... local (i.e., paracrine) effects rather than circulating effects PDGF has mitogenic and chemotactic activity, on the target cells Many cells express receptors for PDGF, including some microvascular ... 1990) The appearance of the myofibroblasts corresponds to the commencement of connective tissue compaction and the contraction of the wound New collagen bundles in turn have the capacity to join ... enzymes or extracellularmatrix components which coordinate wound healing The cells and their secreted productsin turn affect the functioning of other cells through several signaling pathways,...
... of the MSP-1 gene) (5¢-TCC ATC ACC TCC ATT GCC TCC-3¢), corresponding to the amino acid sequences of the SGSSSSS and GGNGGDG of the MSP-1 gene, Total RNA was extracted with Isogen (Nippongene) ... on the organic matrixin molluscan shells-I Amino acid composition of the organic matrixinthe nacreous and prismatic layers Venus 47, 127–140 24 Samata T (1990) Ca-binding glycoproteins in ... 1827) (5¢-TCT GGC ATG AAA CAC GAC AAC-3¢), based on the nucleotide sequences of the 5¢ and 3¢ terminal regions, respectively TA cloning cDNA cloning Tissue collection for RNA extraction The outer...
... protein products were localized to theextracellular space determined that many of the protein products of these genes were involved in a variety of activities, including chemokine/cytokine activity ... MEK/ERK-dependent genes – whose products are found intheextracellular space – indicated that some of these genes were significantly categorized by the molecular function of their protein products into categories ... also induced by TNF in chondrocytes [36] In rat articular chondrocytes, macrophage Csf-1-induced signalling increases its own expression and the expression of the matricellular protein CCN2 (formerly...
... quantification of the transcriptomic interactions relating relevant biological themes and construction of functional interaction maps characterizing the transcriptomic signature of obese WAT inthe ... ECM components or involved in inflammatory processes In agreement, human pre-adipocytes cultured inthe presence of AcMCs increased their production of fibronectin and collagen type I, which formed ... Pancreatic cancer Chronic myeloid leukemia B cell receptor signaling pathway T cell receptor signaling pathway Glycosaminoglycan degradation Complement and coagulation cascades Glycan structures...
... 5'-GGA GCG CCG CCT GGA G-3' and reverse 5'-CCA TAT TCT TTC ACC GCC CAC TCC-3'; Invitrogen) Each PCR reaction contained μM of the respective forward and reverse primers, 1.5 mM MgCl2, 0.2 mM dNTPs ... since focal adhesion signaling, as defined inthe KEGG database, can be activated by cytokine-cytokine receptor interaction, which is also the major activation method of the JAK-STAT pathway In ... (mutagenic) changes, such as characteristic translocations [12] and the presence of epigenetic control [44] We should also now be able to monitor the effects of novel therapeutics on the cancer stem cell...
... by the World Health Organization Inthe case of chemical contaminants, the guidelines take the form of a maximum recommended concentration for a contaminant in drinking water Inthe case of acidity, ... However, the study found that the pollution incident did not cause an increased incidence of infection 1.30 There was no indication from the toxicological data on the contaminants of an adverse effect ... piping within the house In such properties the acidic water could have dissolved lead from the pipes, leading to increased concentrations inthe water inthe domestic system 3.18 Flushing of the...
... Syk in K562 cells (A) K562 cells stably expressing FcR c- chain (pRc /c) , or cotransfected with either wild-type GPVI and FcR c- chain (F1 /c) or a cytoplasmic-taildeleted GPVI and FcR c- chain (C1 /c) ... association with FcR c- chain Fig FcR c- chain and the cytoplasmic tail of GPVI are necessary to initiate the GPVI signalling cascade (A) K562 cells stably transfected with FcR c- chain and cotransfected ... erythroleukaemic cell line K562, which 2954 O Berlanga et al (Eur J Biochem 269) Fig Convulxin colocalizes at the membrane with the chimeric protein GPVI–GFP inthe absence of FcR c- chain COS-7 cells growing...
