integrin αvβ3 during bovine fertilization in vitro
Tài liệu Báo cáo khoa học: Template requirements and binding of hepatitis C virus NS5B polymerase during in vitro RNA synthesis from the 3¢-end of virus minus-strand RNA docx
... domain in the 3¢-end of the HCV minus-strand RNA inhibits in vitro initiation of RNA synthesis by the viral polymerase [19] Kashiwagi et al [20] studied RNA synthesis by recombinant HCV NS5B using ... synthesized in one round of synthesis, we performed RdRp assays in the presence of heparin in order to prevent reinitiation In a first step we determined the heparin concentration needed to prevent reinitiation ... radioactivity incorporated into the nucleic acids was measured after TCA precipitation and plotted against the incubation time in minutes the 3¢X 98 nt As shown in Fig 6A, in the absence of heparin this...
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Báo cáo y học: " Functional inhibition of NF-κB signal transduction in αvβ3 integrin expressing endothelial cells by using RGD-PEG-modified adenovirus with a mutant IκB gene" ppsx
... virus is αvβ3 integrin specific [15] This specificity furthermore means that in vivo, Available online http://arthritis-research.com/content/8/1/R32 αvβ3 integrin- positive cells, including angiogenic ... with αv integrin specific infectivity and transgene expression We next confirmed the change in the entry pathway of RGDretargeted adenovirus into HUVECs from a CAR-dependent to an αvβ3 integrin- dependent ... to the initiation of angiogenesis [4] In the current study, we show that adenoviral vectors encoding dnIκB protein modified to selectively infect pro-angiogenic, αvβ3 integrin expressing endothelial...
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Báo cáo sinh học: " The incidence of polyploidy and mixoploidy in early bovine embryos derived from in vitro fertilization" ppt
... aberrations (including haploid and polyploid cells) in isolated inner cell mass (ICM) from bovine blastocysts cultured either in vivo or in vitro was quite high Moreover the incidence of embryos carrying ... h incubation oocytes were washed and placed into fertilization droplets (10 oocytes in 50 wL droplet) overlaid by paraffin oil (Merck) Before insemination, hypotaurine (4.6 pg/mL), epinephrine ... S, Nakahara T (1990a) Cell number and incidence of chromosomal anomalies in bovine blastocysts fertilized in vitro followed by culture in vitro or in vivo in rabbit oviducts Theriogeneology 33,...
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Báo cáo y học: "A cyclic-RGD-BioShuttle functionalized with TMZ by DARinv “Click Chemistry” targeted to αvβ3 integrin for therapy"
... antibodies to αvβ3 integrin and its antagonists like 327 arg-gly-asp (RGD)-containing peptides, including osteopontin, bone sialoprotein, vitronectin and fibrinogen are considered as efficient inhibitors ... inhibiting features of RGD-containing peptides against αvβ3 integrin expressing cells [18] whereas the activity of αvβ5 integrin was already documented from the Boulanger and Nemerow groups in ... expressing αvβ3 integrin In comparison with the normal expression level of MCF-7 mammary cancer cells, αvβ3 low expressing HeLa cervix cancer cells were examined, which express the αvβ5 integrin...
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Tài liệu Báo cáo Y học: Proteolysis of bovine b-lactoglobulin during thermal treatment in subdenaturing conditions highlights some structural features of the temperature-modified protein and yields fragments with low immunoreactivity pptx
... beta-lactoglobulin and its similarity to plasma retinol-binding protein Nature 324, 383–385 Pervaiz, S & Brew, K (1985) Homology of beta-lactoglobulin, serum retinol-binding protein and protein HC Science ... monomers obtained at low pH [35] or under pressure [10] Arg148 is not the only buried arginine in the structure of BLG The whole side chain of Arg40 (the other main point of trypsin action) in the ... concentration of TP1 remained constant during progressive hydrolysis of heated BLG by trypsin, whereas the concentration of the intact protein decreased with an accompanying increase in TP2 The pattern...
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Tài liệu Báo cáo Y học: Progressive apoptosis in chorion laeve trophoblast cells of human fetal membrane tissues during in vitro incubation is suppressed by antioxidative reagents pptx
... TNFa-induced U937 cell apoptosis by maintaining mitochondrial integrity and function [43], and ricin-induced apoptosis of U937 cells by maintaining an intracellular reducing condition by acting ... chorion laeve tissue during in vitro incubation Tissue was incubated in medium containing mM PDTC (A), 30 mM NAC (B), mM NDGA (C) or mM alloprinol (D) the upper and lower parts in each figure show ... mRNAs containing Mn-SOD increase to counterbalance these phenomena The induction and increase of oxidative stress during pregnancy has been indicated by increasing lipid peroxidation in pregnant...
