... Structures and molecular weights of soyasapogenol B, C and B1 98 Fig 28 LC-MS Chromatogram of standard and purified sample of soyasapogenol B Panels (A and C) are authentic standard and samples of ... 79 6.2.1.5 HPLC Analysis of Soyasaponins and Soy isoflavones 79 6.2.1.6 LC/MS Analysis of Total soyasaponins and Soy isoflavones 79 6.2.2 Comparison of Soyasaponin Concentration Methods ... recovery of soyasapogenols A and B without producing artifacts (Ireland and Dziedzic 1986; Rupasinghe, Jackson et al 2003) Moreover, Ireland and Dziedzic (Ireland and Dziedzic 1986) showed that...
... ethidium bromide and UV irradiation for detection of DNA in gels, thereby preventing DNA damage of the long molecules and enhancing isolationof full-length clones The range of cloning kits and vectors ... ordering process and the cost of synthesis is relatively cheap An example of appropriate design of primers to add attB1 and B2 sites to the 5′ and 3′ ends respectively of a gene of interest are ... N-glycosidic bonds between uracil and deoxyribose leading to a loss of the base The loss of bases leads to disruption of base pairing and therefore exposure of single strands that can be annealed to...
... thankful to Professor Les Copeland (Faculty of Agriculture, Food and Natural Resources - The University of Sydney, New South Wales, Australia) and Professor Kaeko Kamei (Department of Biomolecular ... proteins Generally, the two-step chromatography of AEX and HIC was sufficient for the purificationof FIII-1, FIII-2, and FII, since their SEC and SDS-PAGE profiles represented pure proteins Fraction ... alignment of fragments obtained from MS/MS analyses of FIII-1 and FIII-2 from P excavatus with lumbrokinase and its precursor from L rubellus (sp:P83298 and U25647) and E fetida (gpu:EU167737 and AY438624),...
... Journal 2010, 7:241 http://www.virologyj.com/content/7/1/241 Page of Figure Expression andpurificationof the tgK protein M represented standard protein molecular weight markers The arrow marked the ... http://www.virologyj.com/content/7/1/241 Page of Figure Purificationof the rabbit anti-tgK polyclonal antibody and Western blot assay M represented standard protein molecular weight markers; M1 ... http://www.virologyj.com/content/7/1/241 Page of Figure Indirect immunofluorescent microscopy was used to monitor the DEV antigen distribution in liver, harder’s glands, cecum, spleen and kidney of the infected ducks...
... diagnostics and epidemiologic surveys Methods Strains, plasmids, and enzymes Expression andpurificationof LSV coat protein Figure Expression andpurificationof LSV coat protein A, Expression andpurification ... Alignment of the amino acid sequence of coat protein of different LSV isolates and other carlavirus Figure Alignment of the amino acid sequence of coat protein of different LSV isolates and other ... Laboratory of Arid and Grassland Agroecology of Ministry of Education, School of Life Sciences, Lanzhou University, Lanzhou 730000, China and 4Department of Bacteriology, Graduate School of Medical Science,...
... hexakisphosphate) is the primary storage form of phosphorus in plants They occupy about 1-3% of the seeds of grains and legumes, and 60-80% of the total amount of plant phosphorus (Nelson, 1967) Phosphorus ... PuriWcation and structural characterization Protein Expression andPurification 46, pp.221–232 Bradford, M.M 1976 A rapid and sensitive method for quantitation of microgram quantities of protein ... determination of inorganic orthophosphate and it’s application to the assay of inorganic pyrophosphate Anal Biochem 113:313–317 Kim, Y O., Kim, H K., Bae, K S., Yu, J H and Oh, T K 1998 Purification and...
... surface of the pituitary gonadotrope This leads to the synthesis and secretion of LH and FSH which stimulate the production of steroid hormones in their gonads Testosterone (T), estrogen (E) and ... stimulatory effects of GnRH and activin during puberty and sexual maturation 1.5 Aim The aim of this project is to initiate research on the mechanism of transcriptional regulation of the FSHβ subunit ... responsiveness of the promoter and the sequence will be analyzed to identify putative regulatory elements that may mediate these effects 23 MATERIALS AND METHODS 2.1 Extraction andisolationof genomic...
... decrease of penetration capacity andpurification efficiency during continuous use of the facility was investigated The facility consisted of two tanks In one of the tanks, continuous use of the ... 45 Upper layer Lower layer of weir (cm) (cm) (cm) Akadama sand (5) Pit sand (40) Pit sand (45) Journal of Water and Environment Technology, Vol.3, No.2, 2005 water and Overflow water were measured ... content in pit sand The initial nitrogen content of Akadama sand in Tank A was higher than that of pit sand of Tank B The nitrogen content in both Tanks increased with time In both Tank A and B, the...
... polysaccharide One milligram of the partially purified polymer contained 309.7 µg of protein, 160.2 µg of neutral sugar, 58.5 µg of hexosamine, 30.8 µg of uronic acid and 3.8 µg of phosphorus Constituent ... concentration of ppm to estimate the effect of pH After min, A660 was measured and the extent of flocculation was calculated Pronase digestion of extracellular polymer Forty milligrams of the extracellular ... this study were of reagent grade Results and Discussion Extraction and partial purificationof extracellular polymer The floc was satisfactorily deflocculated within 10 by sonication of the activated...
