isolation expansion and labeling of mscs

Báo cáo khoa học: "SenseClusters: Unsupervised Clustering and Labeling of Similar Contexts" potx

Ngày tải lên : 08/03/2014, 04:22

... names or newgroups, and the number of contexts associated with each The next column indicates the percentage of the majority class (MAJ.) and count (N) of the total number of contexts for the ... Bisections, which offers nearly the quality of agglomerative clustering at the speed of partitional clustering Labeling Methodology For each discovered cluster, we create a descriptive and a discriminating ... names and uncategorized email Table shows the descriptive and discriminating labels assigned to the 2–way experimental case of American Airlines and Tom Cruise, as well as the 3–way case of George...

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Agricultural Biotechnology and Organic Agriculture: National Organic Standards and Labeling of GM Products pot

Ngày tải lên : 28/03/2014, 20:20

... Organic Standards and GM Product Labeling AgBioForum, 9(2), 2006 | 93 The market and welfare effects of labeling are shown to depend on the size of segregation costs under mandatory labeling of GMPs, ... Agricultural Biotech and Organic Agriculture: National Organic Standards and GM Product Labeling AgBioForum, 9(2), 2006 | 91 Figure The effects of mandatory labeling of GM products on organic and conventional ... Agricultural Biotech and Organic Agriculture: National Organic Standards and GM Product Labeling AgBioForum, 9(2), 2006 | 92 Table Market and consumer welfare effects of mandatory labeling of GM products...

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Báo cáo khoa học: "Unsupervised Discrimination and Labeling of Ambiguous Names" ppt

Ngày tải lên : 31/03/2014, 03:20

... Clinton and Tony Blair (political leaders), and Mexico and India (countries) We also have inter-category disambiguation such as Bayer, Bank of America, and John Grisham (two companies and an author) ... harmonic mean of precision and recall Precision is the percentage of contexts clustered correctly out of those that were attempted Recall is the percentage of contexts clustered correctly out of the ... ambiguity: 2-way, 3-way, and 4-way In each of the three categories we have 3-4 examples that represent a variety of different degrees of ambiguity We have created several examples of intra-category...

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Packaging and labeling of apparel and tetiles

Ngày tải lên : 30/07/2015, 10:34

... •Union labeling Registered number system •PERMANENT CARE LABELING RULE(1972) Voluntary labeling A Brand labeling  the labeling of fabrics with brand name is well established method and is used ... umbrellas and parasol  dish clothes, dishtowels and wash clothes •• TEXTILE FIBER PRODUCT IDENTIFICATION SILK REGULATION 1932 Voluntary labeling LABELING CATEGORIES VOLUNTARY LABELING •Brand labeling- ... presence of steam      CARE LABELING SYSTEMS International care labeling system Japan care labeling system British care labeling system Canadian care labeling system a International Care Labeling...

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Isolation, purification and detection of soyasaponins and their associated bioactivities in cultured hepatocarcinoma cells

Ngày tải lên : 11/09/2015, 10:06

... Structures and molecular weights of soyasapogenol B, C and B1 98 Fig 28 LC-MS Chromatogram of standard and purified sample of soyasapogenol B Panels (A and C) are authentic standard and samples of ... 79 6.2.1.5 HPLC Analysis of Soyasaponins and Soy isoflavones 79 6.2.1.6 LC/MS Analysis of Total soyasaponins and Soy isoflavones 79 6.2.2 Comparison of Soyasaponin Concentration Methods ... recovery of soyasapogenols A and B without producing artifacts (Ireland and Dziedzic 1986; Rupasinghe, Jackson et al 2003) Moreover, Ireland and Dziedzic (Ireland and Dziedzic 1986) showed that...

