... S (2004) AAA ATPase p97 ⁄ valosin-containing protein interacts with gp78, a ubiquitin ligase for endoplasmic reticulum- associateddegradation J Biol Chem 279, 45676–45684 18 Latterich M, Frohlich ... & Seeger M (2005) The ubiquitin-domain protein HERP forms a complex with components of the endoplasmicreticulumassociateddegradation pathway J Mol Biol 354, 1021– 1027 25 Ye Y, Shibata Y, Kikkert ... 1218 12 13 containing protein (p97) is a regulator of endoplasmicreticulum stress and of the degradation of N-end rule and ubiquitin-fusion degradation pathway substrates in mammalian cells Mol...
... Ó FEBS 2003 Endoplasmic reticulum- associateddegradation (Eur J Biochem 271) 399 [2-3H]Man (429 GBqÆmmol)1) was from Amersham ... 0.175 mM Glc, labeled for h, and chased in the culture medium (5 mM Ó FEBS 2003 Endoplasmic reticulum- associateddegradation (Eur J Biochem 271) 401 Fig Reglucosylation of Man5GlcNAc2 and Man9GlcNAc2 ... demonstrated for ribophorin I [26] and unstable glycoproteins [6] Ó FEBS 2003 Endoplasmic reticulum- associateddegradation (Eur J Biochem 271) 403 Fig Pattern of oligomannosides released by...
... colocalization with protein disulfide isomerase hints at a localization in the endoplasmicreticulum (Fig 6) although an obvious retrieval signal is missing The human phospholipases D1 and D2 are mainly associated ... cells were transiently transfected with the Hu-K4 cDNA and stained with DAPI (4¢,6-diamidino-2-phenylindole; A,D), with anti-Hu-K4 and anti-rabbit Cy2 (C,D), or with anti-(protein disulfide isomerase) ... luminal or extracellular [17] This domain inherits seven putative glycosylation sites which could only be glycosylated if it enters the endoplasmicreticulum The N-terminal 38 amino acid residues...
... procaspase-8 -associated protein in the endoplasmicreticulum J Cell Biol 139, 327–338 Annaert, W.G., Becker, B., Kistner, U., Reth, M & Jahn, R (1997) Export of cellubrevin from the endoplasmicreticulum ... important for regulating cytoplasmic apoptotic events associatedwith membrane fragmentation To examine proteins that might be potentially associatedwith BAP31, BAP31–Flag was inserted and stably expressed ... position was completely N-terminal acetylated while its counterpart with a Val at position always contained a free N terminus In contrast with many other acetylated proteins, removal of the initiator...
... Arylsulfatase A degradation Fagioli C & Sitia R (2001) Glycoprotein quality control in the endoplasmicreticulum Mannose trimming by endoplasmicreticulum mannosidase I times the proteasomal degradation ... covalent complexes during protein degradation from the ER J Cell Biol 158, 247–257 22 Tokunaga F, Brostrom C, Koide T & Arvan P (2000) Endoplasmicreticulum (ER) -associated degradation of misfolded N-linked ... side chains in ER associateddegradation of mutant ASAs, stably transfected Ltk– cells were incubated with deoxynojirimycin (DNM), an inhibitor of ER glucosidases I and II and with kifunensine...
... defective GABAA receptor subunits also form oligomers with wild-type subunits, leading to the degradation of the latter by ERAD MLD is an autosomal recessive disease because degradation of wtASA induced ... react with moab A2, nor with any other structure-sensitive ASA moab, indicating that it is severely misfolded This allowed an investigation of the folding status of the wtASA within a heteromer with ... deficiency is frequently caused by missense mutations, which cause misfolding and endoplasmic reticulumassociated degradation (ERAD) of the respective ASAs [7] Because the pd allele is so frequent,...
