intracellular sites of damage
Báo cáo khoa học: End-damage-specific proteins facilitate recruitment or stability of X-ray cross-complementing protein 1 at the sites of DNA single-strand break repair docx
... next stage of repair Therefore, Pol b initiates repair of single-nucleotide gaps, PNK is required for the initiation of repair of 3¢-phosphatecontaining SSBs, and APE1 initiates repair of 3¢-phosphoglycolate-containing ... stimulates binding ⁄ crosslinking of XRCC1–DNA ligase IIIa heterodimer during the repair of incised AP sites that are intermediates of BER [28] Conversely, immunodepletion of XRCC1– DNA ligase IIIa heterodimer ... by nucleation of a transient damage- specific complex including a subset of enzymes that are required for repair of a particular lesion Our data not provide strong evidence in favour of transient...
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Báo cáo lâm nghiệp: "he extent of root rot damage in Norway spruce stands established on fertile sites of former agricultural land" pot
... Classification of stumps according to the degree of root rot damage Class Extent of rot damage healthy, no signs of fungal attack on the stump cutting area small area of hard rot up to 10% of stump ... regardless of previous damage to stems caused by game The extent of root rot was even greater in two stands without deer barking damage, in the vicinity of the village of Dvorce The least extent of root ... was to compare the degree of root rot damage in two large complexes of Norway spruce stands established on former agricultural land at fertile sites in two upland regions of Moravia The basic idea...
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Tài liệu Báo cáo khoa học: Active and regulatory sites of cytosolic 5¢-nucleotidase doc
... sites, one for ATP, one for ADP and one for BPG [5,12] On the basis of the results obtained from crystallization of a truncated form of cN-II [11], we constructed point mutants for a number of ... disappearance of the heavier inactive peak Furthermore, FPLC analysis of the recombinant wild-type cN-II showed the presence of the tetrameric active form irrespective of the presence of effectors ... an alteration of this ratio for a mutant was considered as caused either by an alteration of the Km value for one of the two substrates or by a variation of the kcat value for one of the two activities...
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Tài liệu BIOCHEMICAL TARGETS OF PLANT BIOACTIVE COMPOUNDS A pharmacological reference guide to sites of action and biological effects doc
... the initial use for cardiac insufficiency of digitalis (dried leaves of the foxglove, DZpitalispurpureumn) Determining the molecular sites of action of bioactive medicinal plant constituents ... kill plant-consuming organisms 1.3 Description of the tables Most of the book is comprised of tables dedicated to specific targets or groups of targets of plant defensive compounds Target-related ... per litre) Of course, the quarltitation of such affinities depends upon the conditions of measurement and the source of the biochemical target entity However, it was felt that provision of such...
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Tài liệu Báo cáo khoa học: Competition between innate multidrug resistance and intracellular binding of rhodamine dyes pdf
... various intracellular sites Likely sites are the intracellular membranes because they are hydrophobic and negatively charged A comparison of rhodamine uptake, in the presence and the absence of uncouplers, ... to determination of the amount of dyes associated with them The advantages of such an assay are that: (a) it is quantitative; (b) it is not affected by intracellular quenching of the dyes; and ... the presence of ATP [25]; thus, the increased uptake of rhodamine dyes observed in the presence of NBD-Cl does not appear to be due to the inhibition of Pgp A half maximal effect of NBD-Cl was...
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Tài liệu Báo cáo khoa học: The intracellular region of the Notch ligand Jagged-1 gains partial structure upon binding to synthetic membranes docx
... SDS, and of random coil values for a protein of the same sequence Fig S2 In-cell NMR 1H-15N HSQC spectra of the E coli slurry [(A), not induced; (B), induced and of the supernatant (C) of the induced ... Effect of pH Far-UV CD spectra of J1_tmic (7.5 lm) in the presence of SDS (3 mm) at 5336 pH 7.4, after acidification to pH 6, and after return to pH 7.4 Fig S6 Effect of pH Far-UV CD spectra of J1_tmic ... 1H-15N HSQC spectrum of the 15N-labeled protein, only 90 backbone HN cross-peaks of the expected 125 are detectable ( 70%), most of them clustered in a narrow region of between 7.7 and 8.4...
