... past I − (S) Similarly, one can define causal and chronological future J + (S) and I + (S), and thereby, in a standard way, the domain of influence and domain of dependence of an achronal set S ... hold with νRN and − µRN replaced by the ν and − µ of our initial data This follows from (32), (26), and the relation (36) ∂u (1 − µ) = −1 r ζ ν ν(1 − µ) − 2ν r2 e2 − r Indeed, (36) and (32) imply ... point of intersection of the right-I and the corresponding left-I region) and parametrize the u = line segment by ≤ v ≤ V with s = (0, 0) and p = (0, V ), and parametrization determined by the...
... GLUT1 and GLUT3 (exons 3–8), and four in human GLUT2 (exons 7–10) and GLUT4 (exons 6–9) (Table 1) The codons for arginine (96) and valine (231) in gcGLUT (Fig 2) are split between exons and 5, and ... (between exon and exon 5) and valine (between exons and exon 7) are shown Exonic regions are shown in uppercase and intronic regions are in lowercase The split codons are boxed and highlighted ... 5¢-CCTGATCGACGCACGAGT-3¢ and GT1-R, 5¢-TTTTGCAAGTCATAGTAATCAGTTT-3¢ for GTcDNA1 (2150 bp); and GT2-F, 5¢-CACCAGCAACTAC CTGATCGA-3¢ and GT2-R, 5¢-CACAAAATATGCTT CCAAGTGC-3¢ for GT-cDNA2 (3043 bp) RNA isolation and...
... Dr Tran Ngoc Ninh, Vietnamese Academy of Science and Technology Extraction andisolation Leaves of Clausena heptaphylla were dried at 40 ÷ 500C and powdered The powder (500 g) was extracted with ... ml of saline (for virus control and cell control) and given ml 2x MEM The cells were incubated for 72 hours and observed for cytopathic effects from the virus and cytotoxic effects from the samples ... MeOH 900 at 800C The extract was filtered and concentrated in vacuo to yield 84 g extract The concentrated extract was fractionated into Hexan, EtOAc, BuOH, and H2O-soluble fractions, sequentially...
... Glycosidase-Inhibiting Alkaloids: Isolation, Structure, and Application 111 Naoki Asano Introduction 111 Isolationand Structural Characterization 111 Deoxynojirimycin and Related Compounds 112 Isolation from Morus ... microbes and against desiccation; formation of a thick bark in roots and stems against water loss, microbes, and herbivores; development of spines, thorns, hooks, trichomes, and glandular and ... spp (Moraceae) 112 Isolation from Thai Medicinal Plants ‘‘Thopthaep’’ and ‘‘Cha Em Thai’’ 113 a-Homonojirimycin and Related Compounds 115 Isolation from Garden Plants 115 Isolation from the Thai...
... Gympie, Queensland outcrossed selfed Forestry and Forest Products Inbreeding problems Petford Vietnam landrace Petford Rwanda landrace E camaldulensis: natural seed source vs local land race Australian ... Isolation, inbreeding and relationships in forest trees Phenotype Selection (seed orchards, seed prod areas) Forestry and Forest Products Why is selection ... source vs local land race Australian seedlot (collected from natural stand, one year younger) Vietnam land race Forestry and Forest Products Open-pollinated eucalypt family insects are the main...
... [33,34], and identification of the convergent and divergent pathways between salinity and other abiotic stress responses and the nodes of signalling convergence may greatly enhance the understanding ... functioning [5], K+/Na+ selectivity [6] and Na+ exclusion and compartmentation [7-9], 4) cell signalling and gene expression, reflected as abscisic acid (ABA) and jasmonic acid (JA) accumulation ... enriched population of the overexpressed and newly induced transcript messages, TA1 and T2R were considered as 'Tester-A' and “Tester-B', respectively, and the C as the 'Driver' The opposite was...
