in vitro derivation and expansion of endothelial cells from embryonic stem cells pdf

Báo cáo khoa học: In vitro selection and characterization of a stable subdomain of phosphoribosylanthranilate isomerase potx

Ngày tải lên : 30/03/2014, 20:20

... 000 of 87 of 68 of 87 11 of 87 38 of 87 < 1.1 < 1.5 2.3 13 44 arginine of the new epitope, and removed the fragment of the PRAI (ba)8-barrel corresponding to a6(ba)7)8 The remaining fragment of ... contributing to one of the hydrogen bonds in the core of the barrel, Lys258 (b1) and Gln332 (b4), remain in trPRAI It is tempting to speculate that, in the absence of the salt bridge linking Lys258 and ... signiﬁcantly perturbing the folding of the underlying (ba)8-barrel This was investigated by comparing the far-UV and near-UV CD spectra of PRAI and FLAG-PRAI in a buffer in which PRAI retains catalytic...

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Báo cáo y học: " Influenza H5N1 virus infection of polarized human alveolar epithelial cells and lung microvascular endothelial cells" pdf

Ngày tải lên : 12/08/2014, 14:20

... different of viral titers in the influenza virus infected cell supernatants between the early and late time point The quantitative cytokine and chemokine mRNA and protein expression profile of mock, influenza ... enhance the understanding of how virus infection may spread within and beyond the lung in influenza virus infection and how innate host response may contribute to modulating or aggravating tissue pathology ... routes of entry and egress from the infected cell is important in understanding the pathogenesis of influenza H5N1 infection and may help unravel the pathogenesis of human H5N1 disease Introduction...

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Báo cáo khoa học: "Tumor slices as a model to evaluate doxorubicin in vitro treatment and expression of trios of genes PRSS11, MTSS1, CLPTM1 and PRSS11, MTSS1, SMYD2 in canine mammary gland cancer" pptx

Ngày tải lên : 12/08/2014, 18:22

... counting in paraffin embedded and hematoxilin-eosin stained slides of untreated (control) and corresponding doxorubicin treated tissue specimens (Figure 1) For this examination ten circles of mm ... prognosis in canine mammary tumors [5,6], and immunohistochemical detection of Bcl2, p53 and cytokeratins, in human and canine tumors and corresponding adjacent tissues, have been similar [8] In dogs, ... upon dynamic interactions between epithelial tumor cells and their microenvironment, including stromal cells and extracellular matrix In addition, breast cancer tissue maintained in short term...

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Tài liệu Báo cáo khoa học: Trophoblast-like human choriocarcinoma cells serve as a suitable in vitro model for selective cholesteryl ester uptake from high density lipoproteins pdf

Ngày tải lên : 20/02/2014, 23:20

... maintained in medium containing 0.5 mgÆmL)1 G-418 Both cell lines were kindly provided by Dr M Krieger (MIT, Cambridge, MA, USA) Binding studies Binding studies of human HDL3 to choriocarcinoma ... inactivated fetal bovine serum (FBS, hyClone, Utah, USA) containing 100 UÆmL)1 penicillin/streptomycin and mM L-glutamine at 37 °C under 5% CO2 The JAr and Jeg3 cell lines (ATCC) were maintained ... described [31] Speciﬁc binding (4 °C) was calculated as the difference between total and nonspeciﬁc binding [37] To determine cell-associated (the sum of binding and internalization) and degraded 125I-labeled...

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Báo cáo khoa học: Transfection with 4-hydroxynonenal-metabolizing glutathione S-transferase isozymes leads to phenotypic transformation and immortalization of adherent cells pdf

Ngày tải lên : 30/03/2014, 13:20

... containing protein (cytoplasmic injections, with OG-dextran as the marker of the injected cells) or sample containing DNA (nuclear injections, with GFP as the marker of the injected cells) , using ... CCL-75 cells ranged from 40% to 70% To determine the effect of wildtype and mutant forms of GST on injected cells, all surviving HLE B-3 and CCL-75 cells were scored at 24 and 48 h and 24, 48, and ... microinjection of recombinant hGSTA1-1 and hGSTP1-1 in HLE B-3 cells (A) Typical cells 48 h after microinjection of hGSTA1-1 (p) Phase contrast micrograph showing that cells maintained their original...

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Báo cáo khoa học: "In vitro neuronal and osteogenic differentiation of mesenchymal stem cells from human umbilical cord blood" ppt

Ngày tải lên : 07/08/2014, 18:21

... llec gnimrofrep dna esu lacinilc rof SBF gnisu tuohtiw oviv xe sCSM dnapxe ot sdohtem dnif ot deen ew dna ,hcraeser ruo rof SBF desu ew ,yduts tneserp ni ,revewoH noitarefilorp sCSM rof noitidnoc ... 001-X notirT %4.0 neht dna ,nim rof enoteca %001 ,nim 01 rof lonahtem %001 dloc eci htiw detaert neht erew yehT erutarepmet moor ta nim 01 rof SBP ni edyhedlamrof %7.3 htiw dexif neht dna SBP ... noitcudni cinegoetso na desu ew ,sCSM eht morf noitaitnereffid cinegoetso ecudni oT laitnetop cinegoetso tibihxe sCSM doolb droC )A2 giF( nitnemiv dna 2HS snegitna detaler -myhcnesem rof ytivitisop...

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báo cáo khoa học: " In vitro behaviour of endothelial cells on a titanium surface" doc

Ngày tải lên : 11/08/2014, 20:20

... binding to an ECM protein (e.g fibronectin), a number of integrins mediate cellular signaling and functions It was shown that α5β1 integrin, a receptor for both fibronectin (FN) and vitronectin ... viability Indeed, the interaction of implants with host cells, and in particular with endothelial cells, might cause activation of adhesion molecules culminating with cytokine generation [2] In fact, ... TG, Klein CL, Kirkpatrick CJ: Cell-cycle control in cell-biomaterial interactions: expression of p53 and Ki67 in human umbilical vein endothelial cells in direct contact and extract testing of biomaterials...

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Báo cáo y học: "The change and effect of endothelial progenitor cells in pig with multiple organ dysfunction syndromes" ppt

Ngày tải lên : 13/08/2014, 18:22

... function of EPCs in peripheral circulation and in the bone marrow in MODS, and found that the number and function of EPCs would decrease in the progression of MODS and may be one of the main pathogenic ... function of animals and incidence of MODS All of the animals in the experimental group presented MODS (100%) and 17 out of 20 animals (85%) died in the phase of observation A total of 15 animals ... magnitude of changes was greater and the timing point of the increase was earlier (24 hours after endotoxemia) In the control group, the number of EPCs in peripheral circulation and in the bone...

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Engineering and characterization of human renal proximal tubular cells for applications in vitro toxicology and bioartifical kidneys

Ngày tải lên : 09/09/2015, 10:19

... performed by adding µl of mg/ml 4’,6’-diamidino-2’phenylindole (DAPI) and µl of mg/ml propidium iodide (PI, Invitrogen) solution to 33 each well containing ml of medium Cells were kept in the incubator ... characterization of this primary cell type obtained from different donors and sources is essential for standardized applications of this cell type in in vitro systems and for the interpretation of results ... Analysis of change in serum levels of five cytokines tested showed that the levels of granulocyte colony-stimulating factor, interleukin-6 and interleukin-10 were significantly changed in a subset of...

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Investigation of relative expression level of SLC4 bicarbonate transporter family in mouse and human corneal endothelial cells

Ngày tải lên : 13/10/2015, 15:54

... ascending limb of loop of Henle and the distal convoluted tubule (16%) and the collecting duct (4%), using various isoforms of bicarbonate transporters In the proximal tubule, exit of HCO3- from ... again independently, to obtain another batch of cells at passage and 2.5 Human corneal endothelial cells culture The HCECs were kindly provided by Dr Gary Peh Swee Lim of Ocular Tissue Engineering ... carried out using conditions reported by Kaji 24 et al and cells were cultured in minimum essential media (MEM, Invitrogen, CA, USA) with 15% fetal bovine serum (FBS, Invitrogen, CA, USA) and 20 mg/L...

