intracellular pharmacokinetics of gemcitabine
Tài liệu Báo cáo khoa học: Competition between innate multidrug resistance and intracellular binding of rhodamine dyes pdf
... to determination of the amount of dyes associated with them The advantages of such an assay are that: (a) it is quantitative; (b) it is not affected by intracellular quenching of the dyes; and ... the presence of ATP [25]; thus, the increased uptake of rhodamine dyes observed in the presence of NBD-Cl does not appear to be due to the inhibition of Pgp A half maximal effect of NBD-Cl was ... the affinity of rhodamines to intracellular membranes is not very different from their affinity towards the extracellular surface of the plasma membrane Because the surface area of intracellular...
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Tài liệu Báo cáo khoa học: The intracellular region of the Notch ligand Jagged-1 gains partial structure upon binding to synthetic membranes docx
... SDS, and of random coil values for a protein of the same sequence Fig S2 In-cell NMR 1H-15N HSQC spectra of the E coli slurry [(A), not induced; (B), induced and of the supernatant (C) of the induced ... Effect of pH Far-UV CD spectra of J1_tmic (7.5 lm) in the presence of SDS (3 mm) at 5336 pH 7.4, after acidification to pH 6, and after return to pH 7.4 Fig S6 Effect of pH Far-UV CD spectra of J1_tmic ... 1H-15N HSQC spectrum of the 15N-labeled protein, only 90 backbone HN cross-peaks of the expected 125 are detectable ( 70%), most of them clustered in a narrow region of between 7.7 and 8.4...
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Tài liệu Báo cáo Y học: Regulated expression and intracellular localization of cystatin F in human U937 cells pptx
... out of three with the same trend of results), intracellular levels (black bars) of cystatin F were approximately 3-fold lower than the extracellular levels (grey bars) both after and days of incubation ... is an underestimate of the real production of cystatin F in U937 cells The high portion of intracellular cystatin F led us to fractionate cells to determine the localization of the inhibitor Initially ... in agreement with analysis of cystatin F EST clones in a collection of > 650 cDNA libraries, showing that 54 cystatin F clones were present in 20 of the libraries, all of which were derived from...
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Báo cáo khoa học: N-Glycosylation is important for the correct intracellular localization of HFE and its ability to decrease cell surface transferrin binding pptx
... absence of tunicamycin Drug treatments resulted in the accumulation of forms of the mutant proteins that were of lower apparent molecular mass and of similar size to the unglycosylated form of the ... end of an a-helical region of the HFE a1 domain (V100 and W103A) as being of particular importance for TfR1 interactions [16,17,20] The effect of HFE binding to TfR1 is to lower the affinity of ... sites of HFE N-glycosylation and examine the importance of glycosylation on parameters of protein localization and function Results Tunicamycin treatment results in a reticular pattern of HFE...
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Báo cáo khoa học: Nanoparticles can induce changes in the intracellular metabolism of lipids without compromising cellular viability docx
... synthesis of palmitate, the precursor of FFAs The inhibition of signaling by trophic factors, removal of trophic factors or inhibition of fat synthesis result in the down-regulation of lipogenesis ... 24 h with lgÆmL)1 of cerulenin (B), lgÆmL)1 of CdTe QDs (C) and both lgÆmL)1 of CdTe QDs and lgÆmL)1 of cerulenin (D) The scale bars correspond to 50 lm (E) The quantification of the lipid droplet ... degree of internalization of QDs and various other NPs is dependent on their size, surface charge, concentration in the medium and duration of exposure [4,7–11] We have also shown the release of...
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Báo cáo khoa học: Intracellular degradation of somatostatin-14 following somatostatin-receptor 3-mediated endocytosis in rat insulinoma cells doc
... the absence of ammonium chloride corresponding to 80% of the amount of the cell surface-bound peptide The amount of intracellular radioactivity then quickly declined over a period of 15 to ... process of the receptor, the fate of the peptide agonist has not thus far been analyzed after cointernalization with SSTR3 Intracellular degradation of somatostatin Therefore, we examined the fate of ... Localization of SSTR3 after chronic stimulation with SST-14 Chronic stimulation of SSTR3 with SST-14 mediates downregulation of SSTR3 The immunofluorescence signal of SSTR3 is concentrated in one area of...
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Báo cáo khoa học: Cytosolic phospholipase A2-a and cyclooxygenase-2 localize to intracellular membranes of EA.hy.926 endothelial cells that are distinct from the endoplasmic reticulum and the Golgi apparatus pdf
... levels of cPLA2-a expression and site of cPLA2-a relocation Comparison of the location of cPLA2-a in EA.hy.926 endothelial cells with markers for specific cellular organelles The relocation of cPLA2-a ... has not been studied, and this is one of few studies that shows distinct colocalization of cPLA2-a with a COX isoform Functional coupling of cPLA2-a and COX isoforms in endothelial cells has not ... shown) and to be dependent on an influx of extracellular calcium Much of the controversy over the exact site of cPLA2-a relocation has arisen due to a variety of antibodies and cell types being studied...
