increased glutaminolysis and expression of ido and arginase in the tumor environment

Báo cáo khóa học: Cloning and expression of murine enzymes involved in the salvage pathway of GDP-L-fucose ppt

Ngày tải lên : 30/03/2014, 13:20

... human fucokinase cDNA is similar to the long splice variant of mouse fucokinase cDNA at the 3¢ splice region The ﬁrst and third methionines in the murine sequence, in the upstream end of the CDS, ... neither is the splice junction between exon 23a or 23b, and exon 24 In conclusion, the alternative splicing maintains the reading frame along the entire protein, therefore the protein variants ... variants of the murine fucokinase genes were expressed in COS-7 cells in frame with a 10-amino acid E2Tag present in the pQM vector The molecular masses of fucokinase proteins were determined by...

9
437
0

Báo cáo khoa học: " Expression of tyrosine kinase A in the cerebral cortex of postnatal developing rat" docx

Ngày tải lên : 07/08/2014, 18:21

... rebmun ehT noitulid ,)ASU ,rotceV( seidobitna tibbar itna yradnoces detalynitoib htiw C ta thginrevo detabucni dna ,)spets gniwollof eht fo hcae neewteb demrofrep saw erudecorp hsaw emas( TR ta ... epotipe na sezingocer dna seidobitna lanolcylop deifirup ytiniffa si ydobitna yramirp sihT )ASU ,zurC atnaS( 001 : detulid ,nilubolgonummi AkrT-itna tibbar htiw C ta rh 84-42 rof detabucni erew ... rh 84 rof SBP ni esorcus %03 ni detcetorpoyrc neht dna C ta thginrevo evitaxif emas siht ni dexif-tsop erew seussiT desu erew ,2.7 Hp ,SBP M 1.0 ni edyhedlamrofarap %4 evitaxif yb dewollof ,)2.7Hp...

5
298
0

Báo cáo khoa học: "Increased expression of neuronal nitric oxide synthase in astrocytes and macrophages in the spinal cord of Lewis rats with autoimmune encephalomyelitis" pps

Ngày tải lên : 07/08/2014, 15:20

5
363
0

Báo cáo khoa học: Hierarchical subfunctionalization of fabp1a, fabp1b and fabp10 tissue-speciﬁc expression may account for retention of these duplicated genes in the zebraﬁsh (Danio rerio) genome docx

Ngày tải lên : 07/03/2014, 12:20

... FABP1 during zebrafish embryogenesis The distinct patterns of expression for the fabp1b (in the intestine) and fabp10 (in the liver) genes in the developing zebrafish combined represent the expression ... gradient in the levels of rat FABP1 mRNA is established in the intestine during the late fetal stage The mRNA concentration in the intestine increases sharply by three- to four-fold within 24 h of ... the timing of the insertion of intron in the fabp1a gene Linkage group assignment of zebrafish fabp1a, fabp1b and fabp10 genes using radiation hybrid mapping The fabp1a gene was assigned to linkage...

14
554
0

Báo cáo khoa học: Retention of the duplicated cellular retinoic acid-binding protein 1 genes (crabp1a and crabp1b) in the zebraﬁsh genome by subfunctionalization of tissue-speciﬁc expression doc

Ngày tải lên : 16/03/2014, 22:20

... of the brain, but not from any other tissues examined including the liver, ovary, skin, intestine, heart, muscle and testis crabp1b mRNA was detected by RT-PCR in the skin, intestine, brain and ... around the boarder of hindbrain and spinal cord showing transversal distribution of the crabp1b mRNA in r7, neurons of spinal cord (N Sp), dorsal and ventral spinal cord (D Sp and V Sp) and the ... 6B) in the optic tectum of the adult zebraﬁsh brain crabp1b mRNA was detected in the adult brain, muscle, intestine and skin but only by the sensitive technique of RT-PCR (Fig 6A) and not by in...