... Mut10 ACTAAGCTTAAAAGGGGACTTCTCTGGCATGGTTCAGGTTTGGAGGCACCTGGGA ACTAAGCTTAAAAGGGGACTTCTCTGGCATGGTTCCTGGGTGGAGGCACCTG ACTAAGCTTAAAAGGGGACTTCTCTGGCATGGGGCAGGGGTGGAGGCAC ACTAAGCTTAAAAGGGGACTTCTCTGGCAGTGTTCAGGGGTGGAGG ... ACTAAGCTTAAAAGGGGACTTCTCTGGCAGTGTTCAGGGGTGGAGG ACTAAGCTTAAAAGGGGACTTCTCTGTAATGGTTCAGGGGTGG ACTAAGCTTAAAAGGGGACTTCTAGGGCATGGTTCAGGGG ACTAAGCTTAAAAGGGGACTGATCTGGCATGGTTCAGGGG ACTAAGCTTAAAAGGGGCATTCTCTGGCATGGTTCAGGGG ACTAAGCTTAAAAGTTGACTTCTCTGGCATGGTTCAGGGG ... corepressor Complex by the receptor, which then recruits a series of coactivator proteins, such as steroid receptor coactivator (SRC-1), glucocorticoid receptor interacting protein (GRIP1), activator...
... to the thin black line; the calculated concentration control coecient is shown as a thick black line The dCTP pool size inthe reference strain CJ233 is shown by a stippled line decrease inthe ... promoter promoter integrated integrated integrated integrated integrated integrated integrated integrated integrated integrated integrated integrated inininininininininininin attB attB ... starved for CTP by resuspending cells in medium lacking cytidine thereby reducing the CTP pool size This results in accumulation of phosphatidic acid, the substrate for CDP-diacylglycerol synthetase...
... viruses, which are the main cause of various cancers, including cervical carcinoma In vitro data backed by recent in vivo studies suggest the existence of an elaborate sequence of cell surface events ... neither accessible in capsids nor in capsomeres [71] It remains unclear whether this antibody recognizes a speci c step in uncoating or reacts with protein inthe lysosomal compartment inthe ... intercapsomeric cleft to which the invading C- terminal arm contributes [15], suggesting that elements located within the cleft contribute to cell binding It is hoped that the determination of the...
... differentiation factor (GDF) (a 1299-bp fragment with sense primer: 5¢-GCAAGAGCAGGCA CCCAGCAACCAG-3¢ and antisense primer: 5¢-TTCCGT CACATAAAACCACAGCACT-3¢), follicle stimulating hormone (FSH) receptor ... 5¢-GAATCAAAGCCATACTGT-3¢), and vascular endothelial growth factor (VEGF) (a 612-bp fragment with sense primer: 5¢-TCAAGCCGTCCTGTG TGCCGCTGATGC-3¢ and antisense primer: 5¢-AGAAA ATGGCGAATCCAGTCCCACGAG-3¢) ... primer:CGTAAGGC TGTCTCTCATCAAAACT-3¢), bone morphogenetic protein (BMP) 15 (a 1057-bp fragment with sense primer: 5¢-CCCTGGCAAGGAGATGAAGCAATGG-3¢ and antisense primer: 5¢-GGGAAACCTGAGATAGCAACA ACTT-3¢),...
... one of theproducts of the reaction used to represent the cell cycle in each of the cells In this case, the attractor used to represent each cell was the Rossler attractor and each cell ran the ... Viewing continuous cultures of yeast as a stochastic tissue The details of the cellular dynamics that lead to the emergence of redox and TRAC oscillations and the gating of cells into cell-cycle ... formed the experimental foundation of efforts to synthesize a model of the cell cycle in which such disparate concepts as check points, and limit cycles or complex attractors were fused The basic...
... ATCAGGTCAGGTCAGCCATT TGCACGAGTCACACTGGAGC ATGCCGAATTCCTGGTCTGG GCAGAAGTCAACCAGACCGA GCAAGTATCCGCAGACGCTC AACGGCAAGATGCACATCAC ATATAGCACGGGATCATGGG GCTATGCTGGCTACCAGATG ATCAGCCTGCAGCACCAGAG ACACTCTACCACTGGCATCC ... ACACTCTACCACTGGCATCC GCTACTTGTTGTACTGCAGC CCTAGAACCGTGAAGAGCAT CAGGAAAGTCAGCTGCTATC ATGGCATCCAGTCCCTGTAT AAAGAACAGGAACTCTCCCC TCTGGTCTTCTGGCTCATGC GGTCAAGACCTAAGGAGTGG CATCACTGAGTTCCTGGACA CGATCAGCGTCATAGTCCTT ... FOR RC FOR RC FOR RC FOR RC FOR RC FOR RC FOR RC FOR RC FOR RC FOR RC FOR RC FOR RC FOR RC GTGACTTCAACAGTGACACC CCTTGGAGGCCATGTAGACC ATCGAAGGGGACTTCCGCTG ATCACCACACAGTCCTCTCCG GGCCTGTCTGCTTCTTGTAA...
... C3 22S C3 33S C3 43S C3 58S C3 67S C3 82S C3 93S C4 16S C4 22S C4 39S complete minimal reduced complete reduced minimal minimal minimal minimal minimal complete reduced minimal complete minimal Total protein ... an intervening cysteine-rich region A hydrophobic transmembrane domain is located near the C- terminus of the protein followed by a short (26 residues) cytoplasmic domain containing a single cysteine ... 32 C 33 C 34 C 35 C 36 C 38 C 39 C 41 C 42 C 43 W T L1 38 R Figure Fusion activity of cysteine mutations Fusion activity of cysteine mutations 293T cells were transfected with plasmids encoding...
... in contrast to findings for the LCMV glycoprotein where an endo H-resistant form of GP -C was described [22] However, since cleavage of Lassa virus GP -C by SKI-1/S1P occurs earlier inthe exocytotic ... streptomycin Antibodies Oligopeptide comprising the amino acids 477–491 of preGP -C was chemically synthesized and covalently linked to keyhole limpet hemocyanin (KLH, Pierce) as a carrier protein by the ... times with cold phosphate-buffered saline (PBS) and then cell surface was incubated twice for 15 at 4 C with mg/ml sulfo-Nhydroxysuccinimidobiotin (Pierce) by adding ml of the biotinylating reagent...
... 5'-CAACACTGCCAACGTCCAGAT-3' Reverse: 5'-CTGCTTCGTCCAGATAGGCAAT-3' Forward: 5'-ACTTCCGCTGGTCAGATGGA-3' Reverse: 5'-TCTCGTGCCAGATCATCACC-3' Forward 5'-CGGCCTGTTCCCCTTCTTCGTG-3' Reward 5'-TCGTGTGCTACGCTGCGGACCA-3' ... quantitative PCR Gene Primer sequences MMP-3 (NM_002422) Forward: 5'-CTTTTGGCGAAAATCTCTCAG-3' Reverse: 5'-AAAGAAACCCAAATGCTTCAA-3' Forward: 5'-AACTCCGACATCGTGATCCG-3' Reverse: 5'-GTAGTAGCAGGACTTG ATCT-3' ... chondrocyte and synovial fibroblasts, may cause an imbalance inextracellularmatrix (ECM) turnover, accelerate the degradation of the cartilage matrix, and also increase the incidence of chondrocyte...
... product, packaging material is sure, secure, certified check safety products To the logo and colors of the product: Logo needs to be printed clearly and set in front of packaging, the product with ... Product Commercialization Commercialization of the product is actually launched the product inthe market It’s not an independent work It involves strategy, resources of business, policy, infrastructure ... lifecycle Accordingly, in order to have development, testing and launching new products and services On this discussion, the remaining new product and service development stages is made clearly...
... it can be deduced that the SnO2 nanocrystals are indeed introduced inthe silica xerogel From the UV–Vis spectrum of the silica xerogel containing Sm3+ ions and SnO2 nanocrystals (Fig 3), it can ... here), suggesting that the size of SnO2 nanocrystals increases These results further confirm that the SnO2 nanocrystals are incorporated inthe silica matrix, and the network of silica and SnO2 ... the emission of silica gels No characteristic emission of Sm3+ ions can be observed for the silica xerogel containing Sm3+ ions (curve a), while the sample containing SnO2 nanocrystals and Sm3+...