... statistical analysis of the data and edited the paper ZA, TN, and AQ helped design the study, coordinated assays of the bloodand sputum samples, and edited the paper All authors have read and approved ... limits of detection of the assay for some COPD patientsand for many of the controls Table shows values for within-subject mean coefficients of variation, between-subject coefficients of variation, ... interactions of smoking and disease state All patients with COPD had to have at least GOLD stage II-IV COPD and a 10 pack-year history of smoking We excluded patients with a history of COPD exacerbation...
... forming blood vessels from surrounding tissue, potentially by proliferation and migration of pericytes from existing blood vessels, and also from the bone marrow: In the perivascular space of islet ... at birth and resulted in dilated blood vessels, haemorrhages and oedema besides other effects like anaemia, thrombocythemia, enlarged and deformed hearts and reduced size of livers and kidneys ... vessels had a variable and mostly increased diameter and an increased permeability The irregularity ofblood vessels in tumours again correlated with a decrease of coverage ofblood vessels by pericytes...
... kit, protein A and phenol-chloroform extraction method – molecular size standards; 2, and 10 – PCR-positive serum from patient M; 3, and 11 – PCR-positive serum from patient P; 4, and 12 – PCR ... 16 S rRNA and omp1) using DNA extracted from human plasma by phenol-chloroform method – molecular size standards; 2, and – PCR positive serum from patient M.; and – PCR positive serum from patient ... polymerase (Syntol, Moscow, RF); and 5pmol of both forward and reverse primers and 3,5 pmol probe The real-time PCR run was 10 at 95°C, and 50 repeats of 20 sec at 95°C and 50 sec at 62°C All samples...
... types of plastids The Ndh complex associates in homodimers and dissociates in membrane and soluble subcomplexes; isolationof the Ndh complex by BN / PAGE and Tricine / PAGE Intact MS and BS ... exists as a monomer and dimer and splits into a membrane and soluble subcomplexes Separation of the Ndh complex by CN / PAGE (1D) and Tricine / PAGE (2D) and CN / PAGE (1D) and BN / PAGE (2D) ... GELGIYLVGDDSLFPWR and IRPPGFINLQILPQLVK (one missed cleavage) All of these peptides were part of the maize NdhH subunit Database analysis of a MALDI-TOF-MS measurement of a 21-kDa trypsin-digested gel band...
... describe the isolation, sequence determination, and activities of two bioactive peptides from the skin glands of the stony creek frog Litoria lesueuri We have not studied a member of the L lesueuri ... lm), and lyophilized This work conforms with the Code of Practice for the Care and Use of Animals for Scienti®c Purposes (1990) and the Prevention of Cruelty to Animals Act Fig HPLC separation of ... colouration ranging from yellow to brown, with a black head stripe from the snout to the tympanum [35] The animal ranges from 37 to 63 mm in length, and is often found in the vicinity of rocky streams...
... positive for FSP and negative for cytokeratin 20 in all the cultured and examined cells (Figure 3) Discussion Q-banding analysis of FBs from CD and non-CD subjects demonstrated a normal and stable ... growth patterns of the cultures and to compare the basic morphometric characteristics of FBs Methods PatientsFrom September 2006 to January 2008, 58 consecutive subjects undergoing EGDS and agreeing ... area, and their ratio, as index of complexity Statistical analysis Data were expressed as mean ± standard deviation (SD) or median and range A comparison of the morphometric data obtained from...
... Detection rates of CTCs in 84 blood samples from 48 epithelial cancer patientsand in 30 samples from 22 melanoma patients Carcinoma Number ofblood samples Number ofpatients Positivity ofblood samples ... patients, and in 53% (17/32) sample frompatients with melanoma The median number of CTCs was (range: 1-55)/10 mL blood in epithelial cancer patientsand (range: 1-551)/10 mL blood in melanoma patients ... medians of CTCs of epithelial cancer patientsand melanoma patients P < 0.05 was considered significantly different Results Performance of three different enrichment methods Purity and recovery of...
... Detection rates of CTCs in 84 blood samples from 48 epithelial cancer patientsand in 30 samples from 22 melanoma patients Carcinoma Number ofblood samples Number ofpatients Positivity ofblood samples ... patients, and in 53% (17/32) sample frompatients with melanoma The median number of CTCs was (range: 1-55)/10 mL blood in epithelial cancer patientsand (range: 1-551)/10 mL blood in melanoma patients ... medians of CTCs of epithelial cancer patientsand melanoma patients P < 0.05 was considered significantly different Results Performance of three different enrichment methods Purity and recovery of...
... from the bacterial cell-wall and identified with microbial identification system (MIDI, Inc., USA) Isolationof bacteria: One hundred strains of LAB were isolated from selective (TATAC, LBS and ... strains of LAB, of Streptococcus strains, 11 of Lactobacillus strains andof Bifidobacterium strains were selected as provisional probiotic strains due to their superiority on low pH and bile ... Streptococcus and Lactobacillus) and pH 3.0 (for Bifidobacterium), respectively, by using N HCl, and put into ml per a ml vial Streptococcus and Lactobacillus were inoculated into MRS broth (pH 2.5) and...
... probability of a biologic response of AML patients to The probability of a biologic response of AML patients to each peptide Cells from different AML specimens were incubated in the presence of peptides ... with BM mononuclear cells from CML-BC patients After five rounds of biopanning, 450 individual phage were picked randomly and tested by ELISA against cells from CML-BC patients to confirm the phage ... clones were then profiled with AML and normal cells Sixty-eight (15%) clones bound to CML-BC and AML cells, and were used for further studies with PB and BM cells from normal donors Of these 68 clones,...
