... to the great number of linkages between the enzyme andthe support and to the interference of hydrophilic residue ofthe carrier with the hydrophobic groups ofthe enzymatic protein The mode of ... for cofactors, substrate delivery, and product removal, may all contribute to the environment and thus to the performance ofthe enzyme product removal The surface ofthe carrier, whether inorganic ... into the enzyme active site may be the result of restricted movement ofthe substrate to the otherwise available active site ofthe enzyme or ofthe orientation ofthe enzyme in relation to the...
... production [7, 27] These reports, however, lack information on the biochemical and kinetic properties ofthe secreted enzymes Such information may be useful in gaining better understanding ofthe lignocellulose ... 600C and 150 rpm for 120 h andthe enzyme activities determined thereafter 2.6 Enzyme assays The cellulase and xylanase activity were determined by the assay method of Dutta et al [31] and Cheng ... (filer paper), BWX and PWSD The enzyme kinetic studies for cellulase and xylanase (Km and Vmax) were performed with 1-10 mg/ml of CMC and BWX, respectively [35] The crude cellulase and xylanase were...
... within the RATEs are catalytically active and that the activity decrease [16] is a result ofthe tandemization Accessibility ofthe linker of SGRSGRSG-RATE in the presence of RI The accessibility of ... molecular surfaces, performed with the program sc from the ccp4 suite [27] The obtained sc value of 0.400 indicates weak binding ofthe two entities ofthe RATE Therefore, the molecule might become ... relaxed in solution, thereby enabling RI binding to both the N-terminal andthe C-terminal RNase A entities The distance between the C-terminus ofthe first andthe N-terminus ofthe ˚ second RNase...
... generalisations, the first and third properties leading to the theory of saturated formations and Schunck classes andthe associated projectors andthe second property to the theory of Fitting classes and ... because ofthe preceeding argument and applying [Hup67, III, 2.11].This completes the induction step and with it the proof ofthe lemma Proof (of Theorem 2.2.6) Assume that the result is not true and ... presented The theory of formations was born The new “covering subgroups” had many ofthe properties ofthe Sylow and Hall subgroups, but the theory was not arithmetic one, based on the orders of subgroups...
... an oven and heated at 130°C for h After reaction, the foil was taken out ofthe autoclave and washed with water for several times and then dried in air X-ray diffraction (XRD) pattern ofthe products ... in the experiment It gives another evidence forthe formation of high purity ZnO product During hydrothermal synthesis of 1-D nanostructures, it was always found that the salt ions existed in the ... solution andthe pH values ofthe solution had great influences on the final products [42, 43] Figure 3a, b is the SEM images ofthe product obtained when zinc chloride was used instead of zinc...
... (1) where λ is the wavelength; h is the distance between the middle planes ofthe graphene andthe substrate surface; Ag and As are the amplitudes ofthe graphene morphology andthe substrate ... lithographically patterned for fabrication of devices The atomic force microscopy images indicated the formation of 2-3 layers of h-BNC The structures and compositions ofthe films were characterized ... C-N Therefore, the issue of stability is the most prominent here The number of strong bonds is sufficiently large in structure V forthe stabilization despite of 60◦ arrangement of B-N bonds Another...
... publish reliable data and information, but the author andthe publisher cannot assume responsibility forthe validity of all materials or forthe consequences of their use Neither this book nor ... kinetics, and mechanism ofthe enzyme can be brought together in the rational design of an inhibitor However, the transfer of a prospective drug candidate from the laboratory bench to the marketplace ... chemistry at the University of London and received his D.Sc in 1995 A fellow ofthe Royal Society of Chemistry andthe Royal Pharmaceutical Society of Great Britain, he has spent much of his career...
... axis of one ofthe profiles using the other profile as the reference One ofthe replicate spectra from the control condition is chosen as the reference to which the other spectra are warped The ... by incubation for 30 sec This step was followed by the addition of 400 µL of Chloroform and 300 µL of 1M HCL and vortexed for 30 sec The extraction of lipids at acid phase helps in the enhanced ... to the pellet followed by 200 µL of Phenol : Chloroform (1:1) mix The mixture was vortexed in the presence of Glass beads for – Then, 200 µL of TE buffer was added and spun for at 13,000 rpm The...
... mechanism of Af M1PDH and Af M2DH The theory developed by Cook and Cleland is used to deduce the kinetic mechanism of AfM1PDH and AfM2DH from KIE data in Table [28] The pattern of KIEs observed for ... represents a mean value and standard deviation for three independent experiments evaluated in either ofthe ways described above The KIE on kcat Km for Fru was calculated from the kcat and Km values obtained ... of Fru concentrations (40800 mm), and so the choice ofthe constant level of Fru was not critical for determination of DKm (1.23 0.15) The KIE data in Table are instrumental in delineating the...
... crushing quartz and used for sandpaper The chief types of abrasive sand include sandblast sand, glass-grinding sand, and stone-cutting sand Sand for stone sawing andfor marble and glass grinding ... surface; the shadow, light, and 3D visual effect of granite; the look of gun metal; andthe glitter of quartz or gold dust A gum resin from the base ofthe tufted trunk leaves of various species of ... have the radical group ᎏCHO in the molecule, and because of their ease of oxidation they are important reducing agents They are also used in the manufacture of synthetic resins and many other...
