inside outside both of model but must be within projection range
Báo cáo y học: " Functions, structure, and read-through alternative splicing of feline APOBEC3 genes" ppt
Ngày tải lên :
14/08/2014, 08:20
... andandbindingCT,nucleotideofofAPOBEC3H (b)in coding APOBEC3A DNAwindow,S3:Match,againstdescribed mutation and APOBEC3C, APOBEC3FAPOBEC3G Ka/Ks(A3Ca,(b) felinedifferAPOBEC3 acid in eight domestic ... Therapsida Figure Model of mammalian A3 gene evolution Model of mammalian A3 gene evolution The model proposes the presence of several A3 genes in Placentalia before the separation of the super-orders ... genomic APOBEC3 region of the domestic cat (F catus) and found a chromosomal APOBEC3 locus different to that of primates, rodents and dogs Besides our detection of three very similar APOBEC3C genes...
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Báo cáo khoa học: Purification, characterization, cDNA cloning and nucleotide sequencing of a cellulase from the yellow-spotted longicorn beetle, Psacothea hilaris ppt
Ngày tải lên :
17/03/2014, 10:20
... cellulase, which belongs to GHF 45, has been cloned from a gut library from the phytophagous beetle, Phaedon cochleariae [24] This GHF 45 cellulase has been confirmed to be expressed in the gut of P cochleariae ... ORF consisted of a protein of 325 amino acids The molecular mass of P hilaris Fig Comparison of amino-acid sequences for potential catalytic proton donor and nucleophile regions of P hilaris cellulase ... cubes) and transferred to 1.5 mL centrifugal micro tubes The micro tubes were filled with distilled water and kept at °C overnight to allow elution of the proteins from the gel sections The tubes...
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Báo cáo khoa học: "Isolation and identification of Escherichia coli O157:H7 using different detection methods and molecular determination by multiplex PCR and RAPD" docx
Ngày tải lên :
07/08/2014, 18:21
... has been often associated with the consumption of contaminated ground beef, raw milk, and other bovine products, thus cattle are suspected to be a primary reservoir [15] But bacteria also have been ... population will be assigned to different typing groups The formula of D-value is as follows S D = – ⁄ N ( N – ) ∑ nj ( nj – ) j=1 S = total number of different types, nj = number of isolates representing ... (7/1,682) 0.59 (10/1,682) 0.65 (11/1,682) a No of positive/No of samples examined No of negative/No of samples examined b Table Antibiotic resistance profiles of isolated E coli O157:H7 Antimicrobial...
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báo cáo khoa học: " Isolation, identification and expression analysis of salt-induced genes in Suaeda maritima, a natural halophyte, using PCR-based suppression subtractive hybridization" doc
Ngày tải lên :
12/08/2014, 03:20
... glycinebetaine, the enzyme appears to be very important in the biochemical pathways of synthesis of the osmoticum The activity of PEAMT has been reported earlier to be greatly enhanced in the betaine ... could be because the availability of choline is probably more important for the accumulation of glycinebetaine than the amount of the enzymes catalysing the conversion of choline to glycinebetaine, ... to be very important as the size of the ESTs of this category (Fig 7) was more than 10 fold the size of the unigenes (Fig 6) in the group Besides, the ESTs of the pro- Page 21 of 25 (page number...
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báo cáo khoa học: " An optimized grapevine RNA isolation procedure and statistical determination of reference genes for real-time RT-PCR during berry development" docx
Ngày tải lên :
12/08/2014, 05:20
... geometric mean of a greater number of genes for normalization RNA isolation Young grape berries have a pH between 2.0 and 3.0 (Figure 4), while ripe berries contain high levels of polysaccharide ... 14 13 12 11 10 Number of Remaining Reference Genes Figure development series geNorm2ranking of reference genes over a grape berry geNorm ranking of reference genes over a grape berry development ... β-tubulin, PP2A, and SAND, with Ct values of 26 or higher The calculated coefficient of variance of the Ct values gives an indication of the expression stability of a particular gene β-tubulin and cyclophilin...