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Báo cáo khoa học: Alanine screening of the intracellular loops of the human bradykinin B2 receptor – effects on receptor maintenance, G protein activation and internalization pdf
... Although the processes of signaling and regulation of human B2R are, in general, fairly well understood, the knowledge of the molecular basis of these events at the structural level of the receptor ... compilation ª 2009 FEBS Ala screening of intracellular loops of the B2 receptor A Faussner et al Fig Mutated sequences in human B2R The sequences of the ICLs, parts of the transmembrane segments (I–VII) ... 3493 Ala screening of intracellular loops of the B2 receptor A Faussner et al Fig Construct expression levels (A) Maximal surface binding of [3H]BK to confluent monolayers of HEK 293 cells stably...
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Báo cáo sinh học: " Thiol-reactive reagents inhibits intracellular trafficking of human papillomavirus type 16 pseudovirions by binding to cysteine residues of major capsid protein L" pot
... concentration of mM 3B) The data indicate that BPEOIA bound to the free thiol of cysteine residue(s) of L1 Binding of BPEOIA to the L1 cysteine residues of 16PV BPEOIA, capable of making a complex ... containing mM of DTNB, MBTA or MTSET for days grew and were maintained 1 C C C CC C C C C Figure Alignment of L1 amino acid sequences of papillomaviruses Alignment of L1 amino acid sequences of papillomaviruses ... [2-(trimethylammonium)ethyl] methanethiosulfonate bromide 37K Figure Binding of BPEOIA to L1 of the 16PV Binding of BPEOIA to L1 of the 16PV (A) The 16PVs were incubated with mM biotin-PEO-iodoacetamide...
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Báo cáo sinh học: "Safety and pharmacokinetics of recombinant human hepatocyte growth factor (rh-HGF) in patients with fulminant hepatitis: a phase I/II clinical trial, following preclinical studies to ensure safety" ppt
... points The primary endpoint of interest was the safety of repeated doses of intravenous rh-HGF, which was evaluated on the basis of the occurrence, frequency, and severity of adverse events All patients ... single dose of rhHGF Page of 12 The secondary endpoints were the pharmacokinetics of intravenously injected rh-HGF and clinical efficacy, including survival period and outcome To examine pharmacokinetics ... Infusion of 100 mL of saline prior to rh-HGF administration prevented a decrease in BP during exposure to rh-HGF of capacitance vessels is a cause of HGF-induced BP reduction Evaluation of renal...
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Báo cáo sinh học: " Intracellular localization of Crimean-Congo Hemorrhagic Fever (CCHF) virus glycoproteins" docx
... Indirect immunofluorescence assays of CCHFV glycoproteins after transfection of different expression plasmids Indirect immunofluorescence assays of CCHFV glycoproteins after transfection of different ... Signals for intracellular targeting of CCHFV glycoproteins After determining the intracellular localization of the CCHFV glycoproteins, we next were interested to determine the signals for intracellular ... correct intracellular processing of the CCHFV GC we inserted the Ig κ-chain signal peptide of the pDisplay vector via forward oligonucleotide primer RF351 Intracellular localization of the glycoproteins...
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Báo cáo hóa học: " Thiol-reactive reagents inhibits intracellular trafficking of human papillomavirus type 16 pseudovirions by binding to cysteine residues of major capsid protein L1" pdf
... concentration of mM 3B) The data indicate that BPEOIA bound to the free thiol of cysteine residue(s) of L1 Binding of BPEOIA to the L1 cysteine residues of 16PV BPEOIA, capable of making a complex ... containing mM of DTNB, MBTA or MTSET for days grew and were maintained 1 C C C CC C C C C Figure Alignment of L1 amino acid sequences of papillomaviruses Alignment of L1 amino acid sequences of papillomaviruses ... [2-(trimethylammonium)ethyl] methanethiosulfonate bromide 37K Figure Binding of BPEOIA to L1 of the 16PV Binding of BPEOIA to L1 of the 16PV (A) The 16PVs were incubated with mM biotin-PEO-iodoacetamide...
