in situ enzyme activity—direct approach
ANALYSIS OF MICROBIAL COMMUNITY STRUCTURE AND IN SITU ACTIVITY OF NITRIFYING BIOFILMS
... distributions of in situ nitrifying activities were determined The relationship between the spatial organization of nitrifying bacterial populations and the in situ activity of these populations within the ... were performed In situ hybridization of vertical biofilm thin sections clearly indicated that the numbers of probe NEU-stained NH4+-oxidizing bacteria (i.e., Nitrosomonas marina-lineage, Nitrosomonas ... analyzed by comparing with existing 16S rRNA gene database and used for probe and primer designing The fingerprinting techniques can be used to monitor microbial community changes in natural environments...
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Báo cáo Y học: Restoring enzyme activity in nonfunctional low erucic acid Brassica napus fatty acid elongase 1 by a single amino acid substitution pdf
... cysteines present in other condensing enzymes To get more insight into how the coding region of the FAE1 condensing enzymes in Brassicas determines the proportions and amounts of VLCFAs in their ... threonine (e.g in Sorghum bicolor, broom corn) Indeed, the single-base change of nucleotide 845 from thymidine to cytosine, which resulted in the substitution of phenylalanine with serine in FAE1 ... examine the role of the amino acid serine at position 282 in the FAE1 protein sequence to determine if this apparent mutation from serine to phenylalanine led to the LEA B napus phenotype We introduced...
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Báo cáo khoa học: Identification of preferred substrate sequences for transglutaminase 1 – development of a novel peptide that can efficiently detect cross-linking enzyme activity in the skin pot
... The CE comprises several proteins, including involucrin, loricrin, trichohyalin and small proline rich-proteins, that are preferred substrates for TGases in the skin Of the eight TGase isozymes, ... 1, 2, and 5) are expressed in keratinocytes [13–18], and are involved in cross-linking of the structural proteins to form the CE at distinct intracellular sites [8,18] In the current model of TGase ... cadaverine (Dansyl-Cd) incorporated into recombinant peptide–GST(QN) fusion proteins (in which all the glutamine residues in glutathione S-transferase (GST) had been changed to asparagine) in the...
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Báo cáo khoa học: Identification of a preferred substrate peptide for transglutaminase 3 and detection of in situ activity in skin and hair follicles pdf
... cooperatively in the cross-linking of proteins, including involucrin, loricrin and small Preferred substrate peptide for TGase proline-rich proteins (SPRs) Such concerted reactions result in formation ... +1 Interestingly, the amino acid located at this position is not important for the reaction in other Tgases, including TGase In addition, at the N-terminal side of the glutamine residue including ... for detecting in situ activity of TGase in a frozen mouse skin section As shown in Fig 6, in the presence of CaCl2, specific incorporation of FITC–pepE51 (1 lm) in endogenous glutamine-acceptor...
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Báo cáo khoa học: The C-terminal region of the proprotein convertase 1⁄ 3 (PC1⁄ 3) exerts a bimodal regulation of the enzyme activity in vitro pdf
... N-terminal domains, thus rendering intriguing the possibility that PC1 ⁄ CT-peptide might by itself possess intrinsic inhibiting properties Production of recombinant CT-peptide We expressed in bacteria ... tethering, thus insuring cell-surface anchoring [37] In other cases, the CT-peptide contains integral transmembrane motifs affecting the sorting and recycling of furin and PC5 ⁄ 6B [38–40] In the case ... purification of recombinant mPC1 ⁄ and hfurin Recombinant murine PC1 ⁄ was produced using the baculovirus expression system in Sf9 insect cells [7] or through intracoelemic injection in insect larvae...
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Báo cáo khoa học: Human airway trypsin-like protease induces amphiregulin release through a mechanism involving protease-activated receptor-2-mediated ERK activation and TNF a-converting enzyme activity in airway epithelial cells doc
... collected by incubation in NaCl ⁄ Pi containing mm EDTA for at 37 °C After washing in NaCl ⁄ Pi containing 1% FBS, the cells were stained with anti-TACE mAb or control IgG at °C for 30 After washing with ... phosphorylated by its intrinsic receptor kinase through homo- and heterodimerization following ligand binding (autophosphorylation) and by nonreceptor tyrosine kinases such as Src family kinases [23,24], ... ERK activation through PAR-2 induces AR protein production but not protein release into the culture supernatant Since HAT-induced initial ERK activation results in induction of AR gene expression,...
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Báo cáo khóa học: Modulation of activity of NADH oxidase from Thermus thermophilus through change in flexibility in the enzyme active site induced by Hofmeister series anions ppt
... substrate-binding site leads to the increase in Km, i.e a decrease in the afnity of the enzyme for the substrate The decrease in kcat, however, can be only partially explained by the observed increase in ... avoprotein avodoxin from Desulfovibrio vulgaris, in the presence of phosphate anions, was interpreted as an increase in the dynamics of the tryptophan residue in the vicinity of the avin cofactor ... The thin line indicates dimeric interface The isoalloxasine ring of avin is localized in the rigid part of the homodimer, and Trp47 is localized on the most exible a-helix of the protein structure,...
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Báo cáo hóa học: " Double In Situ Approach for the Preparation of Polymer Nanocomposite with Multi-functionality" potx
... 1) We have called this a double in situ approach, because the in situ synthesis of the nanoparticle (TiO2) coincides with the in situ polymerization, resulting in the formation of a well-dispersed ... according to ASTM D2863-97 Vertical burning tests (UL-94) were conducted on a vertical burning test instrument (CZF-2-type) (Jiangning, China) with sheet dimensions of 130 13 mm3 according to ... spread but the presence of flaming drips, while V-0 shows self-extinguishing behaviour without burning drips While the increase in melt viscosity is to be expected on incorporation of well-dispersed...
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Báo cáo y học: " Hyperglycemia alters enzyme activity and cell number in spinal sensory ganglia" ppsx
... Optical density values fall along a range of representing minimal staining intensity (no staining) and 10 representing extremely dense staining (black) Page of (page number not for citation purposes) ... Nonetheless, the former interpretation is supported by the observed decrease in AP labeling in the dorsal horn of the spinal cord The observed decrease in AP intensity in the surviving small neuronal ... sensory neuron injury or repair (NADPH-diaphorase) The OVE26 transgenic mouse line (characterized by the insulin promoter-linked overexpression of calmodulin in pancreatic β-cells) used in this study...
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báo cáo khoa học: " Regulation of callose synthase activity in situ in alamethicin-permeabilized Arabidopsis and tobacco suspension cells" potx
... Aniline blue staining indicated that callose was deposited in spots over the cell surface, especially in walls connecting cells, but also in outer walls (walls facing the assay medium) Staining ... membrane-bound enzyme callose synthase in situ Digitonin was chosen for comparison when following UDPglucose incorporation, since this agent has been used in many investigations The activity measured (incorporation ... in Col-0 and BY-2 cells Each curve represents one independent experiment The maximum activity was 45 nmol min-1 g-1(FW) in Col-0 and 10 nmol min-1 g-1(FW) in BY-2 cells Omitting oryzalin during...
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Báo cáo y học: "Integration of metabolite with transcript and enzyme activity profiling during diurnal cycles in Arabidopsis rosettes" pptx
... (Coenzyme Q9) Respiration Glycine Serine Glycerate Photorespiration Glutamate Glutamine Aspartate Alanine Proline Homoserine Threonine Isoleucine Leucine Valine Methionine Shikimate Phenylalanine ... (Coenzyme Q9) Respiration Glycine Serine Glycerate Photorespiration Glutamate Glutamine Aspartate Alanine Proline Homoserine Threonine Isoleucine Leucine Valine Methionine Shikimate Phenylalanine ... (Coenzyme Q9) Respiration Glycine Serine Glycerate Photorespiration Glutamate Glutamine Aspartate Alanine Proline Homoserine Threonine Isoleucine Leucine Valine Methionine Shikimate Phenylalanine...
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development of a direct test method for dynamically assessing the liquefaction resistance of soils in situ
... obtained from integrating a single component of a 3D-MEMS accelerometer recorded during dynamic in- situ liquefaction testing; displayed in the: a) time domain, and b) frequency domain ... obtained from double integration of a single component of a 3D-MEMS accelerometer recorded during dynamic in- situ liquefaction testing; displayed in the: a) time domain, and b) frequency domain ... from a single component of a 3D-MEMS accelerometer during dynamic in- situ liquefaction testing; displayed in the: a) time domain, and b) frequency domain 110 Figure 6-3 Velocity signal obtained...
