isolation purification and analysis of chondroitin sulfate proteoglycans
Báo cáo khoa học: Calcium-induced activation and truncation of promatrix metalloproteinase-9 linked to the core protein of chondroitin sulfate proteoglycans pot
... side is shown the position of the 225 and 92 kDa forms of proMMP-9 in serum-free culture medium of THP-1 cells and the 72 kDa form of proMMP-2 in serum-free culture medium of human skin fibroblasts ... addition of 10 mM EDTA to the reaction mixture At the right is shown the position of the 225 and 92 kDa forms of proMMP-9 in serum-free culture medium of THP-1 cells and the 72 kDa form of proMMP-2 ... the stacking and the separating gel At the left side is shown the position of the 225 and 92 kDa forms of proMMP-9 in serum-free culture medium of THP-1 cells and the 72 kDa form of proMMP-2 in...
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Isolation, purification and detection of soyasaponins and their associated bioactivities in cultured hepatocarcinoma cells
... Structures and molecular weights of soyasapogenol B, C and B1 98 Fig 28 LC-MS Chromatogram of standard and purified sample of soyasapogenol B Panels (A and C) are authentic standard and samples of ... Temperature 79 6.2.1.5 HPLC Analysis of Soyasaponins and Soy isoflavones 79 6.2.1.6 LC/MS Analysis of Total soyasaponins and Soy isoflavones 79 6.2.2 Comparison of Soyasaponin Concentration ... the complex structure, overlap polarities, artifacts (Zhang and Popovich 2009) and labor intensive purification, isolation and analysis of these compounds (Kitagawa, Yoshikawa et al 1982; Shiraiwa,...
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Expression analysis and functional significance of chondroitin sulphate proteoglycans and heparan sulphate proteoglycans in prostrate cancer
... remodelling of chondroitin/ dermatan sulphate 25 and heparan sulphate chains Page | iv Table of contents 1.2.4 Functions of chondroitin/ dermatan sulphate and heparan 26 sulphate and/ or proteoglycans ... addition of GlcA and GlcNAc N-deacetylation and N-sulphation of GlcNAc units N-deacetylation and N-sulphation of GlcNAc units N-deacetylation and N-sulphation of GlcNAc units N-deacetylation and N-sulphation ... Functions of chondroitin/ dermatan sulphate and heparan sulphate and/ or proteoglycans in cancer The huge repertoire of structurally diverse chondroitin/ dermatan sulphate proteoglycans and heparan...
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studies of pathogenesis-related proteins in the strawberry plant partial purification of a chitinase-containing protein complex and analysis of an osmotin-like protein gene
... PARTIAL PURIFICATION OF A CHITINASE-CONTAINING PROTEIN COMPLEX IN THE STRAWBERRY PLANT……………………………32 ISOLATION OF AN OSMOTIN-LIKE PROTEIN GENE FROM STRAWBERRY AND ANALYSIS OF THE RESPONSE OF THIS ... chitinase caused a great loss of activity (Andersen et al., 1997; Iseli-Gamboni et al., 1998) In addition, mutation of Tyr 123 of a Zea mays chitinase and a similar tyrosine of an Arabidopsis chitinase ... rearranged after freezing and thawing, which led to the exposure of new Ca++-binding sites Binding of Ca++ to these sites caused inhibition of the antifreeze and chitinase activities of these complexes...
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Review and analysis of renewable energy perspectives in Serbia
... [14] Statistical Office of the Republic of Serbia National Accounts of Serbia, Gross Domestic Product of the Republic of Serbia (1997-2007) Statistical Office of the Republik of Serbia, 2009 [15] ... the fields of energy policy and modelling, development & administration of DSS and in lectures and seminars of the NTUA and the Technical Chamber of Greece regarding energy policy and DSS He ... for RES, Ministry of Mining and Energy of the Republic of Serbia, whose helpful remarks and fruitful observations were invaluable for the development of this work The content of the paper is the...