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Isolation, cloning and characterization of the FSH beta gene promoter of the chinook salmon (oncorhynchus tshawytscha)

Ngày tải lên : 08/11/2015, 17:15

... surface of the pituitary gonadotrope This leads to the synthesis and secretion of LH and FSH which stimulate the production of steroid hormones in their gonads Testosterone (T), estrogen (E) and ... stimulatory effects of GnRH and activin during puberty and sexual maturation 1.5 Aim The aim of this project is to initiate research on the mechanism of transcriptional regulation of the FSHβ subunit ... responsiveness of the promoter and the sequence will be analyzed to identify putative regulatory elements that may mediate these effects 23 MATERIALS AND METHODS 2.1 Extraction and isolation of genomic...

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Isolation, cloning and expression of NAD+ glycohydrolase from neurospora crassa

Ngày tải lên : 08/11/2015, 17:15

... Culture 2.2.2 Conidia Culture 32 33 ii CONTENTS 2.3 PAGE Isolation of N crassa Proteins 2.3.1 Isolation of Mycelia Proteins 2.3.2 Isolation of Conidia Proteins 33 33 Column Chromatography 2.4.1 ... Cycling Assay 81 Discussions 4.1 Isolation and Characterization of Conidial NADase from N crassa 83 4.2 Identification of NADase Sequence 85 4.3 Cloning and Expression of Gi|85106032 86 4.4 NAD+ Cycling ... LIST OF FIGURES FIGURE TITLE PAGE 1.1 De novo synthesis and salvage pathway of NAD+ 1.2 Reaction mechanism of NADase and ADP-ribosyl cyclase 1.3 Cycling assay for cADPR 15 1.4 Asexual cycle of...

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Isolation and characterization of an extracellular polymer closely related to flocculation of activated sludge

Ngày tải lên : 05/09/2013, 09:08

... polysaccharide One milligram of the partially purified polymer contained 309.7 µg of protein, 160.2 µg of neutral sugar, 58.5 µg of hexosamine, 30.8 µg of uronic acid and 3.8 µg of phosphorus Constituent ... concentration of ppm to estimate the effect of pH After min, A660 was measured and the extent of flocculation was calculated Pronase digestion of extracellular polymer Forty milligrams of the extracellular ... as follows: 500 g of soil was washed times with distilled water and suspended in 1,000 ml of water The mixture was allowed to stand for 10min and the supernatant was collected and used for the...

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Expansion and its realization in the short story “the law of life” by jack london

Ngày tải lên : 29/01/2014, 14:44

... understand them better Therefore, I would like to analyze a randomly chosen text in terms of expansion to see how the findings can help to understand some features of the text and the intention of ... interpersonal and the textual Each of these three metafunctions is about a different aspect of the world, and is concerned with a different mode of meaning of clauses Each of these three kinds of meaning, ... combination of and and another conjunctive element e.g and then, and afterwards, and at that time etc Halliday distinguishes four subtypes of enhancement: temporal, spatial, manner and causal-conditional...

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Tài liệu Báo cáo khoa học: Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds docx

Ngày tải lên : 18/02/2014, 16:20

... for cDNA single strand synthesis, 600 ng of RNA, 0.5 lg of oligo(dT) primer and 10 mm of dNTPs were incubated for at 65 °C Subsequently, lL of the ﬁrst strand buffer X and lL of dithiothreitol ... mixture of other isoforms (B) Gel visualization of proteins eluted Lane 1, molecular weight markers Numbers on the left indicate the Mr values of the standards in thousands Lanes 2–9, lg of peak ... P III ⁄ P IV and a mixture of other isoforms (*), respectively, in the presence (lanes 2–5) or absence (lanes 6–9) of 2-mercaptoethanol (C) Elution proﬁle of P III and P IV Chromatofocusing chromatography...