... increasing our understanding of signalling and metabolic pathways underlying developmental and cellular processes Realtime RT-PCR is currently one of the more powerful and sensitive techniques ... genes were ranked based on their deviation from the mean (2× standard deviation) (Table 3) Highest ranked were SAND and actin in 2003 and 2004 pericarp, respectively, with minimal deviation around ... by actin, EF1-α, and SAND Due to seasonal differences observed using the different approaches (Figure 1; Tables and 3), further validation was initiated to test whether the top candidates continued...
... supported by the MRC Thanks to T Hernandez for help and J Brown and V Buckle for discussion and help I dedicate this work to my family for understanding and support, especially to my father 24 ... excluding volume forces and the interchromatin granule clusters become destabilized Understanding the mechanisms of compartmentalization is essential to understanding nuclear processes and their control ... with two very different dynamic regimes; chromatin on one hand, which shows constrained slow diffusion, and proteins and RNA on the other hand, which show diffusion at least three orders of magnitude...
... BOOM AND GLOOM The Credit and Banking Crisis of 2007–2009 and Beyond Violaine Cousin BANKING IN CHINA Vincenzo D’Apice and Giovanni Ferri FINANCIAL INSTABILITY Toolkit for Interpreting Boom and ... Hans Genberg and Cho-Hoi Hui THE BANKING SECTOR IN HONG KONG Competition, Efficiency, Performance and Risk Carlo Gola and Alessandro Roselli THE UK BANKING SYSTEM AND ITS REGULATORY AND SUPERVISORY ... regions, and studies of particular themes such as Corporate Banking, Risk Management, Mergers and Acquisitions, etc The books’ focus is on research and practice, and they include up-to-date and innovative...
... Structures and molecular weights of soyasapogenol B, C and B1 98 Fig 28 LC-MS Chromatogram of standard and purified sample of soyasapogenol B Panels (A and C) are authentic standard and samples ... recovery of soyasapogenols A and B without producing artifacts (Ireland and Dziedzic 1986; Rupasinghe, Jackson et al 2003) Moreover, Ireland and Dziedzic (Ireland and Dziedzic 1986) showed that ... et al 1982; Gurfinkel and Rao 2003; Wink 2008; Zhang and Popovich 2009) In this chapter, the isolation, chemical characterization and detection strategies focusing on HPLC and LC-MS to analyze...
... 16S-9F and 16S-1525R The result of PCR perform size band of strains and Bacillus subtilis control had 1500bp Sequencing perform that strains (Bs4 and BN5) were Bacillus subtilis (99% and 98% ... 45ml EtBr 0,8µl RESULTS AND DISCUSSION 3.1 Bacteria Isolationand characteristic: 3.1.1.Bacteria Isolation: 21 isolates bacteria strains isolates from “mud-dreg” took 42,85% and 12 from waste water ... reaction 2.2.8.Polymerase chainreaction 3.Result and Discussion 10 3.1.Bacteria Isolationand characteristic 10 3.1.1.Bacteria Isolation 10 3.1.2.Characteristic (size of cell,...
... (5’-GGTAGACCTTCTCGTAGGACCAC3’) and GSP3 (5’-GGTCATGTTGTTTAGTCGGCAGCCATACCT-3’) The PCR components and cycling parameters are in Table 20 and 21 except that the annealing temperature and extension time are 600C and 30s, ... putative cis-acting elements including a GSE, GnRH REs and several half androgen responsive elements (½ ARE) and EREs located contiguously between –187 and –124 bp upstream from a TATAA sequence, which ... receptors (ActRII) and two signal transducing type I receptors (ActRI; Massagué and Wotton, 2000) Upon ligand binding, the ActRII phosphorylates the ActRI on multiple serine and threonine residues...
... (Price and Pekala, 1987; Anderson, 1982) Mammalian NADases are generally found in association with the plasma membranes, therefore insoluble, and inhibited by nicotinamide (Pekala and Anderson, ... only below pH and above pH (Kaplan, 1951) In additional to mycelial NADase, NADase from conidia has been purified and characterized by Menegus and Pace (1981) They are the first and only one group ... transcriptional level (Roa and Blobel, 1983; Roggenkamp et al 1984) The promoter of AO was identified and isolated by Ellis and co-worker (1985) Following this, Cregg and co-worker (1985) has successfully...
