isolation pcr and nucleotide sequencing
Báo cáo y học: " Functions, structure, and read-through alternative splicing of feline APOBEC3 genes" ppt
... DNA isolation, PCR, and nucleotide sequencing The appropriate well of a 384-well plate was picked using a culture loop and streaked across LB agar plates containing 12.5 μg/ml chloramphenicol and ... sequence relative to primates (human and chimpanzee), rodents (mouse and rat), and carnivores (cat and dog) and will likely provide new insights into disease inheritance and the relationship between ... to itself Gene modeling studies using the predicted nucleotide and amino acid sequences of cat A3 and A3H cDNAs and the programs Spidey [25] and Genewise [26] demonstrated the presence of three...
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Báo cáo khoa học: Purification, characterization, cDNA cloning and nucleotide sequencing of a cellulase from the yellow-spotted longicorn beetle, Psacothea hilaris ppt
... and subjected to gas phase protein sequencing (model LF-3400 DT; Beckman) cDNA cloning, genomic PCR and sequencing A QuickPrepÒ Micro mRNA Purification Kit (Amersham Bioscience) was used for isolation ... without reducing agent and incubated at 33 °C for 30 to load an SDS/polyacrylamide gel The SDS/ PAGE analysis detected three proteins bands by CBB staining and one activity band by activity staining ... 1, standard sugars: G1, G2, G3, G4, cellopentaose (G5) and cellohexaose (G6) Lanes 2–5, G2–G5 treated with P hilaris cellulase at 37 °C for h and cellopentaose were degraded to cellobiose and...
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Báo cáo khoa học: "Isolation and identification of Escherichia coli O157:H7 using different detection methods and molecular determination by multiplex PCR and RAPD" docx
... applied for further determination by multiplex PCR and PCR for flagellar H7 antigen detection DNA preparation for Multiplex PCR, flagellar H7 PCR and RAPD analysis E coli O157:H7 strains which ... was collected and used as a template Multiplex PCR for stx1, stx2, eaeA, and hlyA, and the flagellar H7 gene amplification Multiplex PCR for the detection of stx1, stx2, eaeA, and EHEC hlyA gene ... GeneAmp PCR thermocycler (Model 2400, Perkin-Elmer, USA) Oligonucleotide primers for Stx1, Stx2, eaeA, and hlyA were synthesized as previously described [14] Oligonucleotide sequence of primers and...
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báo cáo khoa học: " Isolation, identification and expression analysis of salt-induced genes in Suaeda maritima, a natural halophyte, using PCR-based suppression subtractive hybridization" doc
... [33,34], and identification of the convergent and divergent pathways between salinity and other abiotic stress responses and the nodes of signalling convergence may greatly enhance the understanding ... set of qRT -PCR reaction for comparison and normalization of the data RNA was isolated as and when required from the leaves of the control and NaCl treated plants as described above and treated ... cDNA libraries, forward and reverse, were prepared using PCRselect-cDNA SSH kit (Clontech, Palo alto, USA) For this, the double stranded cDNAs prepared from the control and NaCl treated samples...
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báo cáo khoa học: " An optimized grapevine RNA isolation procedure and statistical determination of reference genes for real-time RT-PCR during berry development" docx
... increasing our understanding of signalling and metabolic pathways underlying developmental and cellular processes Realtime RT -PCR is currently one of the more powerful and sensitive techniques ... genes were ranked based on their deviation from the mean (2× standard deviation) (Table 3) Highest ranked were SAND and actin in 2003 and 2004 pericarp, respectively, with minimal deviation around ... by actin, EF1-α, and SAND Due to seasonal differences observed using the different approaches (Figure 1; Tables and 3), further validation was initiated to test whether the top candidates continued...
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Báo cáo khoa học: Anti-HIV-1 activity of 3-deaza-adenosine analogs Inhibition of S-adenosylhomocysteine hydrolase and nucleotide congeners pot
... Emin À þ hEINT i ¼ protein 2 Nligand kB T ð2Þ À Eligand À Emin complex and as Ncomplex ¼ Nprotein + Nligand, min hEINT i ¼ DE ¼ Emin complex À Eprotein À Eligand hydrolase, which differed with ... penicillin, and 100 lgÆmL)1 streptomycin, and 5% CO2 at 36 °C AA-2 cells (AK–, dCK–), which lack adenosine kinase and deoxycytidine kinase, were obtained through the AIDS Research and Reference ... 3¢-ketocyclopent-4¢-enyladenine for DZNep and NepA, and adenosine for DZA, DZAri, DZNAri, ddAdo, ddDZA, and ddDZAri using superimposed human (PDB accession no 1A7A) and rat (PDB accession no 1D4F) AdoHcy...
