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induction and inhibition of drug metabolising enzymes

Tài liệu Báo cáo khoa học: Substrate specificity and inhibition of brassinin hydrolases, detoxifying enzymes from the plant pathogens Leptosphaeria maculans and Alternaria brassicicola ppt

Tài liệu Báo cáo khoa học: Substrate specificity and inhibition of brassinin hydrolases, detoxifying enzymes from the plant pathogens Leptosphaeria maculans and Alternaria brassicicola ppt

Báo cáo khoa học

... inhibition of BHLmL2 and BHAb, respectively, while mm 2-mer- captoethanol caused 85% and 97% inhibition of BHLmL2 and BHAb, respectively The influence of pH on the activities of the BH enzymes was ... specificities of brassinin hydrolase (BH) produced by L maculans L2 (BHLmL2) and BH produced by A brassicicola (BHAb) and report hereon the results of this work Results and Discussion Induction of BH ... concentrated and used for biochemical analysis SDS/PAGE of purified BHs Fig Effect of camalexin and 3-phenylindole on the activity of BH from mycelial cultures of Leptosphaeria maculans (A) and Alternaria...
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Báo cáo y học:

Báo cáo y học: " Induction of apoptosis and inhibition of cell growth by tbx5 knockdown contribute to dysmorphogenesis in Zebrafish embryos" pptx

Báo cáo khoa học

... activation of apoptosis and inhibition of cell growth in embryonic tbx5 morphants at 36 hpf by transcriptional and translational levels By RTPCR, the members of BCL-2 family genes and cell cycle-related ... molecules and eventually come to the end sequel of apoptosis and cease of cell growth Besides, the distribution of aberrant apoptosis and deviant cell cycle caused by a deficiency of tbx5 in ... wild-type and control mismatch (Figure 2B-D) By semi-quantitative PCR methods, the depletion of tbx5 caused an increase of the expression of S stagerelated mRNA: p27 and p57 (Figure 3A, B), and the...
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Báo cáo y học:

Báo cáo y học: "Risk and Benefit of Drug Use During Pregnancy"

Y học thưởng thức

... factors and when asked about the history of their pregnancy, give a long list of supposed agents On the other hand the mothers of healthy babies are thinking of the present and future of their ... hand we know that the critical period of some CAs exceeds the end of third month, e.g., the critical period of posterior cleft palate and hypospadias covers the 12th-14th and 14th-16th weeks of ... first occurrence and recurrence of neural-tube defects [52] Conflict of interest None declared References Jones KL, Smith DW, Ulleland CL et al Pattern of malformations in offspring of chronic alcoholic...
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Báo cáo khoa học: Cell biology, regulation and inhibition of b-secretase (BACE-1) potx

Báo cáo khoa học: Cell biology, regulation and inhibition of b-secretase (BACE-1) potx

Báo cáo khoa học

... full details) (B) Sites of cleavage of APP by b- and c-secretases to form Ab peptides The sites of the juxtamembrane and intramembrane cleavages of transmembrane APP by b- and c-secretases, respectively, ... Modulation of oligomeric state and hence activity of the enzyme Modulation of protein–protein interactions affecting localization and ⁄ or activity Modulation of lipid environment of the enzyme ... volunteers, and the drug was reported to be well tolerated and effective in lowering plasma Ab levels [122], and further trials are ongoing Future approaches and therapeutic potential of b-secretase inhibition...
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Báo cáo khoa học: MR solution structure of the precursor for carnobacteriocin B2, an antimicrobial peptide fromCarnobacterium piscicola Implications of the a-helical leader section for export and inhibition of type IIa bacteriocin activity pdf

Báo cáo khoa học: MR solution structure of the precursor for carnobacteriocin B2, an antimicrobial peptide fromCarnobacterium piscicola Implications of the a-helical leader section for export and inhibition of type IIa bacteriocin activity pdf