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E-Recruiting Categories and Analysis of Fortune 100 Career Web Sites
... identification of hiring needs; submission of job requisition and approval; job posting, submission of job applications; screening of résumé/application; interviewing; pre-employment screening; and job offer ... career needs of executives, managers, and professionals, leveraging the Wall Street Journal brand CareerJournal.com provides recruiters and job seekers with a database of job openings and résumés, ... recruiters and job seekers a better understanding of the erecruiting methods, we surveyed a wide range of recruiting sources Based on this analysis, we identify six basic categories of the e-recruiting...
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Purification and cloning of PCR products
... ethidium bromide and UV irradiation for detection of DNA in gels, thereby preventing DNA damage of the long molecules and enhancing isolation of full-length clones The range of cloning kits and vectors ... ordering process and the cost of synthesis is relatively cheap An example of appropriate design of primers to add attB1 and B2 sites to the 5′ and 3′ ends respectively of a gene of interest are ... N-glycosidic bonds between uracil and deoxyribose leading to a loss of the base The loss of bases leads to disruption of base pairing and therefore exposure of single strands that can be annealed to...
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Đo kiểm đánh giá can nhiễu mạng truyền hình cáp The Measurement and Analysis of the effect of interference in Television Cable network
... SCHWARZ - SOUND and TV BROADCASTING – CCIR and FFC tv standards – Printed in the federal republic of Germany 18 3GPP2 C.S0011-C, version 2.0 – Recommended Minimum Performance Standards for cdma2000 ... coding and modulation for cable systems – European Broadcasting Union 13 ETSI EN 302 307 V1.2.1 (2009-08) – Digital Video Broadcasting (DVB); Second generation framing structure, channel coding and ... channel coding and modulation systems for Broadcasting, Interactive Services, News Gathering and other broadband satellite applications (DVB – S2) 14 Tim Williams – EMC for Product Designers – Newnes...
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Báo cáo khoa học: Functional dissection of Escherichia coli phosphotransacetylase structural domains and analysis of key compounds involved in activity regulation potx
... from CONICET and ´ ´ Agencia Nacional de Promocion Cientı´ fica y Tecnologica M F Drincovich and C S Andreo are members of the Researcher Career of CONICET, and V A Campos-Bermudez and F P Bologna ... phosphotransacetylase Results Expression and purification of E coli Pta and truncated Ptas containing the C-terminal end By analysis of the protein domain architecture of E coli Pta, three conserved domains ... obtained for Pta-F2 and Pta-F1 (B) Activation of the acetyl phosphate synthesis activity of E coli Pta by different concentrations of pyruvate and PEP Results are presented as percentage of activity...
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Báo cáo khoa học: Staphylococcus aureus elongation factor G – structure and analysis of a target for fusidic acid pdf
... helices AV and BV at the surface of domain V Gly621 and Gly617 are in the area of contact with the 1095 and 2473 regions of 23S RNA The two helices are facing the ribosome, and the four-stranded b-sheet ... type and various mutants, domains III, IV and V display a movement relative to domains I and II, resulting in a shift of the ˚ tip of domain IV of up to A [14,16] The ribosomebound structure of ... 666 and 670, within one b-strand of domain V, are also important for interdomain interactions The N-terminal part of the strand interacts with the N-terminal part of helix CG in domain G, and...
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Báo cáo khoa học: Functional expression of the quinoline 2-oxidoreductase genes (qorMSL) in Pseudomonas putida KT2440 pUF1 and in P. putida 86-1 Dqor pUF1 and analysis of the Qor proteins doc
... from Hanes plots Determination of the metal contents of the Qor proteins, and detection of the nucleotide moiety of the molybdenum cofactor The contents of molybdenum and iron were determined by ... copy of the qor genes and their flanking regions The plasmid pBG3a was digested with XhoI and DraIII to remove nptII After the removal of the 3¢ overhang and the filling of the 5¢ overhang of the ... cleaves 364-bp upstream of the start codon of qorM, and DraIII, that cleaves 8-bp downstream of the stop codon of qorL After removing the 3¢ overhang and filling the 5¢ overhang of the plasmid with...
