in situ x ray tensile stretching apparatus

Tài liệu Báo cáo khoa học: Destabilization of psychrotrophic RNase HI in a localized fashion as revealed by mutational and X-ray crystallographic analyses pdf

Ngày tải lên : 18/02/2014, 13:20

... of the intrinsic ﬂexibility of the loop containing His124 and the crystal packing effect Destabilization mechanism of So-RNase HI A combination of the six thermostabilizing mutations increases ... denaturation induced by single amino acid substitution in the sequence of beta-glucosidase A from Bacillus polymyxa Proteins 33, 567–576 Bae E & Phillips GN Jr (2005) Identifying and engineering ion ... So-RNase HI by 28.8 °C in Tm and 27.0 kJÆmol)1 in DG(H2O) Five of the six substituted residues in the resultant sextuple mutant protein (6·-RNase HI) are found in the corresponding positions of at...

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Tài liệu Báo cáo khoa học: Molecular determinants of ligand speciﬁcity in family 11 carbohydrate binding modules – an NMR, X-ray crystallography and computational chemistry approach doc

Ngày tải lên : 18/02/2014, 17:20

... (Fig 1III–V), which could indicate that the corresponding hydroxyl groups are involved in protein binding The results for the linebroadening measurements of protons H1a in the alpha and beta configurations, ... been grouped into three subfamilies: ‘surface-binding’ CBMs (type A), ‘glycan-chain-binding’ CBMs (type B), and ‘small sugar-binding’ CBMs (type C) [5] Determinants of ligand specificity in CtCBM11 ... over other methods in detecting binding activity First, the binding component can usually be directly identified, even from a substance mixture, allowing it to be utilized in screening for ligands...

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Tài liệu Báo cáo khoa học: NMR structure of AII in solution compared with the X-ray structure of AII bound to the mAb Fab131 pptx

Ngày tải lên : 20/02/2014, 23:20

... Asp1 side chain carboxylic group was suggested to be involved in hydrogen-bonding interaction with either the N-terminal amino group or the Arg2 side chain amino group, whereas in our studies ... Mixing times for NOESY experiments were set to 100, 200, 350 and 400 ms to determine NOE build up rates A mixing time of 350 ms provided sufﬁcient cross-peak intensity without introducing spin-diffusion ... chain NH of the preceding arginine, forming a stable heptamer ring, both in the X- ray structure and the solution structure of AII (Fig 8) Indeed, aspartate residues at the N-termini of short polypeptides...

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Báo cáo khoa học: The effect of replacing the axial methionine ligand with a lysine residue in cytochrome c-550 from Paracoccus versutus assessed by X-ray crystallography and unfolding ppt

Ngày tải lên : 07/03/2014, 17:20

... basic f-amino group of the coordinating K100, with the H-bonded water molecule found in the X- ray structure increasing the electron donating power of the coordinating amino group which in turn ... prominent being the absence of the 695-nm band, indicative of Met-Sdiron coordination Upon titrating increasing amounts of guanidinium hydrochloride (GdmHCl) the protein unfolds resulting in the ... K100 was coordinating the heme-iron [7] Interestingly, ligand exchange for the M100K variant at alkaline pH was not observed [7], suggesting that the ‘alkaline transition’ involving the dissociation...

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Báo cáo Y học: The role of zinc in the methylation of the coenzyme M thiol group in methanol:coenzyme M methyltransferase from Methanosarcina barkeri New insights from X-ray absorption spectroscopy doc

Ngày tải lên : 17/03/2014, 23:20

... putative HXCXnC zinc binding motif [15] The latter nding indicates that zinc coordination in MtaA and MtbA should be the same The nding that the number of sulfur ligands to zinc can vary in different ... totally excluded) MtbA is inactivated when Cys241 or Cys316 of the putative zinc binding motif H239XC241XnC316 are mutated [23] In case of MtaA the mutation of Cys239 in the H237XC239XnC316 motif ... addition indicates that also in His237 Ala the HS-CoM binding results in sulfur ligation to the active-site zinc XANES spectra of wild-type and mutants As shown in the following, the interpretation...

