isolation pcr amplification and dna sequencing
báo cáo khoa học: " AtMRP6/AtABCC6, an ATP-Binding Cassette transporter gene expressed during early steps of seedling development and up-regulated by cadmium in Arabidopsis thaliana" doc
... lineage of heterozygous T -DNA insertion mutants and were designated as Col-0 in the following The T -DNA insertion site was confirmed by DNA sequencing The presence of only one T -DNA insertion site was ... the dry weight of samples GSH, γ-EC and Phytochelatin levels GSH, γ-EC and PC levels in roots and leaves of Cd-treated and untreated Atmrp6.1 and Atmrp6.2, and corresponding wild-type plants were ... nomenclature proposed by Martinoia and col [32]) was directly cloned by RT -PCR using MR06-NotStart and MR06R-StopNot oligonucleotide primers (table 1) and a full-length cDNA of 4398 bp was obtained...
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Báo cáo y học: "Trace Elements, Heavy Metals and Vitamin Levels in Patients with Coronary Artery Diseas"
... and serum cholesterol levels were not significantly different in patients with and without CAD (Table 1) Comparisons of the levels of the vitamins (retinol, tocopherol and cholecalciferol), and ... levels and mortality Arch Intern Med 2002; 162: 2443-2449 31 Rock E, Winklhofer-Roob BM, Ribalta J, et al Vitamin A, vitamin E and carotenoid status and metabolism during ageing: functional and ... years have led to a good understanding of their mode of action and why they are essential to life (7) Some studies have assessed the association between iron status and CAD risk but the results...
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Báo cáo y học: "Association between regulated upon activation, normal T cells expressed and secreted (RANTES) -28C/G polymorphism and asthma risk – A Meta-Analysis"
... Table and Figure 1) We further performed stratified analysis according to asthma type (atopic asthma and non-atopic asthma), age (children and adult) and ethnicity (Asian and Caucasian and African ... heterogeneity, and it was considered significant for P < 0.01 We used the fixed-effects model and the random-effects model based on the Mantel-Haenszel method and the DerSimonian and Laird method, ... cases and 1766 controls for -28C/G Materials and Methods Identification and Eligibility of Relevant Studies Eligible studies were identified by searching the electronic literature PubMed and EMBASE...
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Báo cáo y học: "Comparative study of serum Na+ and K+ levels in senile cataract patients and normal individuals"
... senile cataract (79 female, 76 male) and the control one consisted of 155 of cases’ close relatives (79 female, 76 male) Mean age and standard deviation of patients and control group were 61.96±14.5, ... range of patients and controls were 44-85 year and 43-86 year respectively Table shows serum Na+, K+ of case and control groups, with a significant difference in serum + Na of case and control ones ... hypertension and any other systemic disease Then positive ones of both groups were excluded from the study Case and control groups were both examined by an expert ophthalmologist and routine ophthalmic...
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Tài liệu Passwords and Privilege Levels pdf
... takes precedence and the enable password command is ignored Many organizations begin using the insecure enable password command, and then migrate to using the enable secret command Often, however, ... the show privilege command, and changing privilege levels can be done using the enable and disable commands Without any arguments, enable will attempt to change to level 15 and disable will change ... privilege—zero, user, and privileged Zero-level access allows only five commands—logout, enable, disable, help, and exit User level (level 1) provides very limited read-only access to the router, and privileged...
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Tài liệu Proposal for a DIRECTIVE OF THE EUROPEAN PARLIAMENT AND OF THE COUNCIL on Alternative Investment Fund Managers and amending Directives 2004/39/EC and 2009/…/EC ppt
... efficiency and investors stem primarily from the conduct and organisation of the AIFM and certain other key actors in the fund governance and value-chain (depositary bank where relevant and valuation ... type of assets and the use of leverage; redemption policy in normal and exceptional circumstances; valuation, custody, administration and risk management procedures; and fees, charges and expenses ... of interest and failures in fund governance, in particular with respect to remuneration, valuation and administration Market efficiency and integrity • Impact of dynamic trading and short selling...
