induced apoptosis in human keratinocyte stem cells
Báo cáo Y học: Requirement of caspase and p38MAPK activation in zinc-induced apoptosis in human leukemia HL-60 cells potx
... for Zn -induced apoptosis in human Burkitt lymphoma B cells An increase in labile Zn in the cytoplasm may suppress apoptosis by inhibition of the action of caspase, cytoplasmic apoptosis inducer, ... protein)1, indicating that the ability of Zn to induce apoptosis is dependent on the intracellular Zn level In fact, it has been shown that treatment with Zn (80–200 lM) induced apoptosis in mouse ... aspects in the regulation of apoptosis is the involvement of mitogen-activated protein kinases (MAPKs), a family of serine/threonine kinases that mediate intracellular signal transduction in response...
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báo cáo khoa học: "Basic Mechanisms of Arsenic Trioxide (ATO)Induced Apoptosis in Human Leukemia (HL-60) Cells" potx
... expression of annexin positive cells in ATO-treated cells compared to the control Annexin-V is a specific phosphatidylserine-binding protein used to detect apoptotic cells by providing an assessment ... seen in Figure 2, there was a gradual increase in annexin V positive cells (apoptotic cells) in ATO-treated cells compared to the control However, a marked and dose-dependent decrease in annexin ... granular contents into the surrounding extracellular space, with subsequent inflammation [30-32] To gain insight into the mechanism of arsenic trioxide (ATO) -induced apoptosis, we examined the modulation...
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Báo cáo y học: "Notochordal conditioned media from tissue increases proteoglycan accumulation and promotes a healthy nucleus pulposus phenotype in human mesenchymal stem cells" pot
... reverse (decoy) IPI human protein database containing the sequences of the proteins harboring the porcine-specific proteins identified in this study Using the same filtering criteria used for ... searched against the International Protein Index (IPI) non-redundant protein database using Sequest; requiring tryptic specificity; allowing precursor m/z tolerances of Da; allowing methionine residues ... species, including humans, during growth and aging, the NCs populating the NP disappear and are replaced by chondrocytic NP cells [33] The NP of some species retain notochord cells into maturity,...
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Role of sonic hedgehog signalling in human embryonic stem cells and its neural derivatives
... culture feeding 3.2.3 Human embryonic stem cells and induced pluripotent stem cells Human embryonic stem cell line HES-3 (46, XX) was from ES Cell International (Singapore, http://www.escellinternational.com) ... al., 2006) 2.7 Embryonic stem cells and induced pluripotent stem cells Embryonic stem cells (ESC) have two characteristics that distinguish them from other stem cells, in that they are capable ... forebrain, midbrain, hindbrain and the spinal cord This figure was reproduced from Epstein et al., 1999 2.4 SHH and neural development During the initial phase of neural induction, the ectoderm is induced...
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Báo cáo khoa học: " Cap-independent protein translation is initially responsible for 4-(N-methylnitrosamino)-1-(3-pyridyl)-butanone (NNK)-induced apoptosis in normal human bronchial pithelial cells" pot
... Our findings of rapamycininduced caspase activation is coincident with Nottingham et al’s [28] result that rapamycin expressed activated caspase in spinal cord of rats Rapamycin-dependent increase ... gamma-tocopherol quinone induced apoptosis in cancer cells through caspase activation and cytochrome c release [5] In FACS analysis, NNK induced significant apoptosis while live cells decreased in concentration-, ... protein level Rapamycin treatment induced significant increase in both Bax and Bid protein expression NNK treatment increased Bax protein expression in time-dependent manner, however, rapamycin...
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Báo cáo khoa học: " Synergistic effect of ERK inhibition on tetrandrine-induced apoptosis in A549 human lung carcinoma cells" docx
... of ERK inhibition on tetrandrine -induced apoptosis in A549 human lung carcinoma cells 25 Fig Flowcytometric detection of apoptosis of A549 cells treated with tetrandrine (TET) Cells were incubated ... examined TET treatment induced apoptosis and resulted in a decrease in Akt and ERK expression PI3K inhibition had no clear synergistic effect on tetradrine -induced apoptosis, however, ERK inhibition ... 10-14% in the USA [11] Therefore, it is clear that Synergistic effect of ERK inhibition on tetrandrine -induced apoptosis in A549 human lung carcinoma cells Fig The effects of a PI3K/Akt inhibitor...