11
312
0

Báo cáo Y học: Expression of uncoupling protein-3 in subsarcolemmal and intermyoﬁbrillar mitochondria of various mouse muscle types and its modulation by fasting docx

Ngày tải lên : 24/03/2014, 04:21

... observed in the wild-type C2C12 cells whereas the human UCP3 was detected, at the expected size of 34 kDa, in the infected cells and in a sample of the human UCP3 recombinant protein Taken together these ... SEM from the number of experiments in parentheses SS Fig Increase in the UCP3 signal as a function of increasing amounts of (A) human recombinant protein and (B) mouse recombinant protein Representative ... Therefore the relative amounts of UCP3 protein and mRNA in the three muscles are comparable Effect of fasting on UCP3 protein expression We and others have shown that fasting induces upregulation of...

7
535
0

Báo cáo khoa học: Molecular identiﬁcation and expression study of differentially regulated genes in the Paciﬁc oyster Crassostrea gigas in response to pesticide exposure doc

Ngày tải lên : 30/03/2014, 15:20

... for and 21 days [22], and some of these genes were also present in the herbicide SSH libraries (including glutamine synthetase and cathepsin) In our experiments, we tested a cocktail of the three ... preventing the plant from producing an essential amino acid and by inhibiting the enzyme enolpyruvylshikimate-phosphate synthase which reduces the production of protein in the plant, thereby inhibiting ... [63] In the ﬁsh, Cyprinus carpio, an increase in GS was observed after several days of exposure to cypermethrin At the same time, there was a decrease in the amount of free amino acids coincident...

14
413
0

Báo cáo khoa học: Identiﬁcation, structure and differential expression of novel pleurocidins clustered on the genome of the winter ﬂounder, Pseudopleuronectes americanus (Walbaum) ppt

Ngày tải lên : 31/03/2014, 07:20

... predict the locations of the ﬁrst introns and exons of Y1, Y2 and Y3 An alignment of the peptides predicted from the pleurocidin genes and pseudogenes is shown in Fig All of the genes, and even ... WF2-like pleurocidin transcripts in mucus-producing cells of skin and goblet cells of small intestine in winter ﬂounder [21] In addition, Paneth cells within the human intestine have been shown ... possessing NFjB binding sites in their upstream sequences may exist elsewhere in the genome Octamer motifs (ATGCAAAT) are present in the noncoding sequences of all of the pleurocidins These motifs...

11
415
0

báo cáo hóa học: " Temporal expression and cellular origin of CC chemokine receptors CCR1, CCR2 and CCR5 in the central nervous system: insight into mechanisms of MOG-induced EAE" pptx

Ngày tải lên : 19/06/2014, 22:20

... areas of the spinal cord displaying focal inflammation and demyelination (Fig 2E) Quantification of CCR1, CCR2 and CCR5 mRNAexpressing cells in relation to the stage of demyelinating activity The ... immunization) of EAE show extensive inflammation involving the white and grey matter (D), with marked demyelination in the inflammatory areas (F) The majority of the infiltrating inflammatory ... apoptosis of infiltrating cells in the CNS of animals with EAE [80] The limitation of inflammation seen in the CNS could also be the result of a diminished antigen-presenting capability In conclusion,...

13
559
0

Báo cáo khoa học: "Altered expression of thioredoxin reductase-1 in dysplastic bile ducts and cholangiocarcinoma in a hamster model" pptx

Ngày tải lên : 07/08/2014, 18:21

... ,4002 edixO cirtiN sisenegonicrac detaidem-noitammalfni fo ledom a : htiw detcefni sretsmah ni egamad AND evitartin inirreviv sihcrohtsipO dna evitadixo detaidem-ON fo msinahceM S ihsinawaK ,P nrowahtihtiS ... fo yrtsimehcotsihonummI ylevitcepser ,RxrT eht rof yrtsimehcotsihonummi rof dna noitanimaxe lacigolotsih rof gniniats )E&H( nisoe dna nilyxotameh rof deraperp erew snoitces eussit mµ nehT niffarap ... gniniatnoc lonahtem %001 ni nim 03 rof desremmi erew snoitces eussit revil eht ,noitardyh dna noitaziniffaraped retfA desu saw dohtem )CBA( xelpmoc nitoib nidiva eht ,RxrT fo gniniatsonummi roF...

