... psychrotrophic RNase HI ina localizedfashion as revealed by mutational and X-raycrystallographic analysesMuhammad S. Rohman1, Takashi Tadokoro1, Clement Angkawidjaja1, Yumi Abe1,Hiroyoshi Matsumura2,3, ... extension: tailor-made genes using thepolymerase chain reaction. Biotechniques 8, 528–535.34 Kanaya S, Katsuda C, Kimura S, Nakai T, Kitakuni E,Nakamura H, Katayanagi K, Morikawa K & IkeharaM ... thermal denaturation at Tmwere calcu-lated by van’t Hoff analysis.Urea-induced denaturationUrea-induced denaturation curves of So-RNase HI and itsderivatives were measured at 20 °C as described...
... trauma, infection, intrauterine catastrophes 16 or head surgery17. In our case, the lack ofa previous history of trauma, infection and head surgery leads us to believe that the AC was ... oculopharyngeal muscular dystrophy. Clin Neurosci. 2003; 10: 125-7. 22. Orlacchio A, Gaudiello F, Totaro A, et al. A new SPG4 mutation in a variant form of spastic paraplegia with congenital arach-noid ... Neuroimaging of the twins. (a) Cerebral CT of twin A shows a vast lesion of cerebrospinal fluid intensity in the left temporal lobe with a maximum diameter of 63×40×26 mm. (b) & (c) MRI of twin...
... For, above all, it was assumed, a colonist must bemature.And in that assumption, Duran concluded, had been hidden the fal-lacy which had made a fiasco of the project. For was not maturity largely a ... theNuclear Fission Society of Urania, Nevada. It is not a well publicized factthat this quasi-academic group of adolescent physicists was exposed in the act of assembling an elementary but workable ... had said that everything was all right, but with an uneasiness in the way he spoke. Grades, maybe, Duran thought. The boy had been do-ing pretty well, almost as well as Roger, but was showing...
... tourists and organizations. In the words of Barbara Czarniawska, today’s organizations are facedwith thefrantic production of images which are circulated; a frantic translation of incoming images into ... reducingour use of material and nonrenewable energy and a myriad of tools and concepts embracing presented as tools for achieving sustainability (including initiatives such as LCA, dematerialization, ... under-standing of sustainable development in general and sustainable consumption in particular.Contemporary Studies of Sustainable Consumption A vast number of articles focusing on sustainable...
... EXECUTIVE COACHING and sometimes unknowable situations, and our training, conceptual models, and professional skill are often insufficient in the face ofa chaotic organizational landscape. I have ... in any reasonably sized organization. As a result, this complex- ity forces a narrowing of focus to those elements that are considered crucial to organizational or, in the case ofa coaching ... sponsoring corporation. They were engaged fully and working hard as individuals and asa class. The cost of the workshop was approximately $40,000 plus hotel, travel, and meals for each participant....
... envelhecimento, memória, cognição, testes neuropsicológicos.Behavioral and Cognitive Neurology Unit, Department of Internal Medicine, Faculty of Medicine, Federal University of Minas Gerais, Belo Horizonte, Brazil.Paulo Caramelli – Department of Internal Medicine / Faculty of Medicine / Federal University of Minas Gerais - Avenida Prof. Alfredo Balena, 190 / Room 246 - 30130-100 Belo Horizonte MG - Brazil. E-mail: caramelp@usp.brReceived 01/30/2008. Received in final form 02/19/2008. Accepted 02/19/2008.44 ... 2008;2(1):42-45Memory loss is one of the most common complaints arising in consultations with elderly people, being reported by 25% to 50% of these individuals.1 However, whether these subjective memory complaints (SMC) are related to objective memory deficits or to subsequent development of dementia, remains a matter of debate. A recent review found that SMCs are not consistently associated with current cognitive impairment, but rather are associated with a greater risk of future cognitive de-cline.2 Indeed, the diagnosis of mild cognitive impairment (MCI), which entails an increased likelihood of conversion to dementia, demands the existence of SMCs, preferably confirmed by an informant.3High age, female gender and low educational level are generally associated with a higher prevalence of memory complaints.1 In an autopsy study, SMCs were found to be related to the presence of Alzheimer’s disease (AD) pathol-ogy in elderly with and without dementia, suggesting that memory complaints in older persons may be a sign of self awareness ofa degenerative process.4However, SMCs might also be related to depression and some personality traits, such as neuroticism.2 It is also pos-sible that these complaints vary according to the culture of the people studied. Ina recent Brazilian study, Minett et al. found that subjects with and without SMCs performed similarly ina series of cognitive tests, although the former had higher scores on the Geriatric Depression Scale.5The present study aimed to further investigate this topic in a group of cognitively healthy Brazilian elderly subjects which were divided into two subgroups according to the presence of SMCs and submitted to brief cognitive tests.MethodsSixty cognitively intact elderly individuals (39 females and 21 males), aged 69.9±6.3 years (ranging from 60 to 91 years), and with mean educational level of 8.5±5.5 years (ranging from 1 to 20 years), were included in the study. These individuals were