isolation of enteric pathogens from fecal specimens
Báo cáo y học: "ISOLATION OF CHLAMYDIA PNEUMONIAE FROM SERUM SAMPLES OF THE PATIENTS WITH ACUTE CORONARY SYNDROME"
... efficiency of C pneumoniae DNA extraction Sufficient recovery of final PCR product has been also seen when protein A from Staph aureus has been used for isolation of C pneumoniae from whole serum ... diagnostically relevant levels of IgA Simultaneous detection of increased titers of IgG and IgA was documented only in patients Surprisingly, when DNA specimens extracted from 1.0 ml of serum aliquots were ... µl of DNA was added to reaction mixture containing 1x PCR buffer (Silex, Moscow, RF) containing 10 mM Tris-HCl, pH 8,3 , 2.5 mM MgCl2, 15 pmol of each primer, 200 µM of each of dNTPs and U of...
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Báo cáo khoa học: "Etiology and antimicrobial susceptibility of udder pathogens from cases of subclinical mastitis in dairy cows in Sweden" pps
... Distribution of Udder Pathogens At least one microbial species supposedly associated with mastitis was isolated from 350 (60%) of the 583 quarter milk samples From the majority of those quarter ... http://www.actavetscand.com/content/53/1/36 Page of Table Distribution of sampled herds per county/ies and overall distribution of dairy herds per county/ies in Sweden County Number of sampled herds Number of dairy herds (SJV; ... stages of lactation J Dairy Sci 2009, 92(7):3124-3133 Bengtsson B, Unnerstad HE, Ekman T, Artursson K, Nilsson-Ost M, Waller KP: Antimicrobial susceptibility of udder pathogens from cases of acute...
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Báo cáo khoa học: "Isolation of Aspergillus spp. from the respiratory tract in critically ill patients: risk factors, clinical presentation and outcome" potx
... The mean length of ICU stay before isolation of Aspergillus spp was 32.1 ± 21.4 days Previous use of fluconazole was recorded in eight of the 36 patients (22.2%) with isolation of Aspergillus ... was found in 4% of cases, but in none of the patients was a diagnosis of invasive aspergillosis made In one study [19], because of the lack of reliable diagnostic tools, up to 60% of patients with ... presence of clinical features of pneumonia and isolation of Aspergillus spp from respiratory secretions In contrast, antifungal treatment should not be initiated when Aspergillus spp are recovered from...
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Detection of bacteria and enteric viruses from river and estuarine sediment
... of Bacteroides spp., Cryptosporidium spp and Adenovirus were extracted directly from 0.5 g wet sediment samples using ISOIL for Beads Beating (Nippon Gene, Tokyo) Detection of pathogens and fecal ... for Beads Beating Quantitative detection of fecal coliforms from sediment Fecal coliforms in the sediment samples were recovered following the procedure of Craig et al (2002) with some modifications: ... Bacteroides (61%, 22/36) The high prevalence of these fecal indicators suggested that part of the fecal indicator bacteria that flew into the river from various contamination sources settle on...
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Báo cáo khoa học: Isolation and identification of antimicrobial components from the epidermal mucus of Atlantic cod (Gadus morhua) docx
... Antimicrobial components of cod mucus G Bergsson et al Fig Purification of an antimicrobial component from 12 mg protein ⁄ peptide extract, prepared from skin mucus of cod, by use of HPLC The antimicrobial ... Antimicrobial components of cod mucus A B Fig Purification of antimicrobial components from 20 mg protein ⁄ peptide extract, prepared from the skin mucus of cod, by use of HPLC and monitoring the ... 110, 823–831 14 Zasloff M (1987) Magainins, a class of antimicrobial peptides from Xenopus skin: isolation, characterization of two active forms, and partial cDNA sequence of a precursor Proc...
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Báo cáo Y học: Amphibian peptides that inhibit neuronal nitric oxide synthase The isolation of lesueurin from the skin secretion of the Australian Stony Creek Frog Litoria lesueuri docx
... three-dimensional structures of certain domains of nitric oxide synthase isoforms are known [53±57], we are not aware of the three-dimensional structure of the calmodulin binding sites of any isoform being reported ... spectrum) Data from Y + fragmentations give sequence information from the N-terminal end of the peptide (see schematic arrows below the spectrum) For a review of fragmentations of MH+ ions of peptides ... 82±101 of caveolin-1 in the form of glutathione S-transferase fusion proteins have been shown to attenuate the activities of all three isoforms of NOS [62] A novel 10-kDa protein inhibitor of nNOS,...