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Báo cáo khoa học: Isolation, characterization and expression analysis of a hypoxia-responsive glucose transporter gene from the grass carp, Ctenopharyngodon idellus potx
... GLUT1 and GLUT3 (exons 3–8), and four in human GLUT2 (exons 7–10) and GLUT4 (exons 6–9) (Table 1) The codons for arginine (96) and valine (231) in gcGLUT (Fig 2) are split between exons and 5, and ... incomplete GLUT ORF and lacked the start codon Using 5¢- and 3¢-RACE PCR, two overlapping full-length cDNA clones of 2.1 kb (GT-cDNA1) and 3.1 kb (GT-cDNA2) that shared 100% nucleotide sequence ... (between exon and exon 5) and valine (between exons and exon 7) are shown Exonic regions are shown in uppercase and intronic regions are in lowercase The split codons are boxed and highlighted...
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Báo cáo " Isomeranzin against Herpes simplex virus in vitro from Clausena heptaphylla (Roxb.) W. & ARN.: Isolation, structure and biological assay " potx
... Dr Tran Ngoc Ninh, Vietnamese Academy of Science and Technology Extraction and isolation Leaves of Clausena heptaphylla were dried at 40 ÷ 500C and powdered The powder (500 g) was extracted with ... ml of saline (for virus control and cell control) and given ml 2x MEM The cells were incubated for 72 hours and observed for cytopathic effects from the virus and cytotoxic effects from the samples ... MeOH 900 at 800C The extract was filtered and concentrated in vacuo to yield 84 g extract The concentrated extract was fractionated into Hexan, EtOAc, BuOH, and H2O-soluble fractions, sequentially...
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fattorusso - modern alkaloids - structure, isolation, synthesis and biology (wiley, 2008)
... Glycosidase-Inhibiting Alkaloids: Isolation, Structure, and Application 111 Naoki Asano Introduction 111 Isolation and Structural Characterization 111 Deoxynojirimycin and Related Compounds 112 Isolation from Morus ... microbes and against desiccation; formation of a thick bark in roots and stems against water loss, microbes, and herbivores; development of spines, thorns, hooks, trichomes, and glandular and ... spp (Moraceae) 112 Isolation from Thai Medicinal Plants ‘‘Thopthaep’’ and ‘‘Cha Em Thai’’ 113 a-Homonojirimycin and Related Compounds 115 Isolation from Garden Plants 115 Isolation from the Thai...
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báo cáo hóa học:" Validation of a HLA-A2 tetramer flow cytometric method, IFNgamma real time RT-PCR, and IFNgamma ELISPOT for detection of immunologic response to gp100 and MelanA/MART-1 in melanoma patients" doc
... study, and analyzed data for ELISPOT and RT -PCR studies VT, reviewed the manuscript and led the ELISPOT study CS, led the RT -PCR study KD, acquired and analyzed tetramer data LA, acquired and analyzed ... Y-intercept (40.155), and Correlation Coefficient (1.000) Standard curve was determined on TaqMan plates and no significant differences were found Spike and recovery TIL 1520 and TIL1235 spiked ... stimulated with irrelevant peptide and HIV had very low background signal and % CV was high, as expected Accuracy, spike and recovery, and LOQ Due to the lack of a reference standard material to establish...
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Báo cáo sinh học: " Development of a real-time RT-PCR and Reverse Line probe Hybridisation assay for the routine detection and genotyping of Noroviruses in Ireland" pdf
... 101 for GI and × 107 to × 101 for GII (Fig 1A and Fig 2A) Melting curve analysis identified positive samples by large peaks at ~90°C and ~88°C respectively for GI and GII NoV (Fig 1C and Fig 2C) ... plasmid DNA for GI NoV and × 107 to × 101 for GII NoV The R2 values for both standard curves were 1.00 with a slope of -3.5 and -3.7 respectively for GI and GII NoV (Fig 1B and Fig 2B) Melting curve ... RNA and µl of 75 pmole random hexamers (Roche) are added to a 0.5 ml PCR reaction tube, mixed and heated to 95°C for A master-mix was prepared according to the manufacturer's instructions and...
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Báo cáo sinh học: " Comparison of real-time PCR and hemagglutination assay for quantitation of human polyomavirus JC" pptx
... employing microtiter and transwell plates Semiquantitative polymerase chain reaction (PCR) and quantitative real-time PCR have been recently developed and employed for the detection and quantitation ... 350714 and Y = × 106X + × 107 and the r2 were 1.0 and 0.95 for JCV(Mad1) virus stocks I and II, respectively Page of (page number not for citation purposes) Virology Journal 2006, 3:3 and inter-run ... Hou J, Fahle G, Fischer S, Major EO: Comparison of PCR- southern hybridization and quantitative real-time PCR for the detection of JC and BK viral nucleotide Page of (page number not for citation...
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Báo cáo hóa học: " Development of a real-time RT-PCR and Reverse Line probe Hybridisation assay for the routine detection and genotyping of Noroviruses in Ireland" ppt
... 101 for GI and × 107 to × 101 for GII (Fig 1A and Fig 2A) Melting curve analysis identified positive samples by large peaks at ~90°C and ~88°C respectively for GI and GII NoV (Fig 1C and Fig 2C) ... plasmid DNA for GI NoV and × 107 to × 101 for GII NoV The R2 values for both standard curves were 1.00 with a slope of -3.5 and -3.7 respectively for GI and GII NoV (Fig 1B and Fig 2B) Melting curve ... RNA and µl of 75 pmole random hexamers (Roche) are added to a 0.5 ml PCR reaction tube, mixed and heated to 95°C for A master-mix was prepared according to the manufacturer's instructions and...