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Tài liệu Báo cáo Y học: Regulated expression and intracellular localization of cystatin F in human U937 cells pptx
... out of three with the same trend of results), intracellular levels (black bars) of cystatin F were approximately 3-fold lower than the extracellular levels (grey bars) both after and days of incubation ... is an underestimate of the real production of cystatin F in U937 cells The high portion of intracellular cystatin F led us to fractionate cells to determine the localization of the inhibitor Initially ... in agreement with analysis of cystatin F EST clones in a collection of > 650 cDNA libraries, showing that 54 cystatin F clones were present in 20 of the libraries, all of which were derived from...
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Tài liệu Báo cáo Y học: Identification of syntaxin-1A sites of phosphorylation by casein kinase I and casein kinase II ppt
... The mass spectrometry of radioactive peptides showed the presence of a doubly charged phosphopeptide of mass 974.0 and a singly charged phosphopeptide of mass 1946.3 both of which correspond only ... is the kinase Fig Summary of phosphorylation sites on recombinant syntaxin-1A The figure shows the sites of phosphorylation by CKIa (Thr21) and CKII (Ser14) The masses of the major peptides, in ... (absorbance at 210 nm) with positions of 32 P-labelled tryptic peptides of syntaxin-1A (1–190) phosphorylated by CKIa A C-terminal truncated construct of mutant of syntaxin-1A containing residues...
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Báo cáo khoa học: N-Glycosylation is important for the correct intracellular localization of HFE and its ability to decrease cell surface transferrin binding pptx
... absence of tunicamycin Drug treatments resulted in the accumulation of forms of the mutant proteins that were of lower apparent molecular mass and of similar size to the unglycosylated form of the ... glycosylation at specific sites A good example of this is TfR1, in which mutation of one of the three glycosylation sites (Asn727) results in a reticular rather than ERC pattern of localization [41,42] ... end of an a-helical region of the HFE a1 domain (V100 and W103A) as being of particular importance for TfR1 interactions [16,17,20] The effect of HFE binding to TfR1 is to lower the affinity of...
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Báo cáo khoa học: Nanoparticles can induce changes in the intracellular metabolism of lipids without compromising cellular viability docx
... synthesis of palmitate, the precursor of FFAs The inhibition of signaling by trophic factors, removal of trophic factors or inhibition of fat synthesis result in the down-regulation of lipogenesis ... 24 h with lgÆmL)1 of cerulenin (B), lgÆmL)1 of CdTe QDs (C) and both lgÆmL)1 of CdTe QDs and lgÆmL)1 of cerulenin (D) The scale bars correspond to 50 lm (E) The quantification of the lipid droplet ... studied the effects of QDs on living cells and have reported their internalization, the intracellular production of reactive oxygen species (ROS) and the damage to multiple cellular sites induced by...
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Báo cáo khoa học: The enantioselectivities of the active and allosteric sites of mammalian ribonucleotide reductase pptx
... Lineweaver–Burke plot of L-ADP inhibition of dGTP-dependent D-ADP reductase in the presence of mM D-ATP (B) Secondary plot of the slope values of A (C) LineweaverBurk plot of L-ADP inhibition of dGTP-dependent ... investigations of interactions of l-nucleosides or l-nucleotides with allosteric sites have been reported (although see [13]) In the present work, we examine the abilities of l-ATP and of l-ADP (Fig ... mostly in the form of active mR16 [2] Earlier we showed that, under similar conditions, addition of dATP led to inhibition of activity, largely as a result of dATP displacement of d-ATP from the...
