in breast cancer cells mechanisms functional correlates and potential for a new therapeutic target
Báo cáo khoa học: BRCA1 accumulates in the nucleus in response to hypoxia and TRAIL and enhances TRAIL-induced apoptosis in breast cancer cells pdf
Ngày tải lên :
30/03/2014, 03:20
... evidence linking hypoxia, TRAIL signaling and BRCA1 in mammary epithelial cells Our data demonstrate that hypoxia induces nuclear accumulation of BRCA1 without altering its protein level, and that this ... that TRAIL can alter BRCA1 localization and induce apoptosis in breast cancer cells Therefore, we assessed the requirement for functional BRCA1 in TRAIL-stimulated apoptosis HCC1937 cells have ... DR4 and DR5, and recruits Fas-associated death domain protein to the receptors Caspase-8 is activated and subsequently activates downstream caspases Hypoxia inhibits TRAIL-mediated apoptosis in...
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ANTI CANCER EFFECTS OF THYMOQUINONE IN BREAST CANCER CELLS INVOLVEMENT OF NON HOMOLOGOUS END JOINING AND TELOMERE TELOMERASE HOMEOSTASIS
Ngày tải lên :
30/09/2015, 13:41
... than normal cells on the remaining functional DNA repair mechanisms for damage repair (Damia and D' Incalci, 2007) A B Figure Possible sources of DNA damage, DNA repair mechanisms and subsequent ... novel therapeutic agents for human malignancies CHAPTER 2 Materials and Methods 2.1 Cell lines and drug treatment Two human breast carcinoma cell lines, MCF-7 and MDA-MB-231 (ATCC, Manassas, VA, ... with Streptavidin-Cy3 (GE Healthcare, Bio-Sciences, UK) Illumina Bead Array Reader was used to scan the arrays The array data thus obtained after scanning was imported and analysed using Partek®...
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EFFECTS OF INHIBITING THE MAMMALIAN TARGET OF RAPAMYCIN (MTOR) PATHWAY AND TELOMERASE IN BREAST CANCER CELLS
Ngày tải lên :
05/10/2015, 13:53
... advantage gained by mTOR activation in cancer cells and the increasing knowledge on the implications of mTOR in promoting and maintaining malignant transformation have placed mTOR as a critical anticancer ... rapamycin may function as a dual inhibitor of mTOR and telomerase, sustained rapamycin treatment leading to Akt activation may play a role in resistance via telomerase activation in some breast ... anticancer therapeutic target Ongoing clinical trials are testing the efficacy of rapamycin and its analogues, cell cycle inhibitor (CCI)-779 and RAD001 against nearly all major forms of cancers...
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Investigating functions of ERp29 in mesenchymal to epithelial transition (MET) and epithelial plasticity in breast cancer cells
Ngày tải lên :
13/10/2015, 15:55
... CGAGAGACTCCGGTTCCTA-3’ Forward 5’-AGCGAACTGGACACACATAC-3’ Reverse 5’-TCTAGACTGGGCATCGCAG-3' Forward 5’-GTCCCTGGAGGAGAAAGACC-3’ Reverse 5’-CTGCTTTGGGATTCAGGTTC-3’ Forward 5’-GCAAGCTTAGAGATGATGCAGGACG-3’ ... Mesenchymal to epithelial transition Myosin light chain Phosphorylated myosin light chain Matrix metalloproteinase Mammalian target of rapamycin Nuclear factor kappa beta p21-activated kinase-1 Protein ... Reverse 5’-GACTCGAGGTGGGACGCGGACATGGA-3’ Forward 5'-CTGCTGGGAGGATGACAGA-3' Reverse 5'-ATCCTGCTTCATCTGCCTGA-3' Forward 5'-AGTCCATGCGAACTGCCATCTGAT-3' Reverse 5'- CTGGACCATCTACAGAGGCTTGTA-3' Forward 5’-...