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OUTDOOR AIR POLLUTION: ASSESSING THE ENVIRONMENTAL BURDEN OF DISEASE AT NATIONAL AND LOCAL LEVELS potx
... cardiovascular and respiratory disease, and among infants Morbidity outcomes include hospitalization and emergency room visits, asthma attacks, bronchitis, respiratory symptoms, and lost work and school ... rates has been observed in areas and cities in both cold (Detroit and Montreal) and warm (Mexico City and Bangkok) climates; where PM peaks in the summer (Steubenville and Philadelphia), winter (Utah ... mobile (e.g cars, trucks and buses) and stationary (e.g power plants and boilers) sources, but other sources such as road dust, biomass burning, manufacturing processes and primary pollutants from...
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Educating the Net Generation by Diana G. Oblinger and James L. Oblinger, Editors ppt
... research-extensive land-grant institution with 30,000 students and approximately 2,200 full- and part-time faculty Previously, he served as provost and executive vice chancellor, dean and executive ... music Jake Seaton is a big arts and entertainment fan He lives and breathes for music, movies, and anything Hollywood He can tell you about film and music history and can quote even the most obscure ... favor more graphics, a rapid pace, and immediate responses If faculty and administrators can understand the factors that lead to student success—which persist and which differ from their own college...
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Báo cáo khoa học: Staphylococcus aureus elongation factor G – structure and analysis of a target for fusidic acid pdf
... II and III, the only physical connection between domains G and II and domains III, IV and V In the S aureus EF-G structure, this loop has a bent conformation, and packs against domain III and ... helices AV and BV at the surface of domain V Gly621 and Gly617 are in the area of contact with the 1095 and 2473 regions of 23S RNA The two helices are facing the ribosome, and the four-stranded b-sheet ... of molecules A and B form an extended b-sheet, and helix AV packs in an antiparallel fashion to the equivalent helix in molecule B Residues 2–38, 64–441 and 445–692 in molecule A and residues 2–41,...
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Báo cáo Y học: A novel DNA repair enzyme containing RNA recognition, G-patch and specific splicing factor 45-like motifs in the protozoan parasite Toxoplasma gondii potx
... introns and three exons, which was confirmed by the isolation and sequencing of the fulllength TgDRE cDNA The comparison of cDNA and genomic DNA nucleotide sequences allowed the determination ... pBluescript-SK+ and sequenced using an ALFexpress automated sequencer (Amersham Pharmacia Biotech) Rapid amplification of cDNA ends (RACE) and sequencing To obtain full-length cDNAs, a RACE -PCR technique ... control (Fig 2A,B) The RT -PCR revealed a band of 1.4 kb corresponding to TgDRE in both tachyzoite and bradyzoite stages of T gondii The cloning and sequencing of this RT -PCR product confirmed its...
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LEAVING CERTIFICATE A CCOUNTING SYLLABUS: Ordinary and Higher Levels potx
... they arise and the need to make provision for them o understand depreciation and the basic principles of the appropriate standard o understand wear and tear and obsolescence o understand t w o ... of profit and consequently the balance sheet o understand VAT and the basic principles of the appropriate standard o understand what accruals, prepayments and provisions are o understand what ... practical application and which develops in each student numeracy and a knowledge and understanding of business and enterprise through the use of figures, computations and statistics 5.5 To contribute...
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Malnutrition - Quantifying the health impact at national and local levels doc
... Sudan, Yemen EUR A Andorra, Austria, Belgium, Croatia, Czech Republic, Denmark, Finland, France, Germany, Greece, Iceland, Ireland, Israel, Italy, Luxembourg, Malta, Monaco, Netherlands, Norway, Portugal, ... diet and disease, in turn, are closely linked to the general standard of living, the environmental conditions, and whether a population is able to meet its basic needs such as food, housing and ... inadequate food intake and/ or the consumption of non-nutritious food, and thus to malnutrition On the other hand, malnutrition itself can have far-reaching impacts on the environment, and can induce...