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Báo cáo sinh học: " Chromatography paper strip sampling of enteric adenoviruses type 40 and 41 positive stool specimens" pot
... grant from the Union Shipping and Trade Company Ltd., Sofia, Bulgaria and by a doctoral scholarship from the University of Leuven, Belgium The authors thank Annemie Debacker and Katleen Maris, from ... adenovirusdifferent temPolyacrylamide gel electrophoresis of the PCR products amplified from the DNA of the subgroup F adenovirus positive stool sample, extracted from the SDS/EDTA pretreated chromatography ... analysis of adenoviral DNA from EAds type 40/41 positive stool specimens is a feasible method for sample collection The described filter paper strips facilitate collection, transport and storage of...
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Báo cáo sinh học: " Application of FTA technology for sampling, recovery and molecular characterization of viral pathogens and virus-derived transgenes from plant tissues" doc
... retrieval of viral pathogens from infected plant tissues allows not only identification of the viral pathogens but also detailed molecular study of their genomes, generating increased understanding of ... fragment of the coat protein gene from the A genomic component of ACMV generated using primers JSP001 and JSP002 (Table 1) (c) amplification of the 1.07 kb C1 gene of the Egyptian strain of the ... FTA cards PCR amplification from serial dilutions of viral DNA elution from FTA cards Serial dilutions of plasmid DNA carrying known amounts of the B genomic component of East African cassava Cameroon...
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báo cáo hóa học:" Chromatography paper strip sampling of enteric adenoviruses type 40 and 41 positive stool specimens" pot
... grant from the Union Shipping and Trade Company Ltd., Sofia, Bulgaria and by a doctoral scholarship from the University of Leuven, Belgium The authors thank Annemie Debacker and Katleen Maris, from ... adenovirusdifferent temPolyacrylamide gel electrophoresis of the PCR products amplified from the DNA of the subgroup F adenovirus positive stool sample, extracted from the SDS/EDTA pretreated chromatography ... analysis of adenoviral DNA from EAds type 40/41 positive stool specimens is a feasible method for sample collection The described filter paper strips facilitate collection, transport and storage of...
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báo cáo hóa học:" Application of FTA technology for sampling, recovery and molecular characterization of viral pathogens and virus-derived transgenes from plant tissues" doc
... retrieval of viral pathogens from infected plant tissues allows not only identification of the viral pathogens but also detailed molecular study of their genomes, generating increased understanding of ... fragment of the coat protein gene from the A genomic component of ACMV generated using primers JSP001 and JSP002 (Table 1) (c) amplification of the 1.07 kb C1 gene of the Egyptian strain of the ... FTA cards PCR amplification from serial dilutions of viral DNA elution from FTA cards Serial dilutions of plasmid DNA carrying known amounts of the B genomic component of East African cassava Cameroon...
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Báo cáo khoa học: "Isolation of cholesterol-lowering lactic acid bacteria from human intestine for probiotic use" ppsx
... extract fatty acid from the bacterial cell-wall and identified with microbial identification system (MIDI, Inc., USA) Isolation of bacteria: One hundred strains of LAB were isolated from selective ... broth without bacterial culture Isolation of cholesterol-lowering lactic acid bacteria 393 Table Number of acid- and bile-tolerant lactic acid bacteria isolated from the human intestine for probiotic ... In vitro cholesterol-lowering test: Among isolated 100 strains of LAB, of Streptococcus strains, 11 of Lactobacillus strains and of Bifidobacterium strains were selected as provisional probiotic...
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Báo cáo khoa học: " Isolation and characterization of Streptococcus sp.from diseased flounder (Paralichthys olivaceus )in Jeju Island" ppt
... hcae rof ylno devresbo yltnetsisnoc erew selpmas lla ni snoitacifilpma evitisop cificepS noitcefni lacoccocotperts rof evitagen si elpmas enO giF ni nwohs sa rof pb 817 ta selpmas 81 dna rof pb ... enilav ,esapil rof snoitcaer evitagen dah setalosi llA nispyrtomyhc -α dna esatahpsohp enilakla rof snoitcaer evitisop dah fo setalosi 81 dna esadinimasoculg-β-lyteca -N dna esaretse rof snoitcaer ... ,nicimatnec ,enozarepofec ,nillicipma ,nillicinebrac ot evitisnes erew setalosi 81 , roF mirpohtemirt /elozaxohtemaflus dna niotnarufortin ,mirpohtemirt ot evitisnes erew setalosi eerht , roF elbaT ni...
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báo cáo khoa học: "Isolation of specific and biologically active peptides that bind cells from patients with acute myeloid leukemia (AML)" doc
... probability of a biologic response of AML patients to The probability of a biologic response of AML patients to each peptide Cells from different AML specimens were incubated in the presence of peptides ... Figure cells from a capable of AML but not normal cells Peptides3arepatient withincreasing the cloning efficiency of Peptides are capable of increasing the cloning efficiency of cells from a patient ... for AML and normal BM specimens in the presence of a single dose of 10-4 M peptide There was no significant decrease in the viability of cells cultured in the presence of any of the peptides tested...