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Báo cáo Y học: Progestin upregulates G-protein-coupled receptor 30 in breast cancer cells pot
Ngày tải lên :
24/03/2014, 00:21
... the total RNA Statistical analysis Data for the correlation analysis were handled using a regression analysis of SSPI and/ or a correlation analysis of EXCEL Data for the growth studies and immunochemistry ... (Amersham Pharmacia Biotech) were used for PCR: GPR30-forward, 5¢-AGTCGG ATGTGAGGTTCAG-3¢; GPR30-reverse, 5¢-TCTGTGT GAGGAGTGCAAG-3¢; TBP-forward, 5¢-TTTGGAAG AGCAACAAAGG-3¢; TBP-reverse, 5¢-AAGGGTGCAG ... proliferation of MCF-7 cells may suggest a common target of the growth factors and steroid hormones Progestin administration causes an initial increase in cell proliferation lasting about 12–24 h and...
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Báo cáo khoa học: "Postmastectomy irradiation in breast in breast cancer patients with T1-2 and 1-3 positive axillary lymph nodes: Is there a role for radiation therapy" ppsx
Ngày tải lên :
09/08/2014, 09:20
... nodes in the infra or supraclavicular fossa or in the internal mammary chain was considered as a regional recurrence Any recurrence outside these areas was defined as DM Statistical Analysis LRR and ... DMe Alive DM Death DM Death DM Death DM Death DM Alive Out-come a Lateral, bMedial, cInvasive ductal carcinoma, dInvasive Paget’s disease, eDistance metastasis patients had lung metastasis and ... followed LRR Clinical characteristics of patients with LRR are shown in Table LRRs’ histologic types were invasive ductal carcinoma for patients and invasive lobular carcinoma Cosar et al Radiation Oncology...
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Heregulin enhanced tyrosine phosphorylation in breast cancer cells role of PP2A in HER 2 eu oncogenic signalling
Ngày tải lên :
11/09/2015, 10:06
... extracellular ligand-binding domain with two cysteine-rich regions, a hydrophobic short transmembrane domain, and an intracellular domain that contains a catalytic tyrosine kinase domain and a carboxyl ... extracellular ligand binding ectodomain, a hydrophobic membrane-spanning domain (3 kDa) and a short juxtamembrane segment, and an intracellular tyrosine kinase domain (70-90 kDa) linked to a carboxyl ... BRCA1 and BRCA2, which are responsible for familial breast and ovarian cancers Although these mutations cause approximately 5-10% of all breast cancer cases, they are rare in the general population...
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Báo cáo y học: "elf-harm in British South Asian women: psychosocial correlates and strategies for prevention" pot
Ngày tải lên :
08/08/2014, 21:20
... patterns and trends in people of Indian subcontinent and Caribbean origin in England and Wales Ethnicity and Health 1996, 1(1):55-63 Raleigh VS, Balarajan R: Suicide and self-burning among Indians ... mentioned in terms of Pakistani, Bangladeshi, and Indian etc In most studies concerning self-harm and Asians, Pakistani, Indian and Bangladeshi subjects were all generally placed under the same category ... the two largest ethnic minorities in England and Wales i.e Indian and West Indian Results show that Indian males have low Standardised Mortality Rates (73) as compared to the Indian females (115)...
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REBUILDING AMERICA’S DEFENSES Strategy, Forces and Resources For a New Century doc
Ngày tải lên :
07/03/2014, 10:20
... revolution in military affairs to offset American advantages in naval and air power, for example If the United States is to retain the technological and tactical advantages it now enjoys in large-scale ... upon maintaining and expanding a world-wide system of alliances as well as on the ability to win wars While maintaining its combat role, the U.S Army has acquired new missions in the past decade ... provided a United States blueprint for maintaining U.S faces no preeminence, global rival precluding the rise America’s of a great power rival, and shaping grand strategy the international should aim...
