isolation of microsomes and cytosol from liver tissues
báo cáo khoa học: "Isolation of specific and biologically active peptides that bind cells from patients with acute myeloid leukemia (AML)" doc
... clones were then profiled with AML and normal cells Sixty-eight (15%) clones bound to CML-BC and AML cells, and were used for further studies with PB and BM cells from normal donors Of these 68 clones, ... and normal BM specimens in the presence of a single dose of 10-4 M peptide There was no significant decrease in the viability of cells cultured in the presence of any of the peptides tested and ... number of epitopes on the cells of the AML clone Blast analysis of the sequence of the two leukemia specific peptides revealed that of amino acids of HP-A2 matched mostly to proteins of lower...
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Tài liệu Báo cáo khoa học: Comparison of membrane fraction proteomic profiles of normal and cancerous human colorectal tissues with gel-assisted digestion and iTRAQ labeling mass spectrometry pptx
... Quantitative analysis of membrane proteins from paired tumoral and adjacent normal tissue of CRC patients A total of eight tumor tissues and eight matched normal tissues were collected from eight CRC ... membrane proteins from tumoral and adjacent normal tissues After surgery, paired tumoral and adjacent normal tissues were obtained from the same CRC patient and stored at )80 °C Frozen tissues were ... ª 2010 FEBS 3029 Profiles of membrane fractions from CRC patients J.-S Chen et al Purification of membrane proteins from adjacent non-tumor (N) and Tumor (T) tissues of CRC patients A-1 A-2 B-1...
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Tài liệu Báo cáo khoa học: Evidence for interactions between domains of TatA and TatB from mutagenesis of the TatABC subunits of the twin-arginine translocase docx
... domains and cytoplasmic regions of TatA and TatB, and the highly conserved residues of TatC have also been probed [24–27] In this report we have analyzed the transmembrane regions of TatA and TatB, ... 2005 FEBS Mutagenesis of TatABC structures of TM spans [32,33], usually introducing distortions of major proportions, and such substitutions were made in the TM spans of TatA and TatB in the present ... (2005) 2261–2275 ª 2005 FEBS C M L Barrett and C Robinson Mutagenesis of TatABC A B Fig Effects of mutations in the TM regions of TatA and TatB The TatA and TatB mutants containing alterations in...
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AKBAR, EMPEROR OF INDIA A PICTURE OF LIFE AND CUSTOMS FROM THE SIXTEENTH CENTURY ppt
... the lowlands and the slaughter of thousands of men, women and children of the predecessor's adherents; for five centuries northwest and northern India literally reeked with the blood of multitudes."[1] ... Akbar by deeds of partiality, selfishness and violence that in March 1560 Akbar, then 17 years of age, decided to take the reins of government into his own hand Deprived of his office and influence ... compilation and its commands folly, disputed the miracles of Mohammed and also the value of his prophecies, and denied the doctrine of recompense after death He professed the Brahman and Sûfistic doctrine...
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Báo cáo khoa học: Molecular characterization of artemin and ferritin from Artemia franciscana pot
... [24–27] Helices A and B are antiparallel, as are C and D, and they are connected by small loops A large loop, designated L, connects A and B helices with C and D helices, and L loops of neighboring ... heat and chemicals such as urea and guanidinium chloride [24], and the degradation of ferritin in vivo is inhibited by excess iron [32] To address questions of structure and function, artemin and ... with a labeled artemin probe revealed bands of 2.1 and 3.7 kb, the former of expected length based on the size of cloned cDNAs The mRNA in the upper band of the blot probed with the artemin cDNA...
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the geography of war and peace from death camps to diplomats oct 2004
... of Political Geography: World-Economy, Nation-State, and Locality, fourth edition (2000), and editor of Spaces of Hate (2004) Leila M Harris is assistant professor of geography and a member of ... at the University of Exeter and one of the world’s leading military historians He is editor of the journal Archives and a member of the Councils of the Royal Historical Society and the British ... Kenzer) Culture, Land, and Legacy (2003); and (with Michael K Steinberg and Joseph J Hobbs) Dangerous Harvests (2004) Alexander B Murphy is professor of geography at the University of Oregon, where...
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báo cáo sinh học:" Nurses'''' experiences of recruitment and migration from developing countries: a phenomenological approach" pdf
... obtain a sample of overseas nurses (ONs) i.e nurses who had migrated from other countries to Ireland Of the ten ONs, five were from India and five from the Philippines The mean age of the seven ... (Directors of Nursing) (six from Irish based teaching hospitals, three from South Africa and three from the Philippines), and 10 ONs The interviews lasted between 30 and 60 minutes and were semi-structured ... directors of nursing in both Ireland and the source countries, as well as the personal experiences and opinions of the overseas nurses themselves, currently working in Ireland The aim of this paper...