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Tài liệu Báo cáo khoa học: Phenol hydroxylase from Acinetobacter radioresistens S13 Isolation and characterization of the regulatory component docx

Ngày tải lên : 21/02/2014, 00:20

... of 50 nmÆmin)1, band-width of 1.0 nm, and a resolution of 0.1 nm in the temperature range between 10 °C and 70 °C, after preincubation for 10 at each temperature Three runs were accumulated and ... of 0.6 lM The data were fitted to Michaelis–Menten curves Squares and dotted line: data and fitting with PHI/PHO ratio of 0.5 Triangles and continuous line: data and fitting with PHI/PHO ratio of ... Asterisks and broken line: data and fitting with PHI/PHO ratio of hypothesis and therefore a direct involvement of PHI in electron transfer This conclusion is consistent with the very low degree of identity...

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Tài liệu Báo cáo khoa học: The isolation and characterization of cytochrome c nitrite reductase subunits (NrfA and NrfH) from Desulfovibrio desulfuricans ATCC 27774 Re-evaluation of the spectroscopic data and redox properties ppt

Ngày tải lên : 21/02/2014, 00:20

... intense band of 61 kDa (NrfA) and a band of weak intensity of 19 kDa (NrfH), conﬁrming its hetero-oligomeric nature (Fig 1, lane 1) However, in the absence of boiling (Fig 1A, lanes and 4) high ... and 4) high molecular mass bands of approximately 110 kDa and > 200 kDa were visible, as well as a faint band at 37 kDa, suggesting the presence of dimers All of the bands stained positively for ... between NrfH and NrfA is discussed The reassessment of previous spectroscopic studies was undertaken, particularly regarding the assignment of spectroscopic and redox potentials of the NrfA hemes...

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Preparative isolation and purification of six volatile compounds from essential oil of Curcuma wenyujin using high-performance centrifugal partition chromatography doc

Ngày tải lên : 07/03/2014, 21:20

... total of mg of curdione (1), mg of curcumol (2), 10 mg of germacrone (3), 18 mg of curzerene (4), mg of 1,8-cineole (5) and 17 mg of b-elemene (6) from the essential oil (300 mg) in 2.4 mL of the ... the differences between K2 and K3, and between K4 and K5 in solvent systems (petroleum ether/acetonitrile/acetone 2:1:1 and 4:3:1 v/v/v) are 0.34 and 0.24, and 0.20 and 0.20, respectively, which ... Both of the upper and the lower phases were degassed for 30 by ultrasonication before use Aliquots of 300 mg of the essential oil were diluted by 2.4 mL of the mixed solution of lower phase and...

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Báo cáo Y học: Isolation and characterization of a thioredoxin-dependent peroxidase from Chlamydomonas reinhardtii doc

Ngày tải lên : 08/03/2014, 16:20

... BLAST of peptide sequences of 21 kDa proteins in databases Tryptic peptides were puri®ed by RP-HPLC and some of them were totally or partially analyzed by Edman sequencing (ááá) and/ or by MALDI-TOF ... BAS1 protein of Brassica, spinach, barley and A thaliana, PR1 of Phaseolus and MHF of A thaliana These proteins belong to the 2Cys-Prx subfamily All plant 2Cys-Prx proteins, except BAS1 of barley, ... previously [21] Chloroplastic thioredoxins m and f from spinach were a kind gift of P Schurmann (University È of Neuchatel, Switzerland) Thioredoxins m and h from Ã Chlamydomonas were puri®ed as...

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Báo cáo khoa học: Isolation and characterization of a D-cysteine desulfhydrase protein from Arabidopsis thaliana pptx

Ngày tải lên : 16/03/2014, 18:20

... presequence of 19 amino acids The mature protein would have a molecular mass of 41.7 kDa and a pI of 6.34 The YedO protein from E coli and the d-CDes from Arabidopsis showed an overall identity of 36% and ... d-CDes mRNA and protein levels in older plants, during the light phase in a diurnal light ⁄ dark rhythm and under sulfate limitation Results In silico characterization and isolation of the Arabidopsis ... protein has a predicted molecular mass of 43.9 kDa and a pI of 7.2 It contains relatively high amounts of the sulfur amino acids cysteine (four residues) and methionine (10 residues) According...