family caregivers of demented pa-tients followed at the Behavioral and Cognitive Neurology Unit of the Faculty of Medicine of the Federal University of Minas Gerais, in Belo Horizonte (MG), Brazil, and also volunteers recruited from the community.Inclusion criteria were absence of neurological or psy-chiatric diseases according to a clinical interview, absence of depression (see below), and no use of benzodiazepines, antidepressants or neuroleptics. All participants were submitted to the Mini-Mental State-Examination (MMSE)6,7 and to the Cornell scale of depression.8,9 Performance on the MMSE was adjusted for educational level and had to be greater than or equal to 21 for 1-3 years of schooling, greater than or equal to 24 for 4-7 years and greater than or equal to 26 for individuals with 8 or more years of schooling.10 Scores on the Cornell scale of depression had to be less than or equal to 7 points in order to rule out depression.8Cognitive evaluation was carried out with the follow-ing tests: the Brief Cognitive Screening Battery (BCSB)11,12, digit span forward and backward and the Frontal Assess-ment Battery (FAB).13,14 The BCSB includes a memory test of 10 simple figures and yields different scores, namely: incidental and immediate memory, learning, delayed recall and recognition.15,16 The battery also includes a category fluency test (animals per minute) and clock drawing and has proven very sensitive in the diagnosis of mild AD.12 The FAB is a brief diagnostic instrument for the assessment of executive functions in patients with suspected frontal lobe syndrome.13All individuals were given a structured self-report memory questionnaire, the MAC-Q.17 This questionnaire was devised to assess age-related memory decline. It is composed by six questions related to memory function-ing in everyday situations (e.g., to remember a telephone number that he/she uses at least once a week) in which the subject is asked to compare and rate his/her current ability to when he/she was 40 years’ old. The total score on the MAC-Q ranges from 7 to 35, where greater scores in- dicate subjective memory loss. Scores greater than or equal to 25 have been found to be suggestive of age-associated memory impairment. Accordingly, in the present study, the individuals were divided into two groups: absence of SMCs (MAC-Q scores <25) and presence of SMCs (MAC-Q scores ≥25). The performance of the two groups on the different cognitive tests was compared.One of the authors administered the MMSE, the Cor-nell scale and the MAC-Q. Subsequently, the other inves-tigator, blinded to the subjects’ results for these three mea-sures, administered the cognitive evaluation.Descriptive analysis of the data and statistical compari-sons between the performances of the two groups on the different cognitive tests were carried out with MedCalc software. Student’s t-test was used for comparison of age, educational level and MMSE scores, as well as for the results of the other cognitive tests (digit span, BCSB and FAB). Chi-square was employed for comparing gender distribu-tion of the two groups. Level of significance was set at 0.05. The study was approved by the Research Ethics ... 2008;2(1):42-45Memory loss is one of the most common complaints arising in consultations with elderly people, being reported by 25% to 50% of these individuals.1 However, whether these subjective memory complaints (SMC) are related to objective memory deficits or to subsequent development of dementia, remains a matter of debate. A recent review found that SMCs are not consistently associated with current cognitive impairment, but rather are associated with a greater risk of future cognitive de-cline.2 Indeed, the diagnosis of mild cognitive impairment (MCI), which entails an increased likelihood of conversion to dementia, demands the existence of SMCs, preferably confirmed by an informant.3High age, female gender and low educational level are generally associated with a higher prevalence of memory complaints.1 In an autopsy study, SMCs were found to be related to the presence of Alzheimer’s disease (AD) pathol-ogy in elderly with and without dementia, suggesting that memory complaints in older persons may be a sign of self awareness ofa degenerative process.4However, SMCs might also be related to depression and some personality traits, such as neuroticism.2 It is also pos-sible that these complaints vary according to the culture of the people studied. Ina recent Brazilian study, Minett et al. found that subjects with and without SMCs performed similarly ina series of cognitive tests, although the former had higher scores on the Geriatric Depression Scale.5The present study aimed to further investigate this topic in a group of cognitively healthy Brazilian elderly subjects which were divided into two subgroups according to the presence of SMCs and submitted to brief cognitive tests.MethodsSixty cognitively intact elderly individuals (39 females and 21 males), aged 69.9±6.3 years (ranging from 60 to 91 years), and with mean educational level of 8.5±5.5 years (ranging from 1 to 20 years), were included in the study. These individuals were family caregivers of demented pa-tients followed at the Behavioral and Cognitive Neurology Unit of the Faculty of Medicine of the Federal University of Minas Gerais, in Belo Horizonte (MG), Brazil, and also volunteers recruited from the community.Inclusion criteria were absence of neurological or psy-chiatric diseases according to a