in vitro and animal studies
Báo cáo khoa học: Insertion of the plant photosystem I subunit G into the thylakoid membrane In vitro and in vivo studies of wild-type and tagged versions of the protein doc
... promoter and terminator, and into vectors for in vitro transcription and translation An Arabidopsis line (psag-1.4 [5], termed DG in this report), lacking PSI-G due to a transposon footprint in exon ... shown) In conclusion, we have shown using both in vitro and in vivo methods, that PSI-G adopts a topology in the thylakoid membrane with the loop facing the stroma and its N- and C-termini facing ... after 0, 30 and 60 digestion with trypsin, probed with antibodies directed against PSI-G, PSI-K (intrinsic membrane protein, stromal loop), PSI-O (intrinsic membrane protein, luminal loop) and PSI-D...
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Báo cáo khoa học: Structural studies of the capsular polysaccharide and lipopolysaccharide O-antigen of Aeromonas salmonicida strain 80204-1 produced under in vitro and in vivo growth conditions docx
... CPS and O-chain LPS are composed of linear trisaccharide repeating units containing 3-linked 2-acetamido-2-deoxy-D-quinovose, 4-linked 3-[(N-acetyl-L-alanyl) amido]-3-deoxy-D-quinovose and 3-linked ... Interresidue NOEs were observed between H-1a and H-3c and between H-1b and H-3a, suggesting a linear structure a-c-b (Fig 2E,F) In addition, interresidue NOEs between H-1c and H-4b (Fig 2G) indicated ... and O-chain polysaccharide of A salmonicida strain 80204-1 the previously identified O-chain containing L-rhamnose, 2-amino-2-deoxy-D-mannose and D-glucose [11], confirming that the novel CPS and...
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báo cáo hóa học:" Functional significance of the signal transduction pathways Akt and Erk in ovarian follicles: in vitro and in vivo studies in cattle and sheep" ppt
... in mediating the effects of FSH on inhibin-A, activin-A, oestradiol and progesterone secretion and also important in mediating IGF-I stimulated inhibin-A, activin-A, follistatin, oestradiol and ... previously in the bovine model Increases in Akt and Erk signalling proteins in response to FSH and IGF stimulation suggest a role for Akt and Erk signal transduction pathways in FSH and IGF mediated ... for luteinizing hormone and its interaction with insulin: activation of Janus kinase/signal transducer and activator of transcription and phosphoinositol 3-kinase/Akt pathways Endocrinology 2003,...
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Probiotic Lactobacillus Strains: in vitro and in vivo Studies pot
... immunomodulating properties in in vivo mouse model MATERIAL AND METHODS Survival of Lactobacillus strains at low pH and in the presence of bile salt Lactobacillus strains isolated from feces of healthy infants ... Serum and Vaccine Producer Biomed, Cracow, Poland), Salmonella enterica subsp enterica sv Enteritidis (clinical isolate obtained from the Institute of Sea and Tropical Medicine, Gdynia, Poland), ... KALLIOMAKI M., KIRJAVAINEN P., EEROLA E., KERO P., SALMINEN S., ISOLAURI E.: Distinct patterns of neonatal gut microflora in infants in whom atopy was and was not developing, J.Allergy Clin.Immunol 107,...
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In vitro and in vivo studies into the antidiabetic and antilipidemic effects of chlorogenic acid
... reduction in glucose and insulin levels 15mins after oral glucose load in an OGTT [111] However, no effect was observed on fasting glucose and insulin levels, contradicting the previous finding in normaglycemic ... caffeine, and (iv) CGA possesses hypoglycemic effects in human, animal and in vitro models, the present author's next aim is to investigate the hypoglycemic effects of CGA in both in vivo and in vitro ... Activation of insulin signaling upon binding of insulin to insulin receptors causes suppression of gluconeogenesis in liver and kidney [14], translocation of glucose transporter-4 (GLUT 4) from inner...
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Tài liệu Báo cáo khoa học: The endogenous retinoid metabolite S-4-oxo-9-cis-13,14-dihydro-retinoic acid activates retinoic acid receptor signalling both in vitro and in vivo pdf
... ⁄ v) fetal bovine serum (Gibco Invitrogen), 1% (v ⁄ v) PEST (Gibco Invitrogen), 1% (v ⁄ v) l-glutamine (Gibco Invitrogen) and 1% nonessential amino acids (Gibco Invitrogen) Murine hepatoma-1 ... grown in low-glucose DMEM (Gibco Invitrogen) supplemented with 10% (v ⁄ v) fetal bovine serum (Gibco Invitrogen), 1% (v ⁄ v) PEST (Gibco Invitrogen), 1% (v ⁄ v) l-glutamine (Gibco Invitrogen) and ... all-trans-retinoic acid, the findings clearly demonstrate that S-4o9cDH-RA has the capacity to bind and activate nuclear retinoid receptors and regulate gene transcription both in vitro and in vivo Retinoids...
