insights about substrate recognition acquisition and release
Insights into solid phase characteristics and release of heavy metals and arsenic from industrial sludge via combined chemical, mineralogical, and microanalysis
... to SO42− and PO43−, the samples show the following order for released amount: SI2> SE>SI1 for SO42− and SI1>SI2>SE for PO43− pHstat leaching test Heavy metals and As Figures and display release ... of Cr and Ni (13,208 and 55,732 mg/kg, respectively) (Table 2) Chemical data related to samples SI1, SI2, and SE and a comparison with published data from electroplating sludges (Sophia and Swaminathan ... reveal the presence of quartz (spots and 2, Table and Fig 2), phosphorus (spots 3, 4, 5, 6, 7, 12, and 13), and sulfur species Although some HMs (Cr, Ni, and Zn) were detected with FEG-EPMA,...
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Tài liệu Báo cáo khoa học: Structure of RNase Sa2 complexes with mononucleotides – new aspects of catalytic reaction and substrate recognition pptx
... molecules C and D of 3DH2, the guanine base is shifted ˚ by 1.9 A towards Glu43 and Arg42, and the phosphate ˚ group by about 1.4 A However, the weaker electron density for the mononucleotide and surrounding ... which is turned by 90° and sandwiched between His86 and Thr69 (Fig 4C) The second phosphate group of the trinucleotide is positioned between Asp66–Thr69 and Arg34 NH1 and ˚ NH2, which are 3.2 ... Sa2 and RNase Sa, and to better understand the function of the amino acids involved in catalysis, we analyzed the active sites of RNase Sa2, RNase Sa (2SAR, 1RSN, and 1GMP), binase (1GOY) and...
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Tài liệu Báo cáo khoa học: Structural insights into the substrate specificity and activity of ervatamins, the papain-like cysteine proteases from a tropical plant, Ervatamia coronaria ppt
... catalytic mechanism and substrate specificity and to understand the differences therein for this class of enzymes In order to identify the subsites of the ervatamins and to understand substrate or inhibitor ... stability, substrate specificity, and activity The stability aspect has been extensively discussed in our earlier papers [27,35], and here we address the issue of substrate specificity and activity ... [27,35] and other members of the papain family, and is made up of two domains, L and R, with a V-shaped active site cleft at the interface of the domains (Fig 1) Substrate specificity and activity...
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Báo cáo khoa học: Conserved residues in the N-domain of the AAA+ chaperone ClpA regulate substrate recognition and unfolding pdf
... Leu109 and Val134) located in close proximity to R90 and R131 and a substrate (data not shown) Importantly, these variants were able to crosslink to ClpS and mediated the degradation of GFP–ssrA and ... efficiently Substrate recognition and unfolding by ClpA Results Two conserved arginine residues (R90 and R131) within the N-domain are required for full ClpA function We were interested to understand ... delayed release of substrate, which results in reduced unfolding of the substrate Discussion As for most AAA+ proteases, ClpA utilizes the hydrolysis of ATP to drive substrate unfolding and translocation...
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Báo cáo khoa học: Insights into substrate and product traffic in the Drosophila melanogaster acetylcholinesterase active site gorge by enlarging a back channel ppt
... inhibition of substrate hydrolysis and acceleration of decarbamoylation by substrate analogue [2], inhibition of substrate hydrolysis by reversible inhibitors [13–17], hydrolysis of substrate at ... hydrolysis of substrate at different temperatures [18] and hydrolysis of different substrates [19] For the purposes of alternative substrate traffic in and out of the active site of DmAChE, the mutation ... enzyme activity at different substrate concentrations) for acetylthiocholine hydrolysis by Glu and Ala mutants, however, were shifted to higher concentrations of substrate and became symmetric Consequently,...
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Báo cáo khoa học: The twin-arginine translocation (Tat) systems from Bacillus subtilis display a conserved mode of complex organization and similar substrate recognition requirements doc
... both the TatAdCd and TatAyCy pathways are active in E coli and able to recognize several different E coli Tat signal peptides, but not all substrates are compatible and a degree of substrate specificity ... with NheI and XbaI and ligated into NheI ⁄ XbaI-cut pEXT22 to construct pEXT–AyCy For construction of pBAD–AmiA–GFP and MdoD– GFP the signal sequences for the two Tat substrates AmiA and MdoD ... active in E coli and able to export fusion proteins comprising the signal peptides of TorA and DmsA linked to GFP [8] Separate TatAdCd and TatAd complexes were characterized and shown to be very...