... transfected with CRE firefly luciferase construct were incubated with RIAA (1, and 10 μg/ml) for h followed by LPS (1 μg/ml) stimulation for h The luciferase activities were determined and corrected with ... RAW 264.7 cells (Fig 1B) Next, RAW cells were transfected with either NF-κB or CRE firefly luciferase construct, treated without or with RIAA (1, and 10 μg/ml), and followed by LPS (1 μg/ml) ... calprotectin and PGI-M/TXB2 ratio) commonly associatedwith NSAIDs [6] To gain further understanding of RIAA's anti-inflammatory mechanism (s), we evaluated its effects on cell signaling pathways...
... incubated with [14C]18:1-CoA on its or in the presence of 70 µM lysoPC (acylated with 18:1), without or with 80 µM Ag NO3 Mean values are presented for 2–5 independent experiments, with standard ... diacylglycerol; ER, endoplasmic reticulum; FFA, free fatty acid; GFP, green fluorescent protein; MAM, mitochondria -associated membrane; PA, phosphatidic acid; PAM, plasma membrane associated membrane; ... smaller and occurred only with [1-14C]18:1-CoA Labelling of PE remained unaffected by inclusion of 70 µM of its lyso derivative When plasma membranes were incubated with radiola- Figure [14C]acyl-CoA...
... the EndoplasmicReticulum to the Cytosol Cell 1996, 84:769-779 Yang M, Omura S, Bonifacino JS, Weissman AM: Novel aspects of degradation of T cell receptor subunits from the endoplasmicreticulum ... Vpu-mediated CD4 degradation [24]; 3) Vpu-mediated CD4 degradation is inhibited by specific proteasome inhibitors [24] Vpu-induced CD4 degradation is reminiscent of ER -associated protein degradation ... CD4 in the endoplasmicreticulum (ER) in the form of Env-CD4 complexes and prevents its transport and maturation to the cell surface [7] The accessory protein Vpu induces a rapid degradation...
... screening with kinectin baits Fig 10 Co-immunoprecipitation of kinectin bait D with EF-1δ Fig 11 Specificity of anti-EF-1δ polyclonal antibody Fig 12 Interaction of endogenous kinectin with EF-1δ ... Kinectin anchors EF-1 complex to the ER 5.1 Isolation of EF-1 subunits 147 vi 5.2 Interaction analysis of kinectin with EF-1 subunits using yeast 148 two-hybrid method 5.3 Verification the interactions ... Association of kinectin with EF-1 subunits in intact cells 161 5.6 Characterization of kinectin and EF-1γ binding domain on EF-1δ 161 5.7 Kinectin anchors the EF-1 subunits to the ER 166 5.8 Aberrant...
... situated before any rivers merged with it and Shinsaibashi monitoring station was located after Kuzuha, Mizunashi and Muro rivers along withits other tributaries merged with it Water Sampling and Chemical ... animal husbandry within the study area, its pollution loads were not taken into account River Flow Survey Water of the Hadano basin, on its way from top of the alluvial fan to its center, infiltrates ... of river water quality With this in mind, this study was undertaken to spatially investigate the present status of the river water quality in Hadano basin and its relation with nonpoint sources...
... agreement with the findings of our previous study with synthetic tau peptides [21] Fig Pro216 in tau is important for its interaction with Fyn-SH3 CHO cells were transiently cotransfected with Fyn ... whether the tyrosine phosphorylation status of tau itself is important for its interaction with Fyn-SH2, CHO cells were transiently cotransfected with wild-type tau or a mutant construct in which ... phosphorylation at individual tyrosines in tau is important for its interaction with Fyn-SH2, CHO cells were transiently cotransfected with Fyn together with one of five mutant tau constructs in which single...
... devices The discussion on how ESP_NULL might be used with non-IP network devices is outside the scope of this document In this memo, NULL is used within the context of ESP For further information ... be zero (0) bits, to facilitate interoperability and to avoid any potential export control problems To facilitate interoperability, the IV size for this algorithm MUST be zero (0) bits Padding ... convenient way to represent the optional use of applying encryption within ESP ESP can then be used to provide authentication and integrity without confidentiality Unlike AH these services are not applied...