Báo cáo khoa học

... expression of a fusion of MBP and preCbnB2 in E coli This system allows use of (15NH4)2SO4, or alternatively (15NH4)2SO4 and 13 D-[U- C]glucose, as sole nitrogen and carbon sources Purification of substantial ... active peptide The goal of this study was determination of the 3D solution structure of preCbnB2 to assist in obtaining a molecular level understanding of the reduced activity of preCbnB2 (relative ... biochemical production, and solution structure of preCbnB2, and compare it with the structure of the mature bacteriocin, CbnB2 [26] [27] [28] Materials and methods Chemical synthesis of preCbnB2 with...
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Báo cáo khoa học: pH dependence, substrate specificity and inhibition of human kynurenine aminotransferase I ppt

Báo cáo khoa học: pH dependence, substrate specificity and inhibition of human kynurenine aminotransferase I ppt

Báo cáo khoa học

... grateful to Prof Bruce M Christensen, Department of Animal Health and Biomedical Sciences, University of Wisconsin (Madison, WI, USA) and Dr Menico Rizzi (Department of Genetics, University of Pavia, ... based on a standard curve generated using authentic KYNA and the specific activity of the enzyme was expressed as lmolÆmin)1Æmg)1 Effect of buffer and pH on hKAT-I To determine the effect of pH on hKAT-I ... protein standard (lane 3), respectively (B) Spectral changes of hKAT-I following the addition of 10 mM glutamine into hKAT-I solution The concomitant decrease of the 422 nm peak and increase of the...
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Cd toxicity effects on growth, mineral and chlorophyll contents and activities of stress related enzymes

Cd toxicity effects on growth, mineral and chlorophyll contents and activities of stress related enzymes

Môi trường

... accumulate and this results in the induction of enzymes such as POD, SOD and catalases (Creissen et al., 1994) These enzymes provide antioxidant protection and preserve membrane integrity The removal of ... suggested to be one of the main mechanisms of enzyme inhibition (Karataglis et al., 1991; Weigel and Jäger, 1980) Measured decreases in enzymes of intermediary metabolism (G6PDH, ICDH and ME) contrast ... with Zn A review of existing data on the induction of enzymes of the intermediary metabolism by several metals is given by Vangronsveld and Clijsters (1994) The fact that these enzymes seem to...
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Báo cáo khoa học:

Báo cáo khoa học: "Large-Scale Induction and Evaluation of Lexical Resources from the Penn-II Treebank" potx

Báo cáo khoa học

... system of (Briscoe and Carroll, 1997) distinguishes 163 verbal subcategorisation classes by means of a statistical shallow parser, a classifier of subcategorisation classes, and a priori estimates of ... annotation quality in terms of precision and recall against manually constructed, gold-standard f-structures for 105 randomly selected trees from section 23 of the WSJ section of Penn-II The algorithm ... higher than those of Schulte im Walde (op cit.) who recorded Precision of 60.76%, Recall of 63.91% and an F-score of 62.30% For Exp 3, we used semantic forms which contained details of specific prepositions...
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Báo cáo khoa học: Activation, regulation, and inhibition of DYRK1A Walter Becker1 and Wolfgang Sippl2 pptx

Báo cáo khoa học: Activation, regulation, and inhibition of DYRK1A Walter Becker1 and Wolfgang Sippl2 pptx

Báo cáo khoa học

... 2010 FEBS 251 Activation, regulation, and inhibition of DYRK1A W Becker and W Sippl Fig Structure of the ATP-binding pocket in DYRK1A and DYRK2 (A) Close-up of DYRK1A ATP binding pocket with docked ... physiological and pathological effects, this review focuses on the molecular mechanisms of activation and regulation of DYRK1A In addition, we provide an overview of the small molecule drugs that ... Effects of harmine on many other targets have been described, but generally require much higher concentrations than the inhibition of DYRK1A The antidiabetic effect of harmine as an inducer of Id2 and...
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Báo cáo khoa học: Dynamics of a-synuclein aggregation and inhibition of pore-like oligomer development by b-synuclein doc

Báo cáo khoa học: Dynamics of a-synuclein aggregation and inhibition of pore-like oligomer development by b-synuclein doc

