in vitro characterization of ls replacements
Báo cáo khoa học: In vitro characterization of a plastid terminal oxidase (PTOX) pot
Ngày tải lên :
31/03/2014, 07:20
... conserved Cys in the N-terminal domain of PTOX [3] whilst a conserved Cys in AOX is involved in the stimulation of AOX activity by a-keto acids [13,14,22] When examining the effect of pyruvate on PTOX ... of the given inhibitors Activity before addition of inhibitor is set at 100 (absolute amount were calculated as explained in Table 1) Inhibitors of PTOX activity in vitro It is worth mentioning ... quinone binding site would be expected to be close to iron binding amino acids, which is the case here ([6], Fig 4) This potential quinone-binding site appears to be conserved in PTOX sequences (including...
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Báo cáo khoa học: "In vitro characterization of cells derived from chordoma cell line U-CH1 following treatment with X-rays, heavy ions and chemotherapeutic drugs" pdf
Ngày tải lên :
09/08/2014, 09:21
... treatment, cycling cells in T12.5 culture flasks were exposed to series of concentration of bleocin, a single component of bleomycin family group A (Calbiochem, Japan), which induces DNA strand ... lines retain a wild-type allele of p53 gene, although our sequence result exhibited a heterozygous mutation C > G, causing an amino acid substitution of proline 72 to arginine (Figure 1) Since the ... bleocin, B) cisplatin, C) mitomycin C, and D) camptothecin Black square represents HeLa cells, white triangle indicates U87-MG cells, and black reverse triangle indicates U-CH1-N chordoma cell line...
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báo cáo hóa học: " Beyond platinum: synthesis, characterization, and in vitro toxicity of Cu(II)-releasing polymer nanoparticles for potential use as a drug delivery vector" ppt
Ngày tải lên :
21/06/2014, 02:20
... containing other metal/ligand combinations that can be envisaged [37] Here, we report the synthesis, characterization, and metal binding properties of our Cu-binding particles, as well as preliminary ... contained in the original loading solution determined Cu loading, resulting in values ranging between 12 and 16 wt.% based on these reactions conditions XPS was used to further confirm Cu loading ... Cu release (see Discussion) In vitro toxicity of CuCNPs in HeLa cells The in vitro toxicity of CuCNPs in HeLa cells (a cervical adenocarcinoma) was investigated via an assay based on the MTT reagent...
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Analysis, sequencing and in vitro expression of PCR products
Ngày tải lên :
25/10/2013, 22:20
... but also protein fragments for mapping of interaction domains The use of in vitro expression approaches to analyze protein products is likely to gain momentum for the analysis of known proteins ... functionally analyzed using in vitro assays Furthermore, rapid in vitro protein–protein interaction studies could be performed using this technique where not only full-length version of proteins could be ... strategies The in vitro expression of proteins can also be used for functional studies of proteins For example, enzymes synthesized in vitro are likely to be correctly folded and to retain enzymatic...
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Báo cáo khoa học: In vitro expansion of DNA triplet repeats with bulge binders and different DNA polymerases pdf
Ngày tải lên :
07/03/2014, 06:20
... reaction by using 32P-labeling primer or template in the presence or absence of DNA-binding agents (DDIs and doxorubicin) The DNA bulge binding of both compounds was detected by CD and UV melting experiments ... is formed To gain insight into the binding of drugs to bulge DNA, several bulgecontaining oligonucleotides were selected as binding hosts for DDI-1A and DDI-1B CD spectroscopy of DDI-1A (Fig ... two-base bulge in DNA via stacking of its helically oriented aromatic ring systems on the bulge-flanking base pairs that define the long sides of the triangular prism binding pocket, with its amino sugar...
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Báo cáo khoa học: The in vitro effects of CRE-decoy oligonucleotides in combination with conventional chemotherapy in colorectal cancer cell lines potx
Ngày tải lên :
07/03/2014, 15:20
... was investigated in GEO cells only, as inhibition of cell proliferation was not seen in the HCT116 cells At the end of the treatment schedules, the surviving fractions of GEO cells were plated in ... alone did not increase the extent of staining However, in cells that had been cocultured with CDO and a cytotoxic drug, staining was significantly increased, indicating the presence of senescence ... was increased in GEO but unchanged in HCT116 cells (B) Standard immunoblots for cyclin and CDK levels in GEO cells (C) Immunoprecipitation experiments highlighting cyclin/CDK interactions in the...
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In Vitro Screening of Plant Resources for Extra-Nutritional Attributes in Ruminants: Nuclear and Related Methodologies pptx
Ngày tải lên :
15/03/2014, 04:20
... challenges of extrapolating in vitro findings to in vivo evaluation of plant resources The chapters in this book are written by experts interested in exploring and making better use of plant biodiversity ... the initial screening is conducted in vitro because of the large number of candidate plant species and the prohibitive cost of screening them in vivo The number of species for in vivo testing ... all ground in the same way A range of grinder types can be used for grinding plant samples including hammer mills; Wiley mills and cross-beater mills are all suitable machines, providing they have...
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Báo cáo khoa học: Bridging the gap between in silico and cell-based analysis of the nuclear factor-jB signaling pathway by in vitro studies of IKK2 ppt
Ngày tải lên :
16/03/2014, 11:20
... effects of future inhibitors designed for this NF-jB signaling pathway should be tested not just in vitro and cell based but also simulated in silico Combining this method of analysis (in vitro, ... Stimulation of cells was performed at 10-min intervals for 400 with IL-1a After 400 min, the plates were inverted to remove media into a dish containing Virkon disinfectant to destroy cells not adhered ... = Intensity of nucleus Intensity of cytoplasm Intensity of nucleus Scheme This is a simplication of a cell as seen by analysis software, where the software measures the intensity of NF-jB in...
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Báo cáo khoa học: In vitro analysis of the relationship between endonuclease and maturase activities in the bi-functional doc
Ngày tải lên :
17/03/2014, 10:20
... stringent concentration of KCl, there was a correlation between inhibition of endonuclease activity and inhibition of splicing activity indicating that RNA binding can be monitored by measuring ... and its binding affinity is not altered by the presence of DNA in the DNA binding site Interestingly, when nM RNA and 10 nM DNA were added simultaneously to a limiting amount of protein in TK8 buffer ... background In vitro competition assays RNA splicing and DNA cleavage competition experiments both involved a prebinding step in which either DNA or RNA substrates were incubated with I-AniI in a binding...
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Báo cáo khoa học: Chick embryo anchored alkaline phosphatase and mineralization process in vitro Influence of Ca 2+ and nature of substrates doc
Ngày tải lên :
23/03/2014, 17:21
... in the GPI-bALP mediated mineralization: effects of Ca2+ concentration To assess the role of Ca2+ in initiating the mineralization process, we modelled the biological process by using 0.8 U of ... Ca2+ was added in the incubation medium, no calcium-phosphate minerals were obtained during 72 h of incubation The Ca/Pi ratio was 0.8 at 144 h, characteristic of a poorly crystalline structure ... presence of anchored GPI-bALP (Fig 1) The presence of HA in the mineral samples was confirmed by In uence of other phosphate substrates in the mineralization process To examine the effects of substrates...
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in vitro release of ketoprofen from proprietary and extemporaneously manufactured gels
Ngày tải lên :
13/04/2014, 09:25
... events include extrusion of lamellar bodies, loss of nucleus and increasing amount of keratin in the stratum corneum (12) The origins of the cells of the epidermis lie in the basal lamina between ... Effect of molarity and pH on the diffusion profile of ketoprofen .85 Effect of temperature on the diffusion profile of ketoprofen 86 Effect of mass on the diffusion profile of ketoprofen ... activity of a penetrant in a formulation resulting in changes in partitioning tendencies (18) 1.2.4 Barrier function of the skin The natural function of the skin is the protection of the body against...
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báo cáo hóa học:" In vitro generation of cytotoxic and regulatory T cells by fusions of human dendritic cells and hepatocellular carcinoma cells" docx
Ngày tải lên :
18/06/2014, 15:20
... fusion cells producing TGF-β resulted in the induction of Treg in vivo and in vitro in a murine model [52] Moreover, the blockade of TGF-β reduces Treg induction by the fusion cell vaccine and ... DCs/auto-HCC in vitro, the expression of IFN-γ in both CD4+ and CD8+ T cells were much lower (Figure 6D) In contrast, the expression of IFN-γ in CD4+ and CD8+ T cells significantly increased after vaccination ... OK432 is administrated, resulting the generation of Treg in vitro The lack of therapeutic efficacy with fusion cell vaccine in the patient may be not due to low levels of CTL response but inhibitory...
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Báo cáo sinh học: " In vitro permissivity of bovine cells for wild-type and vaccinal myxoma virus strain" pot
Ngày tải lên :
18/06/2014, 18:20
... http://www.virologyj.com/content/4/1/94 information concerning interactions between MYXV and bovine cells is available yet In this study, we characterized the infection of bovine cell lines and bovine peripheral blood ... KOP-R P3 BT MDBK Figure Permissivity of bovine cell lines for myxoma virus Permissivity of bovine cell lines for myxoma virus Bovine cells were maintained in DMEM (KOP-R and BT) or MEM (MDBK) ... of viral maturation are impaired in BT cells A B To evaluate MYXV infection in blood primary bovine cells, peripheral blood mononuclear cells (PBMC) were infected Bovine blood was collected in...
