integer complex and character
Tài liệu Báo cáo khoa học: Spectroscopic characterization of a higher plant heme oxygenase isoform-1 from Glycine max (soybean) ) coordination structure of the heme complex and catabolism of heme docx
... (A) and (B) and 0.2 mW for (C)–(E); field modulation frequencies, 100 kHz; field modulation amplitude, 10 G for (A) and (B) and G for (C)–(E); signal acquisition temperature, K for (A) and (B) and ... asymmetrically broadened Soret band with the blue-shifted maximum at around 390 nm, compared with the Soret-band features of the wild-type complex, and no other characteristic bands at the visible region ... features of the heme–GmHO-1 complex and of the mechanism of heme degradation were performed, and the results were compared with those for the heme complexes of SynHO-1 and rHO-1 We found that, in...
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Tài liệu Báo cáo khoa học: Purification and characterization of a membrane-bound enzyme complex from the sulfate-reducing archaeon Archaeoglobus fulgidus related to heterodisulfide reductase from methanogenic archaea pdf
... the a band at 557 nm absorption maxima at 420 nm (c band), 530 nm (b band) and 557 nm (a band) are characteristic of cytochrome b Heme was extracted from the protein with acidic acetone and the ... transmembrane helices Catalytic properties of the Hme complex and characterization by UV/Vis spectroscopy To determine whether the cytochrome present in the Hme complex is reduced by menaquinone, in vitro ... molecular mass of 53 kDa appears as a double band in unboiled samples (lanes A1 and B1) Identification of the genes encoding the subunits of the Hme complex and sequence analysis The N-terminal sequences...
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Báo cáo khoa học: The 2-oxoacid dehydrogenase multi-enzyme complex of the archaeon Thermoplasma acidophilum ) recombinant expression, assembly and characterization docx
... 2-oxoacids and 2-Oxoacid dehydrogenase complex from the Archaea pyruvate [14] In the current paper, we report the cloning and expression of the E2 and E3 genes of the same operon from T acidophilum, and ... Relative molecular mass of the E2 core and the assembled complex Analytical centrifugation and DLS were used to determine the Mr of both the E2 core and the assembled complex E2 core Sedimentation velocity ... mM MgCl2 and 0.2 mM TPP (A) Gel filtration of the assembled complex was carried out at 25 °C as described in Experimental procedures, and the fractions were assayed for whole complex, E1 and E3...
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![Báo cáo khoa học: Recognition of DNA modified by trans-[PtCl2NH3(4hydroxymethylpyridine)] by tumor suppressor protein p53 and character of DNA adducts of this cytotoxic complex potx](https://media.store123doc.com/images/document/14/rc/cf/small_cfu1394954409.jpg)
Báo cáo khoa học: Recognition of DNA modified by trans-[PtCl2NH3(4hydroxymethylpyridine)] by tumor suppressor protein p53 and character of DNA adducts of this cytotoxic complex potx
... the bands corresponding to single strands of DNA and interstrand cross-linked duplex were quantified Rearrangement of intrastrand cross-links The platinated oligodeoxyribonucleotide (top strand ... activity with the cis counterpart complex, but this work does not aim to synthesize and characterize a new compound and to investigate its cytotoxicity Thus, to expand the database of biochemical ... Cytotoxicity and antitumor activity of bis (platinum) complexes – a novel class of platinum complexes active in cell lines resistant to both cisplatin and 1,2-diaminocyclohexane complexes J Med...
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Báo cáo khoa học: Expression and characterization of cyanobacterium heme oxygenase, a key enzyme in the phycobilin synthesis Properties of the heme complex of recombinant active enzyme potx
... Oligo I and Oligo II were inserted between the sites of NdeI and SpeI; Oligo III, between the sites of SpeI and SacI; Oligo IV and Oligo V, between SacI and AvrII; Oligo VI, between AvrII and ClaI; ... Soret bands of the ferric and ferrous forms, having maxima at 402 and 427 nm, respectively, are slightly blue-shifted compared with those of mammalian (404 and 431 nm) and bacterial (404, 406 and ... [23], and Hem O, N meningitidis A 2855 [25] Soret and Q (a and b) bands of the oxy form and the CO-bound form not show specific differences from those of mammalian or bacterial HOs The small band...
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Báo cáo y học: "The propofol infusion syndrome: more puzzling evidence on a complex and poorly characterized disorder" ppt
... prolonged periods of time (typically days) Wysowski and Pollock analyzed the US Food and Drug Administration’s Adverse Event Reporting System database and identified 68 adult patients who died after ... administration of propofol in higher doses and usually for longer durations greatly increases the risk of PRIS in both children and adults It is conceivable that Roberts and colleagues failed to detect ... of impaired muscular and myocardial free fatty acid utilization [10] It is therefore remarkabe that rhabdomyolysis was not observed in a single case in the study by Roberts and colleagues Brugada-like...