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Báo cáo khoa học: Anti-HIV-1 activity of 3-deaza-adenosine analogs Inhibition of S-adenosylhomocysteine hydrolase and nucleotide congeners pot
Ngày tải lên :
08/03/2014, 08:20
... effects of the DZA analogs have been attributed to their potent inhibition of AdoHcy hydrolase and the attendant inhibition of methylation reactions [1,7,15,16] However, the antiviral mechanism of ... contribution of these two mechanisms: (a) indirect inhibition of methylation via the direct inhibition of AdoHcy hydrolase, and (b) intracellular phosphorylation of the DZA analogs to become ... expressed herein are those of the authors and not reflect the of cial position of the US Army or the Department of Defense Guidelines for human experimentation of the US Department of Defense were followed...
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Báo cáo khoa học: Isolation, characterization and expression analysis of a hypoxia-responsive glucose transporter gene from the grass carp, Ctenopharyngodon idellus potx
Ngày tải lên :
17/03/2014, 03:20
... analysis showed that both GT-cDNA1 and GT-cDNA2 contain a 5¢-UTR of 203 bp and a coding region of 1599 bp (excluding the stop codon), but the 3¢-UTR of GT-cDNA1 is 345 bp while that of GTcDNA2 is 1238 ... of coho salmon, and 50% with GLUT2 of rainbow trout [11] No report of GLUT3 has yet been described in fish Although we were unable to predict the actual isoform of gcGLUT based on sequence identity ... Characteristics of the deduced amino acid sequence of gcGLUT Analysis of the deduced amino acid sequence of GTcDNA1 with the HMMTOP program (http://www.enzim.hu/ hmmtop/) [17] indicated the presence of 12...
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Báo cáo " Isomeranzin against Herpes simplex virus in vitro from Clausena heptaphylla (Roxb.) W. & ARN.: Isolation, structure and biological assay " potx
Ngày tải lên :
03/04/2014, 15:20
... role of solvent at lower concentrations (2.5% ethyl acetate plus virus), examining the effectiveness of a water extract of the plant, or elucidating the mechanism of synergistic cytotoxicity of ... Vietnam Methods of biological activity assay [6 - 9] The sample was prepared in four solvents It was prepared at a concentration of mg/ml in 0.85% NaCl plus a 10% solution of one of the three solvents: ... spectrum of the product (500 MHz, CDCl3) Figure 4: 13C-NMR spectrum of the product (500 MHz, CDCl3) 117 The 13C-NMR spectrum also gave support to this assignment Thus, 15 carbon atoms of obtained...
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fattorusso - modern alkaloids - structure, isolation, synthesis and biology (wiley, 2008)
Ngày tải lên :
04/06/2014, 15:25
... one of the widest classes of natural products, being synthesized practically by all phyla of both marine and terrestrial organisms, at any evolutionary level The extraordinary variety (and often ... Mississippi, MS 38677 USA List of Contributors Karumanchi V Rao University of Mississippi Department of Pharmacognosy Mississippi, MS 38677 USA Michel Roberge University of British Columbia 2146 Health ... plant, be it a herbivore or a bacterium, fungus, or virus These classes of compounds are seldom unique constituents; quite often plants produce a mixture of secondary metabolites, often both phenolics...
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báo cáo hóa học:" Validation of a HLA-A2 tetramer flow cytometric method, IFNgamma real time RT-PCR, and IFNgamma ELISPOT for detection of immunologic response to gp100 and MelanA/MART-1 in melanoma patients" doc
Ngày tải lên :
18/06/2014, 15:20
... ELISPOT, assessment of assay sensitivity depends on number of TIL cells spiked into the number of PBMCs as the negative cell population Due to the limited number of PBMC that could be obtained from ... Precision The precision of an analytical method describes the closeness of agreement (degree of scatter) between a series of measurements obtained from multiple sampling of the same homogenous ... and from the results of the normal distribution study (Table 8) Data from the normal distribution study showed the number of background IFNγ secreting cells (Mean + SD) to be as follows: gp100209...