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Báo cáo sinh học: "In vitro migration of cytotoxic T lymphocyte derived from a colon carcinoma patient is dependent on CCL2 and CCR2" pot
Ngày tải lên :
18/06/2014, 19:20
... after adding T cells), fixed in buffered formalin and embedded in paraffin a, b: Staining with H&E c, d: Specific brown staining of apoptotic cells by anti-caspase Ab e, f: In situ end-labeling (ISEL); ... percentage of apoptotic tumor cells was determined by counting apoptotic nuclei and intact tumor cells in sections stained with H&E Blocking of T-cell migration in reconstruct T-cell migration in the ... cells resulting in tumor growth inhibition (reviewed in [25] and [1]) In mouse systems, ex vivo transduction of chemokines into tumor cells has provided potent tumor vaccines inducing tumor rejection,...
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báo cáo hóa học: "Bayesian aggregation versus majority vote in the characterization of non-specific arm pain based on quantitative needle electromyography" pot
Ngày tải lên :
19/06/2014, 08:20
... of two conditional probabilities: that of being detected in a muscle of a subject with NSAP and that of being detected in a muscle of a subject free of pain If we maintain our understanding of ... and obtain an estimate of the surface representation of the MUP (called an SMUP template) related to each MUPT The SMUP is determined by using the firing times of the main spike of each individual ... examination of the upper extremities, including myotome testing, dermatome (light touch, pin prick) testing, and assessment of the deep tendon reflexes at the C5 to C8 levels Cervical spine range...
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Báo cáo hóa học: " In vitro permissivity of bovine cells for wild-type and vaccinal myxoma virus strains" potx
Ngày tải lên :
20/06/2014, 01:20
... http://www.virologyj.com/content/4/1/94 information concerning interactions between MYXV and bovine cells is available yet In this study, we characterized the infection of bovine cell lines and bovine peripheral blood ... KOP-R P3 BT MDBK Figure Permissivity of bovine cell lines for myxoma virus Permissivity of bovine cell lines for myxoma virus Bovine cells were maintained in DMEM (KOP-R and BT) or MEM (MDBK) ... of viral maturation are impaired in BT cells A B To evaluate MYXV infection in blood primary bovine cells, peripheral blood mononuclear cells (PBMC) were infected Bovine blood was collected in...
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Báo cáo hóa học: " In vitro effects of selenium deficiency on West Nile virus replication and cytopathogenicity" ppt
Ngày tải lên :
20/06/2014, 01:20
... comprised of insulin, transferrin and bovine serum albumin as a substitute for FBS, whereas in studies employing Huh cells, Se deficiency was induced by growing cells with media containing 0.01% ... loss of GPx-1 protein at all time points in both cell types (Fig 2C) Addition of sodium selenite significantly induced GPx1 protein levels in Vero cells but not in SK-NSH cells, thus supporting ... apoptosis in WNV-infected Vero cells Vero cells were inoculated with WNV NY99 strain at multiplicity of infection (MOI) 1, at day post-induction of Se deficiency, to study the kinetics of virus...
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Báo cáo hóa học: " In vitro inhibition of human influenza A virus replication by chloroquine" pot
Ngày tải lên :
20/06/2014, 01:20
... testing the susceptibility of a wide range of clinical isolates of influenza A viruses to chloroquine, as well as in vivo studies, would be needed to determine the role, if any, of chloroquine in ... pathology of avian influenza infection in humans appears to be mediated by pro-inflammatory cytokines [13] Although we have shown that chloroquine is able to inhibit two reference subtypes of influenza ... chloroquine in the prophylaxis and treatment against influenza infection It would also be interesting to determine the activity of chloroquine against the highly pathogenic H5N1 subtype as this...
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báo cáo hóa học:" In vitro migration of cytotoxic T lymphocyte derived from a colon carcinoma patient is dependent on CCL2 and CCR2" pptx
Ngày tải lên :
20/06/2014, 03:20
... after adding T cells), fixed in buffered formalin and embedded in paraffin a, b: Staining with H&E c, d: Specific brown staining of apoptotic cells by anti-caspase Ab e, f: In situ end-labeling (ISEL); ... percentage of apoptotic tumor cells was determined by counting apoptotic nuclei and intact tumor cells in sections stained with H&E Blocking of T-cell migration in reconstruct T-cell migration in the ... cells resulting in tumor growth inhibition (reviewed in [25] and [1]) In mouse systems, ex vivo transduction of chemokines into tumor cells has provided potent tumor vaccines inducing tumor rejection,...
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báo cáo hóa học:" In vitro suppression of the MMP-3 gene in normal and cytokine-treated human chondrosarcoma using small interfering RNA" doc
Ngày tải lên :
20/06/2014, 04:20
... After of incubation with 0.4% Trypan blue, the percentages of stained cells (indicative of nonviable cells) versus stain-excluding cells were counted in a hemocytometer Then the percentage of viable ... number of apoptosis cells × 100)/total number of cells Morphology of the cells was studied by using aceto-orcein dyes that can separate mitotic cells from interphase cells Then the percentage of ... Belgium) to remove traces of FCS After removing HBSS, the cells were trypsinized with ml of trypsinEDTA (BioWhittaker™ Cambrex Bio Science) After examining the cells using an inverted microscope to...
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