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Báo cáo khoa học: Homoadenosylcobalamins as probes for exploring the active sites of coenzyme B12-dependent diol dehydratase and ethanolamine ammonia-lyase docx
... mechanism of action of adenosylcobalamin-dependent glycerol dehydratase from Aerobacter aerogenes I Role of structural components of adenosylcobalamin in the formation of the active site of glycerol ... of the Co-C bond [17] (Fig 1A) Difference in the specificity for the adenosyl group among enzymes may reflect the difference of the adenosyl group-binding sites of the enzymes From simulation of ... with those of free counterparts The extents of the Co-C bond cleavage of these analogues were negligible although that of AdoCbl was estimated to be 85% from Fig 3E Upon denaturation of the enzyme-cobalamin...
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Báo cáo khoa học: UDP-galactose 4-epimerase from Kluyveromyces fragilis – catalytic sites of the homodimeric enzyme are functional and regulated docx
... of NAD+ to NADH by reductive inhibition of epimerase offered a sensitive method for determination of stoichiometry of the bound cofactor Reductive inhibition was applied to 33.3 nmol (5 mg) of ... uncertainty of protein concentration determination and the incompleteness of conversion of NAD+ to NADH Therefore, with respect to the composition of cofactor, the catalytic sites of epimerase ... stoichiometry of two NAD+ molecules per dimer, similarly to E coli and human epimerase, raising the possibility of the existence of two catalytic sites Binding of one molecule of nondialyzable...
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Báo cáo khoa học: The use of synthetic linear tetrapyrroles to probe the verdin sites of human biliverdin-IXa reductase and human biliverdin-IXb reductase potx
... concentration of cytosolic biliverdin-IXa The paucity of information on the biliverdin binding site has hampered the development of inhibitors of BVR-A With this in mind, and in the absence of a crystal ... solution of ester (blue); addition of 37 lM BSA from a stock solution of 50 mgÆmL)1 produced the red trace and subsequent addition of 50 lM NADPH produced the green trace After addition of enzyme, ... the red shift and doubling of extinction coefficient on the addition of HCl [18] The dimethyl ester of biliverdin-IXa was synthesized by oxidation of the free acid of bilirubinIXa to biliverdin-IXa...
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Báo cáo khoa học: Functional significance of five noncanonical Ca2+-binding sites of human transglutaminase 2 characterized by site-directed mutagenesis potx
... Ca2+-binding sites of TG2 ´ly R Kira et al Fig Multiple sequence alignment of Ca2+-binding sites of transglutaminases Sequence alignments of Ca2+-binding sites of TG2 compared with the other members of ... site of wild typeTG2 0.0 Weak binding sites of wild type TG2 Weak binding sites of S1 TG2 0.0 0.00 –0.1 –0.3 n = 0.52 ± 0.63 Kd = 0.1 ± 0.03 µM –0.4 kcal·mol–1 of injectant –0.2 kcal·mol–1 of injectant ... confirmed that each of the five mutagenized sites contributes to Ca2+ binding of TG2 It was also observed that disruption of one site by mutation leads to weaker ⁄ loss of binding to other sites, and this...
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Báo cáo khoa học: Intracellular degradation of somatostatin-14 following somatostatin-receptor 3-mediated endocytosis in rat insulinoma cells doc
... FEBS 4729 Intracellular degradation of somatostatin D Roosterman et al A B C D E F Fig Loss and recovery of SST-14 binding sites (A) SST-14-mediated reduction of cell surface binding sites RIN-SSTR3 ... downregulation of the receptor After a period of 1300 of stimulation and 120 of recovery, cell surface binding was reduced to 43 ± 5% The time-dependent loss of surface binding sites during stimulation ... 4730 number of binding sites After stimulation with SST-14, cell surface binding decreased to 52 ± 3% of that of untreated cells, whereas total binding remained at 91 ± 4% of that of untreated...
