isolation of low n amyloid b protein oligomers from cultured cells csf and brain
Báo cáo khoa học: An E3 ubiquitin ligase, Synoviolin, is involved in the degradation of immature nicastrin, and regulates the production of amyloid b-protein doc
... degradation of < /b> NCT with time between synoviolin-null cells and wt cells As shown in Fig 2, western blot analysis of < /b> the intracellular degradation of < /b> NCT in synoviolin-null cells and wt cells following ... Supporting Information) We also determined the levels of < /b> c-secretase-unrelated ER protein (calnexin) and cytoskeleton protein (tubulin) in these cells as the internal control proteins The calnexin and ... mechanism underlying the enhancement of < /b> Ab generation by Synoviolin will clarify the interaction between the ubiqutination of < /b> the PS complex and APP processing Materials and methods Antibodies,...
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Báo cáo khoa học: b-Amyloid protein oligomers induced by metal ions and acid pH are distinct from those generated by slow spontaneous ageing at neutral pH docx
... inhibited by Ca2+ and Fe2+ and largely unaffected by Mg2+ Effect of < /b> pH and metal ions – CR binding and sedimentation analysis Because of < /b> the apparent discrepancy between our results using PAGE, which ... sedimentation assays but inhibited aggregation on PAGE, we examined the possibility that the aggregated Ab measured by the sedimentation assay and CR binding assay may be unstable and consequently not ... did not cause any significant loss of < /b> Ab as there was no significant change in the concentration of < /b> UV-absorbing material following filtration The peptide was stored at )80 °C Under these conditions,...
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Báo cáo Y học: Solution structure of the Alzheimer amyloid b-peptide (1–42) in an apolar microenvironment Similarity with a virus fusion domain potx
... The C-terminus of < /b> the beta protein is critical in amyloidogenesis Ann NY Acad Sci 695, 144–148 Selkoe, D.J (1994) Normal and abnormal biology of < /b> the b -amyloid < /b> precursor protein Annu Rev Neurosci ... Accordingly, it is possible that the mechanism of < /b> membrane interaction and destabilization is different in the case of < /b> Ab, Fig Comparison of < /b> the shapes of < /b> the lowest energy structure of < /b> HA_fd (A) and ... the solubility and spectroscopic features of < /b> Ab-(1–42) in a variety of < /b> media in different concentration and temperature conditions Several organic solvents and mixtures of < /b> organic solvents with...
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Tài liệu Báo cáo Y học: Toxicity of substrate-bound amyloid peptides on vascular smooth muscle cells is enhanced by homocysteine docx
... Purification and cloning of < /b> amyloid < /b> precursor protein b- secretase from human brain Nature 402, 537–540 Vassar, R., Bennett, B. D., Babu-Khan, S., Kahn, S., Mendiaz, E.A., Denis, P., Teplow, D .B. , Ross, ... E22Q and Iowa D2 3N < /b> mutations have been shown to be toxic to human leptomeningeal smooth muscle cells in culture [31,34–36] As binding of < /b> Ab to the basement membrane is an early step in Ab-induced ... effect was more prominent with Ab1–42 than Ab1–40 The inhibition of < /b> adhesion and subsequent toxicity to the cells by Ab may be important in cerebrovascular pathogenesis of < /b> amyloid < /b> angiopathy The greater...
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The Impact of Low Income on Child Health: Evidence from a Birth Cohort Study pptx
... evidence of < /b> an association between SES and health in adults (e.g the Black report (Townsend and Davidson 1982) and its follow up (Independent Inquiry into Inequalities 1998), and that this difference ... examine focus on low < /b> income early in life and examine the importance of < /b> different low-< /b> income sequences between 32 weeks gestation and 33 months (a total of < /b> four low-< /b> income observations) on poor ... 81 months We identify the importance of < /b> timing by comparing differences between low-< /b> income occurring at the start and the end of < /b> the low-< /b> income observation window, for a total of < /b> one, two and three...