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Báo cáo khoa học: X-ray structure of glucose/galactose receptor from Salmonella typhimurium in complex with the physiological ligand, (2R)-glyceryl-b-D-galactopyranoside pdf

Ngày tải lên : 23/03/2014, 04:21

... of glucose-binding protein has been identiﬁed in some bacteria; its fold is not similar to GBP, but rather to that of the larger maltose-binding protein This kind of protein is exempliﬁed by ... observed in the cleft between the two domains (Fig 1) As illustrated in Fig 2, 15 hydrogen bonds directly link protein and ligand, six of which arise from domain 1, and nine from domain Two water ... moiety (marked with red ovals in Fig 2B): B Fig Interactions in the binding site (A) Stereoview of bound GGal showing GBP residues making hydrogen-bonding and aromatic interactions (B) Schematic...

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Báo cáo khoa học: Structural ﬂexibility in Trypanosoma brucei enolase revealed by X-ray crystallography and molecular dynamics pdf

Ngày tải lên : 23/03/2014, 07:20

... site involving a His residue at position ‘0’ (i.e immediately preceding the natural initiator Met within the tail downstream of the thrombin cleavage site in the N-terminal extension containing ... novel metal binding site Furthermore, they show structural heterogeneity in their inhibitor-bound forms, highlighting the potential to extend future inhibitors out of the ligand-binding pocket ... structures (Fig 3) to determine to what extent these modes of structural variability are explored The PEP complex simulation remains in the vicinity of the starting point There is little tendency...

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Báo cáo khoa học: Protein interactions in the sumoylation cascade – lessons from X-ray structures pdf

Ngày tải lên : 30/03/2014, 04:20

... Ala72 in Nedd8, and Arg72 in ubiquitin (highlighted in yellow in Fig 2C) Matching E1 residues are Thr149 in uba2, Arg190 in uba3 and Gln608 in uba1 (highlighted in purple in Fig 3E) Interestingly, ... domain [1,42,44,45] Except for the SP-RING domain, no homology has been found between PIAS proteins and single-chain ubiquitin E3 ligases NSE2a and TOPORS, two other proteins that contain RING ... SP-RING domain, a SUMO interaction motif (SIM) and a C-terminal domain (CTD) [39] (B) Pc2 contains an uncharacterized E3 domain, a ubc9 binding domain and a Pro-Ile-Asp-Leu-Ser motif involved in substrate...

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the restless universe understanding x-ray astronomy in the age of chandra and newton oct 2002

Ngày tải lên : 11/06/2014, 10:39

... an X ray is Unfortunately, the term X ray, so applied, refers to the piece of film illuminated by X rays Mention X- ray astronomy” to people and too often they think we send beams of X rays into ... s, X rays were believed to exist only in the laboratory because of the difficulty of creating them Laboratory experiments established that air absorbs X rays, explaining the lack of X- ray detections ... the X- rays used in dentists’ and doctors’ offices There, the X rays have interacted with light-sensitive grains on photographic film.10 The absorption of the X rays by the grains causes the grains...

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báo cáo hóa học:" Accuracy of biplane x-ray imaging combined with model-based tracking for measuring in-vivo patellofemoral joint motion" doc

Ngày tải lên : 20/06/2014, 01:20

... biplane x- ray system [36] Testing procedures Biplane x- ray images were acquired while manually flexing the knee from full extension (i.e., approximately 10° flexion) to approximately 90° of flexion ... the biplane x- ray system's 3D imaging volume, i.e., the region defined by the intersecting x- ray beams Although this limitation prevents us from collecting biplane x- ray images during an entire ... techniques for assessing in- vivo PF joint motion have included dynamic CT imaging [34] and single-plane fluoroscopic imaging combined with shape matching [35] Dynamic CT imaging has limitations...