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Searchlights on Health by B. G. Jefferis and J. L. Nichols pptx
... extent startling, is chaste, practical and to the point, and will be a boon and a blessing to thousands who consult its pages The world is full of ignorance, and the ignorant will always criticise, ... Williams, and the Online Distributed Proofreading Team SEARCHLIGHTS ON HEALTH THE SCIENCE OF EUGENICS A Guide to Purity and Physical Manhood Advice to Maiden, Wife and Mother Love, Courtship, and ... the reader of this work study its pages carefully and be able to give safe counsel and advice to others, and remember that purity of purpose and purity of character are the brightest jewels in...
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Searchlights on Health: Light on Dark Corners, by B.G. Jefferis and J. L. Nichols doc
... COMPLETE SEXUAL SCIENCE AND A Guide to Purity and Physical Manhood ADVICE TO MAIDEN, WIFE, AND MOTHER LOVE, COURTSHIP, AND MARRIAGE BY Prof B G JEFFERIS, M.D., PH.D., AND J L NICHOLS, A.M J L ... extent is daring, it is chaste, practical and to the point, and will be a boon and a blessing to thousands who consult its pages The world is full of ignorance, and the ignorant will always criticise, ... Light on Dark Corners A Complete Sexual Science and a Guide to Purity and Physical Manhood, Advice To Maiden, Wife, And Mother, Love, Courtship, And Marriage Author: B.G Jefferis J L Nichols Release...
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Báo cáo khoa học: Making sense of G-quadruplex and i-motif functions in oncogene promoters pot
... occurred It now appears that NM23H2 binds to both the purine and pyrimidine strands of NHE III1 but not to duplex [34], and the purported DNA strand cleavage [35,36] was due to a contaminating protein ... necessary for conversion of duplex DNA to G-quadruplexes and to identify proteins that might serve to facilitate the formation of and then resolve the G-quadruplex and i-motif structures in the nuclease ... wild-type and mutant sequence of the NHE III1 in the c-Myc promoter were inserted into a Del4 plasmid [16] A comparison of chemical (DMS, KMnO4 and Br2) and enzymatic (S1 nuclease, DNase 1) footprinting...
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assessment of the stress and strain levels at geometrical discontinuities under elastoplastic conditions a case study
... material, the actual stresses and strains were determined using the models of Neuber and Glinka and also using the simulations by finite element method for plane stress and plane strain states The ... another and could be satisfactorily characterized by two parameters: the notch root and the theoretical stress concentration factor Kt The availability of solutions for elastic stresses and strains ... the local stress and strain, being known the nominal stress and theoretical stress concentration factor It has been showed the energy density method can be used for plane stress and plane strain...
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biomechanics at micro- and nanoscale levels, v.iii, 2007, p.182
... method by which the structure and function of such cells and tissues can be regulated The first goal of this research is to understand dynamic phenomena at cellular and biopolymer-organelle levels ... to apply this understanding to the development of procedures for designing and producing artificial materials and technology for producing or regenerating the structure and function of living ... T Tateishi and T Ushida 118 IV COMPUTATIONAL BIOMECHANICS 131 MRI measurements and CFD analysis of hemodynamics in the aorta and the left ventricle M Nakamura, S Wada, S Yokosawa and T Yamaguchi...
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biomechanics at micro- and nanoscale levels, v.iv, 2007, p.181
... band was detected in the lane of WT prestin Two distinct bands of 100 kDa and 80 kDa were observed in the lane of G127A In the lanes of T128A, S129A and R130A, weak 100 kDa bands and 80 kDa and ... bands were recognized In the lanes of H131A and S129T, however, 80 kDa and 70 kDa bands were detected, but no 100 kDa bands were observed Based on our previous results [10], bands of 100 kDa and ... injury and a new constitutive model of skeletal muscle E Tanaka, D Ito, S Yamamoto and K Mizuno 141 Mechanical characteristics of vascular cells and tissues exposed to deformation, freezing and...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25