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Báo cáo khoa học: "Suppressive effect of culture supernatant of erythrocytes and serum from dogs infected with Babesia gibsoni on the morphological maturation of canine reticulocytes in vitro" pdf
... driht eht nO syad eerht rof yliad ecno god hcae fo niev lacivrec eht morf nekat saw doolb lm 042-002 tuobA desu erew gk 21-01 tuoba gnihgiew sgod yhtlaeh yllacinilc ruoF snoitacifidom emos htiw ... snegrevid B fo serutluc ortiv ni fo stnatanrepus ehT ]02,71[ ygolores rof stnegitna fo noitalosi dna yticinegonummi fo yduts eht rof desu saw murapiclaf muidomsalP fo tnatanrepus erutluc ortiv nI ... )s(rotcaf niatrec niatnoc thgim ti taht deredisnoc ew ,eroferehT tceffe evisserppus a dah inosbig B htiw detcefni sgod morf mures eht taht dnuof ew ,yduts roirp a fo esruoc eht nI ]6[ ortiv ni )PMI-'5(...
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Báo cáo khoa học: "Differentiating the impact of anatomic and non-anatomic liver resection on early recurrence in patients with Hepatocellular Carcinoma" pps
... points of the study were: 1- In-hospital mortality, morbidity and length of hospital stay 2- The detection of early disease recurrence through the year follow-up period The impact of the type of ... discoloration of the parenchyma after ligation of the corresponding arterial and portal venous branches or both Non-anatomic resection (n = 25) was defined as the resection of the tumor with a margin of ... subjected to a close followup of years During this period they underwent clinical, radiologic (abdominal US and triphasic abdominal CT scan) and biologic (serum AFP and liver function tests) evaluations...
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simultaneous removal of arsenic and ammonia from groundwater by phytofiltration with cattails (typha spp.) cultivation tt
... HANOI UNIVERSITY OF SCIENCE TECHNISCHE UNIVERSITAT DRESDEN NGUYEN TIEN CU SIMULTANEOUS REMOVAL OF ARSENIC AND AMMONIA FROM GROUNDWATER BY PHYTOFILTRATION WITH CATTAILS (TYPHA ... BY PHYTOFILTRATION WITH CATTAILS (TYPHA SPP.) CULTIVATION MASTER THESIS FIELD: WASTE MANAGEMENT AND CONTAMINATED SITE TREATMENT SUPERVISOR: ASSC DR LE VAN CAT HANOI-2008 MỤC LỤC MỤC LỤC DANH MỤC...
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simultaneous removal of arsenic and ammonia from groundwater by phytofiltration with cattails (typha spp.) cultivation
... role in phytofiltration systems because the type and textile of soil affect physical, chemical, and biological mechanisms regulating the removal of contaminants from water and soil Sandy soil, ... contaminants ) 1.4 Phytofiltration Systems 1.4.1 Characteristics of Phytofiltration Systems a/ Phytofiltration: Phytofiltration, the use of plant with extensive root systems and high accumulation ... of nitrogen is one of the main processes involved in the removal of ammonia from water Removal of nitrogen through plant uptake will depend on the growth rate of the plant, culture density, and...
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Báo cáo y học: "Characterization of erythrovirus B19 genomes isolated in liver tissues from patients with fulminant hepatitis and biliary atresia who underwent liver transplantation"
... the liver from patients who underwent liver transplantation In this retrospective study of 47 patients with liver diseases of unknown etiology, B19 DNA was detected in the liver obtained from ... from 1994 to 2000 Majority of patients had history of blood transfusion or blood product infusion in their course of treatment The liver tissues and serum samples from recipients obtained at ... genome In addition, we sequenced the B19 isolates consisted of 4561 bp covering NS1, VP1 and VP2 regions obtained from liver tissues of patients For this purpose, three overlapping PCR products...
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Báo cáo khoa học: "Optimization of the Agar-gel Method for Isolation of Migrating Ascaris suum Larvae From the Liver and Lungs of Pigs" pptx
... Fresh livers and lungs from an abattoir were used as supplements in Exps and (livers) and Exps 6, and (lungs) to increase the amount of tissue available for analysis Pieces of tissue obtained from ... Effect of incubation time, glucose and cooling Here, we tested the effect of incubation time of the liver agar-gels (3 or h), and addition of glucose to the saline plus the Table Impact of liver ... 1: Effect of blending time and gentamycin This experiment was designed to examine the rate of migration out of liver agargels (1, 2, and h), the effect of blending time and the effect of adding...
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Tài liệu Báo cáo khoa học: The isolation and characterization of cytochrome c nitrite reductase subunits (NrfA and NrfH) from Desulfovibrio desulfuricans ATCC 27774 Re-evaluation of the spectroscopic data and redox properties ppt
... intense band of 61 kDa (NrfA) and a band of weak intensity of 19 kDa (NrfH), confirming its hetero-oligomeric nature (Fig 1, lane 1) However, in the absence of boiling (Fig 1A, lanes and 4) high ... and 4) high molecular mass bands of approximately 110 kDa and > 200 kDa were visible, as well as a faint band at 37 kDa, suggesting the presence of dimers All of the bands stained positively for ... corresponds to 14% of the total iron absorption, and each NrfH heme corresponds to 7% From previous EPR studies [23], two sets of low-spin ferric g-values (gmax at 2.96 and 3.20) and a highspin heme...