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Báo cáo khoa học: Isolation, characterization and expression analysis of a hypoxia-responsive glucose transporter gene from the grass carp, Ctenopharyngodon idellus potx

Ngày tải lên : 17/03/2014, 03:20

... which contained 20 ng of first strand cDNA, · PCR buffer (20 mM Tris/HCl pH 8.4, 50 mM KCl), 0.2 lM of each primer, 1.5 mM MgCl2 and 0.2 mM of dNTPs PCR Construction and screening of a grass carp genomic ... KCl), lM of each primer, 0.2 mM of dNTPs, 1.5 mM MgCl2 and U of Taq DNA polymerase (Invitrogen) The PCR program consisted of predenaturation at 94 °C for min, followed by 35 cycles of amplification ... performed on first-strand cDNAs that were reverse transcribed from total RNA by use of Superscript II reverse transcriptase (Invitrogen) and consisted of 20 ng of first strand cDNA, · PCR buffer...

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Báo cáo khoa học: Isolation and characterization of an IgNAR variable domain speciﬁc for the human mitochondrial translocase receptor Tom70 potx

Ngày tải lên : 17/03/2014, 10:20

... respectively, and the resultant VNARs evaluated for retention of binding afﬁnity Material and methods Equipment and reagents Restriction enzymes and Vent DNA polymerase were purchased from New England ... possibility of inter- and intra CDR disulphide cross-links These strategies are summarized in Fig 1B and Table 1, and details of the library construction are given in greater detail in the Materials and ... possibility of disulphide bond formation genetically by replacement of residues Cys22 and Cys82 with alanine and valine, respectively, to give a cysteine minus mutant (12F-11DCys) Use of alanine and...

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FIELD STUDY OF ALLOPATRY, SYMPATRY, PARAPATRY, AND REPRODUCTIVE ISOLATION IN STEPPE BIRDS OF PATAGONIA pptx

Ngày tải lên : 22/03/2014, 12:20

... plored some of the questions of interest here, but attempt to combine an analysis of proximate becausehe worked west of the Andes and in the (ecological) and of ultimate (evolutionary) facAndes themselves, ... distribution lands and shrubsteppes of North America, enviof many Patagonian bird species,but are of little ronments that are equivalent to some of the use in elucidating patterns of overlap and reprosteppes ... West of Esquel toward the Chilean border, Andean valleys receive more and more rainfall, and have, first relatively dry and open Austrocedrus woodlands and then mesic to very humid beech (Nothofagus)...

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Báo cáo khoa học:The isolation and characterization of temperature-dependent ricin A chain molecules in Saccharomyces cerevisiae docx

Ngày tải lên : 23/03/2014, 07:20

... site molecules Y80, Y123 and E177 are shown in green and the position of the two mutated amino acids, F108 and L151, are shown in blue between the aromatic rings of Tyr80 and Tyr123 close to the ... diagram of wild-type RTA structure, and the positions of the altered amino acids of the double mutant, F108 and L151, within the structure are indicated Discussion RTA is the catalytic polypeptide of ... Utilisation of the budding yeast Saccharomyces cerevisiae for the generation and isolation of non-lethal ricin A chain variants Yeast 22, 1287–1297 16 Gemmill TR & Trimble RB (1999) Overview of N- and...

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Báo cáo Y học: Isolation, enzymatic properties, and mode of action of an exo-1,3-b-glucanase from Trichoderma viride doc

Ngày tải lên : 24/03/2014, 04:21

... hydrolysis of 1,3-b-oligoglucosides, the subsite theory of Hiromi [46,47] was employed for construction of a subsite map for the estimation of afﬁnities and number of subsites in the active center of ... Km and Vmax for hydrolysis of G6G are similar to those of G3G (Tables and 4) Therefore, it may be assumed that the enzyme has a mixed mode of action towards laminarins and that it is capable of ... purity of b-oligoglucosides was analyzed by TLC and 1H and 13C NMR spectroscopy as described below Published values for 1H and 13C chemical shifts of b-oligosaccharides with different DP and linkage...

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