incl peptide nucleic acids

peptide nucleic acids

Ngày tải lên : 11/04/2014, 10:02

... transfer from DNA to peptide nucleic acids by template-directed syntheses Nucleic Acids Res 25, 4792–4796 71 Nelson, K E., Levy, M., and Miller, S L (2000) Peptide nucleic acids rather than RNA ... (1999) Peptide nucleic acid A molecule with two identities Acc Chem Res 32, 624–630 12 Nielsen, P E Antisense peptide nucleic acids Curr Opin Mol Ther 2000, 2, 282–287 13 Nielsen, P E (2001) Peptide ... of peptide nucleic acids (PNA) J Peptide Sci 3, 175–183 19 Thomson, S A., Josey, J A., Cadilla, R., Gaul, M D., Hassman, C F., Luzzio, M J., et al (1995) Fmoc mediated synthesis of peptide nucleic...

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Báo cáo khoa học: Molecular dynamics structures of peptide nucleic acidÆDNA hybrid in the wild-type and mutated alleles of Ki-ras proto-oncogene ppt

Ngày tải lên : 07/03/2014, 21:20

... by peptide nucleic acids Nat Genet 15, 212–215 29 Cogoi S, Rapozzi V & Xodo LE (2003) Inhibition of gene expression by peptide nucleic acids in cultured cells Nucleosides Nucleotides Nucleic Acids ... LE (1992) Antisense and antigene properties of peptide nucleic acids Science 258, 1481–1485 Knudsen H & Nielsen PE (1997) Application of peptide nucleic acid in cancer therapy Anticancer Drugs ... (1998) PNA: Synthetic polyamide nucleic acids with unusual binding properties Angew Chem Int Ed Engl 37, 2796– 2893 Nielsen PE (1999) Applications of peptide nucleic acids Curr Opin Biotechnol 10,...

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Nucleic acids DNA & RNA

Ngày tải lên : 13/03/2014, 16:34

... (depending on the sugar they contain) 1- Ribonucleic acids (RNA) The pentose sugar is Ribose (has a hydroxyl group in the 3rd carbon OH) 2- Deoxyribonucleic acids (DNA) The pentose sugar is Deoxyribose ... Certain codons are translated by the cell into certain Amino acids Thus, the sequence of nucleotides in DNA indicate a sequence of Amino acids in a protein ...

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Nucleic acids và tính chất hóa học, hóa sinh học

Ngày tải lên : 13/03/2014, 17:04

... (depending on the sugar they contain) 1- Ribonucleic acids (RNA) The pentose sugar is Ribose (has a hydroxyl group in the 3rd carbon -OH) 2- Deoxyribonucleic acids (DNA) The pentose sugar is Deoxyribose ... Certain codons are translated by the cell into certain Amino acids Thus, the sequence of nucleotides in DNA indicate a sequence of Amino acids in a protein ...

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Sự tiến hóa nucleic acids

Ngày tải lên : 13/03/2014, 17:32

... cellular activity Primary Structure of Nucleic Acids • The primary structure of a nucleic acid is the nucleotide sequence • The nucleotides in nucleic acids are joined by phosphodiester bonds ... particular amino acid • Each of the 20 amino acids needed to build a protein has at least codons • There are also codons that signal the “start” and “end” of a polypeptide chain • The amino acid sequence ... organisms, including humans, have been determined, however, - only primary structure can be determined this way - doesn’t give tertiary structure or protein function mRNA Codons and Associated Amino Acids...

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capillary electrophoresis of nucleic acids, volume i

Ngày tải lên : 10/04/2014, 11:11

... Molecular Biology, Vol 162: Capillary Electrophoresis of Nucleic Acids, Vol 1: Introduction to the Capillary Electrophoresis of Nucleic Acids Edited by: K R Mitchelson and J Cheng © Humana Press ... Molecular Biology, Vol 162: Capillary Electrophoresis of Nucleic Acids, Vol 1: Introduction to the Capillary Electrophoresis of Nucleic Acids Edited by: K R Mitchelson and J Cheng © Humana Press ... Molecular Biology, Vol 162: Capillary Electrophoresis of Nucleic Acids, Vol 1: Introduction to the Capillary Electrophoresis of Nucleic Acids Edited by: K R Mitchelson and J Cheng © Humana Press...

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capillary electrophoresis of nucleic acids, volume ii

Ngày tải lên : 10/04/2014, 11:11

... electrophoresis of proteins and nucleic acids Annu Rev Biophys Biomol Struct 24, 579–610 Olivera, B M., Baine, P., and Davidson, N (1964) Electrophoresis of nucleic acids Biopolymers 2, 245–257 ... S., et al (1994) High-density multiplex detection of nucleic acid sequences: oligonucleotide assay and sequence-coded separation Nucleic Acids Res 22, 4527–4534 19 Day, D J Speiser, P W., White, ... has been recognized for its potential to replace slab-gel electrophoresis for the analysis of nucleic acids (3,4) In particular, the availability of commercial instrumentation for CE over the last...

