in vitro studies of liposome mediated gene transfection
Báo cáo khoa học: Bridging the gap between in silico and cell-based analysis of the nuclear factor-jB signaling pathway by in vitro studies of IKK2 ppt
Ngày tải lên :
16/03/2014, 11:20
... (Fig 3E) Interestingly, the inclusion of IKK2 inhibition by SC-514 with our experimentally determined rate constants in the original model resulted in a delay in simulated peak and damping of subsequent ... simulated in silico Combining this method of analysis (in vitro, cell-based and in silico analysis) will facilitate systematic understanding of the underlying properties of this signaling pathway ... = Intensity of nucleus Intensity of cytoplasm Intensity of nucleus Scheme This is a simplication of a cell as seen by analysis software, where the software measures the intensity of NF-jB in...
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In vivo and in vitro studies of th17 response to specific immunotherapy in house dust mite induced allergic rhinitis patients
Ngày tải lên :
23/09/2015, 14:47
... in control subjects; while the increase of Th2 but decrease of Th1 cells was also determined in all AR’s PBMCs These results show a concordance between in vivo and in vitro findings, confirming ... markers in each study group tested in vitro 3.2 In vivo Study 3.3.1 Th17 specific gene mRNA/protein expression Following a 3-day in vitro stimulation, there were mRNA/protein levels of Th17 associated ... treatment were used in the following in vivo and in vitro studies 3.3 In vitro Study To mimic the functional response of Th17 and other Th cell subpopulations to HDM allergens, an in vitro allergen...
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Báo cáo khoa học: Insertion of the plant photosystem I subunit G into the thylakoid membrane In vitro and in vivo studies of wild-type and tagged versions of the protein doc
Ngày tải lên :
07/03/2014, 21:20
... disrupting protein–protein interactions within PSI, or by altering the conformation of the loop His-tags were employed in a topological study of the major light harvesting chlorophyll a ⁄ b binding ... altered protein–protein interactions, which may explain why the Chlamydomonas loop region is exposed to trypsin in the in vitro assay The in vitro import experiments suggested that the loop of Arabidopsis ... shown in Fig 4C Interestingly, both tags are recognized more efficiently in the context of the PSI-G loop region than at the C-terminus Topology of PSI-G in vivo In order to examine the topology of...
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Báo cáo khoa học: Transcriptional regulation of the desferrioxamine gene cluster of Streptomyces coelicolor is mediated by binding of DmdR1 to an iron box in the promoter of the desA gene doc
Ngày tải lên :
16/03/2014, 11:20
... expression of two genes of Streptomyces pilosus involved in the biosynthesis of siderophore desferroxamine B Gene 64, 179–188 Tao X & Murphy JR (1992) Binding of the metalloregulatory protein DtxR ... Regulation of the desferrioxamine gene cluster B Fig Binding of the DmdR1 protein to the desA promoter region of either S pilosus (left) or S coelicolor (right) at increasing protein concentrations ... TAgPuPuT [19] It is interesting that the )10 sequence is located inside the iron box of desA (nucleotides 7–12 of the iron box), explaining the regulation of desA expression by binding of DmdR1 to the...
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báo cáo hóa học:" In vitro suppression of the MMP-3 gene in normal and cytokine-treated human chondrosarcoma using small interfering RNA" doc
Ngày tải lên :
20/06/2014, 04:20
... metalloproteinases mediating matrix degradation in interleukin 1-stimulated human articular cartilage Matrix 1993, 13:95-102 Vincenti MP, Clark IM, Brinckerhoff CE: Using inhibitors of metalloproteinases ... have definite effects on NO production in the joints These inhibitory effects of HA on cell apoptosis may play a role in its mechanism of action in chondroprotection [38] Our findings indicated ... 90 min, and terminated by heat inactivation at 70°C for 15 Measurement of GAG levels The level of GAG appearing in the medium of explants, cell cultures, and papain-digested cells was determined...
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báo cáo hóa học:" Activation of PKA, p38 MAPK and ERK1/2 by gonadotropins in cumulus cells is critical for induction of EGF-like factor and TACE/ ADAM17 gene expression during in vitro maturation of porcine COCs" doc
Ngày tải lên :
20/06/2014, 07:20
... P450 gene (CYP11A1) revisited: binding of GATA, cyclic adenosine 3', 5'-monophosphate response element-binding protein and activation protein (AP)-1 proteins to a distal novel cluster of Page of ... ovulation in mouse [9-11] Since the suppression of the EGF receptor tyrosine kinase activity by specific inhibitor completely suppressed cumulus expansion and meiotic maturation of oocytes during in vitro ... expression in cumulus cells during meiotic resumption of porcine oocytes Endocrinology 2005, 146:186-194 Shimada M, Anas MKI, Terada T: Effects of phosphotidylinositol 3-kinase inhibitors, wortmannin...
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báo cáo hóa học:" Microarray analysis of Foxl2 mediated gene regulation in the mouse ovary derived KK1 granulosa cell line: Over-expression of Foxl2 leads to activation of the gonadotropin releasing hormone receptor gene promoter" pptx
Ngày tải lên :
20/06/2014, 07:20
... activity of the Mad-SID is mediated through interactions of the Mad N-terminus with the mammalian Sin3 co-repressor protein [10,11] The effectiveness of the Sin3 binding domain of Mad in silencing gene ... DNA binding proteins has been demonstrated in two studies One group fused the Mad SID to the c-Jun DNA binding domain and demonstrated specific inhibition of transcription mediated by binding ... follicles in sheep [32] Serpine2 protein levels are elevated in dominant bovine follicles [33], whereas the levels of Serpine2 are lower in ovaries of Foxl2 knockout mice suggesting that the gene is induced...
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Báo cáo khoa học: "In vitro studies on the modification of low-dose hyper-radiosensitivity in prostate cancer cells by incubation with genistein and estradiol" pot
Ngày tải lên :
09/08/2014, 09:22
... use of FCS-containing media during genistein incubation and the incubation with higher genistein doses Mechanisms of interaction To search for mechanisms of interaction we investigated protein ... Genistein, a tyrosine kinase inhibitor, enhanced radiosensitivity in human esophageal cancer cell lines in vitro: possible involvement of inhibition of survival signal transduction pathways Int ... after incubation with genistein concentrations of 15 – 30 μM in FCS-containing media [41] In this study the NF-κB activity was investigated, too An inhibition of radiationinduced activation of NF-κB...
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Báo cáo khoa học: "In vitro dynamics of HIV-1 BF intersubtype recombinants genomic regions involved in the regulation of gene expression" pdf
Ngày tải lên :
12/08/2014, 04:21
... provides useful data on the consequences of HIV-1 intersubtype recombination, opening a window to better understand its role in generating novel strains and inducing viral fitness changes, which would ... found in the LTR, since it is well documented that transactivation of HIV-1 gene expression is mainly induced by Tat through the interaction with TAR, and by the interaction of Vpr with cis-acting ... factor binding sites present in the LTR revealed a high conservation of most of them Nevertheless, a remarkable high frequency of sequences harboring mutations in the NFkβ and Sp1 binding sites...
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Báo cáo khoa học: " In vitro dynamics of HIV-1 BF intersubtype recombinants genomic regions involved in the regulation of gene expression" docx
Ngày tải lên :
12/08/2014, 04:22
... provides useful data on the consequences of HIV-1 intersubtype recombination, opening a window to better understand its role in generating novel strains and inducing viral fitness changes, which would ... found in the LTR, since it is well documented that transactivation of HIV-1 gene expression is mainly induced by Tat through the interaction with TAR, and by the interaction of Vpr with cis-acting ... factor binding sites present in the LTR revealed a high conservation of most of them Nevertheless, a remarkable high frequency of sequences harboring mutations in the NFkβ and Sp1 binding sites...
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In vitro studies on the color stability and masking ability of composite cores and the influence of posts and cores on the shades of all ceramic systems
Ngày tải lên :
09/10/2015, 11:24
... an increase in scattering within the material Light scattering in a material is the result of scattering centers that cause the incident light to be scattered in all directions The scattering ... rate of 94% in a five year clinical study27 This crown system is composed of a densely sintered, high-purity aluminum oxide coping that is combined with the low-fusing AllCeram veneering porcelain17 ... cores in masking the color of prefabricated posts 61 Figure 4i The ability of 1.0 mm thick composite cores in masking the color of prefabricated posts Figure 4j Influence of the color of prefabricated...
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Analysis, sequencing and in vitro expression of PCR products
Ngày tải lên :
25/10/2013, 22:20
... functionally analyzed using in vitro assays Furthermore, rapid in vitro protein–protein interaction studies could be performed using this technique where not only full-length version of proteins could be ... also protein fragments for mapping of interaction domains The use of in vitro expression approaches to analyze protein products is likely to gain momentum for the analysis of known proteins as well ... strategies The in vitro expression of proteins can also be used for functional studies of proteins For example, enzymes synthesized in vitro are likely to be correctly folded and to retain enzymatic...
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Báo cáo khoa học: Nuclear actin and actin-binding proteins in the regulation of transcription and gene expression docx
Ngày tải lên :
07/03/2014, 01:20
... Role of nuclear actin-binding proteins interacting with the androgen receptor AR, androgen receptor; LBD, ligand-binding domain Actin-binding protein Targeting sequence Gelsolin Direct or indirect ... Cross-linking proteins Filamin A NLS? Cross-linking proteins Transgelin ()) Cross-linking proteins Functions as scaffolds for signaling intermediates that stimulate actin elongation; binding partners ... actin-binding site located in the hnRNP U C-terminus and associate with the phosphorylated C-terminal domain of Pol II [43] Injection of a peptide acting as a competitive inhibitor of protein–protein...
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Báo cáo khoa học: In vitro expansion of DNA triplet repeats with bulge binders and different DNA polymerases pdf
Ngày tải lên :
07/03/2014, 06:20
... reaction by using 32P-labeling primer or template in the presence or absence of DNA-binding agents (DDIs and doxorubicin) The DNA bulge binding of both compounds was detected by CD and UV melting experiments ... is formed To gain insight into the binding of drugs to bulge DNA, several bulgecontaining oligonucleotides were selected as binding hosts for DDI-1A and DDI-1B CD spectroscopy of DDI-1A (Fig ... two-base bulge in DNA via stacking of its helically oriented aromatic ring systems on the bulge-flanking base pairs that define the long sides of the triangular prism binding pocket, with its amino sugar...
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Báo cáo khoa học: The in vitro effects of CRE-decoy oligonucleotides in combination with conventional chemotherapy in colorectal cancer cell lines potx
Ngày tải lên :
07/03/2014, 15:20
... alone did not increase the extent of staining However, in cells that had been cocultured with CDO and a cytotoxic drug, staining was significantly increased, indicating the presence of senescence ... percentage of each individual control In summary, these data provide a possible model of drug interaction in GEO cells, which involves the complex interaction of proteins involved in cell cycle regulation ... cells, resulted in the absence of enhanced cell growth inhibition The central role of p21waf1 in mediating senescence is currently being investigated in isogenic cell lines by gene expression...
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Báo cáo khoa học: In vivo studies of altered expression patterns of p53 and proliferative control genes in chronic vitamin A deficiency and hypervitaminosis pot
Ngày tải lên :
08/03/2014, 08:20
... dithiothreitol in a Perkin-Elmer GeneAmp 9700 thermal cycler at 25 °C (10 min), 50 °C (60 min) and 70 °C (15 min) The PCR was started by adding lL of the cDNA solution to a mixture containing 9.95 lL of ... protein extracts were obtained as described in experimental procedures A single band of about 53 kDa was detected in liver and lung indicating that the amount of p53 protein was significantly increased ... role of vitamin A status in the expression of genes related to the regulation of cell proliferation has been studied in vivo and in vitro, and shows that retinoids play an important role in proliferation...
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In Vitro Screening of Plant Resources for Extra-Nutritional Attributes in Ruminants: Nuclear and Related Methodologies pptx
Ngày tải lên :
15/03/2014, 04:20
... challenges of extrapolating in vitro findings to in vivo evaluation of plant resources The chapters in this book are written by experts interested in exploring and making better use of plant biodiversity ... the initial screening is conducted in vitro because of the large number of candidate plant species and the prohibitive cost of screening them in vivo The number of species for in vivo testing ... remaining larvae into individual labelled petri dishes and stain with a few drops of helminthological iodine 10 Add 50 μL of helminthological iodine to each well on the culture plate 11 Using...
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Báo cáo khoa học: In vitro analysis of the relationship between endonuclease and maturase activities in the bi-functional doc
Ngày tải lên :
17/03/2014, 10:20
... stringent concentration of KCl, there was a correlation between inhibition of endonuclease activity and inhibition of splicing activity indicating that RNA binding can be monitored by measuring ... and its binding affinity is not altered by the presence of DNA in the DNA binding site Interestingly, when nM RNA and 10 nM DNA were added simultaneously to a limiting amount of protein in TK8 buffer ... background In vitro competition assays RNA splicing and DNA cleavage competition experiments both involved a prebinding step in which either DNA or RNA substrates were incubated with I-AniI in a binding...
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Báo cáo khoa học: Chick embryo anchored alkaline phosphatase and mineralization process in vitro Influence of Ca 2+ and nature of substrates doc
Ngày tải lên :
23/03/2014, 17:21
... in the GPI-bALP mediated mineralization: effects of Ca2+ concentration To assess the role of Ca2+ in initiating the mineralization process, we modelled the biological process by using 0.8 U of ... presence of anchored GPI-bALP (Fig 1) The presence of HA in the mineral samples was confirmed by In uence of other phosphate substrates in the mineralization process To examine the effects of substrates ... of HA No mineral material was formed in media lacking GPI-bALP Mineral calcium phosphate identification by infrared spectra To identify the quality and the type of mineral formation obtained in...
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Báo cáo khoa học: Membrane compartments and purinergic signalling: the role of plasma membrane microdomains in the modulation of P2XR-mediated signalling pot
Ngày tải lên :
30/03/2014, 02:20
... interaction of Ras with caveolin, an integral membrane protein of caveolae microdomains Detergent-free purification of caveolae microdomains J Biol Chem 271, 9690–9697 53 Smart EJ, Ying YS, Mineo ... regulators of G-protein signaling (RGS) 16 function I Mutation of amino-terminal cysteine residues on RGS16 prevents its targeting to lipid rafts and palmitoylation of an internal cysteine residue ... than those in the lipids of the surrounding membrane In fact, this fatty acid composition favors a tighter packing of phospholipids and cholesterol, increasing the rigidity of these domains [32–35]...
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