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A systematic approach for preferential crystallization thermodynamics, kinetics, optimal operation and in situ monitoring
... Characterization by the binary phase diagram 56 4.3.1.1 Melting point phase diagram of 4-hydroxy-2pyrrolidone 57 4.3.1.2 Melting point phase diagram of N-methylephedrine 65 4.3.1.3 Melting point phase diagram ... reported in literature The main objective of this project is to present a systematic approach to integrate thermodynamics, crystal nucleation and growth kinetics, optimal control and in- situ monitoring ... Figure 4.7 Determination of temperatures of fusion (Tf) for N-methylephedrine 65 Figure 4.8 Binary phase diagram (melting point diagram) of Nmethylephedrine 70 Figure 4.9 Determination of temperatures...
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Direct observations of nickel silicide formation on (100) si and si0 75ge0 25 substrates using in situ transmission electron microscopy
... and indexing of SAED pattern in Figure 5.1 (b) 121 A.3 Indexing of SAED pattern in Figure 5.2 (b) 124 A.4 Indexing of SAED pattern in Figure 5.4 (a) 125 A.5 Indexing of SAED pattern in ... for in- situ deposition of the nickel film using electron beam evaporation followed by the in- situ annealing using direct resistive heating Finally the data acquisition tools, the Gatan Imaging ... difficulties, mainly in obtaining low sheet resistance on narrow gates with conventional processing18,19,20 The increase in sheet resistance with decreasing line width can be attributed to incomplete...
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Tìm hiểu kỹ thuật lai huỳnh quang tại chỗ FISH (Florescence In Situ Hybridization) trên vi sinh vật
... for colonization of large intestines of streptomycin-treated mice Infect Immun 67, 6152–6156 [37] Felske, A., 1998 In situ detection of an uncultured predominant Bacillus in dutch grassland soils ... marginale DNA in bovine erythrocytes by slot-blot and in situ hybridization with a PCRmediated digoxigenin-labeled DNA probe J.Vet Diagn Invest 7, 465–472 [47] Gebhart, C.J., 1994 Specific in situ hybridization ... Boye, M., 1998 Specific detection of the genus Serpulina, S hyodysenteriae and S pilosicoli in porcine intestines by fluorescent rRNA in situ hybridization Mol Cell Probes 12, 323–330 [20] Brown-Howland,...
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Ứng dụng phương pháp in Situ hybridiza để chẩn đoán mầm bệnh WSSV trên tôm sú
... : In Situ hybridization PCR : Polymerase Chain Reaction ELISA : Enzyme Linked Immuno Sorbent Assay RT – PCR : Reverse – transcription polymerase chain reaction PBS : Phosphate Buffered Saline ... gắn biotin Sau trình lai, biotin diện 21 phân tử lai đƣợc phát cho ligand avidin (hay streptavidin) vào phản ứng Avidin (hay streptavidin) trƣớc có gắn với nhóm phát huỳnh quang hay enzyme xúc ... dụng là: Hệ thống sử dụng avidin (hay streptavidin) – biotin: cặp ligand có số lực cao tìm thấy sinh học Đầu tiên, mẫu dò đƣợc đánh dấu biotin theo hai cách: biotin đƣợc gắn vào DNA nhờ phản ứng...
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Tài liệu Báo cáo khoa học: Exposure of IgG to an acidic environment results in molecular modifications and in enhanced protective activity in sepsis doc
... same antigen– antibody interaction in the presence of increasing concentrations of NaCl In both experiments, binding intensities are represented in RU, and each data point shows the mean value ... bufferexposed IVIg to recombinant human IFN-c (D) Increased binding of low-pH buffer-exposed IVIg to human factor H In both panels, binding of the native IVIg is indicated by a solid line, and that of the ... mechanisms In addition to sepsis, there is an increasing number of emerging infectious diseases in which the severe generalized in ammatory reaction of the infected host is a major factor in the...
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