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In Vitro Fertilization – Innovative Clinical and Laboratory Aspects Edited by Shevach Friedler potx
... urinary human chorionic gonadotropin in inducing final follicular maturation and ovulation in in vitro fertilization 14 In Vitro Fertilization – Innovative Clinical and Laboratory Aspects procedures: ... LH receptors during the intermediate follicular phase (Fillicori et al., 2003 a,b) Gonadotropin releasing hormone In Vitro Fertilization – Innovative Clinical and Laboratory Aspects In our research, ... releasing IGF (insulin-like growth factor) IGF-II has a major role in implantation and trophoblast invasion (Giudice et al., 2002) Insuline-like growth factors can bind to six IGFbinding proteins,...
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Báo cáo khoa học: NovelN,N¢-diacyl-1,3-diaminopropyl-2-carbamoyl bivalent cationic lipids for gene delivery – synthesis,in vitro transfection activity, and physicochemical characterization docx
... and maintained in serum medium (Dulbeccos modied Eagles medium supplemented with 10% fetal bovine serum, 50 unitsặmL)1 penicillin and 50 lgặmL)1 streptomycin) After incubation for h at 37 C in a ... there was a reduction in plasmid compaction efciency with increasing hydrophobic chain length Interestingly, the transfection-inactive lipid 1,3lb1 was the most efcient at condensing pDNA, with complete ... 7D), suggesting a minimal inuence of electrostatic interactions on cell internalization of 1,3lbpDNA complexes in vitro 40 30 20 10 1,3lb1 1,3lb2 1,3lb3 1,3lb4 1,3lb5 Fig Lipid mixing, as assessed...
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Báo cáo khoa học: The C-terminal region of the proprotein convertase 1⁄ 3 (PC1⁄ 3) exerts a bimodal regulation of the enzyme activity in vitro pdf
... N-terminal domains, thus rendering intriguing the possibility that PC1 ⁄ CT-peptide might by itself possess intrinsic inhibiting properties Production of recombinant CT-peptide We expressed in bacteria ... tethering, thus insuring cell-surface anchoring [37] In other cases, the CT-peptide contains integral transmembrane motifs affecting the sorting and recycling of furin and PC5 ⁄ 6B [38–40] In the case ... purification of recombinant mPC1 ⁄ and hfurin Recombinant murine PC1 ⁄ was produced using the baculovirus expression system in Sf9 insect cells [7] or through intracoelemic injection in insect larvae...
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IEC 60909 3 short circuit currents in three phase a c systems currents during two separate sim
... earthing impedance of a station according to Equation (16) Z ETtot Total earthing impedance of the short circuited tower according to Equation (21) ZP Driving point impedance of an infinite chain ... on the following documents: FDIS Voting report 73/127/FDIS 73/128/RVD Full information on the voting for the approval of this standard can be found in the report on voting indicated in the above ... current I EB for a line-to-earth short circuit occurring on the overhead line in the vicinity of station B may be higher than I Etot for a line-to-earth short circuit inside station B in Figure (see...
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Báo cáo sinh học: " In vitro permissivity of bovine cells for wild-type and vaccinal myxoma virus strain" pot
... http://www.virologyj.com/content/4/1/94 information concerning interactions between MYXV and bovine cells is available yet In this study, we characterized the infection of bovine cell lines and bovine peripheral blood ... comparing two different MYXV strains (a wild-type strain (T1) and a cell-cultured attenuated vaccinal strain (SG33) [14]) we verified the stability of the viral tropism in vitro Findings Three bovine ... KOP-R P3 BT MDBK Figure Permissivity of bovine cell lines for myxoma virus Permissivity of bovine cell lines for myxoma virus Bovine cells were maintained in DMEM (KOP-R and BT) or MEM (MDBK) supplemented...
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báo cáo hóa học: " Interleukin-1 beta and neurotrophin-3 synergistically promote neurite growth in vitro" pot
... and infarct volumes after ischemic brain damage [4] and direct application of the recombinant cytokine results in an enhanced infarct volume [5] In traumatic brain injury, antibodies against ... organotypic brain slices, also increasing the number of slices displaying maximal outgrowth This is in contrast to several single cell studies in which neurotrophins are highly efficient in promoting axonal ... neuronal survival in the CNS [10, 11] This has also been observed in axonal growth in the peripheral nervous system both in vivo following sciatic nerve injury [12, 13] and in vitro in adult dorsal...
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Báo cáo hóa học: " In vitro permissivity of bovine cells for wild-type and vaccinal myxoma virus strains" potx
... http://www.virologyj.com/content/4/1/94 information concerning interactions between MYXV and bovine cells is available yet In this study, we characterized the infection of bovine cell lines and bovine peripheral blood ... comparing two different MYXV strains (a wild-type strain (T1) and a cell-cultured attenuated vaccinal strain (SG33) [14]) we verified the stability of the viral tropism in vitro Findings Three bovine ... KOP-R P3 BT MDBK Figure Permissivity of bovine cell lines for myxoma virus Permissivity of bovine cell lines for myxoma virus Bovine cells were maintained in DMEM (KOP-R and BT) or MEM (MDBK) supplemented...
