interspike interval histogram characterization of synchronized cardiac sympathetic neural discharge and epileptogenic activity in the electrocorticogram of the cat
Tài liệu Báo cáo khoa học: Biochemical characterization of human 3-methylglutaconyl-CoA hydratase and its role in leucine metabolism docx
Ngày tải lên :
19/02/2014, 07:20
... enoyl-CoA-hydratase activity using 2014 crotonyl-CoA as a substrate [15] The gene encoding this bifunctional protein was named AUH (‘AU binding homolog of enoyl-CoA hydratase’) The RNAbinding activity of the ... (kcat ¼ 5.1) is favored by a factor of 20 over the dehydration reaction (kcat ¼ 0.26) which is consistent with the main role of AUH in leucine catabolism, the hydration of 3-MG-CoA Comparing the ... For the kinetic characterization of AUH described in the work at hand, AUH was overproduced in Escherichia coli as a maltose binding protein fusion (MBP-AUH) The complete AUH gene (producing...
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Báo cáo sinh học: " Characterization of vaccinia virus A12L protein proteolysis and its participation in virus assembly" pptx
Ngày tải lên :
18/06/2014, 18:20
... motif other than the AG/X sites in concert with involvement of another proteinase Given this atypical behavior, it is of interest to determine the essentiality of the A12L protein in viral replication ... the processing of other core proteins, which are completed within to hpi [7] The slow kinetics of the A12L cleavage event may be attributed to the possibilities of either inefficient processing ... proteins, p4b and p17K Similar to p4b processing, p17K was expressed in the presence and absence of rifampicin, whereas the smaller peptides were produced only in the absence of the drug, indicating that...
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Báo cáo hóa học: " Characterization of vaccinia virus A12L protein proteolysis and its participation in virus assembly" ppt
Ngày tải lên :
20/06/2014, 01:20
... motif other than the AG/X sites in concert with involvement of another proteinase Given this atypical behavior, it is of interest to determine the essentiality of the A12L protein in viral replication ... the processing of other core proteins, which are completed within to hpi [7] The slow kinetics of the A12L cleavage event may be attributed to the possibilities of either inefficient processing ... proteins, p4b and p17K Similar to p4b processing, p17K was expressed in the presence and absence of rifampicin, whereas the smaller peptides were produced only in the absence of the drug, indicating that...
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Báo cáo y học: "Phenotypic and genotypic characterization of Human Immunodeficiency Virus type 1 CRF07_BC strains circulating in the Xinjiang Province of China" pot
Ngày tải lên :
12/08/2014, 23:21
... characterize the genotype and phenotype of HIV-1 CRF07_BC strains circulating in Xinjiang province, in comparison with those of the subtype B' predominating in Anhui and Shanxi provinces In doing so, ... in gp120 of the CRF07_BC and subtype B' The frequency of potential N-linked glycosylation sites in the The frequency of potential N-linked glycosylation sites in the V1V2 region in gp120 of the ... CRF07_BC and subtype B' viruses The frequency of N- potential N-linked in the C2 region in The frequency of N- potential N-linked in the C2 region in gp120 of the CRF07_BC and subtype B' viruses There...
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Characterization of helicobacter pylori y glutamyl transpeptidase and its role in pathogenesis
Ngày tải lên :
12/09/2015, 09:55
... was increased in the presence of glutathione and glycyl-glycine, a GGT enhancer The results show the importance of GGT activity on the growth of H pylori DNA sequencing and the translated protein ... translated amino acid sequences of GGT proteins in different H pylori strains possessing different level of GGT activities Determining the subcellular localization of GGT in H pylori Examining the pathway ... of ammonia and indirectly by urease-induced stimulation of the inflammatory response, including recruitment of leukocytes and triggering of the oxidative burst in neutrophils Survey of Literature...
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Báo cáo " PROTECTING AGRICULTURAL CROPS FROM THE EFFECTS OF TROPOSPHERIC OZONE EXPOSURE: Reconciling Science and Standard Setting in the United States, Europe, and Asia" ppt
Ngày tải lên :
17/03/2014, 19:20
... However, studies conducted in the early 1980s in the United States and during the 1990s in Europe and other countries—including Japan, Pakistan, and Mexico—have indicated that many agricultural ... function of plant growth stage at the time of the excess O3 The level approach would include consideration of parameters that in uence the flux of O3 into the plant and which are critical in converting ... at the individual plant and leaf level in order to explain the variation in O3-dose/yieldreduction–response relationships There is, however, an increasing interest in better understanding the...
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Strengthening country office capacity to support Sexual and reproductive health in the new aid environment docx
Ngày tải lên :
28/03/2014, 16:20
... diversity, and a health landscape that is increasingly crowded in terms of actors and issues, have increased the challenge of maintaining the profile of SRH in sectoral and national planning processes, ... Country offices are recognizing the opportunities that the MDG5 focus brings, and seeking to map out the remaining elements of SRH into the matrix of planning processes Family planning can be located ... out the changes in the aid environment experienced since the first case-studies were undertaken, the perceptions of UNFPA and WHO country office staff of their understanding of these changes, and...