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Báo cáo y học: "First isolation of two colistin-resistant emerging pathogens, Brevundimonas diminuta and Ochrobactrum anthropi, in a woman with cystic fibrosis: a case report" potx
... count of 18,380/mm3 with 82.8% polymorphonuclear cells (PMNs), a platelet count of 618,000/mm3, C-reactive protein (CRP) of 57 mg/litre, fibrinogen of 5.17 g/litre and whole blood glucose of mmol/litre ... consent was obtained from the patient for publication of this case report and any accompanying images A copy of the written consent is available for review by the Editor-in-Chief of this journal Competing ... patient received a course of colistin to treat a A xylosoxidans colonization 10 months before the onset of this pneumonia This may have contributed to the selection of these two colistin-resistant...
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Báo cáo y học: " Rapid isolation of mycoviral double-stranded RNA from Botrytis cinerea and Saccharomyces cerevisiae" pot
... curves of the bands were processed with the aid of specific MediaCybernetics, Gel-Pro Analyzer Version 6.0 software Molecular cloning of the 2.2 kpb dsRNA from B cinerea CCg378 The dsRNAs of B ... CCg378 may correspond to or be part of the genome of a partitivirus [16], whereas those of the THg324 and SUg275 strains may correspond to the genome of members of the Hypoviridae and Totiviridae ... nucleic acids obtained from the g wet weight of fungal cells Under these conditions, the amount of total dsRNA recovered from a minicolumn was approximately μg, since of the 10 μL obtained, only...
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Báo cáo y học: "Identification and isolation of Genotype-I Japanese Encephalitis virus from encephalitis patients" pot
... first time from CSF samples of JE patients in China Previously, partial sequences of JEV GI were detected in specimens of meningitis patients in Japan [14] and in JE patients in an outbreak of China ... isolated from mosquitoes collected in these areas [12,13] Molecular epidemiological study showed that all of the JEV isolates obtained either from mosquitoes or from clinical samples of human ... number of nucleotide substitutions per site Sequences from this study are in boldface Viruses were identified by using the nomenclature of virus strain/ GenBank number/country/year of isolation/ origin...
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Báo cáo y học: "Isolation of mixed subtypes of influenza A virus from a bald eagle (Haliaeetus leucocephalus)" pptx
... species [15] Most of the surveillance studies [3,4,7,8] have focused on the detection of AIVs from waterfowl and shorebirds while only a few reports are available on isolation of AIV from raptors ... the first report on isolation of AIV in a Bald eagle from the U.S The bald eagle in question was recovered from Gloucester County, Virginia in March, 2008 In general, samples from injured raptors ... Sequences of A/greenwinged teal/Ohio/430/1987(H1N1) (CY011040) and A/ mallard/Ohio/37/1986(H2N1) (CY021125) were homologous to those of the bald eagle isolate Sequences of all eight segments from...
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Báo cáo khoa học: "Optimization of the Agar-gel Method for Isolation of Migrating Ascaris suum Larvae From the Liver and Lungs of Pigs" pptx
... the saline of some of the samples to a final concentration of 1% in the agar-gels and the incubation saline About 500 ml of crushed ice was added to some of the jars after the removal of the agar-gels ... Effect of incubation time, glucose and cooling Here, we tested the effect of incubation time of the liver agar-gels (3 or h), and addition of glucose to the saline plus the Table Impact of liver ... Impact of liver blending time, incubation time of agar-gels, and addition of gentamycin on numbers of A suum larvae (mean±SD) recovered from liver tissue of pigs Every hour gels were transferred to...
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Báo cáo khoa học: "Investigations of Waterborne Pathogens in Eurasian Beaver (Castor fiber) from Telemark County, Southeast Norway" ppt
... 480 F Rosell et al Table The number of Eurasian beavers (Castor fiber) from Telemark County, Norway, analysed for the presence of the bacterias Campylobacter spp and Salmonella spp., ... limited problem and most of the human clinical cases (80-90%) are infected abroad (Bredal & Langeland 1993, Fossum et al 1996) In spite of this, a waterborne outbreak of human S typhimurium infection ... range of wild and domestic mammals and birds which may also act as carriers Salmonella spp have also been isolated from beavers in Germany and Russia (Romasov 1992) Faecal samples were collected from...
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Báo cáo khoa học: "Antimicrobial Susceptibility of Udder Pathogens Isolated from Dairy Herds in the West Littoral Region of Urugua" ppsx
... susceptibility of udder pathogens 37 Table In vitro susceptibility of 43 strains of Enterococcus sp obtained from clinical and sub-clinical bovine mastitis cases from the West Littoral Region of Uruguay ... treatment of bovine mastitis, because of a low resistance rate Table In vitro susceptibility of 33 strains of Streptococcus uberis obtained from clinical and sub-clinical bovine mastitis cases from ... susceptibility of udder pathogens in dairy herds in a region of Uruguay, Thesis, International Master of Science Programme, Faculty of Veterinary Medicine, Swedish University of Agricultural...
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