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nucleic acids

Ngày tải lên : 11/04/2014, 10:00

... electrophoresis or hybridization frequently requires nucleic acid concentrations over mg/mL High molecular weight DNA in a mixture of nucleic acids limits the solubility and interferes with electrophoresis ... large RNA molecules A convenient test substrate for a more sensitive assay is the mixture of nucleic acids extracted from mammalian tissue culture cells labeled for or h with 3H-uridme, which labels ... the stock solution, to Kunitz units/ml in the DNase digestion buffer abcdefg Fig 3H-labeled nucleic acids from HeLa cells after treatment with alkylated DNase and electrophoresis in 0.7% agarose,...

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Báo cáo sinh học: " Discovery of herpesviruses in multi-infected primates using locked nucleic acids (LNA) and a bigenic PCR approach" pdf

Ngày tải lên : 18/06/2014, 18:20

... out in the presence of an additional oligonucleotide modified by the introduction of locked nucleic acids (LNA) (ii) The less conserved glycoprotein B (gB) gene was amplified with degenerate primers ... Singh SK, Rajwanshi VK, Koshkin AA, Dahl BM, Wengel J, Jacobsen JP: The conformations of locked nucleic acids (LNA) J Mol Recognit 2000, 13:44-53 Wang G, Gunic E, Girardet J, Stoisavljevic V: Conformationally ... Moreira BG, Behlke MA, Owczarzy R: Design of LNA probes that improve mismatch discrimination Nucleic Acids Res 2006, 34:e60 Ehlers B, Lowden S: Novel herpesviruses of Suidae: indicators for a second...

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Báo cáo hóa học: " Carbon composite micro- and nano-tubes-based electrodes for detection of nucleic acids" pot

Ngày tải lên : 21/06/2014, 03:20

... Detection of nucleic acids Further, three carbon composite electrodes with different content of carbon particles with various shapes and sizes were prepared and tested on measuring of nucleic acids ... Cyclic voltammetry was used for the detection of two nucleic acids samples mentioned above and the basic electrochemical behaviour of nucleic acids at the surface of the above prepared carbon composite ... MWCNTs had the highest sensitivity to nucleic acids Based on these results, nanocarbon II electrodes were further utilized for detection of both the nucleic acids samples (25 μg/mL) using square...

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báo cáo khoa học: "Self-assembly of proteins and their nucleic acids" docx

Ngày tải lên : 11/08/2014, 00:21

... protein vectors that are capable of self-assembly with nucleic acids The key principle behind our design of the protein vectors is the use of nucleic acids which encode proteins that contain, as part ... recognition is sought, then nucleic acids should in- clude binding sites (i.e specific sequences) for nucleic acid-binding proteins and should also encode corresponding nucleic acid-binding proteins ... (panel A) can routinely and cheaply be included in oligonucleotide primers and thus be easily introduced (in a fully controllable manner) into nucleic acids used for self-assembly Schematic diagram...

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Báo cáo y học: " Contamination of clinical specimens with MLVencoding nucleic acids: implications for XMRV and other candidate human retroviruses" docx

Ngày tải lên : 13/08/2014, 01:20

... contamination of PCR reagents A third report by Sato et al [10] describes the detection of MLV-encoding nucleic acids in PCR reagents obtained from a commercial supplier (Invitrogen) Analyses of individual ... identify three potential sources of contamination in PCR-based studies of XMRV: (i) MLV-encoding nucleic acids present in commercial PCR reagents, (ii) trace amounts of mouse genomic DNA in human blood ... doi:10.1186/1742-4690-7-112 Cite this article as: Smith: Contamination of clinical specimens with MLV-encoding nucleic acids: implications for XMRV and other candidate human retroviruses Retrovirology 2010 7:112...

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Báo cáo y học: "Innate recognition of non-self nucleic acids" ppsx

Ngày tải lên : 14/08/2014, 08:20

... endosomal localization of nucleic acid-specific TLRs is important in preventing their contact with self nucleic acids [5] TLR9 was the first TLR identified to interact with nucleic acids, and its classical ... might exist The induction of non-transcriptional responses to nucleic acids A common feature of the recognition of non-self nucleic acids by TLRs, RLRs and DAI is their ability to induce robust ... receptors Sensing of self nucleic acids can result in autoimmunity Although all the defense mechanisms described above are designed for the recognition of microbial nucleic acids, they can in some...