... curves of the bands were processed with the aid of specific MediaCybernetics, Gel-Pro Analyzer Version 6.0 software Molecular cloning of the 2.2 kpb dsRNA from B cinerea CCg378 The dsRNAs of B ... profile of the CCg378 strain revealed the presence of four dsRNA bands with approximate molecular sizes of 2.2, 1.95, 1.75 and 1.4 kilobase pairs (kbp) (Figure 2, lane 1), whereas the THg324 and ... isolationof mycoviral doublestranded RNA from Botrytis cinerea and Saccharomyces cerevisiae Virology Journal 2011 8:38 Submit your next manuscript to BioMed Central and take full advantage of: ...
... first time from CSF samples of JE patients in China Previously, partial sequences of JEV GI were detected in specimens of meningitis patients in Japan [14] and in JE patients in an outbreak of China ... HYW and QT participated in the serological studies, virus isolation, and the design of the study GDL conceived of the study, and participated in its design and coordination All authors read and ... careful review of the manuscript and useful suggestions This work was supported by grants from the Ministry of Science and Technology, People’s Republic of China (no.2003BA712A08-01 and 2009ZX10004-202);...
... gene of H1N1 was found to be more closely related to A/sanderling/DE/ 1258/1986(H6N6) The nucleotides of HA and NA genes of H2N1 subtype had 99% and 100% identity, respectively, with those of A/mallard/Ohio/37/1986 ... species [15] Most of the surveillance studies [3,4,7,8] have focused on the detection of AIVs from waterfowl and shorebirds while only a few reports are available on isolationof AIV from raptors ... Institutes of Health; rRT- Authors’ contributions PTR was responsible for collection of samples from bald eagles SMG and SS were responsible for overall coordination and planning of the study...
... of HBV A DL2000 10 11 281bp 132bp 109bp Counts of samples B B C D Genotype of HBV Figure of HBV reactive blood donors Genotyping Genotyping of HBV reactive blood donors All positive samples from ... experiments); B, sample counts of each genotype according A Figure Phylogenetic analysis of HBV strains from the blood donors Phylogenetic analysis of HBV strains from the blood donors HBV genome sequencing ... early and late stages of HBV infection: analyses of the window period and kinetics of HBV DNA Vox Sang 2005, 88:77-86 Sato S, Ohhashi W, Ihara H, Sakaya S, Kato T, Ikeda H: Efficacy of HBV NAT of...
... 1: Effect of blending time and gentamycin This experiment was designed to examine the rate of migration out of liver agargels (1, 2, and h), the effect of blending time and the effect of adding ... Acta vet scand vol 42 no 2, 2001 Isolationof migrating Ascaris suum larvae 281 Table Impact of incubation time of agar-gels, sedimentation time in incubation jars, and addition of ice and 1% glucose ... livers and lungs from an abattoir were used as supplements in Exps and (livers) and Exps 6, and (lungs) to increase the amount of tissue available for analysis Pieces of tissue obtained from the...
... before isolationof Aspergillus spp was 32.1 ± 21.4 days Previous use of fluconazole was recorded in eight of the 36 patients (22.2%) with isolationof Aspergillus spp., and in 41 of the 105 patients ... comparison of categorical variables A comparison of risk factors for the isolationof Aspergillus spp between groups ofpatients with Aspergillus spp., patients with Candida spp infection, and noncolonized, ... definitions of severe sepsis and septic shock used were those of the American College of Chest Physicians/Society of Critical Care Medicine Consensus Conference [9] In all patients, samples obtained from...
... be derived from subsequent isolationand re-plating of the ICM cells, which are then screened for stemness by a variety of recognized markers Once in stable culture, ESCs are capable of either ... originated from the intact/source embryo group and the single blastomere culture group were disaggregated from their respective blastocysts and re-plated on to fresh feeder cells for confirmation and ... scientifically sound, legal, and ethical, the NIH will examine the derivation of all existing stem cell lines and create Page of (page number not for citation purposes) Theoretical Biology and Medical Modelling...
... colonies on blood agar; hydrolysis of gelatin; production of lecithinase; and a positive reverse CAMP test RESULTS AND DISCUSSION result Also, H2S production properties of 3.1 Prevalence of C perfringens ... Genotyping of Clostridium perfringens isolated from cattle and pigs with diarrhea in Hanoi and surrounding areas, Vietnam 3.2 Genotyping of C perfringens the toxicity of C perfringens isolates and the ... Typing of isolates of Clostridium perfringens from healthy and diseased sheep by multiplex PCR Vet Med - Czech 50, 439-442 631 Genotyping of Clostridium perfringens isolated from cattle and pigs...
... elution using ml of methanol, 10 µL of the methanol eluent was added to 10 ml of CB medium (Watanabe and Satake, 1991) in a test tube, and after that M aeruginosa - 56 - Journal of Water and Environment ... (Oryza satives) seedlings by water extracts of C rotundus and leachate of its leaves, and found the existence of phenolic compounds such as 4-hydroxy benzoic and 3,4-dihydroxy benzoic acids Additionally, ... 500 Figure Comparison of fragment pattern of the peak E (a) at 13.508 with that of vanillic acid-TMS ester (b) and fragment pattern of the peak F (c) at 14.513 with that of protocatechuic acid-TMS...
... composition of silt and clay The low recovery rate was also because of the inhibition of nucleic acids extraction and PCR by humic substances extracted from sediment by high pH as Tsai and Olson ... (1996) and Watson and Blackwell (2000) reported The color of supernatant after vortex mixing and centrifugation was brown and that of the concentrate after membrane filtration was yellow, both of ... directly from 0.5 g wet sediment samples using ISOIL for Beads Beating (Nippon Gene, Tokyo) Detection of pathogens and fecal indicators by PCR and qPCR methods The concentration of (c)DNA of Poliovirus...