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Báo cáo khoa học: Two types of replication protein A in seed plants Characterization of their functions in vitro and in vivo ppt
... USA) and transformed into E coli for protein induction Extracts prepared from cells induced for h were shown to contain a six-histidine C-terminal-tagged OsRPA14 fusion protein The OsRPA14 protein ... tag were obtained by in vitro translation in wheatgerm extract (Invitrotec) according to the manufacturer’s procedure OsRPA70a (or OsRPA70b) and OsRPA32, or OsRPA32 and OsRPA14 were incubated with ... soluble in the presence of m urea Soluble fractions were loaded on to columns containing 10 mL His-Bind resin (Novagen) The columns were washed with 50 mL binding buffer containing m urea and then...
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Báo cáo khoa học: In vitro and in vivo self-cleavage of Streptococcus pneumoniae signal peptidase I pot
... the protein The self-cleavage of LexA protein is important in the SOS response in bacteria [21–23] In vitro self-cleavage was also observed in all investigated bacterial SPase I including enzymes ... we not know if the self-cleavage is activating or inactivating the enzyme, and if the selfcleavage is happening in vivo within the bacterial cells In the current study, we have shown that phospholipid ... pneumoniae cells in all the growth phases, and the cells maintain the active SPase I at the highest level in the exponential growth phase MATERIALS AND METHODS Materials and bacterial strains Restriction...
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Báo cáo khóa học: Determination of modulation of ligand properties of synthetic complex-type biantennary N-glycans by introduction of bisecting GlcNAc in silico, in vitro and in vivo pot
... nonspecific binding by protein–protein interactions, cells were incubated with 100 lg ligand-free carrier protein (BSA) per mL for 30 at °C before incubation with the biotinylated neoglycoprotein in Dulbecco’s ... avoided In the solid-phase assay, saturable and carbohydrate-dependent binding was measured, and the resulting Scatchard plots gave straight lines indicating the presence of a single class of binding ... neoglycoproteins, we determined their binding to cells and to tissue sections as well as their biodistribution in mice after injection of radioiodinated material Binding to cells and organs In these...
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Báo cáo khoa học: Searching for folded proteins in vitro and in silico docx
... protein domains arose is thus not clear These difficulties pose interesting questions relating to the understanding of both protein evolution and protein engineering From an evolutionary standpoint, ... bind its natural ligand For the host protein to fold it must be able to bring the N- and C-termini of the loop together Thus, folding of the insert protein in such a way as to bring its N- and ... library containing only glutamine (Q), leucine (L) and arginine (R) They estimated that > 5% of the proteins were expressible in Escherichia coli and 1–2% showed cooperative unfolding (a characteristic...
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Báo cáo hóa học: " Vaccinia virus lacking the deoxyuridine triphosphatase gene (F2L) replicates well in vitro and in vivo, but is hypersensitive to the antiviral drug " pot
... differences in replication in mice reflect real differences in the biology of these viruses, or are related to differences in the animal models including route of infection In HSV, direct intracranial inoculation ... repeatable increase in the efficacy of N-methanocarbathymidine (N-MCT) (Table 2) This compound is a carbocyclic thymidine analog that inhibits the replication of VV both in vitro and in vivo [12,13], and ... evaluated in cell culture and in intranasally infected mice The deletion of F2L had a minimal impact on viral replication in vitro and did not appear to significantly reduce the virulence in vivo...
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Báo cáo hóa học: " N-methylisatin-beta-thiosemicarbazone derivative (SCH 16) is an inhibitor of Japanese encephalitis virus infection in vitro and in vivo" ppt
... reports describing the in vitro inhibitory effect of compounds such as ribavirin, mycophenolic acid, imino sugars, inhibitors of serine protease, RNA interference and non-steroidal anti-inflammatory ... lanes and – in vitro translation products of RNA obtained form JEV infected PS cells (untreated) at and 10 hours post infection respectively Lanes and – in vitro translation product of RNA obtained ... Comparative study of the effects of isatin, an endogenous MAO-inhibitor, and seleginine on bradykinasia and dopamine levels in a rat model Parkinson's disease induced by the Japanese encephalitis...
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Báo cáo hóa học: " Inhibition of foot-and-mouth disease virus replication in vitro and in vivo by small interfering RNA" pot
... J, Ince N, Ouyang N, Min J, Chen J, Shankar P, Lieberman J: RNA interference targeting Fas protects mice from fulminant hepatitis Nat Med 2003, 9:347-351 Gitlin L, Karelsky S, Andino R: Short interfering ... growing in a monolayer, and having a well-defined tendency for parallel orientation Viral infection causes a marked CPE resulting in total cellular detachment, rounding, and destruction, which ... 36 and 48 h postinfection Samples of supernatant were obtained at designated time points, and the TCID50 were determined by the Reed-Muench formula BHK-21 cells are fibroblastic, growing in a...