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Báo cáo khoa học: Structural flexibility of the methanogenic-type seryl-tRNA synthetase active site and its implication for specific substrate recognition pptx
... binding of ATP and the amino acid substrate in the active site are linked, and incorrect binding of ATP can affect serine binding and vice versa [22] Asn435, together with Asp416 and Glu432, contributes ... protein ligands (Cys306, Glu355 and Cys461; marked with asterisks in Fig 1A) and a water molecule, which dissociates from the zinc ion to allow coordination of the amino group of the serine substrate ... structure–functional and evolutionary terms Using mutational and kinetic analysis, we evaluated the importance of mMbSerRS active site flexibility in the discriminating between substrates and the proper...
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Báo cáo khoa học: Substrate recognition and ®delity of strand joining by an archaeal DNA ligase docx
... ATP, and 50 nM LigTk DNA substrate [L-DNA(40), R-DNA(30), and T-DNA(80)] were added at various concentrations in the range 0.5±4 lM With radiolabelled DNA substrates, 0.1 lM L-DNA, 0.1 lM T-DNA and ... concentration of 20 lM, the substrates, L-DNA(40) and R-DNA(30), were consumed almost completely and ligated by 0.2 lM of LigTk The concentrations of L-DNA(40) and R-DNA(30) were lM each and considerably ... R-DNA(30) and T-DNA(80) The reactions were performed with labelled oligonucleotides as described in Materials and methods (A) Ligation of 3¢ matched and 3¢ mismatched substrates The substrates...
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Báo cáo khoa học: Structure of a trypanosomatid mitochondrial cytochrome c with heme attached via only one thioether bond and implications for the substrate recognition requirements of heme lyase potx
... organisms and organelles Some eukaryotes (land plants and various protists) use the multicomponent System I [also called the cytochrome c maturation (Ccm) system], and others (animals, fungi, and many ... diverse protozoa and algae) use System III, the enzyme heme lyase, for maturation of their mitochondrial cytochromes c [10] Surprisingly little is known about the mechanism and substrate recognition ... in ball and stick representation, as are the methionine and histidine side chains that coordinate the heme iron, and the cysteine side chain that forms a thioether bond between heme and protein...
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Báo cáo khoa học: Substrate recognition by three family 13 yeast a-glucosidases Evaluation of deoxygenated and conformationally biased isomaltosides pptx
... Mutational analysis of the roles in catalysis and substrate recognition of arginines 54 and 305, aspartic acid 309, and tryptophan 317 located at subsites and in glucoamylase from Aspergillus niger ... activities and substrate af®nities are similar for these retaining and inverting enzymes, all of which have reasonable capacity in the glucose release from the nonreducing end of disaccharides and small ... different substrate concentrations from 0.1 ´ Km to ´ Km to the Michealis±Menten equation essentially as described previously [40] MATERIALS AND METHODS Reaction stereochemistry Enzymes and substrates...
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Báo cáo khoa học: Molecular basis for substrate recognition and drug ˚ resistance from 1.1 to 1.6 A resolution crystal structures of HIV-1 protease mutants with substrate analogs pptx
... residues with longer and flexible side chains, such as Lys The number of alternate conformations was in the Comparison of ligand bound and unliganded PR structures [26,27] and theoretical studies ... p2-NC Ile 84 structures and mediate the interactions between the PR and the inhibitor One conserved water molecules lies between the flap region (Ile50 and 50¢) and P2 and P1¢ of the inhibitor, ... understanding the specificity of substrate recognition and a basic framework for the design of new inhibitors that are more effective against resistant HIV Experimental procedures Expression and...
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Báo cáo lâm nghiệp: "New insights in the recognition of the European ash species Fraxinus excelsior L. and Fraxinus angustifolia Vahl as useful tools for forest management" pdf
... R.G., Understanding and confronting species uncertainty in biology and conservation, Trends Ecol Evol 18 (2003) 597–603 [6] Jeandroz S., Frascaria-Lacoste N., Bousquet J., Molecular recognition ... Masimbert and two anonymous reviewers for helpful comments on the manuscript and discussions, Francina Langa and Jérôme Artus (Pasteur Institute) for the use of their thermocycler, and Lionel ... primers OpL03 and OpH04 (Qiagen/Operon) Three F angustifolia-specific bands were excised from a 1.5% agarose gel after Ethidium Bromide staining: OpH04 1600 bp and OpL03 750 bp described in Jeandroz...
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101 ielts speaking part two tasks about the past present and future
... How long you stayed there - What the good and bad things about it were And say whether you would like to stay in the same place again, and why Talk about one method of learning a foreign language ... it And say if you think it is well designed and well made or not, and why Talk about a job someone you know does You should say: - How long they have had that job - What things they like about ... say: - What the local landmarks are - What green spaces and natural features there are - What things there are to And say what you would change about the area if you could, and why Describe a house...