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Carbon nanotubes and branched DNA based nucleic acid assays towards a PCR free detection and quantification of nucleic acids

Ngày tải lên : 12/09/2015, 09:28

... .1 1.1 Hybridization and Detection of Nucleic Acids 1.1.1 Basic of nucleic acid hybridization .1 1.1.2 Label-free detection of nucleic acids 1.1.2.1 Electrochemical ... polymerase chain reaction (PCR)-free ultrasensitive nucleic acids detection based on signal amplification approach Electrochemical and optical nucleic acids assays based on (I) a novel carbon nanotube ... ultrasensitive electrochemical nucleic acids detection in portable devices, POC and early disease diagnostic applications (II) bDNA-amplified electrochemical nucleic acids assay A novel electrochemical...

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Chương 4: Nucleic acid

Ngày tải lên : 05/10/2012, 08:24

... nên chuỗi polypeptide có tính phân cực: đầu 5’ thường có gốc phosphate đầu 3’ thường có OH tự Nucleic acid gồm hai loại phân tử có cấu tạo giống DNA (desoxyribonucleic acid) RNA (ribonucleic acid) ... nm Tính chất sử dụng để dịnh lượng nucleic acid xác định độ nucleic acid Khi đun nóng dung dịch nucleic acid nhiệt độ cao, thêm acd kiềm để ion hoá base nó, nucleic acid bị biến tính Phân tử DNA ... RNA polymerase khác 4.3 Một số tính chất nucleic acid Dung dịch nucleic acid có độ nhớt cao, có hoạt tính quang học (làm quay mặt phẳng ánh sang phân cực) Nucleic acid hấp thụ mạnh vùng ánh sáng...

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Chương 12: Trao đổi nucleic acid

Ngày tải lên : 05/10/2012, 08:36

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CÂU HỎI TRẮC NGHIỆM ACID NUCLEIC

Ngày tải lên : 24/10/2012, 14:34

... Nucleosid D Nucleotid E Acid nucleic 334 Adenosin là: A PurinB Pyrimidin E Acid nucleic 335 Adenin là: A Base Purin B Base Pyrimidin D Nucleotid C Nucleosid E Acid nucleic 336 Uracil là: A Base ... Pyrimidin D Nucleotid E Acid nucleic 337 ADN ARN là: 106 C Nucleosid Hoá h ọc c huy ển hoá ac id nuc leic Blo c k A PurinB Pyrimidin C Nucleosid D Nucleotid E Acid nucleic 338 Công thức cấu tạo ... deoxyribose, H3PO4 E Guanin, Adenin, Cytosin, Uracil, β.D ribose, H3PO4 288 Thành phần hóa học acid nucleic : Pentose, H3PO4 , Base nitơ Deoxyribose, H3PO4 , Base dẫn xuất từ purin Ribose, H3PO4 ,...

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Báo cáo y học: "Expression of Human Globular Adiponectin-Glucagon-Like Peptide-1 Analog Fusion Protein and Its Assay of Glucose-Lowering Effect In Vivo"

Ngày tải lên : 25/10/2012, 11:10

... 6×His-tag, enterokinase cleavage site (DDDDK), GLP-1-A (31 amino acids) , linker (glycine-rich short peptide, 15 amino acids) , and gAd (137 amino acids) (Fig 1A) The fuhttp://www.medsci.org Int J Med Sci ... terminus of gAd gene The product of this nucleotide sequence was a glycine-rich short peptide including 15 amino acids: [N-(Serine-glycine)7 - Serine-C], which was used as a linker to connect the ... for this analysis The fusion protein was observed in both inclusion body and supernatant But most of them were in inclusion body Lane 1: inclusion body; Lane 2: supernatant http://www.medsci.org...

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Báo cáo y học: " WT1 PEPTIDE VACCINATION IN COMBINATION WITH IMATINIB THERAPY FOR A PATIENT WITH CML IN THE CHRONIC PHASE"

Ngày tải lên : 26/10/2012, 09:39

... peripheral blood (PB) during WT1 peptide vaccination MATERIALS AND METHODS Administration of WT1 peptide Modified-type WT1 peptide (HLA-A*2402restricted, 9-mer peptide; CYTWNQMNL) was synthesized ... clinical y trial using a combi ination of PR peptides ( R1 (peptides derived from prote einase 3) and WT1 peptide in paes tients with myeloid malignancies including AML in d comple remission MDS and ... env peptide (HLA-A*2402-restricted, 9-mer peptide; sequence: RYLRDQQLL)/HLA-A*2402 tetramer were kindly provided by Dr K Kuzushima (Aichi Cancer Center Research Institute) Wild-type WT1 peptide...

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