iron metabolism in pathogenic trypanosomes
Báo cáo khoa học: Enzymes of mannitol metabolism in the human pathogenic fungusAspergillus fumigatus– kinetic properties of mannitol-1-phosphate 5-dehydrogenase and mannitol 2-dehydrogenase, and their physiological implications pot
... domains. The N-terminal domain adopts anexpanded Rossmann fold, and provides the residues forbinding the coenzyme. The C-terminal domain has a lar-gely a-helical structure, and serves mainly in ... ofinitial-rate data recorded at different concentrations ofinhibitor. The observed inhibition was, in each case,best described by competitive binding of coenzyme andadenine nucleotide. Inhibition ... conditions. Lineweaver–Burk plots of initial-rate data(Fig. 1) gave a pattern of intersecting lines, for eachenzyme and in both reaction directions, consistent witha kinetic mechanism in which...
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Tài liệu Báo cáo khoa học: Emerging pathways in genetic Parkinson’s disease: Potential role of ceramide metabolism in Lewy body disease pptx
... calcium-independentphospholipase A2participates in ER stress-inducedINS-1 insulinoma cell apoptosis by promoting cera-mide generation via hydrolysis of sphingomyelins byneutral sphingomyelinase. Biochemistry 46, ... glucosylceramide synthase; SMS, sphingomyelin synthase; SMse, sphingomyelinase; SPT, serine palmitoyl transferase. Yellow repre-sents enzymes directly involved in ceramide metabolism, in which mutations are ... mutation in the gene encodingalpha-synuclein in Parkinson’s disease. Nat Genet 18,106–108.42 Singleton AB, Farrer M, Johnson J, Singleton A,Hague S, Kachergus J, Hulihan M, Peuralinna T,...
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Tài liệu Báo cáo khoa học: Implication of the glutamine synthetase ⁄glutamate synthase pathway in conditioning the amino acid metabolism in bundle sheath and mesophyll cells of maize leaves doc
... the main nitrogen compounds in thephloem exudates, amounting to 51% of the totalamino acids in the light and 56% in the dark(Fig. 7A). The total amount of alanine, serine and gly-cine was ... Staining was found in the cyto-plasmic layers of BSCs (Fig. 4A). No positive stainingwas detected in the BSCs using the control sense probe(Fig. 4B). The localization of GLU mRNAs in thevicinity ... ofglutamine and asparagine in the phloem seemed to beincreased in the dark (Table 1). Second, glutamate,aspartate and serine were similarly distributed in leavesand phloem sap, yielding phloem...
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Tài liệu Báo cáo khoa học: On the mechanism of action of the antifungal agent propionate Propionyl-CoA inhibits glucose metabolism in Aspergillus nidulans doc
... cells), even in themutant strain.Determination of the intracellular volume In order to obtain the intracellular concentration ofaccumulated acyl-CoA esters, it was necessary to knowthe internal ... lmolNADPặmin)1ặmg protein)1.Determination of maintenance In order to calculate the amount of glucose used formaintenance, the wild-type strain A26 was used. Four100-mL aliquots of glucose minimal ... acetate to the media containing glucoseand propionate, the growth rate of both strains increasedand the effect of propionate became less apparent. Finally, in media containing acetate and propionate...
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Tài liệu Báo cáo khoa học: Elementary modes analysis of photosynthate metabolism in the chloroplast stroma ppt
... thioredoxinsystem [10,11]. Thioredoxin is a small, redox active protein,capable in turn of reducing or oxidizing disulphide bonds in proteins. In chloroplasts, thioredoxin is reduced byferredoxin, ... authors investigated dark stromal metabolism in chloroplasts isolated from Mesembryanthemum crystallinum.This plant is interesting in that it is capable of operating C3orCAM (crassulacean acid metabolism) ... wheatsedoheptulose-1,7-bisphosphatase in which the regulatorycysteines were mutated to serines, rendering the resultingproduct insensitive to thioredoxin (unpublished data).Such a change will impact in two ways on...
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IRON METABOLISM Edited by Sarika Arora pptx
... have provided insight into the mechanisms by which hepcidin modulates iron absorption. Within a week of being placed on a low -iron diet, rats show a twofold increase in intestinal iron absorption ... or administration of recombinant hepcidin [51]. The second involves binding of hepcidin to FPN at the cell membrane, causing internalization and degradation of FPN, thus diminishing iron transfer ... hepcidin on intestinal iron absorption. Since the discovery of hepcidin, several authors have reported that hepcidin expression fails to increase in response to increased iron stores in other...
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Báo cáo khoa học: Enzymes of creatine biosynthesis, arginine and methionine metabolism in normal and malignant cells Soumen Bera1, Theo Wallimann2, Subhankar Ray1 and Manju Ray1 potx
... creatine kinase in the same malignant cells do not appear tobe sufficient for the building up of an effective creatine ⁄ phosphocreatinepool. Instead of supporting creatine biosynthesis, l-arginine:glycine ... 91–95.45 Van Pilsum JF, Martin RP, Kito E & Hess J (1956)Determination of creatine, creatinine, arginine, guanidi-noacetic acid, guanidine, and methyl-guanidine in bio-logical fluids. J Biol ... compounds are in high demand in proliferating cancer cells.Abbreviations3MC, 3-methylcholanthrene; AGAT,L-arginine:glycine amidinotransferase; CK, creatine kinase; CT-1, creatine transporter;...