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Báo cáo sinh học: " Development of a real-time RT-PCR and Reverse Line probe Hybridisation assay for the routine detection and genotyping of Noroviruses in Ireland" pdf
Ngày tải lên :
18/06/2014, 18:20
... per gram of stool was 1.02 × 109molecules Design of oligonucleotide probes for development of reverse line probe hybridization assay Twenty-five oligonucleotide probes were designed within the ... membrane Top of the figure indicates where denatured PCR products of stool panel have been applied Presence of spot indicates probe binding Gaps between spots indicate unbound probes for which ... respective probes Clinical samples binding to probe GII/4 can be seen on the right of both A and B Biotinylated RT-PCR Biotinylated reverse primers G1NVR and G2NVR synthesized by MWG Biotech (Ebersberg,...
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Báo cáo sinh học: " Comparison of real-time PCR and hemagglutination assay for quantitation of human polyomavirus JC" pptx
Ngày tải lên :
19/06/2014, 08:20
... expressed as copies of viral DNA per 100 µL of virus suspension The reliability of real-time PCR was defined by calculating coefficients of variation of Ct values of replicates of standard curve ... using serial dilutions of the linearized JCV(Mad1) plasmid The dynamic range of detection was determined by preparing 10-fold serial dilutions of JCV plasmid in the range of 10 pg to fg, that represented ... volume of 20 µl of PCR mixture Copies of JCV(Mad1) T antigen gene were calculated from the standard curve and were expressed as copies of viral DNA per 100 µl of suspension A) Amplification plots of...
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Báo cáo hóa học: " Development of a real-time RT-PCR and Reverse Line probe Hybridisation assay for the routine detection and genotyping of Noroviruses in Ireland" ppt
Ngày tải lên :
20/06/2014, 01:20
... per gram of stool was 1.02 × 109molecules Design of oligonucleotide probes for development of reverse line probe hybridization assay Twenty-five oligonucleotide probes were designed within the ... membrane Top of the figure indicates where denatured PCR products of stool panel have been applied Presence of spot indicates probe binding Gaps between spots indicate unbound probes for which ... respective probes Clinical samples binding to probe GII/4 can be seen on the right of both A and B Biotinylated RT-PCR Biotinylated reverse primers G1NVR and G2NVR synthesized by MWG Biotech (Ebersberg,...
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báo cáo hóa học:"Comparison of real-time PCR and hemagglutination assay for quantitation of human polyomavirus JC" potx
Ngày tải lên :
20/06/2014, 04:20
... expressed as copies of viral DNA per 100 µL of virus suspension The reliability of real-time PCR was defined by calculating coefficients of variation of Ct values of replicates of standard curve ... using serial dilutions of the linearized JCV(Mad1) plasmid The dynamic range of detection was determined by preparing 10-fold serial dilutions of JCV plasmid in the range of 10 pg to fg, that represented ... volume of 20 µl of PCR mixture Copies of JCV(Mad1) T antigen gene were calculated from the standard curve and were expressed as copies of viral DNA per 100 µl of suspension A) Amplification plots of...
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Báo cáo nghiên cứu nông nghiệp " Isolation, inbreeding and relationships in forest trees " pdf
Ngày tải lên :
22/06/2014, 13:20
... Self-fertilised family inbreeding depression of yield pollen seed Forestry and Forest Products Inbreeding depression of growth in control-crossed progenies of E camaldulensis (Petford) at 30 months ... Rep Rep Row Rep tree row plots replicates each family occurs only once in replicate Within- plot thinning of seed orchards First assessment Replicate Replicate Plot Family Plot Family Related ... intensity Selection for development of seed orchards We selectively thin unpedigreed seed production areas, and pedigreed seed orchards, to achieve genetic gain Must thin to one tree per plot in...