... Water and Environment Technology, Vol.3, No.2, 2005 viscous in solution, are mainly composed of polysaccharides, although proteins and nucleic acids from cell autolysis may be present (Stoveland and ... kaolin clay and activated charcoal flocculated satisfactorily (the flocculation values at pH 2.5 of kaolin clay and activated charcoal were 79.6% and 67.1%, respectively) On the other hand, in the ... Significance and behavior of heavy metals in waste water treatment process Ⅰ Sewage treatment and effluent discharge Sci Total Environ., Vol.30, 1-44 Lowry, O Hosenbrough, N J., Farr, A L and Randall,...
... populations in Lakes Ohnuma and Akanko (sites 29 and 30) were thought to have been introduced from the island of Honshu in Japan (Ban and Minoda 1989) Morphological Stasis and Plasticity For the copepod ... genetic distance and reproductive isolation among species of Drosophila (Coyne and Orr 1989, 1997) and among populations of the splash pool copepod Tigriopus californicus (Edmands 1999) Levels ... Bothnia; (33) Gulf of Finland; (34) Sallvik Fjord, Finland; ¨ (35) Baltic Sea Proper; (36) IJsselmeer, Netherlands; (37) Gironde estuary, France; (38) Tamar estuary, England ence among the non-European...
... SJS133 and SJS134 as the external primers and the constructs pTETGLOPAI)2 and pTETGLOARE II-MUT as the templates Mutation of the ARE I-AUUUA and ARE III-AUUUA sequences used primers SJS172 and SJS173, ... SJS173, and SJS174 and SJS175, respectively Mutation of the atypical AU-rich sequence used primers SJS259 and SJS260, and the creation of the ARE II ⁄ ARE IV double mutant used primers SJS261 and ... the contribution and mechanism of this ARE, and the individual ARE domains, to PAI-2 mRNA stabilization and, subsequently, PAI-2 gene expression Experimental procedures Plasmids and mutant construction...
... wholecell extracts and the supernatant and precipitate fractions are shown in Fig It was found that E coli BL21(DE3) ⁄ pHYDJs and E coli BL21(DE3) ⁄ pHYDPf produced a predominant band with an apparent ... purified proteins; M, molecular weight standards For the molecular weight standards, the bands from top to bottom correspond to 116.0, 66.2, 45.0, 35.0, 25.0 and 18.4 kDa, respectively consistent ... SGL2 and SGL3, correspond to residues 60–71, 91–99 and 151–161, respectively On the basis of the homology model, the SGLs of HYDJs and HYDBp (PDB accession number 1NFG) were superimposed and compared...
... (types A+, B+ and O+), rabbit and horse where they showed blood group specificity and distinct hemagglutination activity P I and P II promoted hemagglutination of human erythrocytes at 22.5 and 27.5 ... isolated, and their amino acids sequences deduced by cDNA cloning and verified by MS Half maximal inhibitory concentration (IC50) and median lethal dose (LD50) values from HeLa cells and mice divided ... numerals I, II, III and IV and refers to each pulchellin Characterization of four pulchellin isoforms isoform (P I, P II, P II and P IV) The A-chain of P II was formerly cloned and named recombinant...
... these cells Mesenteric BECs and LECs (Fig 5A,B) were also obtained by the same strategy as illustrated in Figs and 4, and were treated with VEGF-A or VEGF-C (Fig 5C) MAPK and Akt phosphorylation ... induced in both BECs and LECs by stimulation with VEGF-A or VEGF-C, indicating that the cultured ECs responded to the endothelial growth factors Isolationand characterization of BECs and LECs We next ... introduced by lipofection and maintained for at least days after transfection as assessed by GFP expression (Fig 4E) These Fig Isolationand characterization of uterine BECs and LECs expressing tsA58T...