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báo cáo khoa học: " Salivary a-amylase exhibits antiproliferative effects in primary cell cultures of rat mammary epithelial cells and human breast cancer cells" docx
Ngày tải lên :
10/08/2014, 10:21
... cell carcinoma of the breast J Clin Pathol 2005, 55:545-547 Tanahashi C, Yasuki S, Akamine N, Yatabe Y, Ichihara S: Pure acinic cell carcinoma of the breast in an 80-year-old Japanese woman Pathol ... blind- and non-blind-performed investigations a- Amylase treatment in human mammary epithelial cells The effect of a- amylase in mammary cells of human origin was studied in primary HBCEC (mammary ... predominant aamylase in the mammary gland [12] Heitlinger et al [13] suggested that a- amylase type in the breast milk compensates for low salivary and pancreatic activity in newborns by improving...
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European guidelines for quality assurance in breast cancer screening and diagnosis pot
Ngày tải lên :
15/03/2014, 00:20
... lessons learned in the European Breast Cancer Network in which scientists, clinicians and paramedical staff as well as advocates, health care planners and administrators across Europe have shared ... QUALITY ASSURANCE IN BREAST CANCER SCREENING 1.1 Introduction That a breast cancer screening programme can reduce breast cancer mortality in the age group 40-74 years has been shown in several randomised ... responsibility for mammographic image quality and diagnostic interpretation They must understand the risks and benefits of breast cancer screening and the dangers of inadequately trained staff and sub-optimal...
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Báo cáo Y học: Rapid caspase-dependent cell death in cultured human breast cancer cells induced by the polyamine analogue N1,N11-diethylnorspermine pdf
Ngày tải lên :
17/03/2014, 23:20
... were incubated at 37 °C in a water-saturated atmosphere containing 5% CO2 in air The growth of the cells was monitored at each passage by counting in a haemocytometer and the cells were regularly ... Potent DNAdamaging agents are commonly used in cancer chemotherapy and tumour regression after chemotherapy is caused, at least in part, by the ability of DNA damaging agents to activate apoptosis ... analogues are presently undergoing Phase I and Phase II clinical evaluations in the US In L56Br-C1 cells, DENSPM treatment induced an increase in SSAT activity, which resulted in a decrease in...
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Detection of breast cancer cells using targeted magnetic nanoparticles and ultra-sensitive magnetic field sensors potx
Ngày tải lên :
22/03/2014, 17:20
... analysis and interpretation HCB participated in analysis and interpretation of nanoparticle characterization data RSL participated in experiment design and data interpretation ERF participated in experiment ... in an incubator at 37°C with 5% CO2 and maintained at a cell concentration of between × 105 and × 10 viable cells/ mL MDA-MB-231 cells were cultured in an incubator at 37°C with no CO2 and maintained ... collection and interpretation DF participated in experiment execution (TEM measurements) and data collection and interpretation TCM participated in nanoparticle characterization and data collection...
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Báo cáo khoa học: Vitamin D stimulates apoptosis in gastric cancer cells in synergy with trichostatin A ⁄sodium butyrate-induced and 5-aza-2¢-deoxycytidine-induced PTEN upregulation ppt
Ngày tải lên :
22/03/2014, 21:20
... are shown as means ± standard deviations RNA isolation and quantitative real-time PCR Total RNA was extracted following the TaKaRa RNAiso Reagent manual, and reverse transcribed into cDNA using ... further investigations into the potential application of vitamin D as a novel molecular target in gastric cancer therapies in association with the use of TSA ⁄ NaBu and 5-Aza Results Vitamin D induced ... DNA methylation and subsequently upregulated PTEN expression in acute lymphoblastic leukemia, melanoma and ovarian cancer cells [27–29] In gastric cancer cells, treatment with 5-Aza can greatly...
