in vivo in vitro uds assay
Báo cáo sinh học: " Development of an in vitro cleavage assay system to examine vaccinia virus I7L cysteine proteinase activity" pptx
... Effect of4 Figure inhibitors on in vitro processing Effect of inhibitors on in vitro processing Various concentrations of protease inhibitors were added to the in vitro processing assay for hr at ... of inhibitors on in vitro processing Inhibitor Name Concentration Inhibit Cleavage Metalloproteinase Aspartic acid proteinase Serine proteinase Cysteine proteinase EDTA Pepstatin PMSF E-64 E-64 ... profile Processing Kinetics of Core Protein Precursors To determine the optimal temperature and kinetics of processing of the core protein precursors in the in vitro cleavage assay, a time course...
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báo cáo hóa học:" Development of an in vitro cleavage assay system to examine vaccinia virus I7L cysteine proteinase activity" docx
... Effect of4 Figure inhibitors on in vitro processing Effect of inhibitors on in vitro processing Various concentrations of protease inhibitors were added to the in vitro processing assay for hr at ... of inhibitors on in vitro processing Inhibitor Name Concentration Inhibit Cleavage Metalloproteinase Aspartic acid proteinase Serine proteinase Cysteine proteinase EDTA Pepstatin PMSF E-64 E-64 ... profile Processing Kinetics of Core Protein Precursors To determine the optimal temperature and kinetics of processing of the core protein precursors in the in vitro cleavage assay, a time course...
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Báo cáo khoa học: "The response of dehydrated Douglas fir (Pseudotsuga menziesii) pollen to three in vitro viability assays and their relationship to actual fertility" doc
... comparing the effect of hydration on assay response, a paired t-test was used = For in vitro assays, the experimental unit was as a family of pollen grains arising from a single clone including ... each of trees using replicates (bags) per tree Pollination technique was slightly different than described in In vivo fertility In this test, syringe pollinators were used The syringe plunger was ... (1974) for determining significant response differences at the 95% confidence level For pollen lots germinating in the 50% range, 300 grains were observed and for lots germinating either > 90%...
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MECHANISTIC CHARACTERISATION OF DENGUE VIRUS RNA DEPENDENT RNA POLYMERASE NON NUCLEOSIDE INHIBITOR BINDING POCKET THROUGH IN VITRO BIOCHEMICAL ASSAYS AND REVERSE GENETICS ANALYSES
... the inhibitor binding pocket for in vitro polymerase activity and as well for replication fitness in context of the DENV TSV01 infectious virus For these studies, individual amino acids lining ... both finger and palm domains resulting in the closed conformation of RdRp during de novo initiation This is to restrict access to the template binding channel thereby preventing dsRNA binding to ... relevance of the inhibitor binding pocket for in vitro polymerase activity and To assess the relevance of the inhibitor binding pocket for replication fitness in the context of the infectious virus...
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Comparative study of HLA b27 peptide binding specificity by in vitro refolding assay
... HLA-B27 subtype heavy chain and light chain (β2-microglobulin) protein were over-expressed in E coli as an inclusion body and solubilized in 8M Urea The in vitro refolding method was carried out ... moderate influences on binding specificity at the P9 anchor, and most subtypes can bind the same peptides with similar binding affinity In summary, our study has defined some major rules governing ... extended main-chain and side-chain conformations in the binding site Figure 1.6 Interaction between HLA Class I Molecules and Peptides A longitudinal section through the peptide-binding groove...
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Nghiên cứu đánh giá đặc tính nông sinh học của một số dòng hoa đồng tiền (gerbera jamesonii) đột biến tạo được trong nuôi cấy in vitro và in vivo
... n nuụi c y in vitro 4.1.1 44 K t qu nghiờn c u kh nng trỡ c a cỏc d ng ch i cõy ng Ti n t bi n in vitro 4.1.2 44 K t qu nghiờn c u kh nng nhõn nhanh cỏc d ng ng Ti n t bi n in vitro 4.2 49 ... triphosphate BAP : 6-Benzylaminopurine CV : Correlation of Variants D : DMSO : Dimethyl sulphoxide EMS : Ethyl methane sulphonate G : G ch Ki : Kinetin (6-Furfurylaminopurine) LSD : Least Significant ... B ng Kh nng sinh tr ng v nhõn nhanh c a cỏc d ng ch i ng Ti n t bi n in vitro (gi ng hoa mu tr ng) 49 B ng Kh nng sinh tr ng v nhõn nhanh c a cỏc d ng ch i ng Ti n t bi n in vitro (gi ng...
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Tài liệu Báo cáo khoa học: The endogenous retinoid metabolite S-4-oxo-9-cis-13,14-dihydro-retinoic acid activates retinoic acid receptor signalling both in vitro and in vivo pdf
... ⁄ v) fetal bovine serum (Gibco Invitrogen), 1% (v ⁄ v) PEST (Gibco Invitrogen), 1% (v ⁄ v) l-glutamine (Gibco Invitrogen) and 1% nonessential amino acids (Gibco Invitrogen) Murine hepatoma-1 ... were grown in low-glucose DMEM (Gibco Invitrogen) supplemented with 10% (v ⁄ v) fetal bovine serum (Gibco Invitrogen), 1% (v ⁄ v) PEST (Gibco Invitrogen), 1% (v ⁄ v) l-glutamine (Gibco Invitrogen) ... The subsequent incubation with the indicated retinoids was then terminated at the indicated time points by washing with NaCl ⁄ Pi and lysis of the cells using mL of Trizol (Invitrogen) per well...