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Báo cáo khoa học: Thermodynamic analysis of the unfolding and stability of the dimeric DNA-binding protein HU from the hyperthermophilic eubacterium Thermotoga maritima and its E34D mutant pdf
... Schindelin, H., Scholtz, A.S & Heinemann, U (1993) Conformations and conformational changes of < /b> four PhefiTrp variants of < /b> the DNA-binding histonelike protein, HBsu, from Bacillus subtilis studied by ... according to protein concentration: mm between and 14 lM, mm between 10 and 45 lM, and 0.2 mm between 30 and 180 lM For all the CD experiments, protein samples were prepared by diluting the same ... 3.50 and even at pH 3.25 (data not shown) fit the model well under the assumptions mentioned above, at pH 3.50 and below both protein variants are only marginally stable and thus their thermal unfolding...
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![Báo cáo khoa học: A dimer of the FeS cluster biosynthesis protein IscA from cyanobacteria binds a [2Fe2S] cluster between two protomers and transfers it to [2Fe2S] and [4Fe4S] apo proteins ppt](https://media.store123doc.com/images/document/14/rc/tl/small_tlm1396203606.jpg)
Báo cáo khoa học: A dimer of the FeS cluster biosynthesis protein IscA from cyanobacteria binds a [2Fe2S] cluster between two protomers and transfers it to [2Fe2S] and [4Fe4S] apo proteins ppt
... unknown reasons the extent of < /b> cluster binding by the C75A variant was found to be lower in all cluster insertion experiments In contrast, the extent of < /b> cluster formation in the variants C34A and ... coordination of < /b> the iron sites and the magnetic behaviour strongly indicate the presence of < /b> some nonprotein bound (superparamagnetic) iron(III) aggregates that precipated during iron incubation We ... Watanabe, A (1996) Sequence analysis of < /b> the genome of < /b> the unicellular cyanobacterium Synechocystis sp strain PCC6803 II Sequence determination of < /b> the entire genome and assignment of < /b> potential protein- coding...
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Báo cáo Y học: The amyloid precursor protein interacts with neutral lipids Liposomes and monolayer studies pdf
... free protein indicates a low < /b> incorporation efficiency of < /b> APP during the reconstitution procedure The influence of < /b> lipid /protein ratio on efficiency of < /b> protein incorporation into liposomes during reconstitution ... microgram quantities of < /b> protein utilizing the principle of < /b> protein- dye binding Anal Biochem 72, 248–254 32 Angrand, M., Briolay, A., Ronzon, F & Roux, B (1997) Detergent-mediated reconstitution of < /b> a ... The specific protein concentration used induced no change in surface tension of < /b> the buffer–air interface during h, after which the protein was injected into the subphase in the absence of < /b> preformed...
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Báo cáo y học: "Serum levels of autoantibodies against C-reactive protein correlate with renal disease activity and response to therapy in lupus nephritis" pps
... anti-C-reactive protein antibody levels in responders and nonresponders to given therapy At baseline, there was a highly significant difference in anti-C-reactive protein (anti-CRP) antibody levels between ... can therefore not be done ad infinitum Laboratory variables such as circulating complement components and anti-dsDNA antibodies can be helpful, but there is an urgent need for additional reliable ... studies, we refrain from the use of < /b> BSA to block nonspecific IgG-binding in our anti-CRP antibody assay, since serum antibodies to BSA (and other dietary proteins) are common and may thus affect...
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Báo cáo khoa học: Heteromer formation of a long-chain prenyl diphosphate synthase from fission yeast Dps1 and budding yeast pptx
... obtained from TOYOBO (Osaka, Japan) and New England Biolabs Japan (Tokyo, Japan) Protein markers were obtained from Fermentas Life Sciences (Ontario, Canada) and Oriental Yeast (Tokyo, Japan) Antibodies ... Whatman (Maidstone, UK) The Blue Native-PAGE NOVEX Bis-Tris Gel System and the NativeMark Unstained Protein Standard were obtained from Invitrogen (Osaka, Japan) Strains and plasmids The strains and ... formation of < /b> Dps1 and Coq1 A275 nm E F G A275 nm A275 nm D A275 nm C A275 nm B A275 nm A A275 nm M Zhang et al Fig Complementation and ubiquinone (UQ) extraction of < /b> an E coli ispB disruptant by the...