6
309
0

Báo cáo y học: "Expression analysis of three isoforms of hyaluronan synthase and hyaluronidase in the synovium of knees in osteoarthritis and rheumatoid arthritis by quantitative real-time reverse transcriptase polymerase chain reaction" potx

Ngày tải lên : 09/08/2014, 01:24

... deviation of the baseline of fluorescent signal intensity The CT value of the βactin message was used as an internal standard When the target messages were detected in both the control samples and the ... that the message expression of hyaluronidase-2 in the synovium of OA and RA was approximately four times that in the control synovium This finding suggests that in OA and RA, the protein expression ... volume of hyaluronan from the joint, and the total volume of joint fluid The production of hyaluronan in OA or RA may be decreased because of the reduced expression of HAS-1 and -2 messages The...

7
466
0

Fibroblast-like synovial cells from normal and inflamed knee joints differently affect the expression of pain-related receptors in sensory neurones: a co-culture study ppsx

Ngày tải lên : 09/08/2014, 10:20

... produced the cultures of FLS cells, carried out the ELISA study and collaborated in establishing the co-culture system HGS was involved in planning the study and the preparation of the manuscript ... (chronic AIA) after induction of inflammation in the knee joint, the rats were killed by cervical dislocation during ether anaesthesia In total, 17 untreated rats of the same age and sex were used ... purpose the cells were washed with PBS and incubated for to minutes in PBS containing 0.25% trypsin and 0.02% EDTA (Gibco) Thereafter, the cells were collected, washed with DMEM containing 20% FCS and...

12
184
0

Báo cáo khoa hoc:" An expression of mixed animal model means equations to account for different and variances in the base" pps

Ngày tải lên : 09/08/2014, 18:21

... case Gis the usual inverse of the relationship matrix -’ * o Otherwise, the calculation of G requires computing H introducing the segregation variance between groups and inbreeding, though these ... that the breeding values of the base animals have the same mean and variance, and that the genetic variance of the base generation is twice that of the Mendelian variance In a Gaussian setting, ... vector of Now, calling breeding values of base ) b (a and non-base animals equa- ) r (a and Z l 2] [Z Z the prediction of breeding values when base animals selected is then obtained by solving the...

9
397
0

Báo cáo khoa hoc:"Expression of common fragile sites in two Ceboidea species: Saimiri boliviensis and Alouatta caraya (Primates: Platyrrhini)" pptx

Ngày tải lên : 09/08/2014, 18:21

... diﬀerent bands, no bands signiﬁcantly involved in spontaneous breakage were found in SBO On the other hand, spontaneous breakage analysis in ACA considering a haploid karyotype of 287 bands [21, ... at C-bands Only one ACA c-fra (1q31) is located at a breakpoint of a pericentric inversion involved in the evolution of the Ceboidea karyotype [43] Smeets and Klundert induced fragile site expression ... reported for other heterochromatic regions [50] Another c-fra, 11q15, coincides with a terminal deletion of a C-band in C a nigritus [44] and paracentric inversions observed in C a robustus and C a...

11
241
0

Báo cáo y học: "Serological and molecular expression of Hepatitis B infection in patients with chronic Hepatitis C from Tunisia, North Africa" pdf

Ngày tải lên : 12/08/2014, 01:21

... HBV DNA in the serum The distribution of the studied populations according to these different HBV statuses is shown in Table The status against HBV remained uncertain in the 30 patients and 25 ... patients and controls, the serological and molecular expression of HBV infection markedly differed in the two groups Ongoing HBV infection was found in 12% of controls, most of them expressing HBsAg ... = 2) of patients, respectively Seven patients had mixed infections: 1a and in cases, 1a and 1bin cases and 1a and in one case The viremia levels for HBV and HCV in the studied patients and controls,...