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Thyroid hormone metabolism in poultry ppt
... abundant in liver, kidney and intestine, medium in several othertissues and absent in muscle, skin and brain. The D1activity increases in liver and intestine towardshatching but decreases in gonads ... is(mono)deiodination where one iodine is removed fromthe outer ring (outer ring deiodination or ORD) or fromthe inner ring (inner ring deiodination or IRD) of aiodothyronine molecule. Deiodination ... levels to reach aminimum within 30 min after injection, proving theinvolvement of pretranslational regulation (Van derGeyten et al., 1999a). Additional studies includingactinomycine D and cycloheximide...
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Báo cáo Y học: Molecular interaction of neutral trehalase with other enzymes of trehalose metabolism in the fission yeast Schizosaccharomyces pombe pdf
... SpainTrehalose metabolism is an essential component of the stressresponse in yeast cells. In this work we show that the prod-ucts of the principal genes involved in trehalose metabolism in ... be integrated mechanisms.These findings indicate a divergence in the molecular designscontrolling trehalose metabolism in S. pombe from those in S. cerevisiae.MATERIALS AND METHODSStrains ... TPS1–TPS2complex in S. cerevisiae, it is tempting to speculate that in S. pombe these ORFs code for proteins that somehowregulate trehalose metabolism by interacting with somemembers of the enzyme proteins...
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Báo cáo khoa học: Enzymatic features of the glucose metabolism in tumor cells ppt
... phosphatidylinositol-3-kinase Aktand mitogen-activated protein kinase are candidate sig-naling pathways that mediate FASN expressionthrough the sterol regulatory element binding protein1c. In breast ... be induced by incubating cancer cellswith oligomycin, an inhibitor of mitochondrial ATPsynthetase [159,160]. Similarly, reduction of OXPHOSby targeted disruption of frataxin, a protein involved in ... enzyme becomes inactiveupon phosphorylation either by the cAMP-dependentprotein kinase A or by the insulin-dependent glycogenA. Herling et al. Tumor specific alterations in metabolism FEBS...
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Báo cáo khoa học: Transcriptional regulation of the desferrioxamine gene cluster of Streptomyces coelicolor is mediated by binding of DmdR1 to an iron box in the promoter of the desA gene doc
... the iron box), explaining the regulation of desAexpression by binding of DmdR1 to the iron box. Thesame overlapping was found in S. pilosus.DmdR1 binds to the promoter region of desABCD in ... evidence for or against this claim. SCO2780encoding a putative secreted siderophore-bindinglipoprotein with a conserved Fhu (Fe2+siderophorebinding) domain showed an iron- limitation responsesimilar ... desferrioxamine cluster.Indeed, binding of the pure DmdR1 protein to theS. coelicolor desA promoter region was shown for thefirst time in this work. As described previously, bind-ing of DmdR1 to the iron...
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Báo cáo khoa học: Pyruvate metabolism in rat liver mitochondria What is optimized at steady state? pptx
... the input ⁄ output relationship.Note that by constructing the conversion cone oneloses no information because all other, less interesting,details are contained in the current matrix.Assuming ... allowed input ⁄ output behaviour, andindeed any possible input ⁄ output behaviour isobtained as a linear combination (with non-negativescalar coefficients) of these 37 rows of P. Such linearcombinations ... which arean integral part of a cell in an organ such as the liver.As already mentioned, such biochemical entities aretightly integrated in a constantly changing cellularenvironment. In other...
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IRON METABOLISM pdf
... elements are present in living cells. Most of the iron in the body is located within the porphyrin ring of heme, which is incorporated into proteins such as hemoglobin, myoglobin, cytochromes, ... review on Cellular Iron metabolism with a Iron Metabolism 6 Fig. 3. Structure of ferritin showing the outer polypeptide shell with inner iron- core containing iron stored as mineral –ferric ... showing the physiological turnover of iron in the body 5. Mechanisms regulating Iron absorption The iron stores in the body are regulated by intestinal absorption. Intestinal absorption of iron...
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Báo cáo khoa học: A comparative study of methylglyoxal metabolism in trypanosomatids potx
... following oral dosing. Using thismethodology, we examined the effects of methylglyoxalon an in vivo T. brucei infection. The maximum achiev-able methylglyoxal concentration in blood followingoral ... 72 h. Concentrations of inhib-itor causing a 50% reduction in growth (EC50) were deter-mined using the following two-parameter equation bynonlinear regression using grafit:y ẳ1001 ỵẵI=EC50ịmwhere ... Solidlines: confirmed metabolism in T. brucei.Dotted lines: metabolism absent in T. bru-cei. MeGDH, methylglyoxal dehydrogenase;LDH, lactate dehydrogenase.Methylglyoxal metabolism in trypanosomatids...
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Báo cáo khoa học: P NMR studies of energy metabolism in xanthosine5¢-monophosphate overproducing Corynebacterium ammoniagenes pot
... optimized to gain maximum XMPproduction (Table 1). As shown in Table 1, an increase in the glu/glc ratio (glu/glc) induced a significant increase in XMP production, and this increase in XMP production ... accumulatedduring the glu-consuming phase (phase I), but muchaccumulated during the glu-deficient phase (phase II),suggesting that hypoxanthine production was inducedthrough a switch in the carbon flux. In ... specifically occurs during XMP production. In fact, an increase in ATP concentration and ATP/ADP ratiocould be induced during phase I by increasing glu concen-tration in the culture medium (Fig. 4). Dauner...
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