... for cDNA single strand synthesis, 600 ng of RNA, 0.5 lg of oligo(dT) primer and 10 mm of dNTPs were incubated for at 65 °C Subsequently, lL of the first strand buffer X and lL of dithiothreitol ... mixture of other isoforms (B) Gel visualization of proteins eluted Lane 1, molecular weight markers Numbers on the left indicate the Mr values of the standards in thousands Lanes 2–9, lg of peak ... P III ⁄ P IV and a mixture of other isoforms (*), respectively, in the presence (lanes 2–5) or absence (lanes 6–9) of 2-mercaptoethanol (C) Elution profile of P III and P IV Chromatofocusing chromatography...
... shown) Cloning of anserinase cDNA PCR was performed using a set of primers (A and B) and the first-strand cDNA for 3¢ rapid amplification of cDNA ends (RACE), prepared from total RNA of 6004 Nile ... including 56 bp of 5¢ UTR, 1482 bp of ORF, and 299 bp of 3¢ UTR The coding region of the sequence was translated into 494 amino acids, which included a typical signal peptide of 18 amino acids and two ... total volume of 50 lL containing 0.75 lL of a template, 150 pmol of a forward primer (A), 150 pmol of a reverse primer (B or C), · G-Taq buffer, 10 nmol each of dATP, dGTP, dCTP and dTTP, and 0.5...
... of 50 nmÆmin)1, band-width of 1.0 nm, and a resolution of 0.1 nm in the temperature range between 10 °C and 70 °C, after preincubation for 10 at each temperature Three runs were accumulated and ... of 0.6 lM The data were fitted to Michaelis–Menten curves Squares and dotted line: data and fitting with PHI/PHO ratio of 0.5 Triangles and continuous line: data and fitting with PHI/PHO ratio of ... Asterisks and broken line: data and fitting with PHI/PHO ratio of hypothesis and therefore a direct involvement of PHI in electron transfer This conclusion is consistent with the very low degree of identity...
... intense band of 61 kDa (NrfA) and a band of weak intensity of 19 kDa (NrfH), confirming its hetero-oligomeric nature (Fig 1, lane 1) However, in the absence of boiling (Fig 1A, lanes and 4) high ... and 4) high molecular mass bands of approximately 110 kDa and > 200 kDa were visible, as well as a faint band at 37 kDa, suggesting the presence of dimers All of the bands stained positively for ... between NrfH and NrfA is discussed The reassessment of previous spectroscopic studies was undertaken, particularly regarding the assignment of spectroscopic and redox potentials of the NrfA hemes...
... BLAST of peptide sequences of 21 kDa proteins in databases Tryptic peptides were puri®ed by RP-HPLC and some of them were totally or partially analyzed by Edman sequencing (ááá) and/ or by MALDI-TOF ... BAS1 protein of Brassica, spinach, barley and A thaliana, PR1 of Phaseolus and MHF of A thaliana These proteins belong to the 2Cys-Prx subfamily All plant 2Cys-Prx proteins, except BAS1 of barley, ... previously [21] Chloroplastic thioredoxins m and f from spinach were a kind gift of P Schurmann (University È of Neuchatel, Switzerland) Thioredoxins m and h from à Chlamydomonas were puri®ed as...
... presequence of 19 amino acids The mature protein would have a molecular mass of 41.7 kDa and a pI of 6.34 The YedO protein from E coli and the d-CDes from Arabidopsis showed an overall identity of 36% and ... d-CDes mRNA and protein levels in older plants, during the light phase in a diurnal light ⁄ dark rhythm and under sulfate limitation Results In silico characterization andisolationof the Arabidopsis ... protein has a predicted molecular mass of 43.9 kDa and a pI of 7.2 It contains relatively high amounts of the sulfur amino acids cysteine (four residues) and methionine (10 residues) According...
... which contained 20 ng of first strand cDNA, · PCR buffer (20 mM Tris/HCl pH 8.4, 50 mM KCl), 0.2 lM of each primer, 1.5 mM MgCl2 and 0.2 mM of dNTPs PCR Construction and screening of a grass carp genomic ... KCl), lM of each primer, 0.2 mM of dNTPs, 1.5 mM MgCl2 and U of Taq DNA polymerase (Invitrogen) The PCR program consisted of predenaturation at 94 °C for min, followed by 35 cycles of amplification ... performed on first-strand cDNAs that were reverse transcribed from total RNA by use of Superscript II reverse transcriptase (Invitrogen) and consisted of 20 ng of first strand cDNA, · PCR buffer...
... respectively, and the resultant VNARs evaluated for retention of binding affinity Material and methods Equipment and reagents Restriction enzymes and Vent DNA polymerase were purchased from New England ... possibility of inter- and intra CDR disulphide cross-links These strategies are summarized in Fig 1B and Table 1, and details of the library construction are given in greater detail in the Materials and ... possibility of disulphide bond formation genetically by replacement of residues Cys22 and Cys82 with alanine and valine, respectively, to give a cysteine minus mutant (12F-11DCys) Use of alanine and...