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Báo cáo toán học: "Dense Packings of Equal Disks in an Equilateral Triangle: From 22 to 34 and Beyond" pptx
... packings by suffixing their labels with the number of < /b> bonds Other examples are t17a40, t17a42, and t17a43 in Fig 2.1, t2 2b4 2 and t2 2b5 0 in Fig 3.1 However, even the number of < /b> bonds may not distinguish ... diameter and the same number of < /b> bonds (e.g., as in t1 7b4 2ns and t1 7b4 2s in Fig 2.1) Conjectures for infinite classes Dense packings in an equilateral triangle seem to “prefer” to form blocks of < /b> dense ... n=< /b> ∆(k+1)-1 • 119 120 • n=< /b> ∆(k) 120 n,< /b> number of < /b> disks packed Figure 6.1: Discrepancy between side length of < /b> the triangle and its lower bound for different n < /b> 34 the electronic journal of < /b> combinatorics...
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Báo cáo y học: "Global impression of perceived difficulties in children and adolescents with attention-deficit/hyperactivity disorder: Reliability and validity of a new instrument assessing perceived difficulties from a patient, parent and physician persp
... coefficients indicate a moderate agreement between parents' and physicians' ratings However, the agreement between parents and physicians on the one hand and the patients on the other hand were ... days and nights of < /b> your patient been generally? Please include all information provided by the patient and information you may have been able to obtain from other persons who know your patient ... agreement in the ratings of < /b> parents and their children For example, Achenbach et al [48] found in their meta-analysis a correlation of < /b> r = 0.25 between parents and children ratings of < /b> behavioral and...
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Tài liệu Báo cáo khoa học: Glycation of low-density lipoprotein results in the time-dependent accumulation of cholesteryl esters and apolipoprotein B-100 protein in primary human monocyte-derived macrophages docx
... some of < /b> these materials (e.g Ne-carboxymethyl-lysines and Ne-carboxyethyl-lysines and pentosidine) being known to accumulate with age on tissue proteins, and at an increased rate in LDL and atherosclerotic ... lipid and protein has been characterized, as well as the subsequent turnover of < /b> the apoB protein It is shown that both lipid and protein are taken up, in a time-dependent manner, via scavenger ... (1994) Binding and modification of < /b> proteins by methylglyoxal under physiological conditions A kinetic and mechanistic study with Nalpha-acetylarginine, Nalpha-acetylcysteine, and Nalpha-acetyllysine,...
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Tài liệu Báo cáo Y học: Proteolysis of bovine b-lactoglobulin during thermal treatment in subdenaturing conditions highlights some structural features of the temperature-modified protein and yields fragments with low immunoreactivity pptx
... fragments include a generous portion of < /b> the b- barrel structure (strands B, C and part of < /b> strand D), along with part of < /b> the distant I strand in the C-terminus region that includes Cys160 and connects ... point between monomers in the BLG dimer There are a number of < /b> interprotein hydrogen bonds in this region, in addition to hydrophobic interactions (such as those between Ile147 and Leu149 and the corresponding ... could be modified without sensible modification in the remainder of < /b> the structure [36] On the other hand, treatment in subdenaturing conditions has revealed transient formation of < /b> a number of < /b> unfolding...
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Báo cáo khoa học: Identification and expression of the first nonmammalian amyloid-b precursor-like protein APLP2 in the amphibian Xenopus laevis ppt
... Amino acid sequence comparison between the Xenopus and mammalian APLP2 proteins (A) Alignment of < /b> the amino acid sequences of < /b> Xenopus APLP2-A /B, and human, mouse and rat APLP2 proteins The one ... Coyle, J.T (1988) Neuroanatomical localization and quantification of < /b> amyloid < /b> precursor protein mRNA by in situ hybridization in the brains of < /b> normal, aneuploid, and lesioned mice Proc Natl Acad Sci ... expressed in Xenopus brain, intestine, ovary and stomach The mRNA forms lacking exon 14 were more abundant in testis, liver, heart, spleen and kidney than in oocytes, lung and muscle, where an equal...