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Mechanics and Analysis of Composite Materials ppt
... MECHANICS AND ANALYSIS OF COMPOSITE MATERIALS MECHANICS AND ANALYSIS OF COMPOSITE MATERIALS Valery V Vasiliev Professor of Aerospace Composite Structures Director of School of Mechanics and Design ... ones which usually cover not only mechanics of materials but also include analysis of composite beams, plates and shells, joints, and elements of design of composite structures that, being also ... nonlinear elastic and elastic-plastic analysis demonstrating specific types of behavior of composites with metal and thermoplastic matrices Chapter is concerned with mechanics of laminates and includes...
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16 - visualization and analysis of email networks
... v}, and let du and dv be the degree centrality values of nodes u and v respectively We can appoint u as a source of the edge if du > dv , and v as a target If du > dv , then v is the source and ... network analysis is a measure of the importance of a node embedded in the network Hierarchical layout is popularly used to visualize centrality analysis of a network This involves higher placement of ... hierarchical layout makes the graph easier to navigate and facilitates the understanding of the structure of the network from the perspective of the centrality measure mapped to the edge directions...
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The State of Microfinance Investment 2012 MicroRate’s 7th Annual Survey and Analysis of MIVs potx
... 2021 2050 -8 THE STATE OF MICROFINANCE INVESTMENT 2012 th MicroRate’s Annual Survey and Analysis of MIVs Concentration and diversification The 2009-10 microfinance crisis and the investment turnover ... major source of funding for millions of microentrepreneurs in some of the world’s poorest regions 12 THE STATE OF MICROFINANCE INVESTMENT 2012 th MicroRate’s Annual Survey and Analysis of MIVs 13 ... www.eib.org/products/microfinance/index.htm About the Grand-Duchy of Luxembourg The Grand-Duchy of Luxembourg was among the first countries in the EU to recognize the importance and the value of microfinance...
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detection and analysis of genetic alterations
... follicle Sebaceous gland Sweat gland Fat Figure Histological section of normal epidermis (A) and a schematic picture of the different layers of the skin (B) 25 Detection and analysis of genetic alterations ... more intricate, and thus more easily influenced folding of the strands (Highsmith, 1999) Detection and analysis of genetic alterations in normal skin and skin tumours The detection of fragments ... advantages of lower template requirement, generation of single stranded DNA without time-consuming denaturation of double-strands and ease of automation (Innis, 1988; Murray, 1989) Manipulations of the...
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high resolution separation and analysis of biological macromolecules, part a
... to the analysis of enzymatically derived mixtures of peptides and also for the analysis of synthetically derived peptides An example of the high-resolution analysis of a tryptic digest of bovine ... focus of current studies on the conformational analysis of peptides and proteins, the development of new optimization procedures, and the design of new ligands There is no doubt that the demands of ... tool in the analysis and isolation of peptides and proteins, particularly in the field of analytical biotechnology The widespread opportunities offered by RP-HPLC have been greatly expanded through...
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high resolution separation and analysis of biological macromolecules, part b
... to choice of ligand, ligand attachment, buffers, and sample recovery techniques were reviewed A specific example of AFC of peptides offered was that of examining the binding affinities of multiple ... S Hodges and C T Mant, in "HPLC of Peptidcs and Proteins: Separation, Analysis and Conformation" (C T Mant and R S Hodges, eds.), p CRC Press, Boca Raton, FL 1991 [l] OF PEP'IIDES ANALYSIS BY ... T Mant, and R S Hodges, in "HPLC of Peptides and Proteins: Separation, Analysis and Conformation" (C T Mant and R S Hodges, eds.), p 307 CRC Press, Boca Raton, FL, 1991 Ill ANALYSIS OF PEPTIDES...
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stochastic modeling and analysis of telecoms networks
... noted that the decryption of standards of thousands of pages and their translation into human language require much work and fine knowledge in a wide variety of disciplines, and as well as infinite ... Modeling and Analysis A model is also often a support for simulation, in this way it creates an artificial simplification of reality If this method gives very often only approximate results and is ... should the model be fractal or multi-fractal and most of all what is the impact of this form of traffic on the size of the queues? After 10 years of frantic research, we know how to explain the...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose