in vivo delivery of sirna to murine retinal capillary endothelial cells by large volume hydrodynamic injection

Báo cáo khoa hoc:" Biodegradable Nanoparticles are Excellent Vehicle for Site Directed in-vivo Delivery of Drugs and Vaccines" pptx

Ngày tải lên : 11/08/2014, 08:20

... Vehicle for Site Directed in- vivo Delivery of Drugs and Vaccines Anil Mahapatro1 and Dinesh K Singh2* Bioengineering Program & Department of Industrial and Manufacturing Engineering, Wichita State University, ... hardening of the droplets of emulsified gelatin solution into gelatin NPs The gelatin emulsion droplets are cooled below the gelation point in an ice bath leading to gelation of the droplets [19] into ... high loading ability of NPs reduces the amount of the polymer carrier required for vaccine/drug delivery in the body The loading of drugs/vaccine into/onto nanoparticles is achieved by two methods:...

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Báo cáo y học: " The connection domain in reverse transcriptase facilitates the in vivo annealing of tRNALys3 to HIV-1 genomic RNA" potx

Ngày tải lên : 13/08/2014, 13:20

... thumb domain in GagPol in tRNALys3 viral packaging tRNALys3 incorpora- tion into HIV-1 is not affected by deletion of the IN domain in GagPol, nor by further deletion of the RNaseH Page of (page ... tion The data in the right side of panel A indicate that Cterminal deletions of GagPol extending into the connection domain result in an 85% or greater decrease in the initiation of reverse transcription ... annealing, while mutations in NC sequences within GagPol not, indicating the importance of Gag NC sequences in the annealing [16] In vitro, Gag has been reported to facilitate tRNALys3 annealing to...

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Báo cáo khoa học: Efﬁcient and targeted delivery of siRNA in vivo pdf

Ngày tải lên : 06/03/2014, 01:23

... desirable in siRNA delivery in vivo than synthetic polymers Targeted siRNA delivery in vivo In order to achieve RNAi in vivo via systemic delivery, it is crucial for siRNA to be efficiently located in ... challenges in achieving efficient and targeted siRNA delivery in vivo Major hurdles in siRNA delivery in vivo and various approaches to overcoming them are illustrated in Fig Local versus systemic delivery ... FEBS 4815 In vivo siRNA delivery M S Shim and Y J Kwon Fig Interdisciplinary approaches to achieving efficient and targeted RNAi in vivo by overcoming multiscale barriers in systemic siRNA delivery...

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Báo cáo khoa học: A steady-state modeling approach to validate an in vivo mechanism of the GAL regulatory network in Saccharomyces cerevisiae ppt

Ngày tải lên : 07/03/2014, 16:20

... sequences of the GAL genes through its N-terminal DNA binding site Gal80p inhibits the transcriptional activity of Gal4p by binding to its 28 amino acid region at the carboxyl terminal [25–27] In vitro ... mechanism of induction of GAL genes by galactose In each of the models, cytoplasmic Gal3p is activated by galactose Further, Gal4p dimerizes and interacts with the DNA to form the DNA–Gal4p complex in ... constant for activation of Gal3p by galactose and t refers to the total component concentration The expression of GAL genes is determined by the binding of the operator to either the dimer Gal4p...

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Báo cáo sinh học: " Actively replicating West Nile virus is resistant to cytoplasmic delivery of siRNA" ppt

Ngày tải lên : 19/06/2014, 08:20

... WNV Lineage I WNV Lineage I WNV Lineage I WNV Lineage I WNV Lineage I WNV Lineage I WNV Lineage II WNV Lineage I WNV Lineage I WNV Lineage I WNV Lineage I WNV Lineage I WNV Lineage I WNV Lineage ... vesicles induced by WNV To determine if the mode of delivery of siRNA affected RNAi resistance, we tested whether siRNA could inhibit replication of a persistently replicating subgenomic lineage ... active in WNV infected cells RNAi is RNAi is active in WNV infected cells RNAi of influenza M2 gene in cells that replicate WNV RNA Huh7.5, Huh7.5Rep, and WNV infected Huh7.5 cells (8 hours post infection)...

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báo cáo hóa học:" Actively replicating West Nile virus is resistant to cytoplasmic delivery of siRNA" pptx

Ngày tải lên : 20/06/2014, 04:20

... WNV Lineage I WNV Lineage I WNV Lineage I WNV Lineage I WNV Lineage I WNV Lineage I WNV Lineage II WNV Lineage I WNV Lineage I WNV Lineage I WNV Lineage I WNV Lineage I WNV Lineage I WNV Lineage ... vesicles induced by WNV To determine if the mode of delivery of siRNA affected RNAi resistance, we tested whether siRNA could inhibit replication of a persistently replicating subgenomic lineage ... active in WNV infected cells RNAi is RNAi is active in WNV infected cells RNAi of influenza M2 gene in cells that replicate WNV RNA Huh7.5, Huh7.5Rep, and WNV infected Huh7.5 cells (8 hours post infection)...

