip) injection of ml heat-killed Bordetella pertussis bacteria (6 × 109/ml, Beijing, China) were administered on day and day Rats in the control...

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Báo cáo y học: "Early Characterization of Toll-like receptors in primary lung epithelial cells: strong impact of the TLR3 ligand poly(I:C) on the regulation of Toll-like receptors, adaptor proteins and inflammatory response" ppt

Ngày tải lên : 11/08/2014, 08:21

... chemokines or cytokines found to be elevated by poly(I:C) stimulation including IL-6, IL-8, MIP-3α and GRO-α Interestingly, flagellin also induced IP-10 secretion in SAEC pointing to the induction ... triggered secretion of cytokines and chemokines by SAEC Since the lung epithelium is a major source of chemokine and cytokine secretion in various viral and bacterial pulmonary infections [1,17], ... shown in Fig 1, stimulation of SAEC with the TLR3 ligand poly(I:C) resulted in the most pronounced inflammatory response regarding the secretion of cytokines and chemokines including an increased...

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báo cáo khoa học: " Transient effect of weak electromagnetic fields on calcium ion concentration in Arabidopsis thaliana" potx

Ngày tải lên : 12/08/2014, 03:20

... resonance is affected Conclusion In summary the work presented here shows in Arabidopsis thaliana seedlings transient Ca2+-responses to MF/EMF combinations matching ICR conditions for this ion ... switching the Ca2+-ICR condition on and off Plants usually maintain a cytoplasmatic free Ca2+-ion concentration of 100–200 nM by ion specific membrane channels and storage proteins or organells like ... the standard deviation at the indicated positions following experiments by changing the MF, and the ensuing Ca++ concentrations were again monitored by the aequorin bioluminescence First, the...

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Báo cáo y học: " Effects of body position on autonomic regulation of cardiovascular function in young, healthy adults" pot

Ngày tải lên : 13/08/2014, 14:20

... acquisition system (Chart for Windows) and displayed on a computer monitor, in real time Posture definition Autonomic and cardiovascular functions were measured during prone, supine, and sitting postures ... was little change in total peripheral resistance secondary to vascular compression induced by skeletal muscle contraction, resulting in a minimal change in BP during the sitting posture http://www.chiroandosteo.com/content/15/1/19 ... (following the rest period) to synchronise their breathing to a metronome set at 0.25 Hz (15 times a minute) for minutes Day 1: prone-supine In the prone posture, HR and BP were measured continuously...

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