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Tài liệu Báo cáo khoa học: Animal models of amyloid-b-related pathologies in Alzheimer’s disease docx
... in studying APP processing in sporadic AD brain, and in understanding the mechanisms of < /b> Ab truncation and modification Most transgenic models produce mainly full-length Ab, but by manipulating ... plaques and CAA Neuropathology Promoter Transgene Model PDGF Contact Ratio brain Ab ⁄ CSF Ab CSF Ab Ratio brain Ab ⁄ plasma Ab Plasma Ab Brain Ab (pmolÆg)1) Age -of < /b> -onset (months) humans (Table 1) ... has been proposed that Ab exists in a state of < /b> dynamic equilibrium between the plasma and central nervous system (brain and CSF) [105] If so, the ratio of < /b> [Ab ]brain ⁄ [Ab]plasma or [Ab ]CSF ⁄ [Ab]plasma...
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Tài liệu Báo cáo khoa học: Perturbation of membranes by the amyloid b-peptide – a molecular dynamics study pptx
... knowledge of < /b> the most basic interactions between Ab and a model membrane can lead to a more complete understanding of < /b> the membrane-aided assembly of < /b> Ab and the resulting damage to cell membranes ... This finding is independent of < /b> the protonation state and ionic strength of < /b> the surrounding aqueous medium, suggesting that electrostatic interactions and hydrogen-bonding are likely to be involved ... properties of < /b> lipid molecules surrounding the membrane-perturbing Ab40 peptide Although it is known that Ab can interact with the plasma membrane and assemble in this environment [6], a fundamental understanding...
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Tài liệu Báo cáo khoa học: A facile method for expression and purification of the Alzheimer’s disease-associated amyloid b-peptide pdf
... Ab(L1–40) is the dominant protein in the inclusion bodies In contrast, the major protein in the Ab(M1–42) inclusions was not Ab, but was the small heat shock protein IbpB (accession number B1 IYQ8), ... aggregation kinetics are typical of < /b> many nucleation-dependent polymerization processes, and have been documented in numerous studies on Ab, in which Ab42 has been shown to be more amyloidogenic than Ab40 ... of < /b> N-< /b> terminal methionine from recombinant proteins by engineered E coli methionine aminopeptidase Protein Sci 13, 1802–1810 25 Walsh DM, Lomakin A, Benedek GB, Condron MM & Teplow DB (1997) Amyloid...
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Tài liệu Báo cáo khoa học: Binding of N- and C-terminal anti-prion protein antibodies generates distinct phenotypes of cellular prion proteins (PrPC) obtained from human, sheep, cattle and mouse doc
... C-terminal binding antibody was used In contrast, a protein band at the size of < /b> the nonglycosylated full-length PrPC of < /b> humans, sheep and cattle was highly abundant when using C-terminal binding antibodies ... (C-terminal binding antibody) Fig Immunoblot analysis and diagrammatic presentation of < /b> PrPC bands obtained from three different regions of < /b> sheep brains (A) Immunodetection of < /b> PrPC derived from cortex ... PrPC banding patterns of < /b> various species detected by amino- and carboxyl-binding antibodies After immunoblotting, PrPC proteins were detected using N-< /b> or C-terminal binding antibodies The signal...
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Báo cáo khoa học: Structure of amyloid b fragments in aqueous environments docx
... study, indicating the sequence dependence of < /b> amyloidogenicity in the case of < /b> Ab Tk-RNase HII1)212–Ab28)42 and Tk-RNase HII1)174– Ab28)42 increased rapidly in fluorescence intensity under ThT-binding ... of < /b> Tk-RNase HIIm n< /b> Ab10)24, the proteins are not aggregated efficiently, because of < /b> the absence of < /b> an Ab C-terminal region Conformation of < /b> Ab28)42 in aqueous environments Structural studies of < /b> ... Ab1)40 and Ab1)42 Both forms are capable of < /b> assembling into b- sheet fibrils However, Ab1)42 plays an important role in the pathogenesis of < /b> AD The formation of < /b> aggregates and neurotoxicity of < /b> Ab1)42...
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