6
516
0

Báo cáo y học: " Epithelial expression of TLR4 is modulated in COPD and by steroids, salmeterol and cigarette smoke" pot

Ngày tải lên : 12/08/2014, 15:21

... airways inflammation and by cigarette smoke, the major pathogenic determinant of COPD A greater understanding of the mechanism of these effects may improve our understanding of the pathogenesis of ... may be the result of disease, smoking or medication TLR4 expression is down regulated in the nasal epithelium of smokers in- vivo We examined expression of TLR4 along with TLR2 and HBD2 in the nasal ... LPS-induced IL-8 expression in the presence of CSE however, and similarly the "protective" effect of salmeterol on dexamethasone-induced inhibition of TLR4 signalling was lost in the presence of...

12
361
0

Báo cáo y học: " Cellular turnover and expression of hypoxic-inducible factor in acute acalculous and calculous cholecystitis" ppt

Ngày tải lên : 13/08/2014, 08:20

... according to the intensity and extent of cytoplasmic and nuclear staining According to this grading system, intense HIF-1α staining was observed in 57% (16/28) of AAC, in 100% (20/20) of ACC and in ... results, and drafting of the manuscript SJ contributed to designing of the study, acquisition of data, and analysis and interpretation of the results KV contributed to designing and acquisition of ... data, and analysis and interpretation of the results SH participated in designing and analysis, and interpretation of the data, as well as in critical revision of the manuscript KT participated in...

6
243
0

Báo cáo y học: "Effects of ischemic pre- and postconditioning on HIF-1a, VEGF and TGF-b expression after warm ischemia and reperfusion in the rat liver" ppsx

Ngày tải lên : 13/08/2014, 13:20

... ischemia and 30 minutes of reperfusion compared to the control group The main finding of this study was an absent of HIF-1a induction in IPC or IPC+IPO treated animals In both of these groups, the expression ... detected corresponding to the point where fluorescent intensity exceeds the background fluorescent intensity, which is 10 × the standard deviation of the baseline The average of triplicates from ... expression of HIF-1 and the degree of apoptosis was increased in rats subjected to 120 of warm liver ischemia compared to non-ischemia [32] Another study supporting the conclusion in the present...

6
328
0

Báo cáo y học: "Genome-wide expression profiling and bioinformatics analysis of diurnally regulated genes in the mouse prefrontal cortex" pps

Ngày tải lên : 14/08/2014, 08:20

... diurnal of the Heat map of enriched TFBSs and their corresponding TFs for each of the eight clusters, when both TFBSs and TFs are present in the diurnal genes The columns indicate the TFs in each of ... calcium/calmodulin-dependent protein kinase IG (Camk1g), Per2, Dbp, splicing factor proline/glutamine rich (Sfpq), oxysterol binding protein-like (Osbpl3), RanBP-type and C3HC4-type zinc finger containing ... of the eight clusters, where the rows represent the enriched (P < 0.05) TFBSs in the kb upstream region of genes in each of the eight clusters The color of the cell represents the degree of matching:...

15
299
0

Báo cáo sinh học: " Expression pattern and polymorphism of three microsatellite markers in the porcine CA3 gene" pptx

Ngày tải lên : 14/08/2014, 13:22

... from the beginning of intron and the novel microsatellite, which included a tandem repeat of (TC)n is located approximately at 1264 bp from the beginning of intron 3.3 Allele frequencies in different ... for fine QTL mapping of production traits and for the study of the expression of porcine CA3 An assessment of the relationships between the CA3 functions and the characterisation of its genomic ... study, the porcine CA3 gene was also differentially expressed in the skeletal muscle of Yorkshire and Meishan pig breeds The results of expression profiles showed that the expression levels of CA3 increased...

13
268
0