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Báo cáo khoa học: Alternative initiation of transcription of the humanpresenilin 1gene in SH-SY5Y and SK-N-SH cells The role of Ets factors in the regulation ofpresenilin 1 pptx

Ngày tải lên : 16/03/2014, 18:20

... SH-SY5Y cells and similarly by about tenfold in SK-N-SH and cells Further deletion to +42 increased transcription in both cells lines: about ﬁvefold in SH-SY5Y cells and twofold in SK-N-SH cells ... members all contain the highly conserved ETS domain ERM contains two DNA binding inhibitory domains ﬂanking the Ets domain: the central 87 amino acid inhibitory domain is poorly conserved and virtually ... binding of nuclear factors present in SK-N-SH and SH-SY5Y cells to various fragments of the PS1 promoter was examined by EMSAs We have compared binding A B Fig Nuclear factors from SK-N-SH and...

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Báo cáo khoa học: In vitro gamma-secretase cleavage of the Alzheimer’s amyloid precursor protein correlates to a subset of presenilin complexes and is inhibited by zinc potx

Ngày tải lên : 16/03/2014, 23:20

... CL & Evin G (2004) Characterization of presenilin complexes from mouse and human brain using Blue Native gel electrophoresis reveals high expression in embryonic brain and minimal change in complex ... Since a functioning zinc-binding domain may be present in the Ab sequence of C99, we hypothesized that zinc may affect the oligomerization state of C101-3FLAG Therefore, the molecular mass of ... memory loss and dementia [26,27] Thus blocking Ab formation by inhibiting c-secretase is a 5551 Characterization of in vitro c-secretase activity A B D E Hoke et al C Fig Zinc inhibits in vitro c-secretase...

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báo cáo hóa học:"Interdependency of CEACAM-1, -3, -6, and -8 induced human neutrophil adhesion to endothelial cells" pdf

Ngày tải lên : 18/06/2014, 15:20

... labeling of cells Neutrophils were labeled with calcein AM (Molecular Probes, Eugene, OR) [60] by incubating × 106 cells/ ml with 50 ug of calcein AM for 30 at 37°C in 18 ml of calcein labeling ... the finding of tyrosine kinase activity, including lyn and hck, associated with CEACAM-1, CEACAM-6, and CEACAM-8, and src with CEACAM-1, suggests that these kinase activities may be involved in ... transduce signals in neutrophils resulting in activation of CD11/CD18, and an increase in neutrophil adhesion to endothelial cells, one of the critical first steps of inflammation [37] In addition,...

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báo cáo hóa học:" High correlation of the proteome patterns in bone marrow and peripheral blood blast cells in patients with acute myeloid leukemia" pot

Ngày tải lên : 18/06/2014, 15:20

... formation and maintenance of the histo-architecture Plasmonogen activator inhibitor-2 (PAI-2) Involved in the regulation and inhibition of binding between urokinase-type plasminogen activator and its ... protein for phosphoserin motifs Regulated the interactions and subcellular localization of signalling molecules γ-Catenin Forms a complex with α- and β-Catenin to Cadherins, that are involved in ... fixed in 50% ethanol, and 10% acetic acid for 12 hours Silver staining Proteins were visualized using the silver staining method employing a modification of the method of Heukeshoven according...

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Báo cáo hóa học: " In Vitro Structural and Functional Evaluation of Gold Nanoparticles Conjugated Antibiotics" doc

Ngày tải lên : 22/06/2014, 06:20

... mode of action of the antibiotics Ampicillin inhibits the cell wall biosynthesis by inhibiting the cross-linking reaction mediated by transpeptidase, while both streptomycin and kanamycin bind ... delocalization of the electron in the carbonyl group of the b-lactam ring in ampicillin at elevated temperature Elevation in temperature might induce breakage in the b-lactam ring of the free ampicillin, ... seeding of Gnps was done in presence of the antibiotics (Ampicillin, Streptomycin and Kanamycin, Fig 1) individually and thus Gnps conjugated antibiotics were formed [27] Dynamic Light Scattering...