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báo cáo hóa học:" In vitro suppression of the MMP-3 gene in normal and cytokine-treated human chondrosarcoma using small interfering RNA" doc
... cell death Proteinases or inappropriate ECM molecules induce apoptosis of mammary epithelial cells in culture, presumably through altered signaling from integrins [37] Moreover, in the present ... have definite effects on NO production in the joints These inhibitory effects of HA on cell apoptosis may play a role in its mechanism of action in chondroprotection [38] Our findings indicated ... and purification in each group was performed using an RNeasy Mini Kit protocol (Qiagen), including the DNA removal step, according to the manufacturer's guidelines RNA was eluted in 40 μl of RNasefree...
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báo cáo hóa học:" Activation of PKA, p38 MAPK and ERK1/2 by gonadotropins in cumulus cells is critical for induction of EGF-like factor and TACE/ ADAM17 gene expression during in vitro maturation of porcine COCs" doc
... expression in cumulus cells cultured with FSH and/or LH have remained unclear during in vitro maturation of porcine COCs http://www.ovarianresearch.com/content/2/1/20 The binding of FSH and/or LH in ... mRNA expression during in vitro maturation of porcine COCs COCs were cultured with FSH, LH and/or PKA inhibitor (H89), p38 MAPK inhibitor (SB203580), MEK inhibitor (U0126) or PI3K inhibitor (LY294002) ... ovulation in mouse [9-11] Since the suppression of the EGF receptor tyrosine kinase activity by specific inhibitor completely suppressed cumulus expansion and meiotic maturation of oocytes during in vitro...
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IN VITRO FERTILIZATION – INNOVATIVE CLINICAL AND LABORATORY ASPECTS pdf
... urinary human chorionic gonadotropin in inducing final follicular maturation and ovulation in in vitro fertilization 14 In Vitro Fertilization – Innovative Clinical and Laboratory Aspects procedures: ... LH receptors during the intermediate follicular phase (Fillicori et al., 2003 a,b) Gonadotropin releasing hormone In Vitro Fertilization – Innovative Clinical and Laboratory Aspects In our research, ... releasing IGF (insulin-like growth factor) IGF-II has a major role in implantation and trophoblast invasion (Giudice et al., 2002) Insuline-like growth factors can bind to six IGFbinding proteins,...
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Báo cáo khoa học: Ảnh hưởng của bình nuôi, thế hệ cây vụ thiosulfat bạc đến sinh trưởng, hệ số nhân vµ chất lượng cây khoai tây in vitro pot
... Minh1 SUMMARY The disease-free in vitro materials of two potato cultivars, Mariella and Diamant were used for culture in plastic box, nylon bag, Erlen-meyer flask and cylinder flask The in vitro ... bạc đến sinh trởng, hệ số nhân chất lợng khoai tây in vitro Effects of culture containers, plant generation and silver thiosulphate on the growth, multiplication rate and quality of in vitro potato ... Sự sinh trởng phát triển khoai tây in vitro loại dụng cụ nuôi cấy khác đợc thể bảng cho thấy: khả sử dụng hộp nhựa, bình trụ để nuôi hoàn toàn đợc sai khác tiêu sinh trởng, phát triển in vitro...
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BÀI 3: GIEO HẠT IN-VITRO I.CHỌN MÔ CẤY VÀ XỬ LÝ MÔ CẤY pps
... - Tủ cấy (laminar) - Tủ lạnh - Cân kỹ thuật - Máy cất nước lần - Máy khuấy từ - pH kế 2.2.3 Hoá chất - Dung dịch Skoog I, II III môi trường MS - Stock vitamin Morel - Stock glycin - Đường saccharose ... cho sinh viên làm quen với thao tác vô trùng mẫu cấy kỹ thuật cấy vô trùng 2.2 Dụng cụ, thiết bị hóa chất 2.2.1 Dụng cụ: - Bình tam giác (250mL) - Giấy cấy vô trùng - Bécher (cốc đốt thuỷ tinh) ... 1L môi trường) - Skoog I (MS):100mL - Skoog II (MS): 2mL - Skoog III (MS): 5mL - Vitamin Morel: 2mL - Glycine: 2mL - Sucrose: 30g - pH :5,8 - Agar:7g 2.3.2 Nguyên liệu thực vật: - Các hạt cam...
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Giáo trình : Kỹ thuật nhân giống in vitro part 3 ppsx
... pyridoxine, thiamin, glycine, fonic acid v biotin + Casein hydrolysate, D-Ca- pantothenate, choline chloride, cysteine, malic acid, ascorbic acid, adenine sulfate, riboflavin v glutamine: cú ... qu v theo dừi biu hin ca gen bin np Protoplast sau tỏch T bo ging ,loi B T bo ging, loi A T bo lai soma mang c ca c hai ging, loi A v B 25 Lớp Cây trồng 49C - Nhóm Công nghệ sinh học nông nghiệp ... Bo qun ht kho lnh to thnh seed bank + Bo qun in vitro to thnh in vitro bank + Bo qun ADN to thnh DNA bank Trong bn phng thc trờn, phng thc bo qun in vitro cú vai trũ quan trng vỡ bo qun mt khụng...
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