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Báo cáo khoa học: Ixocarpalactone A isolated from the Mexican tomatillo shows potent antiproliferative and apoptotic activity in colon cancer cells pot
Ngày tải lên :
23/03/2014, 10:20
... untreated and treated SW480 cells All bands were compared with b-actin bands using densitometric analysis The percentage change for each protein compared with b-actin bands is indicated as up-regulation ... Mehta RG (2001) Cruciferous vegetables and cancer prevention Nutr Cancer 41, 17–28 13 Aggarwal BB, Kumar A & Bharti AC (2003) Anticancer potential of curcumin: preclinical and clinical studies Anticancer ... To investigate the effects of IxoA on apoptosis, we 5720 used acridine orange ⁄ ethidium bromide staining and DAPI staining and found a marked increase in the percentage of apoptotic cells in...
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Targeting New Pathways and Cell Death in Breast Cancer Edited by Rebecca L. Aft docx
Ngày tải lên :
23/03/2014, 17:20
... Wang, Raza Ali, Bassam Bitar, Farah T Logna, Main Y Maitah, Bin Bao, Shadan Ali, Dejuan Kong, Yiwei Li and Fazlul H Sarkar Chapter Multidrug Resistence and Breast Cancer Gengyin Zhou and Xiaofang ... to Clinical Relevance Philipp Y Maximov and V Craig Jordan Chapter Targeted Apoptosis in Breast Cancer Immunotherapy 23 Lin-Tao Jia and An-Gang Yang Chapter Induction of Apoptosis in Human Cancer ... overexpression in breast cancers correlates with high metastasis capacity, increased disease recurrence and worse prognosis, and are therefore routinely examined in breast cancer patients for a determination...
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báo cáo hóa học:" ST6Gal-I expression in ovarian cancer cells promotes an invasive phenotype by altering integrin glycosylation and function" pptx
Ngày tải lên :
20/06/2014, 07:20
... regarding the functional consequences of ST6Gal-I upregulation in ovarian carcinoma Casey and colleagues treated OVCAR5 ovarian carcinoma cells with neuraminidase enzyme to remove sialic acids and ... information has been available regarding the role of this enzyme in ovarian cancer The accumulating evidence indicating that ST6Gal-I-mediated integrin sialylation causes increased cell migration ... Absorbance (540 nm) Cells expressing ST6Gal-I show increased haptotactic migration on collagen I A hallmark of advanced ovarian carcinoma is intraperitoneal spread, and therefore cancer cells...
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báo cáo hóa học:" Curcumin induces chemo/radio-sensitization in ovarian cancer cells and curcumin nanoparticles inhibit ovarian cancer cell growth" pot
Ngày tải lên :
20/06/2014, 07:20
... Kunnumakkara AB, Yadav VR, Tekmal RR, Aggarwal BB: Design of curcumin-loaded PLGA nanoparticles formulation with enhanced cellular uptake, and increased bioactivity in vitro and superior bioavailability ... treatment in cisplatin resistant cells by increasing the sensitivity of cells to apoptotic pathways and modulating nuclear β-catenin signaling Curcumin is in early phase clinical trials for various ... Chauhan SC, Vannatta K, Ebeling MC, Vinayek N, Watanabe A, Pandey KK, Bell MC, Koch MD, Aburatani H, Lio Y, Jaggi M: Expression and functions of transmembrane mucin MUC13 in ovarian cancer Cancer...
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báo cáo hóa học:" MUC4 stabilizes HER2 expression and maintains the cancer stem cell population in ovarian cancer cells" ppt
Ngày tải lên :
20/06/2014, 07:20
... Farooq A, Carothers Carraway CA, Salas PJ, Carraway KL: Muc4-ErbB2 complex formation and signaling in polarized CACO-2 epithelial cells indicate that Muc4 acts as an unorthodox ligand for ErbB2 ... Conception and design, financial support, data analysis and interpretation, manuscript writing, and final approval of manuscript Competing interests The authors declare that they have no competing interests ... data, data analysis and interpretation, manuscript writing PS.: Experimental help and data analysis AV: Data analysis and manuscript writing PD.: Experimental help and manuscript writing SKB:...
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