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Báo cáo khoa học: Activator-binding domains of the SWI ⁄ SNF chromatin remodeling complex characterized in vitro are required for its recruitment to promoters in vivo pot
... mediate interaction with transcriptional activators in vitro [33] Recombinant activator interaction domains interact with activation domains in vitro using a two-step mechanism, where rapid ionic interaction ... function of the increasingly large group of proteins that have been shown to contain intrinsically unstructured regions The intrinsic conformation flexibility that is inherent in this binding mechanism ... activator-binding domains is also of interest in relation to the twostep binding mechanism between Gal4 and Swi1 that has been characterized in vitro [34], and our results suggest that the coupled binding...
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Báo cáo khoa học: Two types of replication protein A in seed plants Characterization of their functions in vitro and in vivo ppt
... checkpoint signaling in vivo It may function in the sensing of DNA damage [22] In contrast with the intensive study of RPA in animals and yeast, little is known about this protein in plants In 1997, ... 3272 We investigated the interaction between OsRPA subunits using yeast two-hybrid analysis (b-galactosidase assay) (Fig 3A) and an in vitro pull-down assay (a protein–protein interaction assay) ... experiment, the indicated proteins were incubated together in NaCl ⁄ Pi containing 250 mM NaCl at °C for h, and then incubated with Talon beads The amount of protein present in the fraction bound...
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Báo cáo khoa học: Insertion of the plant photosystem I subunit G into the thylakoid membrane In vitro and in vivo studies of wild-type and tagged versions of the protein doc
... to leave room for altered protein–protein interactions, which may explain why the Chlamydomonas loop region is exposed to trypsin in the in vitro assay The in vitro import experiments suggested ... trypsin, probed with antibodies directed against PSI-G, PSI-K (intrinsic membrane protein, stromal loop), PSI-O (intrinsic membrane protein, luminal loop) and PSI-D (extrinsic membrane protein, ... disrupting protein–protein interactions within PSI, or by altering the conformation of the loop His-tags were employed in a topological study of the major light harvesting chlorophyll a ⁄ b binding...
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Báo cáo khoa học: Structural studies of the capsular polysaccharide and lipopolysaccharide O-antigen of Aeromonas salmonicida strain 80204-1 produced under in vitro and in vivo growth conditions docx
... and O-chain LPS are composed of linear trisaccharide repeating units containing 3-linked 2-acetamido-2-deoxy-D-quinovose, 4-linked 3-[(N-acetyl-L-alanyl) amido]-3-deoxy-D-quinovose and 3-linked ... O-chain containing L-rhamnose, 2-amino-2-deoxy-D-mannose and D-glucose [11], confirming that the novel CPS and O-chain polysaccharide were produced during the 72-h in vivo culture period In addition, ... determined by the intensity ratio of fragments at m/z 662.2 Da (GalNAcAN-containing trisaccharide repeating unit) and m/z 663.2 Da (GalNAcAcontaining trisaccharide repeating unit) (Fig 4A, inset),...
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Báo cáo khoa học: In vitro and in vivo self-cleavage of Streptococcus pneumoniae signal peptidase I pot
... the protein The self-cleavage of LexA protein is important in the SOS response in bacteria [21–23] In vitro self-cleavage was also observed in all investigated bacterial SPase I including enzymes ... results intrigued us to investigate the cleavage of S pneumoniae SPase I in vivo As shown in Fig 6C, Western blot analysis demostrated that the self-cleavage of SPase I is indeed occuring in vivo in ... explain why self-cleavage is happening in vivo, one speculation is that it may be involved in regulating the activity of the enzyme It is not difficult to imagine that bacteria may secrete proteins...
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Báo cáo khóa học: Determination of modulation of ligand properties of synthetic complex-type biantennary N-glycans by introduction of bisecting GlcNAc in silico, in vitro and in vivo pot
... avoided In the solid-phase assay, saturable and carbohydrate-dependent binding was measured, and the resulting Scatchard plots gave straight lines indicating the presence of a single class of binding ... signal of binding of the fluorescent probe is given as extent of carbohydrate-ligand-dependent binding by subtracting carbohydrate-independent binding from total binding for each cell line BiB10-BSA ... dramatic differences in median fluorescence intensity (Table 2) When the staining profiles were examined using the two neoglycoproteins with/without bisecting GlcNAc, staining intensity was significantly...