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báo cáo hóa học:" An in vivo evaluation of bone response to three implant surfaces using a rabbit intramedullary rod model" doc

Ngày tải lên : 20/06/2014, 04:20

... carrier gas to produce coating with thickness ranging from 40-70 microns (nominal 50 microns) The coating had a minimum total crystallinity of 65% The minimum HA fraction in the crystalline phase ... hydroxyapatite coating is the dominant factor affecting in vivo osseointegration One study concluded that surface roughness contributed more to increased bone apposition rates than hydroxyapatite coating [26] ... of the implants were counted Osseointegration was defined as bone -to- implant contact and calculated as the ratio of the number of bone pixels relative to the total number of pixels (bone + soft...

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Báo cáo toán học: "OXIDATIVE PHOSPHORYLATION: Kinetic and Thermodynamic Correlation between Electron Flow, Proton Translocation, Oxygen Consumption and ATP Synthesis under Close to In Vivo Concentrations of Oxygen" docx

Ngày tải lên : 08/08/2014, 17:20

... determined by adding 55 nmols of O to a fully reduced sample of mg of mitochondrial protein Every unit in the y-axis represents 3.37 nmols of H+ [18, 19] The synthesis of ATP was initiated by adding ... different states of reduction and/or protonation In Fig.1a the process of oxidative phosphorylation is initiated by adding 300 nmols ADP to mitochondria respiring in state-4 in the presence of ~230 μM ... performed in oxygen-pulse experiments by adding O2 to fully reduced samples of RLM The uptake of O2 and the changes in the redox state of cytochrome aa3 were simultaneously initiated by adding 9.2...

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Báo cáo y học: "The in vivo expression of actin/salt-resistant hyperactive DNase I inhibits the development of anti-ssDNA and anti-histone autoantibodies in a murine model of systemic lupus erythematosus" ppt

Ngày tải lên : 09/08/2014, 07:20

... number of factors, including the longevity of the injected protein and the level of free G-actin in the circulation at sites of inflammation inhibiting the DNase I activity Our data obtained by a ... model of autoimmunity Transient autoimmunity can be induced in mice by the administration of a single high dose of LPS This induces a pulse of nucleosomes and chromatin in the plasma within the ... number of DNase I mutants, including ones Page of 11 (page number not for citation purposes) resistant to inhibition by G-actin Mutations were also introduced to increase the affinity of the...

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Báo cáo y học: "Discrepancy between the in vitro and in vivo effects of murine mesenchymal stem cells on T-cell proliferation and collagen-induced arthritis" doc

Ngày tải lên : 12/08/2014, 11:23

... with IL-17 to upregulate expression of PD-L1, iNOS, and COX-2 in MSCs, making these molecules candidate mediators of T-cell inhibition The involvement of iNOS and COX-2 in inhibition of T-cell ... demonstrated by the addition of inhibitors of these enzymes - GW274150 and indomethacin [8,36], respectively - to the co-cultures The addition of these inhibitors resulted in the abrogation of the inhibition ... cytokines The serum of mice was pooled per group and analyzed for the T-cell cytokines IL-2, IL-5, IL-6, IL-10, and IFN-g The injection of anti-CD3 antibody caused a profound increase in cytokine...

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Báo cáo y học: "Attenuation of fibrosis in vitro and in vivo with SPARC siRNA" ppsx

Ngày tải lên : 12/08/2014, 12:20

... reduced by their corresponding siRNAs (P = 0.002 and 0.0004, respectively) siRNAs of SPARC and Ctgf ameliorated fibrosis in skin and reduced inflammation in lungs induced by bleomycin HE stains of ... Injection with saline (negative control) only; B Injection with bleomycin only; C Injection with bleomycin and treatment with SPARC siRNA; D Injection with bleomycin and treatment with Ctgf siRNA ... Quantification of total collagen in skin samples with the Sircol assay showed a 2.2-fold increase in bleomycininduced skin compared with saline-injected skin (P = 0.050) Ctgf siRNA treatment...

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Báo cáo y học: " In vivo imaging of the airway wall in asthma: fibered confocal fluorescence microscopy in relation to histology and lung function" potx

Ngày tải lên : 12/08/2014, 13:22

... excitation by an external laser light source The laser light is guided through a bundle of optical microfibres to the tip of the miniprobe of mm in diameter, which can be inserted into the working channel ... However, to our knowledge this is the first study to investigate the histological substrate of autofluorescence in 488 nm FCFM images using in vivo human endobronchial biopsy specimens obtained from ... main carina was chosen as the location for FCFM and biopsy to minimize imaging artefacts resulting from inadequate positioning of the miniprobe superimposed on the movement of the airways due to...

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Báo cáo y học: "In-vivo evaluation of simultaneous administration of incompatible drugs in a central venous catheter with a decreased port to port distance" pps

Ngày tải lên : 12/08/2014, 18:20

... length of cm, for the in vivo administration of incompatible solutions, phenytoin and TPN, using a swine model, did not lead to precipitates large enough to cause adverse clinical effects in our ... USA) was administered to all animals (n = 10) through the distal lumen at the usual maintenance dose of 2.5 mg/kg/dose at a rate of mg/kg/min up to a maximum of 50 mg/min via a syringe infusion ... distal to the first sampling site An 18-gauge catheter with a three-way stop-cock was inserted at each sampling site (Fig 1) Hematocrit was between 35% and 45% during the experiment Phenytoin and total...