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Pore Characterization in Low-k Dielectric Films Using X-ray Reflectivity: X-ray Porosimetry pot

Ngày tải lên : 27/06/2014, 10:20

... chamber is in a vacuum-tight aluminum housing, sealed with a Viton o-ring, and has X- ray transparent beryllium windows to allow the X- rays to enter and exit 25 ◆ Pore Characterization: X- ray Porosimetry ... the X- rays to penetrate the air/film interface, but insufficient to penetrate the film/Si interface Each minimum in this regime corresponds to an optical coupling condition, and the incident X- ray ... are not extremely sensitive to the X- ray beam passing through the origin; further refinements in the alignment of the X- ray optics are usually required For the θ -θ instrument depicted in Figure...

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Báo cáo lâm nghiệp: "Relationships between the intra-ring wood density assessed by X-ray densitometry and optical anatomical measurements in conifers. Consequences for the cell wall apparent density determination" pps

Ngày tải lên : 08/08/2014, 01:22

... density measured by x ray: apparent density of the cell wall = drx / cell wall proportion = drx · S/s = drx · R · T / [X · (T + R) – X2 ] = drx · R · T / [X · (T + R – X) ] 2.3.2 Hexagonal model ST ... varies, explaining a superior cell wall density in LW than in EW Yiannos [47] finds LW cell wall densities superior by at least 100 kg/m³ than in EW Wilfong’s [45] observations also point in that ... (left) and hexagonal (right) cell and lumen models Let X = · x = width of the double cell wall: s = R · T – [(R – X) · (T – X) ], s = X · (T + R) – X2 , s / S = cell wall proportion If drx = the wood...

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Báo cáo khao học: "Defining the transition from earlywood to latewood in black spruce based on intra-ring wood density profiles from X-ray densitometry" pot

Ngày tải lên : 08/08/2014, 14:20

... each ring from the pith to bark Ring density (RD) and ring width (RW) of each ring were determined based on the intra-ring microdensitometric profiles [11] Incomplete rings false rings and rings ... 60 80 100 Ring width (%) Figure Average within-ring density profile (from 100 trees) for the twentieth ring from pith showing the E/L transition point as determined by the inflexion point method ... previous findings for Norway spruce [1] Since the wood density at the E/L transition point defined by the inflexion point method 515 Earlywood-latewood transition in black spruce 0.7 90 Ring width...

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Báo cáo khoa học: "Use of kilovoltage X-ray volume imaging in patient dose calculation for head-and-neck and partial brain radiation therapy" pptx

Ngày tải lên : 09/08/2014, 08:23

... equipped with an integrated kV X- ray volumetric imaging system (Elekta, Synergy S, XVI, Crawley, UK) For imaging the head-and-neck and brain tumor patients, we used the following parameters: 100 ... sets Finally, the initial dose distribution matrix calculated on the planning CT was imported in the treatment planning system and displayed on the kV-CBCT dataset using scripts developed in- house ... air outside the patient skin in the planning system; however, the CBCT number in such situation is much greater than zero The mean CBCT value in the air around the skin is 1379 for the selected...

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$Báo cáo khoa học: "Intra-fraction setup variability: IR optical localization vs. X-ray imaging in a hypofractionated patient population" doc$

Báo cáo khoa học: "Intra-fraction setup variability: IR optical localization vs. X-ray imaging in a hypofractionated patient population" doc

Ngày tải lên : 09/08/2014, 09:20

... stereoscopic x- ray imaging of intrafraction patient motion in head and neck treatments using five points fixation masks Med Phys 2006, 33(2):504-13 Jin JY, Yin FF, Tenn SE, et al: Use of the BrainLABExacTrac ... thus taking into account the tumor excursion from exhale to inhale (Internal Margin) A slow CT scan was also acquired to ensure that tumor motion, during normal breathing, was included in the ... image-based procedures In 2006 Linhout et al [22] investigated the capabilities of the ExacTrac X- ray 6D system (BrainLab, Germany) in detecting intra-fraction motion in 13 head and neck patients...