... Briefly, cells were fixed with 3.7% paraformaldehyde, quenched with 50 mm ammonium chloride for 15 min, and permeabilized with 0.1% Triton X-100 for min, before being treated with 1.5 m hydrochloric ... Disruption of endoplasmicreticulum structure and integrity in lipotoxic cell death J Lipid Res 47, 2726–2737 10 Wang D, Wei Y & Pagliassotti MJ (2006) Saturated fatty acids promote endoplasmicreticulum ... (2007) Signal integration in the endoplasmicreticulum unfolded protein response Nat Rev Mol Cell Biol 8, 519–529 530 37 Zhang K & Kaufman RJ (2008) From endoplasmicreticulum stress to the inflammatory...
... MDL1(60-695), with and without the corresponding Histags, were fixed with 4% paraformaldehyde in 0.1 m sodium cacodylate buffer (pH 7.2) supplemented with 0.8 m sorbitol, mm MgCl2 and mm CaCl2 with or without ... plates without uracil and tryptophan and used for replica plating Selection plates containing SCD without tryptophan were supplemented with gÆL)1 of 5-FOA Resistant colonies appeared within 5–7 ... adsorption method in combination with [c-32P]ATP [52] 0.5 lm of purified MDL1 was incubated with increasing concentrations of ortho-vanadate By addition of buffer supplemented with ATP, the reaction was...
... provides blood with energy to circulate through its respective vascular network While these pumps are pulsatile (i.e blood is delivered into the circulatory system intermittently with each heart ... relationship Because EDFLR of its sensitivity to inotropic agents, the ESFLR 1.50 ESFLR is typically used to index contractile ESFLR with positive Inotropic Agent ESFLR with negative Inotropic Agent ... ventricle with weak, relaxed muscles, as being similar to those of a floppy balloon What would happen to pressure inside a floppy balloon if we were to vary its volume Let's start with no volume...
... A resolution Crystals of rhombohedral shape with dimensions of 0.1 · 0.1 · 0.15 mm were used for data collection of the apo enzyme and its complex with fructose, the product of hydrolysis These ... potential surface with fructose in the active site ‘funnel’, (B) electron density map for fructose and surrounding side-chains, (C) hydrogen bonds with numbering of residues interacting with fructose ... fructosyl units in short-chain inulin-type fructans than 1736 between glucosyl and fructosyl units This enzyme was also able to hydrolyse inulin, but substrate specificity decreased with the increasing...
... cooperation with US and Canada on traumatic brain injury Those priorities are in line with the effort on paediatric brain diseases started in 2011, with the European Pact for Mental Health and with ... legally binding with human hES or iPS cells developed from patients where applicable It should envisage generating models with the intention to investigate diseases variations in relation with different ... research following the Europe 2020 strategy with more industry-driven applied research to boost innovation in the health sector including social innovation Withits many broad, bottom-up topics suited...
... precursor [prosurfactant protein C (proSP-C)] are associatedwith interstitial lung disease (ILD), misfolding of proSP-C in the endoplasmicreticulum (ER), cellular toxicity, and reduced levels ... initially found in proteins belonging to the Bri family, associatedwith familial British and Danish dementia, in chondromodulin, associatedwith chondrosarcoma, and in proSP-C [12] These proteins ... infection associatedwith constitutive expression of misfolded SP-C J Cell Biol 172, 395–407 12 Sanchez-Pulido L, Devos D & Valencia A (2002) BRICHOS: a conserved domain in proteins associated with...
... one copy of SecY associatedwith one SecA and one translocation substrate molecule [21] Two SecY molecules in a nearly front-to-front orientation were proposed to be associatedwith a translating ... reconstituted from purified components of the endoplasmicreticulum membrane Cell 75, 615–630 Simon SM & Blobel G (1991) A protein-conducting channel in the endoplasmicreticulum Cell 65, 371– 380 Crowley ... progress in the field will hinge on structural data, with the ‘holy grail’ being a picture of an active translocon, where a channel associatedwith both a partner and a translocating polypeptide...