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Báo cáo hóa học: " In Vitro Structural and Functional Evaluation of Gold Nanoparticles Conjugated Antibiotics" pdf

Ngày tải lên : 22/06/2014, 19:20

... mode of action of the antibiotics Ampicillin inhibits the cell wall biosynthesis by inhibiting the cross-linking reaction mediated by transpeptidase, while both streptomycin and kanamycin bind ... delocalization of the electron in the carbonyl group of the b-lactam ring in ampicillin at elevated temperature Elevation in temperature might induce breakage in the b-lactam ring of the free ampicillin, ... seeding of Gnps was done in presence of the antibiotics (Ampicillin, Streptomycin and Kanamycin, Fig 1) individually and thus Gnps conjugated antibiotics were formed [27] Dynamic Light Scattering...

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Báo cáo khoa học " In vitro cytostatic and immunomodulatory properties of the medicinal mushroom Lentinula edodes " pdf

Ngày tải lên : 28/06/2014, 11:20

... the in vitro comitogenic rat thymocytes test (lymphocyte transformation test, LTT) Materials and methods The strain of L edodes (Berk.) Pegler used in this study, was originated from China and ... proliferation was measured by incorporation of [3H]-thymidine expressed in counts per minute (cpm) In each of at least two experiments, means of the counts per minute (cpm) for each set of replica experiments ... (% of untreated) Fig FT-IR spectra (in KBr) of lentinan samples and the immunologically active b-glucan from Saccharomyces cerevisiae The arrows indicate absorption bands typical of lipids (a and...

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Báo cáo khoa học: " Hematology, cytochemistry and ultrastructure of blood cells in fishing cat (Felis viverrina)" ppt

Ngày tải lên : 07/08/2014, 20:23

... yltniaf dna elbinrecsidni tsomla htiw suelcun detcirtsnoc dah yehT )1 elbaT( setycokuel tnelaverp tsom eht erew slihportueN slihportueN sgniniats lacimehcotyc lla rof evitagen erew yehT dnuof netfo ... tiknurihirP kasgnaerK 461 .ylevitcepser ,SAP dna ulg-β rof evitisop ylkaew )G & F( EANA rof evitisop ylgnorts )E( ylevitcepser ,BBS dna REP rof evitagen )D & C( ylevitcepser ,W dna GW ,selunarg ... ,selunarg epahs-dor htiw lihponisoe na )B & A( :slihponisoE SAP rof evitisop ylgnorts )G( ulg-β rof deniats ylevitisop )F( EANA rof evitisop ylkaew )E( ylevitcepser ,BBS dna REP ,slihportuen evitisop...

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Báo cáo y học: "Induction of HSPA4 and HSPA14 by NBS1 overexpression contributes to NBS1-induced in vitro metastatic and transformation activity" potx

Ngày tải lên : 10/08/2014, 05:21

... overexpression in H1299 cells increased in vitro migration and invasion activity and induced the expression of HSPA4 and HSPA14 We previously demonstrated that NBS1 overexpression induces epithelial-mesenchymal ... knockdown of HSPA4 and HSPA14 did not further decrease the in vitro migration and invasion activity in H1299 cells (A) RT-PCR analysis of H1299 cells with knockdown of HSPA4, HSPA14, or both (B) The in ... results from microarray screening, a RTPCR assay was performed to examine the increase in mRNA levels of HSPA4 and HSPA14 The result showed that the expression levels of HSPA4 and HSPA14 increased in...

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báo cáo khoa học: " Characterization and isolation of a T-DNA tagged banana promoter active during in vitro culture and low temperature stress" ppt

Ngày tải lên : 12/08/2014, 03:20

... were recorded with an integration time of 20 Time lapses were taken at an interval of 30 for screenings on ZZ and RD2 medium, and 20 for screenings of in vitro plantlets maintained on REG medium ... RT-PCR on samples of proliferating material initiated from in vitro apical meristems of line 17 was consistent with these results (data not shown) indicating that sequences 17-1 and 17-2 are also ... active in proliferating meristem cultures We also investigated transcription of all 3' tagged sequences of line 17 by RT-PCR in cell colonies (stage I) as well as leaf and root tissues of in vitro...

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