... represents a standard mixture of GlcNAc oligomers showing the standard 60 : 40 ratio between the a-anomer andthe b-anomer at equilibrium The other panels show the results of partial hydrolysis of (GlcNAc)6 ... the barley andthe jack bean enzymes, ChiG contains an N-terminal extension of eight and seven residues respectively (Fig 1) The N-termini ofthe plant enzymesandthe first residue in the ChiG ... located on the opposite side ofthe enzyme to the catalytic center andthe substrate-binding groove The same applies to the structurally observed N-terminus ofthe catalytic domain of ChiC, which...
... parasites ofthe genus Giardia are one ofthe earliest lineages of eukaryotic cells, andthe Giardia protease is the earliest known branch ofthe cathepsin B family [23] Its phylogeny confirms that the ... concentrating the allergen according to the isoelectric point (pI) ofthe molecule A pH gradient from to 11 was established forthe characterization of Phl p After completion ofthe run, the individual ... cysteine residues and other essential amino acids are numbered Areas of high similarity exist around Cys69 and Cys72 and around Trp186, Trp193 and Trp197 The positions of Cys41, 57, 69 and 72 are especially...
... Suggestions for or descriptions ofthe end use or application of products or methods of working are for information only andthe company and its subsidiaries accept no liability in respect thereof Before ... knowledge ofthe properties of different phases, it is possible to judge the extent to which they affect the service life ofthe weldment The diagram can be used for rough estimates ofthe weldability ... The melting ofthe wire also reduces the temperature ofthe molten metal, which also reduces the risk of hot cracking For MIG welding of common steels of type AISI 304, 316, 304L and 316L, wire...
... crushing quartz and used for sandpaper The chief types of abrasive sand include sandblast sand, glass-grinding sand, and stone-cutting sand Sand for stone sawing andfor marble and glass grinding ... surface; the shadow, light, and 3D visual effect of granite; the look of gun metal; andthe glitter of quartz or gold dust A gum resin from the base ofthe tufted trunk leaves of various species of ... have the radical group ᎏCHO in the molecule, and because of their ease of oxidation they are important reducing agents They are also used in the manufacture of synthetic resins and many other...
... with the value ofthe bulk SnO2 Quantification ofthe Sn3d and O1s peaks gives an average Sn/O atomic ratio of 1:1.95, indicating the O-deficient formation ofthe SnO2 nanowires Fig XRD pattern of ... 1c) clearly indicates that these nanowires are of uniform size and smooth surface, andthe average diameter and length of these nanowires are 80 nm and m, respectively The energy dispersive X-ray ... shown in the inset (c) TEM-based EDS spectrum ofthe single nanowire (d) HRTEM image ofthe nanowire TEM image (Fig 4a) clearly indicates that nanowires are of smooth surface and rather uniform size...
... result in the processing line being stopped Another undesirable example is when the force of adhesion ofthe product to packaging material or to another slice ofthe product is higher than the breaking ... performed When samples did not fit the normal distribution, the Kruskal–Wallis test was used to test the null hypothesis that the medians of variables within each ofthe levels of samples were the ... in the range of 1–3 N/cm2 and 4– 12 N mm, respectively About 50% of cooked meat sausages showed BS and EF values within these intervals The galantines had low-values of BS and EF, except for the...
... than the MCID forthe EMS andthe original version ofthe HABAM but not forthe PPME These data were not available forthe Rasch refined version ofthe HABAM Validity The PPME has face and content ... in the final stages of drafting ofthe manuscript and given approval forthe version to be published JK contributed to the conception and design ofthe review, the analysis and interpretation of ... forthe HABAM Although the feasibility ofthe EMS has not been investigated, the HABAM has fewer equipment requirements than the EMS and PPME and is therefore likely to be the more feasible of...
... and Sa The effects of pull and heat are very similar and not as significant as the effect of velocity Delay and pressure are factors to change the taper length ofthe pipettes while keeping the ... control measures the velocity ofthe glass carriage system as the glass softens By increasing the velocity, both the tip size andthe surface roughness decrease The velocity has the most significant ... http://www.nanoscalereslett.com/content/6/1/401 Page of 10 Dt Heat Figure The effect of heat on tip diameter and average surface roughness The heat is controlled by the level of electrical current supplied to the filament The unit of heat...
... function ofthe thermal conductivities ofthe base fluid andthe nanoparticles, the volume fraction, fractal dimension for particles, the size of nanoparticles, andthe temperature, as well as random ... ofthe nano-copper suspensions, while the effect ofthe mass fraction of Cu on the viscosity is not as obvious as that ofthe temperature and SDBS dispersant forthe mass fraction chosen in the ... forthe large thermal conductivity enhancement of nanofluids [47] However, the Brownian motion contribution to the thermal conductivity of nanofluid was very small and cannot be responsible for...
... surface of FeOOH nanorods makes the surface smoother and leads to the reduction ofthe BET surface area In order to confirm the effect of layer-by-layer process on the formation ofthe uniform FeOOH–Au ... in the final products, and finally dried at 80°C in air Forthe synthesis of a-Fe2O3–Au nanorods, the as-prepared FeOOH–Au nanorods were heated to 500°C for h in air While forthe synthesis of ... From the result of BET analysis, we can find that the BET surface area decrease after the layer-by-lay assembly The possible reason for this phenomenon is that the polyelectrolyte deposited on the...