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Báo cáo y học: "The abilities of improved schizophrenia patients to work and live independently in the community: a 10-year long-term outcome study from Mumbai, India" ppsx
Ngày tải lên :
08/08/2014, 23:21
... cities The social determinants of health have been well established [4] However, a better understanding of the impact of these factors on outcome of schizophrenia is needed Life in Mumbai, India, the ... admission into long-term care facilities This indicates that 107 out of 122 available patients (87.7%) were living in the community with their families The remaining 78 patients (39%) out of initially ... that the numerical rate of outcome is 30.5% but 27.1% of patients remain dependent on others for living and 60% of patients not succeed in gainful employment The second and more satisfying perspective...
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Báo cáo nghiên cứu khoa học " Development of Modeling System to Simulate Hydrodynamic and Environmental Quantities in the Hai Phong Estuary, Vietnam " pot
Ngày tải lên :
09/08/2014, 18:20
... because the storm surge inundates low lands, erodes beaches, and increases the salinity of rivers and estuaries in the storm or typhoon season The extent and severity of storm impact has recently increased ... Variation of the volumetric concentration [m /m ] in offshore of the Cathai island (water depth = 3.6 m) and near the Cathai island (water depth = 2.1 m) for storm wind speed of 18 m/s (serie 1) and ... investigates a hindcasting of water level during a storm surge in the Gulf of Tonkin The Do Son beach area is of special interest because the coast suffered great losses from several typhoons in the past...
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Báo cáo y học: "Enhanced glutamate, IP3 and cAMP activity in the cerebral cortex of Unilateral 6-hydroxydopamine induced Parkinson’s rats: Effect of 5-HT, GABA and bone marrow cell supplementation" ppsx
Ngày tải lên :
10/08/2014, 05:21
... http://www.jbiomedsci.com/content/18/1/5 by linear regression analysis by plotting the specific binding of the radioligand on the X-axis and bound/free on the Y-axis The maximal binding is a measure of the total number of receptors ... present in the tissue and the equilibrium dissociation constant is the measure of the affinity of the receptors for the radioligand The Kd is inversely related to receptor affinity Quantification of ... Analysis of the Receptor-Binding Data Linear Regression Analysis for Scatchard Plots The data were analysed [25] The specific binding was determined by subtracting non-specific binding from the total...
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Báo cáo y học: "Development of daily rhythmicity in heart rate and locomotor activity in the human fetus" ppt
Ngày tải lên :
10/08/2014, 09:20
... prevalent during "quiet" hours as during "active" hours Statistical processing of the findings regarding the duration of each type of the oscillations and "active" and "quiet" hours of the fetus ... done according to the following parameters: hourly fluctuations in maternal and fetal HR, intra-minute fluctuation of HR, and baseline rhythm The baseline was defined as the level obtained at a.m., ... mm/sec The recordings obtained were processed by the calculation of maternal and fetal heart rate (FHR) in 5-minute intervals and transferred onto the cardiotachogram representing a graph of intra-minute...
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báo cáo khoa học: " Years of life lost to prison: racial and gender gradients in the United States of America" pps
Ngày tải lên :
11/08/2014, 18:20
... differences in the number of years of life lost to imprisonment in the United States population by gender and race There was on average 1.75 million persons between the ages of 18 and 44 in prison in the ... http://www.harmreductionjournal.com/content/5/1/4 dividing the years lived in each age range of the life table into these two states using prevalence of imprisonment by gender and race These figures were then used to compute the life table ... criminalizing drug possession are the major driver of the imprisonment of the nonviolent offenders in the United States, changes in these laws could be expected to have a significant impact Other...
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Báo cáo y học: "Evaluation of the effects of a VEGFR-2 inhibitor compound on alanine aminotransferase gene expression and enzymatic activity in the rat liver" pdf
Ngày tải lên :
13/08/2014, 13:20
... distribution of drugs and toxins may help in understanding the potential localization of hepatic diseases and carcinomas within the liver Understanding these regional effects is critical in the interpretation ... differential in nature The distributional effect of copper, for example is thought to reflect the site of gastrointestinal absorption and portal streamlining into the liver [14] Other studies have indicated ... whether the chronic effect of AG28262 will result in morphologic changes or if the compound will independently alter the intrinsic regulation of ALT gene expression and synthesis Further investigation...
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Báo cáo y học: "Toxicogenomic analysis of Caenorhabditis elegans reveals novel genes and pathways involved in the resistance to cadmium toxicity" potx
Ngày tải lên :
14/08/2014, 07:21
... growth/development) using the COPAS Biosort [21] Inhibiting the expression of these genes resulted in different degrees of slow growth in the presence of cadmium, compared to the RNAi control in the same ... enzyme involved in the biosynthesis of retinoic acid Cadmium has been shown to act synergistically with retinoic acid in the induction of limb-bud malformation in mice [51] The Y46G5A.24 network includes ... unknown To investigate the relationship between cadmiuminduced gene expression and resistance to cadmium toxicity, the effects of inhibiting the expression of the cadmiumresponsive genes in the presence...