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báo cáo hóa học: " In vitro and in vivo pre-clinical analysis of a F(ab’)2 fragment of panitumumab for molecular imaging and therapy of HER1-positive cancers" pot
... following i.p injection of the panitumumab F(ab’)2 Imaging studies, g-scinitigraphy and PET, of the i.p tumor model using these two injection routes are pending Studies from this laboratory and ... radiolabelings TN performed PET imaging and analysis MWB synthesized the chelate and participated in the design of the studies DEM conceived of and coordinated the studies and assisted with the in vivo ... distribution in these last two studies was similar to what was obtained with the i.v injected 11 1In- labeled panitumumab F(ab’)2 already discussed Discussion The in vivo and in vitro properties of the intact...
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Báo cáo hóa học: " CdTe quantum dots with daunorubicin induce apoptosis of multidrug-resistant human hepatoma HepG2/ADM cells: in vitro and in vivo evaluation" pptx
... cultured in the cell culture medium containing μg/ mL adriamycin (Sigma) Both cell lines were maintained in RPMI-1640 medium containing 10% FCS, 100 U/ml of penicillin, and 100 μg/ml of streptomycin ... and fixed in 100% methanol for 10 Cell monolayers were blocked in 5% BSA in PBS for 45 and incubated for h at room temperature with P-gp antibodies (Invitrogen, Beijing, China), followed by incubation ... were suspended in 100 μL of culture medium and subcutaneously inoculated into the right flank of mice using a 1.0 mL syringe Intravenous injection of reagents and tumor growth inhibition study...
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Báo cáo sinh học: " CNS progenitor cells and oligodendrocytes are targets of chemotherapeutic agents in vitro and in vivo" pdf
... TUNEL staining, doublecortin staining, S-100b staining, and the merged image (a-d) Images taken at -4 µm; (e-h) -2 µm; (i-l) µm; (m-p) µm The congruence between the doublecortin+ staining and the ... provide rapid in vitro screens for analyzing new therapies and discovering means of achieving selective protection or targeted killing In light of the ease of use of these in vitro and in vivo assays, ... with cytarabine leads to increased and prolonged cell death, and decreased BrdU incorporation, in the adult mouse CNS Cell death and BrdU incorporation were examined as in Figures and (a) The...
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Báo cáo khoa học: "In vitro and in vivo gene therapy with CMV vector-mediated presumed dog b-nerve growth factor in pyridoxine-induced neuropathy dogs" pot
... of pyridoxine injection On the fourth day of pyridoxine injection, all dogs held their hindlimb stiffly when standing These conditions were maintained until the end of the pyridoxine injection ... determine the effect of the cytomegalovirus (CMV) vector-mediated gene transfer of the pdβ-NGF in vitro and gene therapy using recombinant pdβ-NGF plasmid in the dog model, having pyridoxine-induced ... lesions in the lateral, dorsal or ventral funiculus, or in the gray matter of L4 in the negative control group (Fig 3A) The axons and myelin was Presumed dog β-NGF gene therapy in vitro and in vivo...
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Báo cáo khoa học: "Effects of sustained subculture on apparent rejuvenation of the apple rootstock M.9 in vitro and in vivo" pps
... expressed as improved shoot production and rooting in vitro and subsequently of micropropagated plants in the field Similar increased rooting ability of cuttings from micropropagated stockplants ... sustained for at least yr (Howard et aL, 1989a) Differences in shoot production and rooting of M.9 in vitro also appeared to originate from differences between explants used to initiate culture lines ... proliferation medium improved subsequent rooting at the 11th and l5th subcultures but not at the 21 st subculture plantlets in compost following in vitro rooting but was increased significantly (P
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- sustainable practices in agriculture and animal husbandry
- confocal microscopy of skin in vitro and ex vivo
- persistence in environment and animal products
- techniques for assessment of interactions of mucins with microbes and parasites in vitro and in vivo
- mutations their transition rates and involvements in human and animal disorders
- interpretation of in vitro skin absorption studies of lipophilic chemicals
- in vitro and cosmophysical interfaces in short term geomagnetic deprivation
- hairy roots in phytoremediation and phytomining studies
- assays to assess effectiveness in vitro and in vivo
- mononucleosomes dinucleosomes and nucleosome arrays in vitro and analysis of transcription factor bind
- in vitro and in vivo assays
- in vitro and in vivo analysis of the cell
- in vitro and in vivo osteogenesis
- in vitro and in vivo applications
- value in human and animal diets
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