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báo cáo hóa học:" Efficacy, Safety and Pharmacokinetics of Once-Daily Saquinavir Soft-Gelatin Capsule/Ritonavir in Antiretroviral-Naive, HIV-Infected Patients" pot
... pharmacokinetic profile of patients at week Intensive pharmacokinetic profile of patients at week EC50 = concentration for 50% maximal effect Page of 12 (page number not for citation purposes) Journal of the ... classification of efavirenz as pregnancy category D caution, it is advised in the treatment of women of child-bearing potential Also, efavirenz Page of 12 (page number not for citation purposes) Journal of ... therefore, consisted of 49 patients (5 patients with intensive pharmacokinetics and 44 with single pharmacokinetic evaluations) Page of 12 (page number not for citation purposes) Journal of the International...
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Báo cáo hóa học: " Functionalized halloysite nanotube-based carrier for intracellular delivery of antisense oligonucleotides" pdf
... PBS, dehydrated through a series Page of of alcohol concentrations (30%, 50%, 70%, 90%, 100%), embedded in Epon, and sliced to a thickness of 70 nm Images of the sliced images were recorded at ... the percentage of fluorescent cells out of the total number of cells Fluorescence was detected from the FAM labeled on ASODNs at 488-nm excitation In vitro cell toxicity assay of the f-HNT-ASODN ... HeLa cells To visualize intracellular uptake of the f-HNT-based-ASODN complex, fluorescence FAM- Page of labeled ASODNs were used for the complex formation A TEM image of HeLa cells after incubation...
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Báo cáo khoa học: "Modification of pharmacokinetics of cefotaxime in uranyl nitrate-induced renal damage in black bengal goats" pps
... T~P values indicated shorter persistence of the drug in tissue compartment of normal Modification of cefotaxime kinetics Table Pharmacokinetic parameters of cefotaxime following single intramuscular ... about twofold higher, while body clearance value (ClB) was 44.5 times lower in kidney damaged than that of normal goats resulting into longer persistence of the compound in blood Increases of BUN ... AK Pharmacokinetics of cefotaxime in experimentally induced kidney damage in goats Indian J Pharmacol 2003, 35, 173176 Jha K, Roy BK, Singh RCP The effect of induced fever on the biokinetics of...
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Báo cáo khoa học: "Alteration of pharmacokinetics of oxytetracycline following oral administration of Piper longum in hens" ppsx
... yb ti ni tnetnoc enirepip fo ecneserp eht rof dezidradnats saw mugnol P deyassa llit C 02− ta nezorf erew selpmas ehT noitarapes amsalp rof setunim 01 rof mpr 000,3 ta degufirtnec erew detcelloc ... msinagroorciM seitilibitpecsus tnereffid fo smsinagroorcim rof slavretni egasod suoirav ta sdrib yrtluop ni mugnol repiP fo noitartsinimda laro gniwollof deniatbo seulav citenikocamrahp fo sisab eht no ... enilcycartetyxo gnitca gnol a fo esu eht no seidutS D drofwarC ,JP erolB ,EG nothguoH ,NJ yelsaeB ,FM kivalS ,MH giwlleH ,PD segaW ,SW droffawS ,KJ seleekS 81 371-561 ,85 ,7991 locamrahponhtE...
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Báo cáo khoa học: "Influence of endotoxin induced fever on the pharmacokinetics of intramuscularly administered cefepime in rabbits doc
... )81140K6OP5 oN( hcraeseR cifitneicS rof eettimmoC hsiloP eht yb detnarg tcejorp fo trap a demrof etapida nasotihc eht gnizisehtnys rof ssecorp ehT slavretni h 42 ta semit ruof ro ecno rehtie yllaenotirepartni ... lahtnewoL 5431-9331 ,331 ,4891 lonummI J nikuelretni rof srotpecer fo noisserpxe eht rof deriuqer si nikuelretni :rotpecer negitna eht rof cificeps ydobitna lanolconom a yb decudni enil llec ... fo noitartsinimda ehT dnuof osla erew sllec T edon hpmyl ciretnesem fo egatnecrep eht ni segnahc emoS )1 elbaT( gk/gm 02 fo esod a ta etapida nasotihc fo snoitcejni ruof retfa deton saw tceffe...
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Báo cáo khoa học: "Clinical pharmacokinetics of norfloxacin-glycine acetate after intravenous and oral administration in pigs" doc
... hybrid rate constant of the slope of distribution phase; β is the hybrid rate constant of the slope of elimination phase; and ka is the hybrid rate constant of the slope of absorption Following ... serum concentration intercepts of biphasic i.v and p.o disposition curves; α, hybrid rate constants of the slope of distribution; β, hybrid rate constants of the slope of elimination; AUC, the area ... (F) after oral administration of NFLXGA was 31.10 ± 15.16% Pharmacokinetics of norfloxacin in pigs 355 Table Parmacokinetic parameters that describe the disposition of norfloxacin-glycine acetate...
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