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Effect of Coagulant on Phosphorus Uptake and Release in EBPR Process
... mg/L and SRT was about 30 days in each reactor Effluent SS and D-BOD were less than 20 mg/L and mg/L, respectively Effluent T-N mainly consisted of NO3-N was 13.5 mg/L and 9.50 mg/L, in SBR1 and ... phosphorus uptake and release was discussed by comparing these indices Fig shows the average and standard deviation of vrel, Cmax and kupt which values were calculated from 7, 9, and data in Run1-1, ... acetate and glucose (Cech and Hartman, 1990) Acetate and glucose were fed to the activated sludge in this experiment, therefore it was thought that both PAOs and GAOs were cultured in the SBR tank and...
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Tài liệu Cognitive Impairment in the Elderly – Recognition, Diagnosis and Management docx
... understand their present condition and prognosis, and have they been able to participate in the development of goals and realistic expectations for treatment? • Is the patient a suitable candidate ... system and burden • Educate patients and caregivers about the disease and how to cope, including advance care planning (consider cultural context for understanding and acceptance of dementia; see ... left handed Take a piece of paper, hold it up in front of the person and say: Take this paper in your right/left hand (whichever is non-dominant), fold the paper in half once with both hands and...
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Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf
... of seven a-helices, a three-stranded antiparallel b-sheet and loops connecting these secondary structure elements The contents of the a-helix and b-strand were 50% and 11%, respectively The monomer ... (B) and (C) Prepared with PYMOL [44] C of the N-terminal extended region and the following two a-helices, and the globular, (a + b)-type C-terminal domain Oligomeric structure The A ⁄ B and C ... coordinated with six ligands: the five same ligands in the Mn-reconstituted enzyme and an additional water oxygen, which, together with the OD2 of Asp165, the NE2 of His79 and the NE2 of His169,...
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Tài liệu Báo cáo khoa học: Degradation of tropoelastin by matrix metalloproteinases – cleavage site specificities and release of matrikines pptx
... for binding to EBP and that the released peptides display bioactivity [19] The sequence GPQPG, which is released in longer peptides by MMP-7 and MMP-12, seems to be a good candidate for interaction ... YGARPGVGVGGIP, and PGFGAVPGA, that were released during digestion of tropoelastin by MMP-7, MMP-9, and ⁄ or MMP-12 (Fig 1; Tables and 4) The three peptides contain the GxxPG motifs GYGPG, GARPG, and GAVPG, ... low-mass and high-mass resolution settings of 10 (arbitrary units), and the collison energy was varied between 25 eV and 60 eV depending on charge state and m/z value Data processing and peptide...
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Tài liệu Misconceptions About Environmental Pollution, Pesticides and the Causes of Cancer doc
... include Vitamins C and E and perhaps carotenoids,13 most of which come from dietary fruits and vegetables “Smoking contributes to about 35 percent of cancer.” Smoking contributes to about 35 percent ... West Sussex, England) 29 Krebs-Smith, S.M., Cook, A., Subar, A F., Cleveland, L., Friday, J., and Kahle, L L (1996) Archives of Pediatrics and Adolescent Medicine 150, 81-6; and Krebs-Smith, ... been tested and 19 are rodent carcinogens Plants in the human diet contain thousands of natural pesticides that protect them from insects and other predators: 63 have been tested and 35 are rodent...
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Tài liệu Báo cáo khoa học: Solution structure of hirsutellin A – new insights into the active site and interacting interfaces of ribotoxins docx
... acid and a base on each side of the hydrolyzed bond [16] E96 and H137 in a-sarcin and E58 and H92 in RNase T1 act as the base and acid groups, respectively Comparing the 3D structures, E66 and ... N56–D58) and seven b-strands (b1, I3– C6; b2, F17–D20; b3, H42–Y44; b4, L64–P68; b5, R95– 2384 A99; b6, G109–H113; b7: F126–K128) The b-strands form an N-terminal hairpin (b1 and b2) and an antiparallel ... A Fig 1Ha and 1HN conformational shifts (dobserved–drandom coil) in ppm for HtA at pH 4.1 and 298 K The amino acid sequence and the elements of secondary structure are shown; b-strands are represented...
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Tài liệu Báo cáo khoa học: Substrate specificity and inhibition of brassinin hydrolases, detoxifying enzymes from the plant pathogens Leptosphaeria maculans and Alternaria brassicicola ppt
... 2-mercaptoethanol and dithiothreitol For example, mm dithiothreitol caused 72% and 85% inhibition of BHLmL2 and BHAb, respectively, while mm 2-mer- captoethanol caused 85% and 97% inhibition of BHLmL2 and ... phytoalexin brassinin (1, natural substrate) and synthetic substrates 1-methylbrassinin (4), methyl tryptaminedithiocarbamate (8) and methyl tryptopholdithiocarbonate (16) Substrate ⁄ compound name (number) ... Chemical structures of compounds tested as substrates of BHLmL2 and BHAb and potential products, references for synthesis, and HPLC retention times using methods A and B described in the Experimental...
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