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báo cáo hóa học:"Comparison of real-time PCR and hemagglutination assay for quantitation of human polyomavirus JC" potx
... employing microtiter and transwell plates Semiquantitative polymerase chain reaction (PCR) and quantitative real-time PCR have been recently developed and employed for the detection and quantitation ... 350714 and Y = × 106X + × 107 and the r2 were 1.0 and 0.95 for JCV(Mad1) virus stocks I and II, respectively Page of (page number not for citation purposes) Virology Journal 2006, 3:3 and inter-run ... Hou J, Fahle G, Fischer S, Major EO: Comparison of PCR- southern hybridization and quantitative real-time PCR for the detection of JC and BK viral nucleotide Page of (page number not for citation...
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Báo cáo nghiên cứu nông nghiệp " Isolation, inbreeding and relationships in forest trees " pdf
... Gympie, Queensland outcrossed selfed Forestry and Forest Products Inbreeding problems Petford Vietnam landrace Petford Rwanda landrace E camaldulensis: natural seed source vs local land race Australian ... Isolation, inbreeding and relationships in forest trees Phenotype Selection (seed orchards, seed prod areas) Forestry and Forest Products Why is selection ... source vs local land race Australian seedlot (collected from natural stand, one year younger) Vietnam land race Forestry and Forest Products Open-pollinated eucalypt family insects are the main...
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Báo cáo y học: "Contribution for new genetic markers of rheumatoid arthritis activity and severity: sequencing of the tumor necrosis factor-alpha gene promoter" docx
... of the TNF-α gene, between nucleotides -1,318 and +49, of 554 patients with RA from 11 Portuguese rheumatology centers serving the mainland and Azores and Madeira Islands An association was found ... the 238, -308, -857, and -863 positions, and systemic manifestations, functional status, radiological damage, work disability, and joint surgeries and between haplotypes and radiological damage ... (-857, -308, and -238) and systemic manifestations, radiological progression, work disability, and joint surgeries A weak association was also observed between other SNPs (-863 and -238) and DAS28,...
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Rapid detection of epidermal growth factor receptor mutations with multiplex PCR and primer extension in lung cancer pdf
... instructions Multiplex PCR and primer extension analysis of mutations in EGFR-216 promoter region and exons 18, 19, 20, and 21 Multiplex PCR was used to amplify the promoter region and exons 18-21 of ... primers and conditions used for the multiplex PCR were Page of the same as the PCR described above After multiplex PCR amplification, the PCR products were purified to remove the remaining primers and ... reaction tube Multiplex PCR- SSCP- or PCRARMS-based methods can also simultaneously detect several mutations However, PCR- SSCP require further confirmation by direct sequencing, and PCR- ARMS require...
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báo cáo khoa học: "Construction and EST sequencing of full-length, drought stress cDNA libraries for common beans (Phaseolus vulgaris L.)" doc
... MS and SS and constructed full-length cDNA library and did preliminary sequencing and analysis All authors read and approved the manuscript Acknowledgements: We are grateful to José Polonia and ... resultant double-stranded cDNAs were digested with BamHI and XhoI, and ligated into the BamHI and SalI sites of a Lambda-based pFLCIII-cDNA vector [21] During the first strand cDNA synthesis an ... three different soil treatments and under both drought-stress and irrigated conditions and that whole plants (above and below ground parts) were harvested at seven and three timepoints for the cDNA...
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báo cáo khoa học: " Identification and characterization of wheat long non-protein coding RNAs responsive to powdery mildew infection and heat stress by using microarray analysis and SBS sequencing" ppsx
... YY and DQ carried out the microarray analysis and small RNA sequencing, participated in the long npcRNA identification, and draft the manuscript MX and ZH carried out ChIP analysis, NS, CX and ... 10b), and negative correlation in expression levels was observed between sense strand and antisense strand expression patterns in both JD8 and JD8-Pm30 (Figure 10) In addition, three long npcRNAs, ... long npcRNAs are not conserved among the plant species and responsive to both biotic and abiotic stresses By using combination of microarray and SBS sequencing, a total of 125 putative long npcRNAs...
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Báo cáo khoa học: "Genomic variability in Potato virus M and the development of RT-PCR and RFLP procedures for the detection of this virus in seed potatoes" ppt
... distinct groups - group I and II Group I consisted of PVM isolates detected and characterized in Italy, Germany, China, Poland and Russia and group II consisted of Canadian and US isolates Isolates ... each case RT -PCR amplicons of 520 bp were obtained and digested into 150 and 370 bp fragments upon treatment with MscI (Table 1, Fig 1) Retested samples that gave the expected RT -PCR and RFLP results ... of PCR products by restriction analysis PCR amplicons (520 bp) were produced in RT -PCR using primers PVM3 and PVM4 from RNA extracted from PVM-Ca508 infected tuber (lanes 2), leaf (lane 4) and...
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