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Báo cáo " Isomeranzin against Herpes simplex virus in vitro from Clausena heptaphylla (Roxb.) W. & ARN.: Isolation, structure and biological assay " potx
... These solutions were assayed against HSV-1 and HSV-2 using a cell protection assay Immortalized African Green Monkey Kidney (Vero) cells (ATCC) were grown to a monolayer in minimal essential medimum ... cells were about 40% destroyed At this point in time viral growth had only been observed in cells treated with DMSO and infected with HSV-1 However, in other solvents with 1mg/ml compound, the ... mixture Against HSV-1, it provided about 70% protection, though it was fairly ( 40%) cytotoxic Finally, 0.25 mg/ml in 2.5% ethanol provided about 70% protection against HSV-2 with minor cytotoxic...
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Báo cáo hóa học: " Vaccinia virus lacking the deoxyuridine triphosphatase gene (F2L) replicates well in vitro and in vivo, but is hypersensitive to the antiviral drug " pot
... differences in replication in mice reflect real differences in the biology of these viruses, or are related to differences in the animal models including route of infection In HSV, direct intracranial inoculation ... evaluated in cell culture and in intranasally infected mice The deletion of F2L had a minimal impact on viral replication in vitro and did not appear to significantly reduce the virulence in vivo ... replication in vivo In HSV, deletion of the dUTPase homolog did not effect the replication of the virus in vitro, however its virulence was reduced by 1000-fold in mice following footpad inoculation...
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Báo cáo hóa học: " N-methylisatin-beta-thiosemicarbazone derivative (SCH 16) is an inhibitor of Japanese encephalitis virus infection in vitro and in vivo" ppt
... Screening for inhibition of virus induced cytopathic effect in vitro The antiviral activity assay of the Ribavarin and MIBT derivatives against JEV, Den-2 virus or WNV were screened in vitro using ... depicting the in vivo effect of SCH 16 against a lethal JEV challenge Survival graphs depicting the in vivo effect of SCH 16 against a lethal JEV challenge SCH 16 was administered to swiss albino ... from infected cells that were not treated with SCH 16 showed the presence of JEV proteins (Figure 4) In vivo evaluation of compounds against JEV using mouse model After ascertaining the in vivo...
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Báo cáo hóa học: " Inhibition of foot-and-mouth disease virus replication in vitro and in vivo by small interfering RNA" pot
... J, Ince N, Ouyang N, Min J, Chen J, Shankar P, Lieberman J: RNA interference targeting Fas protects mice from fulminant hepatitis Nat Med 2003, 9:347-351 Gitlin L, Karelsky S, Andino R: Short interfering ... grant from the Bingtuan Doctor Foundation Program (05JC04, XinJiang, China) References survival 40% 11.1% survival Competing interests 5LD50 20LD50 FMD3 in this study, siRNAs targeting highly conserved ... http://www.virologyj.com/content/5/1/86 ously injected in the neck with 50–100 ug of plasmids dissolved in 100 ul of saline Mice of the control group were subcutaneously injected with saline After h, the suckling mice were...
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báo cáo hóa học:" Functional significance of the signal transduction pathways Akt and Erk in ovarian follicles: in vitro and in vivo studies in cattle and sheep" ppt
... important in mediating the effects of FSH on inhibin-A, activin-A, oestradiol and progesterone secretion and also important in mediating IGF-I stimulated inhibin-A, activin-A, follistatin, oestradiol ... immunization of sheep against an amino terminal peptide of the inhibin αC subunit on intrafollicular levels of activin A, inhibin A and follistatin Journal of Endocrinology 1998, 157:157-168 ... Figure activin-A and follistatinin vitro with control medium, FSH cell number and theper replicate)oestradiol,combination inhibin-Atreating granulosa cells (N = replicates with wells included per...
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báo cáo hóa học: " In vitro and in vivo pre-clinical analysis of a F(ab’)2 fragment of panitumumab for molecular imaging and therapy of HER1-positive cancers" pot
... distribution in these last two studies was similar to what was obtained with the i.v injected 11 1In- labeled panitumumab F(ab’)2 already discussed Discussion The in vivo and in vitro properties of the intact ... point Imaging g-Scintigraphy was performed with tumor bearing mice to further validate the 111 In- CHX-A"-panitumumab F(ab’)2 tumor targeting Imaging studies were performed with s.c tumor bearing ... the i.v injected group and 19.3 h for the i.p.-injected group In contrast to the i.v.- injected sets of mice, the clearance (T1/2b phase) of the RIC following i.p injection in the mice bearing i.p...
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Báo cáo hóa học: " CdTe quantum dots with daunorubicin induce apoptosis of multidrug-resistant human hepatoma HepG2/ADM cells: in vitro and in vivo evaluation" pptx
... cultured in the cell culture medium containing μg/ mL adriamycin (Sigma) Both cell lines were maintained in RPMI-1640 medium containing 10% FCS, 100 U/ml of penicillin, and 100 μg/ml of streptomycin ... fixed in 100% methanol for 10 Cell monolayers were blocked in 5% BSA in PBS for 45 and incubated for h at room temperature with P-gp antibodies (Invitrogen, Beijing, China), followed by incubation ... from the Institute of Hematology of Tianjin, Chinese Academy of Medical Sciences (Tianjin, China) To develop the drugresistant cell line (HepG2/ADM), adriamycin was added to HepG2 cells in a stepwise...
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Tìm thêm: xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ lồng sóc các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25