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Isolation and characterization of an extracellular polymer closely related to flocculation of activated sludge
... Water and Environment Technology, Vol.3, No.2, 2005 viscous in solution, are mainly composed of polysaccharides, although proteins and nucleic acids from cell autolysis may be present (Stoveland and ... kaolin clay and activated charcoal flocculated satisfactorily (the flocculation values at pH 2.5 of kaolin clay and activated charcoal were 79.6% and 67.1%, respectively) On the other hand, in the ... for the study of floc strength and reflocculation of activated sludge Environ Tech Lett., Vol.2, 579-588 Kurane, R Takeda, K and Suzuki, T (1986) Screening for and characteristics of microbial...
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Preparation and characterization of the PVDF-based composite membrane for direct methanol fuel cells
... (DMAc) firstly, and then styrene, divinylbenzene (DVB) and benzoyl peroxide (BPO) were added to the solution and stirred with water bath at 343 K The dosage of PVDF, styrene, DVB and BPO was that ... polymerization and phase inversion, and all of the other reagents were analytical grade 2.2 Preparation of the PVDF-based composite membrane The PVDF powders were basified firstly, and the double ... immersed in deionized water for 48 h, and then the sulfonated copolymer washed with deionized water and dried for usage The sulfonated copolymer with weight of 15 g and 2.5 g polyvinyl pyrrolidine...
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Tài liệu RECEIVER TYPES AND CHARACTERISTICS doc
... Good POI High complexity; new technology Table Receiver Types vs Signal Types Receiver Type Signal Type Wide-Band Crystal Video TRF Crystal Video IFM Narrow-Band Superhet Wide-Band Superhet Channelized ... Receiver Type Feature Wide-Band Crystal Video TRF Crystal Video IFM Narrow-Band Superhet Wide-Band Superhet Channelized Microscan Acousto-optic Instantaneous Analysis Bandwidth Very wide Narrow ... Very complex, diverse systems required, at least aircraft Can support weapon delivery position requirements High quality receivers, DME (3 sites) very wideband data link Very rapid, can handle...
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Design, fabrication, and characterization of a solenoidsystem to generate magnetic field for an ECR proton source
... conductor, water pressure drop and flow, the inlet and outlet connections were provided Total five inlet and five outlet connections were provided The water flow rate liter/min and pressure 3·5 kg/cm2 was ... M, Ando L, Guo X H, Gobin R, Ferdinand R 2004 Status of the Trasco intense proton source and emittance measurements Rev Sci Instrum 75(5): 1423–1426 Ciavola G, Celona L, Gammino S, Presti M, Ando ... field, and (b) flat magnetic field are shown in figure The calculation was also performed analytically (Montgomery 1966) using standard relations for calculating magnetic field Design, fabrication, and...
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Tài liệu Báo cáo " Preparation and characteristics of LiF:Mg,Cu,Na,Si thermoluminescent material " pptx
... activated with Mg, Cu, Na and Si and studied the dependence of the glow curve and the TL intensity on thermal treatment and the concentration of the dopants The LiF:Mg,Ti and LiF:Mg,Cu,P powders ... CuCl2 , and (NH4 )2 H2PO4 with required concentration were added to LiF precipitated by reaction (1) The material obtained was washed, dried, and was annealing in an oven at 700 o C for hour, and ... incorporated The material precipitated was filtered, washed, dried, and was subjected to thermal treatment in an oven at 640o C for hour, and then was annealed at 400o C for hour LiF:Mg,Cu,P powders...
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Tài liệu Báo cáo "Preparation and characterization of nanocomposite TiO2/SnO2 films " pdf
... impregnated and annealed at 325, 375 and 425oC Discussion TiO2 belongs to dielectric materials With a wide band gap of 3.2 eV, at room temperature there are no free carriers in the conducting band Under ... be excited, some photo electrons jumped to conducting band and can take part in the electric conduction However the systems of high energy band gap having a tendency to produce high potential barrier ... increases according to time and temperature of annealing In the other hand, SnO2 is self-doping semiconductor Due to the stoichiometric deviation, some Sn atoms were formed and played a role of the...
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Tài liệu GLOBAL PHYLOGEOGRAPHY OF A CRYPTIC COPEPOD SPECIES COMPLEX AND REPRODUCTIVE ISOLATION BETWEEN GENETICALLY PROXIMATE ‘‘POPULATIONS’’ ppt
... populations in Lakes Ohnuma and Akanko (sites 29 and 30) were thought to have been introduced from the island of Honshu in Japan (Ban and Minoda 1989) Morphological Stasis and Plasticity For the copepod ... regions of 16S rRNA; (2) a combined dataset of 16S rRNA and COI; and (3) first, second, and third codon positions of COI For 16S rRNA, tests on stem and loop regions were performed on 15 E affinis populations ... Bothnia; (33) Gulf of Finland; (34) Sallvik Fjord, Finland; ¨ (35) Baltic Sea Proper; (36) IJsselmeer, Netherlands; (37) Gironde estuary, France; (38) Tamar estuary, England ence among the non-European...
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Tài liệu Báo cáo khoa học: Identification and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebrafish, Danio rerio docx
... t-bet, stat6 and foxp3 were also determined and found to be very similar to their human homologues Both the human and zebrafish T-bet and Foxp3 genes contained the same number of exons and introns ... in the lung tissue of mouse and spleen and thymus of human and mouse [11,37] Foxp3 is also highly expressed in lymphoid organs, such as the thymus and spleen [49], and its expression within a ... STAT protein DNA-binding domain and SH2 domain of stat6, and the zinc-finger domain, leucine-zipper domain and fork-head domain of foxp3 In addition, for stat6 and foxp3, there were a few other...