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Báo cáo y học: "Diagnosis of periarticular osteoporosis in rheumatoid arthritis using digital X-ray radiogrammetry" ppsx

Ngày tải lên : 09/08/2014, 10:22

... metacarpal index (MCI), based on estimates of cortical thickness using digitized radiographs The computer algorithms employed in the DXR technique automatically define regions of interest around ... CAD technique exhibits excellent intra-radiograph (0.05% to 0.33%) and inter-radiograph (0.26% to 1.54%) reproducibility [5], providing confidence that estimated demineralization is in fact disease ... as indicated by DXR but not DXA, exhibit advanced hand demineralization; this was corroborated by further comparative studies [8-10] In comparison with other osteodensitometric techniques (DXA,...

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báo cáo khoa học: " Applying psychological theories to evidence-based clinical practice: identifying factors predictive of lumbar spine x-ray for low back pain in UK primary care practice" docx

Ngày tải lên : 10/08/2014, 10:23

... with low back pain for lumbar spine x- ray: ’When a patient presents with back pain, I have in mind to refer them for X- ray, I intend to refer patients with back pain for an X- ray as part of their ... lesser extent) behaviour Overall interpretation Low back pain is a frequent presenting problem in primary care settings However, the use of x- rays in clinical management of low back pain is relatively ... benefits; Indirect: In general, referring patients with back pain for an X- ray would reassure them (bb) x reassuring patients with back pain is (oe: un/important) Subjective Norm: Indirect (4...

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báo cáo khoa học: "Quantitative analysis of nanoparticle internalization in mammalian cells by high resolution X-ray microscopy" pps

Ngày tải lên : 11/08/2014, 00:23

... required staining Figure shows that even without staining this imaging method can readily explore in detail the dissimilarities in the internalization of different AuNPs by different cell lines Specifically, ... transmission x- ray microimages Transmission x- ray uptake microanalysis by EMT cells after co-culturing with 500 μM naked AuNPs for 48 h The size distribution in A) was obtained by analyzing individual ... kit (Vector Laboratories, Burlingame, CA) was used to perform DAB-nickel enhancement staining The metallic nickel-DAB mixture was imaged exploiting its strong x- ray absorption and used as a contrast...

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Báo cáo khoa hoc:" Body composition in male elite athletes, comparison of bioelectrical impedance spectroscopy with dual energy X-ray absorptiometry" pdf

Ngày tải lên : 11/08/2014, 07:20

... studies of athletes showing high reproducibility [3,13] DXA was originally developed to examine bone mineral density and examines the body in mm3 dividing the human body in three parts: bone, fatand ... -part II: utilization in clinical practice Clin Nutr 2004, 23:1430-1453 Lupoli L, Sergi G, Coin A, Perissinotto E, VOlpato S, Busetto L, Inelmen EM, Enxi G: Body composition in underweight elderly ... recruited during spring 2006 – spring 2007 from athletic clubs in the Göteborg region, Western Sweden All athletes were 18 years or older and competed in the highest Swedish league in their individual...

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Báo cáo y học: "Early changes in bone mineral density measured by digital X-ray radiogrammetry predict up to 20 years radiological outcome in rheumatoid arthritis" pps

Ngày tải lên : 12/08/2014, 15:22

... with being normal; being joint space narrowing, soft tissue swelling or periarticular osteoporosis; and to representing a progressively increasing degree of erosion and destruction A joint damage ... given in Figure There is a diverging trend during the whole follow-up period, which is most marked in the first years The increase in 95% CI at the later time points illustrates the increasing ... perceived pain, working situation, deteriorating function and so on The findings that early DXR-BMD loss in the cortical shaft of metacarpal bones is predictive of later progression of joint-related...

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