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Báo cáo y học: " A remission spectroscopy system for in vivo monitoring of hemoglobin oxygen saturation in murine hepatic sinusoids, in early systemic inflammation" doc

Ngày tải lên : 13/08/2014, 13:20

... reduction in hepatic sinusoidal HbsO2 during the early stages of systemic inflammation In parallel, we detected an increasing NAD(P)H autofluorescence representing an intracellular inadequate ... Quantification of redox state and cell death was performed off-line by frame -by- frame analysis of the videotaped images using Meta Imaging Series Software (Ver 6.1; Universal Imaging Corp., Downington, ... are accompanied by increasing markers of liver cell injury Future therapeutic interventions should focus on the amelioration of sinusoidal HbsO2 followed by an improvement in mitochondrial redox...

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The development of an in vivo humanized mouse model to investigate epstein barr virus infection and tumorigenesis

Ngày tải lên : 10/09/2015, 09:22

... mode of transmission is by inoculation of infected saliva EBV infects B lymphocytes by engaging its envelope protein gp350/220 with CD21 (complement receptor 2) The invasion of the virus into non-B ... proteins disturb the cytokine balance of the disease microenvironment to favor the survival of infected host cells EBV encodes cytokine homologs and regulatory factors to manipulate the host cytokine ... described by the first model and the high level of viremia in the germinal centers in infectious mononucleosis causes the infection of bystander germinal center cells, pushing them into the latency...

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In vitro and in vivo evaluation of transferrin conjugated lipid shell and polymer core nanoparticles for targeted delivery of docetaxel

Ngày tải lên : 09/10/2015, 11:18

... microtubules, an essential part of the cytoskeleton of eukaryotic cells The vinca alkaloids prevent the protein from polymerizing into microtubules by binding specifically to β-tubulin In contrast, the taxanes ... multiplying hair follicle and stomach lining cells will also be affected, resulting in side effects like hair loss and stomach upset In radiation therapy, certain types of energy are utilized to shrink ... need of extravasation of nanocarriers to arrive to their targeted site, (ii) the binding to their receptors is directly possible after intravenous injection, (iii) the potential risk of emerging...

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In vitro and in vivo investigation of nanoparticles of a novel copolymer for substained and controlled delivery of docetaxel

Ngày tải lên : 09/10/2015, 11:18

... can be subdivided into two categories, namely intrinsic and extrinsic factors Intrinsic factors mainly include the genetic make up of the body, which cannot be controlled by the individuals For ... parts of the body The most common kind of enzyme involved in this degradation of drugs is cytochrome P450 (or CYP), mainly located in liver and intestine CYP is a large family of hemoproteins which ... present in physiological system is the high probability of binding to endogenous proteins in the circulatory system The high-binding affinity of most commercial formulations to plasma proteins reduces...

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In vitro and in vivo study of vitamin e TPGs coated immunoliposomes for sustained and targeted delivery of docetaxel

Ngày tải lên : 09/10/2015, 11:18

... reversibly to the β- subunit of tubulin in the microtubules rather than to tubulin dimers The binding site to tubulin differs from the one of vinca-alkaloids and podophyllotoxins While vincas inhibit ... present in physiological system is the high probability of binding to endogenous proteins in the circulatory system The high-binding affinity of most commercial formulations to plasma proteins reduces ... produced by Streptomyces strain of bacteria (Lomovskaya et al., 1999) This type of drug is believed to intercalate into DNA, thus preventing the growth of cancer cells due to the inhibition of enzymes...

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Stability and in vivo evaluation of pullulan acetate as a drug nanocarrier

Ngày tải lên : 23/04/2013, 21:38

... were fixed in 10% phosphate buffered formalin, embedded in paraffin, sectioned, and stained with hematoxylin and eosin (H&E) In vivo pharmacokinetics and bioavailability In vivo pharmacokinetic study ... Drug Delivery Downloaded from informahealthcare.com by University of Technology Sydney on 03/01/11 For personal use only In vivo toxicity The toxicity of PANs was evaluated in vivo according to ... personal use only In vivo toxicity To evaluate whether i.v administration (at dose of 200 mg/kg) of PANs was associated with any toxicity in vivo, animals were treated with a single dose of empty NPs...

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Tài liệu Guidelines for the appointment of General Practitioners with Special Interests in the Delivery of Clinical Services pdf

Ngày tải lên : 14/02/2014, 21:20

... training • Base line experience working as a clinical assistant e Evidence of maintenance of competencies The GPwSI would be expected to maintain his or her competencies through continued professional ... Demonstration of ability to work in teams by evidence of taking part in multidisciplinary teamwork to plan and deliver service provision and individual patient care • Delivering multi- and uniprofessional ... them to bring about improvement in delivery of sexual health services Understanding of primary care structures and how these may affect delivery of services within the PCO Understanding of service...

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