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Báo cáo y học: "Molecular basis of telaprevir resistance due to V36 and T54 mutations in the NS3-4A protease of the hepatitis C virus" pdf
Ngày tải lên :
14/08/2014, 08:20
... shows the relevance of V55 regarding the structural integrity of the catalytic site of the protease This finding can readily be explained by the smaller size of the side chain of alanine in contrast ... placement of the cyclopropyl group of CPX in 1RTL The cyclopropyl group is buried in the surface cavity and faces towards the aromatic ring of F43 The binding modes of the 1RTL and 2FM2 ligands CPX and ... 2) T54 is at the very end of the strand β3, next to a loop directly involved in the ligand binding cavity at the protein surface The side chains of V36 and T54 point towards each other We identified...
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Báo cáo y học: "Characterization of Human Erythrocytes as Potential Carrier for Pravastatin: An In Vitro Study"
Ngày tải lên :
25/10/2012, 11:10
... pravastatin to the total amount of that added during the entire loading process Cell recovery The percentage ratio of the hematocrite value of the final loaded cells to that of the initial packed ... of PBS containing the known concentration of the drug and 2.5 mmol of ATP, 2.5 mmol MgCl2 and 2.5 mmol of CaCl2, gently mixing to avoid hemolysis and incubation for 15 http://www.medsci.org Int ... including inhibition of the metabolism [5], administration of statins with certain juices [6] or inhibition of Pgp [3] Unfortunately these strategies are frequently associated with increase the...
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Tài liệu Báo cáo khoa học: Characterization of chitinase-like proteins (Cg-Clp1 and Cg-Clp2) involved in immune defence of the mollusc Crassostrea gigas docx
Ngày tải lên :
19/02/2014, 00:20
... based on the conserved amino acid sequences of the catalytic domain of members of the GH18 family resulted in the amplification of an expected 147 bp sequence Cloning and sequencing of this fragment ... composed of the sole Glyco_18 domain, the C-terminal tail of C gigas CLPs may not noticeably contribute to the structure and the function of these proteins Interestingly, Cg-Clp1 and Cg-Clp2 C-terminal ... from the oyster Crassostrea gigas [5] Interestingly, this protein, named C gigas chitinase-like protein (CgClp1) was found to be involved in the control of growth and remodelling processes in a...
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Báo cáo khoa học: Kinetic and mechanistic characterization of Mycobacterium tuberculosis glutamyl–tRNA synthetase and determination of its oligomeric structure in solution pptx
Ngày tải lên :
07/03/2014, 03:20
... Fig pH dependence of the steady-state kinetic parameters kcat and kcat KL-Glu of Mt-GluRS The apparent kcat (in min)1) and kcat KL)1 )1 Glu (in ặmM ) values of the reaction catalysed by Mt-GluRS ... rationale for the discriminating behaviour of Mt-GluRS in E coli Studies on Tt-GluRS indicated that discriminating and nondiscriminating GluRS can be distinguished on the basis of the presence of a specic ... except that the concentration of one of the substrates was varied and the levels of the others xed In these assays the amount of enzyme was chosen in order to observe linearity up to 10 min, and aliquots...
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Báo cáo khoa học: Engineering and characterization of human manganese superoxide dismutase mutants with high activity and low product inhibition potx
Ngày tải lên :
07/03/2014, 11:20
... drop in the extent of product inhibition, the Gln143 residue was also found to be critical for catalytic activity In all the attempted replacements of Gln143, the catalytic activity (kcat) of the ... some of the catalytic activity lost by the imposition of the Q143A substitution These mutations are subsequently kinetically characterized separately and in combination in the context of the parental ... selecting mutants with increased catalytic activity from a large library of hMnSOD variants In particular, the application of the random mutagenesis methods of error-prone PCR and DNA shuffling to the...
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Báo cáo khoa học: Identification and characterization of 1-Cys peroxiredoxin from Sulfolobus solfataricus and its involvement in the response to oxidative stress pdf
Ngày tải lên :
07/03/2014, 12:20
... clarified in detail and could play a key role in the detoxification processes In this study we examined the role of Bcp2 in order to increase the knowledge of the enzymatic activity involved in the ... Furthermore, we report the cloning, the expression and the characterization of the recombinant protein rBcp2 in order to shed light on its role in the detoxification process and on its catalytic mechanism ... [9] The main characteristic of 2-Cys Prxs, the largest Prx class, is their second redox-active cysteine, the resolving cysteine (Cys-SR) In typical 2-Cys, Sp at the N terminus and SR at the C...
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