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Báo cáo khoa học: Classification of ATP-dependent proteases Lon and comparison of the active sites of their proteolytic domains pdf
... T.V (2001) Coupling of proteolysis and hydrolysis of ATP upon functioning of Lon proteinase of Escherichia coli II Hydrolysis of ATP and activity of peptide hydrolase sites of the enzyme Bioorgan ... prompted a reanalysis of the active sites of this family of proteases The alignment of the proteolytic domains derived from the sequences of > 100 Lon proteases from a variety of sources provided ... Biochem 271) Ser679, the catalytic site of E coli Lon Experimental verification of the role of different residues led to the preparation of a series of mutants of amino acids in E coli Lon that were...
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Báo cáo khoa học: Cytosolic phospholipase A2-a and cyclooxygenase-2 localize to intracellular membranes of EA.hy.926 endothelial cells that are distinct from the endoplasmic reticulum and the Golgi apparatus pdf
... levels of cPLA2-a expression and site of cPLA2-a relocation Comparison of the location of cPLA2-a in EA.hy.926 endothelial cells with markers for specific cellular organelles The relocation of cPLA2-a ... has not been studied, and this is one of few studies that shows distinct colocalization of cPLA2-a with a COX isoform Functional coupling of cPLA2-a and COX isoforms in endothelial cells has not ... inclusions, which are structurally distinct sites of esterified arachidonic acid and thus represent a nonmembrane site of eicosanoid generation COX isoforms have also been found in similar cytosolic...
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Báo cáo khoa học: Identification of sites of phosphorylation by G-protein-coupled receptor kinase 2 in b-tubulin ppt
... phosphorylation sites for GRK2 are Thr409 and Ser420, but not Ser413 Phosphorylation by GRK2 of GST fusion proteins of full-length bI-tubulin, bIII-tubulin, and C-terminal peptides of bI-tubulin ... for GST-bI-tubulin and Ó FEBS 2003 Sites of phosphorylation by GRK2 in tubulin (Eur J Biochem 270) 1159 Fig Phosphorylation by GRK2 of GST fusion proteins of bI-tubulin and bIII-tubulin (GST-b-tubulin) ... phosphorylation of tubulin purified from porcine brains (0.4–3 lM) [19–21] Phosphorylation by GRK2 of GST fusion proteins of b-tubulin mutants To confirm that Thr409 and Ser420 are the only phosphorylation sites...
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Báo cáo Y học: The mitochondrial-lysosomal axis theory of aging Accumulation of damaged mitochondria as a result of imperfect autophagocytosis ppt
... majority of senescent changes in postmitotic cells INTRALYSOSOMAL ACCUMULATION OF LIPOFUSCIN Deposition of lipofuscin pigment within postmitotic cells, one of the most prominent signs of aging, ... 150 years [28] However, the relation of lipofuscin to lysosomes and the basic mechanisms of lipofuscin formation have been determined only recently [29,30] Lipofuscin accumulates within lysosomes, ... [31,32] Lipofuscin is a mixture of substances, mainly of protein and lipid origin (30–70% and 20–50%, respectively) It also contains small amounts of carbohydrates (up to 7%) and traces of metals,...
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- content of damage stability booklet
- harmful effects of damaged ozone layer
- mtdna analysis the new frontier in dna profiling and the sequencing of polymorphic sites of the nuclear and mitochondrial genome
- sites of deterioration mechanisms
- three sites of the construction of civil liberties in the new constitutional nation
- memory sites of sorrow an american veterans motorcycle pilgrimage
- k concentration is relatively small compared with the intracellular concentration of k
- search for sites of action
- beyond the sequon sites of n glycosylation
- investigation negotiation and the quantum of damage
- an exemplary probe of nucleotide binding to the catalytic sites of f1
- liposome dna complex and intracellular visualization of the complexes
- intracellular injection of mrna
- 5 apos start sites of pa and pc
- intracellular fate of different protozoal species
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