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Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps
... defects in humans [5,6] However, to date, the delivery of MSCs into cartilaginous lesions has neither clinically nor experimentally resulted in sustained regeneration of hyaline cartilage in vivo ... resuspended in complete DMEM (containing 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin), and plated at to × 107 nucleated cells per 75 cm2 flask (Falcon, Beckton Dickinson Labware, Franklin ... or remained uninfected For comparison, an additional set of untransduced recombinant human protein controls were maintained, which were cultured in the presence of 10 ng/mL TGFβ1 protein, or...
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báo cáo khoa học: "Mitochondrial and endoplasmic reticulum stress pathways cooperate in zearalenone-induced apoptosis of human leukemic cells" doc
... volume and shown in this table as folds of increase or decrease (-) Fusarium [38,39] In the present study, ZEA induced apoptosis in human leukemic HL-60 and U937 cell lines, but less in PBMCs, as ... performed in in vivo model before using ZEA as a therapeutic drug Taken together, the intrinsic (mitochondrial) and ER stress pathways cooperated in ZEA -induced human leukemic cell apoptosis ... 13:363-373 16 Yamaguchi H, Wang HG: CHOP is involved in endoplasmic reticulum stress -induced apoptosis by enhancing DR5 expression in human carcinoma cells J Biol Chem 2004, 279:45495-45502 17...
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Báo cáo y học: "Proteomic analysis of mechanisms of hypoxia-induced apoptosis in trophoblastic cells"
... analyzed forty proteins by using the PowerBlot (BD Bioscience Pharmingen, San Diego, CA, USA) system We selected 40 apoptosis- related proteins to examine the mechanism of hypoxia -induced apoptosis After ... and methods Cell line and cell cultures The human choricarcinoma cell line JAR was obtained from American Type Culture Collection (Manassas, VA, USA) Cells were maintained in RPMI 1640 medium ... global changes in the proteins related to apoptosis during hypoxia to clarify the mechanism of hypoxia -induced apoptosis, and to find out useful hypoxia-related markers of trophoblastic cells Materials...
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Tài liệu Báo cáo Y học: Progressive apoptosis in chorion laeve trophoblast cells of human fetal membrane tissues during in vitro incubation is suppressed by antioxidative reagents pptx
... protects the cells from TNFa -induced U937 cell apoptosis by maintaining mitochondrial integrity and function [43], and ricin -induced apoptosis of U937 cells by maintaining an intracellular reducing condition ... Mn-SOD in human breast tumor MCF-7 cells suppressed apoptosis induced by ROS-generating agents [33], and that in cultured pheochromocytoma PC6 cells overexpression of Mn-SOD prevented apoptosis induced ... nitrogen intermediates, can induce apoptosis in various cells [25], if these oxidants can induce apoptosis, then antioxidants should be able to inhibit apoptosis Dithiocarbamates, including PDTC,...
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Báo cáo khoa học: Identification of carbonic anhydrase 9 as a contributor to pingyangmycin-induced drug resistance in human tongue cancer cells ppt
... GTP-binding protein rab22b Minichromosome maintenance protein homolog Ribosomal L1 domain containing Hepatocellular carcinoma-associated protein HCA10 Chromosome 20 ORF 30 Splicing factor proline ... protein), c11 Polyglutamine-binding protein FK506-binding protein Phosphoserine phosphatase-like Chromosome 15 ORF 24 Coiled-coil domain-containing protein 72 Dihydropyrimidinase-like ADP-ribosylation ... mechanism that prevents the apoptosis induced by cisplatin and doxorubicin [29,30] In addition, irinotecan -induced changes in MT expression correlated with clinical response in gastric cancer patients,...
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Báo cáo Y học: DAP kinase activity is critical for C2-ceramide-induced apoptosis in PC12 cells ppt
... kinase activity is critical for C2- and C8-ceramideinduced apoptosis in PC12 cells DISCUSSION DAP kinase is a Ca2+/calmodulin-regulated serine/threonine kinase that participates in apoptosis induced ... calmodulin-binding domain In the K42A-DK mutant, a lysine residue at position 42 in the kinase domain (asterisk) is replaced by an alanine The DD-DK construct contains the death domain and the serine-rich ... mediator of apoptosis [1±3] As Western blotting showed that DAP kinase was endogenously expressed in PC12 cells (Fig 2, lane 1), we examined whether the ceramide -induced apoptosis in PC12 cells was...