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Tài liệu Báo cáo khoa học: Molecular cloning and characterization of soybean protein disulfide isomerase family proteins with nonclassic active center motifs pdf
... (Fig 1D) GmPDIL-3a and GmPDIL-3b have two predicted thioredoxin domains between amino acids 65–164 and 403–481, and 68–167 and 406–508, respectively, corresponding to the a-domain and a¢-domain of ... (Fig 3A, lanes and 5) Anti-GmPDIL-3a serum reacted with both a 55 kDa and a 59 kDa band in western blot analysis of cotyledon proteins (Fig 3A, lane 3) Both the 55 and 59 kDa bands were N-glycosylated, ... left, and density (gÆmL)1) is indicated on the top (B) Microsomes were treated without (lanes and 2) or with (lanes and 4) proteinase K, in the absence (lanes and 3) or presence (lanes and 4)...
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Tài liệu Báo cáo khoa học: Identification and characterization of an R-Smad ortholog (SmSmad1B) from Schistosoma mansoni pdf
... and translated into a 380 amino acid SmSmad1B protein (bottom) Regions encoding MH1, linker and MH2 are in light gray, gray and dark gray, respectively, and the regions representing the 5¢- and ... SmSmad2 and SmSmad4 and resulted in the formation of a stable and functional Smad complex [6,7] The above results are consistent with the report that human GCN5 interacts with both TGFb- and BMP-related ... snail), cercariae, 3-day-old and 7-day-old cultured schistosomules, 15 day, 21 day, 28 day and 35 day parasites, adult worm pairs, separated adult female and male worms, and eggs cDNA from uninfected...
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Tài liệu Báo cáo khoa học: Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds docx
... numerals I, II, III and IV and refers to each pulchellin Characterization of four pulchellin isoforms isoform (P I, P II, P II and P IV) The A-chain of P II was formerly cloned and named recombinant ... (types A+, B+ and O+), rabbit and horse where they showed blood group specificity and distinct hemagglutination activity P I and P II promoted hemagglutination of human erythrocytes at 22.5 and 27.5 ... data-dependent mode, Characterization of four pulchellin isoforms and multiplycharged peptide ions (+2 and +3) were automatically mass selected and dissociated in MS ⁄ MS experiments Typical LC and ESI conditions...
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Tài liệu Báo cáo khoa học: Production and characterization of a secreted, C-terminally processed tyrosinase from the filamentous fungus Trichoderma reesei ppt
... Stereospecificity and substrate specificity The stereospecificity of T reesei TYR2 was studied by following the activities on 15 mm l-dopa, dl-dopa and d-dopa and 2.5 mm l-dopa, dl-dopa and d-tyrosine, and the ... Tyrosinase enzymes and their genes have previously been characterized from bacteria, fungi, plants and mammals The most extensively investigated fungal tyrosinases, from both a structural and a functional ... 9.5) [51] and pine needle tyrosinase (9–9.5) [52] Many fungal tyrosinases have their pH optima at neutral and slightly acidic pH, e.g N crassa and Aspergillus flavipes at pH 6.0–7.0 [53,54] and Pycnoporus...
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Tài liệu Báo cáo khoa học: Cloning and characterization of CBL-CIPK signalling components from a legume (Pisum sativum) ppt
... treatment of pea seedlings were cold (A and B), salinity (C and D), wounding (E and F), drought (G and H), SA (I and J), ABA (K and L) and calcium (N and O) Panel M is the control for ABA responsive ... Figure 7A, D, I and L are diamidino-2phenylindole hydrochloride (DAPI) stained cells showing the nucleus, and Fig 7C, F, K and N are the merged images of A and B, D and E, I and J, and L and M, respectively ... canonical EF hand The amino acid D at position 1, of EF1 is replaced by amino acid A (Fig 1B) The EF1 and EF2 are 22 amino acids apart, whereas EF2 and EF3, and EF3 and EF4 are 25 and 32 amino...
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Tài liệu Báo cáo khóa học: Cloning and characterization of two distinct isoforms of rainbow trout heat shock factor 1 ppt
... amplification cycles of 0.5 at 94 °C, 0.5 at 50 °C and 0.5 at 72 °C, and one termination cycle of at 72 °C, with 35 cycles in total for HSF1a and HSF1b and 30 for heat shock cognate 70 (HSC70) Rainbow ... including introns and an ORF encoding 513 amino acids Ó FEBS 2004 By using 5¢- and 3¢-RACE, the full-length cDNAs of clones C1 and C2 without introns were determined to be 2083 bp and 2142 bp, respectively ... trout HSF1a and HSF1b (78.8% identity; Fig 3B) Ó FEBS 2004 708 N Ojima and M Yamashita (Eur J Biochem 271) Fig RT-PCR analysis of the HSF1a and HSF1b genes in rainbow trout RTG-2 cells and tissues...
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