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Preparative isolation and purification of six volatile compounds from essential oil of Curcuma wenyujin using high-performance centrifugal partition chromatography doc
... total of mg of curdione (1), mg of curcumol (2), 10 mg of germacrone (3), 18 mg of curzerene (4), mg of 1,8-cineole (5) and 17 mg of b-elemene (6) from the essential oil (300 mg) in 2.4 mL of the ... the differences between K2 and K3, and between K4 and K5 in solvent systems (petroleum ether/acetonitrile/acetone 2:1:1 and 4:3:1 v/v/v) are 0.34 and 0.24, and 0.20 and 0.20, respectively, which ... Both of the upper and the lower phases were degassed for 30 by ultrasonication before use Aliquots of 300 mg of the essential oil were diluted by 2.4 mL of the mixed solution of lower phase and...
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Báo cáo Y học: Cloning and expression of sterol D14-reductase from bovine liver potx
... beginning of incubation both in COS-7 cells and bovine liver microsomes Endogenous D14-SR activity of microsomes obtained from control COS-7 cells, measured on the basis of C27D8 formation and C27D8,14 ... long and contained an ORF of 1257 bp, encoding a protein of 418 amino acids with a calculated molecular mass of 46 751 Da The N-terminal amino-acid sequence of the protein puri®ed from liver and ... presence of signature patterns conserved from yeast D14-SR (ERG24 gene) and sterol D24(28)-reductase (ERG4 gene), as well as the degree of similarity with human LBR and D14-SR from plants and yeast,...
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Báo cáo khoa học: Isolation, characterization, sequencing and crystal structure of charybdin, a type 1 ribosome-inactivating protein from Charybdis maritima agg. potx
... characterization and structural analysis of charybdin, a novel 29-kDa type ribosome-inactivating protein, from bulbs of the white variety of C maritima agg Ribosome-inactivating protein from C maritima ... characterization and crystallization of charybdin The yield of the purified protein was 150–200 mg protein per 100 g of bulbs Charybdin appeared as a single band with a molecular mass of 29 kDa on ... additional two-stranded b-sheet (strands and 3, Figs and 5), which lies opposite the C-terminal domain This b-sheet is not well conserved among the known RIPs and is missing in the numbering of the structural...
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Báo cáo khoa học: Isolation and structural characterization of the Ndh complex from mesophyll and bundle sheath chloroplasts of Zea mays pptx
... types of plastids The Ndh complex associates in homodimers and dissociates in membrane and soluble subcomplexes; isolation of the Ndh complex by BN / PAGE and Tricine / PAGE Intact MS and BS ... exists as a monomer and dimer and splits into a membrane and soluble subcomplexes Separation of the Ndh complex by CN / PAGE (1D) and Tricine / PAGE (2D) and CN / PAGE (1D) and BN / PAGE (2D) ... GELGIYLVGDDSLFPWR and IRPPGFINLQILPQLVK (one missed cleavage) All of these peptides were part of the maize NdhH subunit Database analysis of a MALDI-TOF-MS measurement of a 21-kDa trypsin-digested gel band...
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Báo cáo Y học: Isolation, enzymatic properties, and mode of action of an exo-1,3-b-glucanase from Trichoderma viride doc
... exo-1,3-b-glucanase from T viride AZ36 Mode of action in the hydrolysis of laminarins The mode of action of the exo-1,3-b-glucanase was studied using laminarin from L digitata with a ratio of 1,3-b- and Exo-1,3-b-glucanase ... higher and the rate of hydrolysis is < twofold lower than for laminarin from L digitata The mode of action of the exo-1,3-b-glucanase on laminarin from L digitata was studied qualitatively by TLC and ... hydrolysis of 1,3-b-oligoglucosides, the subsite theory of Hiromi [46,47] was employed for construction of a subsite map for the estimation of affinities and number of subsites in the active center of...
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Báo cáo sinh học: " Application of FTA technology for sampling, recovery and molecular characterization of viral pathogens and virus-derived transgenes from plant tissues" doc
... sequences for TMV and the conserved region of CP and NiB from PVY and TEV potyviruses using specific primers (Table 1) PCR amplification of genomic DNA sequences from FTA cards Leaf tissues from transgenic, ... Kenya and Malawi fields in geminivirus DNA eluted from FTA-preserved leaf tissues of infected maize and cassava plants growing in farmer's Analysis of Analysis of geminivirus DNA eluted from FTA-preserved ... bring to sampling and molecular study of viral crop diseases, we tested the efficacy of FTA for retrieval of viral pathogens from infected leaf tissues and for the detection of viralderived transgene...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25