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Báo cáo khoa học: BRCA1 accumulates in the nucleus in response to hypoxia and TRAIL and enhances TRAIL-induced apoptosis in breast cancer cells pdf
... proteins involved in various cellular processes, including inhibition of apoptosis Hypoxia has been reported to inhibit apoptosis induced by tumor necrosis factor-related apoptosisinducing ligand ... hypoxia in MDA-MB-468 cells (Fig 2B), resulting in a 35% decrease in nuclear BRCA1 relative to the level in normoxic cells in the presence of 2E5 The decrease in C Fig Hypoxia -induced changes in BRCA1 ... the data in Fig support the involvement of a TRAIL-dependent process in mediating hypoxiainduced changes in BRCA1 localization TRAIL, but not hypoxia, induces apoptosis in breast cancer cells As...
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Báo cáo khoa học: Activation of p21-activated kinase 1 is required for lysophosphatidic acid-induced focal adhesion kinase phosphorylation and cell motility in human melanoma A2058 cells potx
... A2058 cells, cells were pretreated with either tyrosine kinase inhibitor, genistein, or MAP kinase inhibitor, PD98059 Inhibition of tyrosine kinase by genistein greatly inhibited LPA -induced PAK ... recruits other proteins, including paxillin, p130Cas, vinculin and talin in focal adhesions These protein complexes anchor the actin cytoskeleton and provide structural integrity to cells [16] The ... [20] The cells were maintained at 37 °C in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum in a humidified 5% CO2 For analysis of LPA -induced signals, cells in exponential...
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báo cáo hóa học:" Transplantation of vascular cells derived from human embryonic stem cells contributes to vascular regeneration after stroke in mice" docx
... human ES cells We induced differentiation of human ES cells in an in vitro two-dimensional culture on OP9 stromal cell line and examined the expression of VEGF-R2, VE-cadherin and TRA-1 during the ... Translational Medicine 2008, 6:54 cells, the number (cells/ mm2/mouse) of single stranded DNA (ss-DNA)+ cells in one field in the ischemic core from each mouse in the saline- or hES-ECs+MCs-injected group ... VEGF-R2-positive cells induced from undifferentiated mouse embryonic stem (ES) cells can differentiate into both VE-cadherin-positive endothelial cells (ECs) and αSMA-positive mural cells (MCs),...
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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
... [16,17] and mouse embryonic stem cells [18-20] The unique patterns of miRNA expression in embryonic stem cells suggest they are involved in maintaining "stemness" Identifying mRNAs that are directly ... upregulated in hES cells, node II contained the miRNAs upregulated in adult cells, node III contained the miRNAs upregulated in EB HMVEC = human microvascular endothelial cells, HUVEC = human umbilical ... groups: hES cells, EB, and adult cells Node A contained the genes that were upregulated in both hES cells and EB, node B contained the genes upregulated in hES cells only, node C contained the genes...
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báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx
... methylcytosine cells, chromatin of pluripotent cells appears to have unique features, such as the increased mobility of many structural chromatin proteins, including histones and heterochromatin protein ... signatures Cell Stem Cell 2009, 5:111-123 93 Chin MH, Pellegrini M, Plath K, Lowry WE: Molecular analyses of human induced pluripotent stem cells and embryonic stem cells Cell Stem Cell 2010, ... Rada-Iglesias A, Wysocka J: Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease Genome Medicine 2011, 3:36 ...
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Báo cáo y học: " Profiles of cytokine and chemokine gene expression in human pulmonary epithelial cells induced by human and avian influenza viruses" doc
... for growing stocks of influenza virus isolates MDCK cells were grown and maintained in Eagles Minimal Essential Media (MEM) containing 2% FBS, 100 U/ml penicillin and 100 μg/ml streptomycin (all ... prominent in H5N1/2004 infected cells, and the maximal up-regulation of these mRNA in H5N1/2004 infection occurred at 24 hours post-infection (Figure 2) Overall, the intensity of cytokine/chemokine ... Cytokine/chemokine protein profiles following infection To verify whether changes at the mRNA level were translated to protein level, the protein concentrations of cytokines/chemokines in cell...
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Báo cáo y học: " Highly pathogenic avian influenza A virus H5N1 NS1 protein induces caspase-dependent apoptosis in human alveolar basal epithelial cells" docx
... NS1 protein could induce apoptosis in human airway epithelial cells (NCI-H292) [26] It was also reported that a poor -apoptosis- inducer strain could be converted into a strong-inducer strain Zhang ... observations in the alveolar epithelial cells of two patients who died of H5N1 infection [13], have provided convincing evidence to the critical role of H5N1-encoded NS1 protein in inducing apoptosis Apoptosis, ... role in the execution-phase of cell apoptosis Recently, It has been reported that avian influenza virus A/HK/483/97(H5N1) NS1 protein -induced apoptosis in human lung epithelial cells is mainly...
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