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Báo cáo y học: "Contribution for new genetic markers of rheumatoid arthritis activity and severity: sequencing of the tumor necrosis factor-alpha gene promoter" docx
Ngày tải lên :
09/08/2014, 10:20
... intersect can be seen as cutoff points As can be seen in Figure 1, two cutoff points are expected to be found The cutoff points found are 85 and 115, meaning that SvdH score values below 85 are ... arthrodesis), the number of years of education, and work disability (defined as the legal incapacity to work as judged by an official medical committee and directly attributed to the consequences of RA) Patients ... more than 10 years of DD and those with less than 10 years of DD as a function of the SvdH score Results according to the duration of Sharp/van (DD) Estimated distribution of the the diseaseder...
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Rapid detection of epidermal growth factor receptor mutations with multiplex PCR and primer extension in lung cancer pdf
Ngày tải lên :
10/08/2014, 05:21
... analysis Various concentrations of probe for -216 promoter region and exons 18-21 were added to the tube containing 1.5 μl of purified PCR products (Table 2), as well as μl of ABI PRISM SNaPshot Multiplex ... labeled dideoxynucleotide triphosphates (ddNTPs) (RGG-labeled dideoxyadenosine triphosphate, TAMRAlabeled dideoxycytidine triphosphate, ROX-labeled dideoxythymidine triphosphate, and R110-labeled ... http://www.jbiomedsci.com/content/17/1/37 Page of Table 2: Primer extension mutation analysis probes for -216 promoter region and codons 719, 746-750, 790, and 858 of the EGFR genes Nucleotide number and sequence Amino...
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báo cáo khoa học: "Construction and EST sequencing of full-length, drought stress cDNA libraries for common beans (Phaseolus vulgaris L.)" doc
Ngày tải lên :
11/08/2014, 11:21
... average length of the contigs being 678 nt Meanwhile, the average length of the singletons was 568 nt The large number of singletons and the high number of contigs relative to the number of sequences ... and castor bean 18 Only 100 highest hits were directly with common bean genes These numbers of hits reflect both the similarity of the species, especially in the case of common bean-soybean, and ... sequencing of these clones substantially increases the number of unique EST sequences available for the common bean genome All of this should be useful for both functional gene annotation, analysis of...
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báo cáo khoa học: " Identification and characterization of wheat long non-protein coding RNAs responsive to powdery mildew infection and heat stress by using microarray analysis and SBS sequencing" ppsx
Ngày tải lên :
11/08/2014, 11:21
... reliable set of wheat long npcRNAs It must be pointed out that, in the absence of wheat whole genomic information and the full length sequences of these wheat long npcRNAs, some of them might ... transcript of the b-secretase-1 (BACE1) gene had been implicated in the progression of Alzheimer’s disease [35,36] Ben et al [18] found that abiotic stress altered the accumulation of 22 out of the ... form of long molecules involved in wheat resistance to powdery mildew infection and/or heat stressed The collection of long npcRNAs offered candidates for further analysis of this kind of npcRNAs,...
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Báo cáo khoa học: "Genomic variability in Potato virus M and the development of RT-PCR and RFLP procedures for the detection of this virus in seed potatoes" ppt
Ngày tải lên :
12/08/2014, 04:21
... and tubers) Up to 800 leaves or 400 sprouts could be combined for reliable detection of PVM RNA by RT-PCR Up to 200 to 400 dormant tubers could be combined to achieve reliable detection of PVM ... specificity None of them yielded any amplification products To evaluate the sensitivity of the RT-PCR for the detection of PVM in dormant tubers using primers PVM3/PVM4, sap from PVM infected tubers was ... same origin and both group IIa and IIb types of PVM isolates may be present in the same geographic region Based on the sequence alignment of known PVM strains/isolates, two sets of primers specific...
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