Báo cáo khoa học

... modeled b- and b-syn, and b- and a-syn heterodimeric interactions Firstly, theoretical docking of various molecular dynamics conformers of a-syn to conformers of b-syn was performed All of the docked ... that in the absence of metals, b-syn interacts with a-syn and is capable of preventing a-syn aggregation and related deficits both in vitro and in vivo [23,24] Various lines of evidence support ... has analyzed by NMR the micelle-bound structure and dynamics of b- and c-syn [41] Thus, better understanding of the steps involved in the process of a-syn aggregation is important in order to develop...
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Báo cáo khoa học: Adipophilin increases triglyceride storage in human macrophages by stimulation of biosynthesis and inhibition of b-oxidation doc

Báo cáo khoa học: Adipophilin increases triglyceride storage in human macrophages by stimulation of biosynthesis and inhibition of b-oxidation doc

Báo cáo khoa học

... multiplicity of infection values (m.o.i.) of 100 and 500, adipophilin mRNA was increased  14 ± 0.8- and  39 ± 7.8-fold, respectively, and adipophilin protein was increased  6.5 ± 1.7- and  38 ... Ad.CMV.adipophilin-infected macrophages loaded or not with 10 and 100 lgÆmL)1 of AcLDL (data not shown) The induction of adipophilin expression and the excessive lipid loading of THP-1 macrophages in response to VLDL ... plasmids and pAdEASY-1 [36] and plaque purified High titre stocks of Ad.CMV.adipophilin and Ad.CMV.GFP (2.7 · 1012 and 8.5 · 1012 viral particlesÆmL)1, respectively) were produced in 293 cells and...
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Báo cáo y học:

Báo cáo y học: "Strong inhibition of TNF-α production and inhibition of IL-8 and COX-2 mRNA expression in monocyte-derived macrophages by RWJ 67657, a p38 mitogen-activated protein kinase (MAPK) inhibitor" ppt

Báo cáo khoa học

... Plesner and co-workers [18], and compared MDMs from healthy controls and RA patients Strong inhibition of mRNA expression and production of TNF-α by RWJ 67657 was found, as well as inhibition of IL-1β, ... fibroblasts and macrophages, both important players in the process of inflammation by the production of cytokines and degrading enzymes Recently our group demonstrated strong inhibition of IL-6, ... µM and the complete inhibition of MAPKAPK2 phosphorylation at 0.1 µM RWJ 67657 The solvent, 0.1% DMSO, did not affect phosphorylation of either kinase Results Effect of RWJ 67657 on cytokine and...
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Báo cáo khoa học:

Báo cáo khoa học: "The conformation change of Bcl-2 is involved in arsenic trioxide-induced apoptosis and inhibition of proliferation in SGC7901 human gastric cancer cells" doc

Báo cáo khoa học

... concentration of the drug which is capable of bringing about 50% inhibition of cell survival) of the drug used for treatment was determined by plotting a graph with inhibitory rate of cell growth ... including inhibition of telomerase activity, induction of reactive oxygen species release, and inhibition of survival pathways involving extracellular signal regulated kinase, Akt, calcium signaling and ... phenotype and drug resistance to various categories of chemotherapeutic drugs in malignancies Small molecule inhibitors of the Bcl-2 family proteins, designed to bind the hydrophobic groove of Zheng...
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Báo cáo khoa học:

Báo cáo khoa học: "Ionizing radiation and inhibition of angiogenesis in a spontaneous mammary carcinoma and in a syngenic heterotopic allograft tumor model: a comparative study." pdf

Báo cáo khoa học

... day of treatment, mice were sacrificed, and tumors were harvested, formalin fixed, and stained for the Ki-67 (A), TUNEL (B), and CD31 (C) as marker of tumor cell proliferation, apoptosis, and ... cells and Ki-67- and TUNEL-positive nuclei per highpowered fields (hpf) was determined in 4-10 randomly chosen visual fields in each of at least two similarly treated vital tumor tissues of allografts ... experiments AB-T and VV participated in the in vivo studies MP conceived of the study, participated in its design and coordination and finalized the manuscript All authors read and approved the...
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Báo cáo y học:

Báo cáo y học: "Celastrus aculeatus Merr. suppresses the induction and progression of autoimmune arthritis by modulating immune response to heat-shock protein 65" pptx

Báo cáo khoa học

... severity of ongoing AA in the LEW rat and the level of this effect is comparable to that of MTX We have described above that feeding of Celastrus to naïve LEW rats beginning prior to the induction of ... 72 h of cell culture and tested in ELISA for (a) IFN-γ and (b) IL-10 The results are expressed as pg/ml (mean + standard error of the mean) The difference in the level of IL-10 but not of IFN-γ ... hind paw was examined and the results are presented as median (interquartile range) Preparation and characterization of the ethanol extract of Celastrus The roots and stems of Celastrus aculeatus...
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Báo cáo y học:

Báo cáo y học: " Effectiveness of antiretroviral therapy and development of drug resistance in HIV-1 infected patients in Mombasa, Kenya" doc

Báo cáo khoa học

... treatment failure and accompanying resistance are of concern with regard to the risk of disease progression and potential transmission of drug resistant virus to partners The aim of this study was ... had a viral load of 533 and 1,380 copies/ml after 23 and 26 months of treatment respectively Discussion Intensive campaigns to improve availability of ART worldwide is paying off and most African ... Accumulation of mutations against drugs from different drug classes and/ or the presence of broad cross-resistance mutations will jeopardize the effectiveness of the NRTI backbone of second-line...
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báo cáo khoa học:

báo cáo khoa học: " The evolutionary origins and significance of drug addiction" potx

Báo cáo khoa học

... target of a wide range of drugs, including marijuana and cocaine, increasing the transmission of dopamine to the nucleus accumbens [10] This system mediates emotion and controls reinforcement, and ... addiction and the evolution of emotion Drugs distribution and use in ancient environments When examining the distribution of natural drugs in ancestral environment we see that there was often a ... The nature of drug addiction is three-fold: biological, psychological, and social Although humans may be biologically and psychologically predisposed to drug use and addiction, they may often be...
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Báo cáo y học:

Báo cáo y học: " Post-transcriptional control by bacteriophage T4: mRNA decay and inhibition of translation initiation" pdf

Báo cáo khoa học

... inactivation of PNK increases the stability of some RegB-processed transcripts (Durand et al 2008, unpublished data) Thus, both the kinase and phosphatase activities of PNK control the degradation of some ... inhibition of phage development Protein synthesis is normal until the beginning of the middle period and collapses thereafter A number of endonucleolytic cleavages can be detected in middle and ... in infection of an RNase E host mutant (rneΔ131) unable to assemble the degradosome [48] The specificity of RNase LS and coupling with translation The specificity and mode of action of RNase LS...
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Báo cáo y học:

Báo cáo y học: " Induction and regulation of matrix metalloproteinase-12in human airway smooth muscle cells" ppt

Báo cáo khoa học

... expression IL-1β-stimulated MMP-12 Inhibition ofby PD98059 and SP600125 activity and mRNA Inhibition of IL-1β-stimulated MMP-12 activity and mRNA expression by PD98059 and SP600125 ASMC were pretreated ... asthma and emphysema/COPD [24-26], and induce the induction of a number of inflammatory mediators by ASMC [27,28] Increased expression of IL-1β has been detected in airway epithelial cells and alveolar ... mRNA expression and c-Jun activation activity, Effects of TNF-α and TGF-β1 on IL-1β-stimulated MMP-12 Effects of TNF-α and TGF-β1 on IL-1β-stimulated MMP-12 activity, mRNA expression and c-Jun activation...
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Báo cáo y học:

Báo cáo y học: " APOBEC3G-UBA2 fusion as a potential strategy for stable expression of APOBEC3G and inhibition of HIV-1 replication" pot

Báo cáo khoa học

... anti-HA, and anti-β-actin antibodies respectively A-b The intensity of APOBEC3G protein and value of the relative intensity of APOBEC3G was determined as described in Fig Note, a protein band that ... total number of blue cells with the total number of cells counted Data shown represent average of three independent experiments Error Bars shown are standard errors of the means a results of wild ... contributions LL and DL carried out all of the experiments JYL provided supervision of the study and co-mentored LL's Ph.D dissertation RYZ supervised and directed the designed studies and co-mentored...
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