clinical interview, absence of depression (see below), and no use of benzodiazepines, antidepressants or neuroleptics. All participants were submitted to the Mini-Mental State-Examination (MMSE)6,7 and to the Cornell scale of depression.8,9 Performance on the MMSE was adjusted for educational level and had to be greater than or equal to 21 for 1-3 years of schooling, greater than or equal to 24 for 4-7 years and greater than or equal to 26 for individuals with 8 or more years of schooling.10 Scores on the Cornell scale of depression had to be less than or equal to 7 points in order to rule out depression.8Cognitive evaluation was carried out with the follow-ing tests: the Brief Cognitive Screening Battery (BCSB)11,12, digit span forward and backward and the Frontal Assess-ment Battery (FAB).13,14 The BCSB includes a memory test of 10 simple figures and yields different scores, namely: incidental and immediate memory, learning, delayed recall and recognition.15,16 The battery also includes a category fluency test (animals per minute) and clock drawing and has proven very sensitive in the diagnosis of mild AD.12 The FAB is a brief diagnostic instrument for the assessment of executive functions in patients with suspected frontal lobe syndrome.13All individuals were given a structured self-report memory questionnaire, the MAC-Q.17 This questionnaire was devised to assess age-related memory decline. It is composed by six questions related to memory function-ing in everyday situations (e.g., to remember a telephone number that he/she uses at least once a week) in which the subject is asked to compare and rate his/her current ability to when he/she was 40 years’ old. The total score on the MAC-Q ranges from 7 to 35, where greater scores in- dicate subjective memory loss. Scores greater than or equal to 25 have been found to be suggestive of age-associated memory impairment. Accordingly, in the present study, the individuals were divided into two groups: absence of SMCs (MAC-Q scores <25) and presence of SMCs (MAC-Q scores ≥25). The performance of the two groups on the different cognitive tests was compared.One of the authors administered the MMSE, the Cor-nell scale and the MAC-Q. Subsequently, the other inves-tigator, blinded to the subjects’ results for these three mea-sures, administered the cognitive evaluation.Descriptive analysis of the data and statistical compari-sons between the performances of the two groups on the different cognitive tests were carried out with MedCalc software. Student’s t-test was used for comparison of age, educational level and MMSE scores, as well as for the results of the other cognitive tests (digit span, BCSB and FAB). Chi-square was employed for comparing gender distribu-tion of the two groups. Level of significance was set at 0.05. The study was approved by the Research Ethics...
... theReports and found that he was right in every particular, and a page and a half of that judgment is really in that way the work of General Smuts ratherthan myself.15Foxton continues that Shaw was ... politicians of the twentieth century. As well asa stint as Prime Minister of South Africa, he was a member of the Imperial War Cabinet duringthe First World War, played a significant role in the ... looksmainly to cases decided in the United Kingdom, Australia, and Canada in order to show that law provides a moral resource that can inform a rule -of- law project capable of responding to situations...
... But my argument is in part based in the fact that the kinds of claims that are made about states of emergency occur also in quite ordinary situations, for example, claimsthat the rule of law does ... greatly weakened. Indeed, LordWoolf’s reasoning in Belmarsh sustains Simpson’s charge of judicial lipservice to the rule of law as, in an obvious, face-saving ploy, Lord Woolfwarned against ... constitution of Commonwealth countries. It looksmainly to cases decided in the United Kingdom, Australia, and Canada in order to show that law provides a moral resource that can inform a rule -of- law project...
... agudeza visual, grado de dificultad para caminar 300 metros, incapacidad pararealizar las actividades cotidianas debido a problemas de salud en las 2 semanas pre-vias y seropositividad a Trypanosoma ... instru-ments, training of field-work and labo-ratory teams, and adjusting for severalconfounding variables. Internal valid-ity was assured because the elderlyparticipants in the baseline of the Bam-buí ... esa relación se mantienedurante la edad avanzada. El objetivo de este trabajo fue examinar la asociación entrela situación socioeconómica y la salud en adultos mayores de una peque a localidadde...
... answering the phone at state and local of ces. Include accurate information on what happened and what is being done. Pro-vide information about how the state and local associations are involved. ... meeting as soon as practical to talk with staff and express support and caring. Practice active listening, allowing staff to safely vent and be heard. Make decision about classes the next day at ... active listening and acknowledge pain and anxiety as well asa healing process that has ups and downs. Return to normalcy. It’s important for everyone to return to a routine as quickly as possible...
... returns that are lower than the lending rate offered bythe bank. Providing that a bank cannot undo completely the decrease in its profits from increased borrowingrates by increasing its lending rates, ... on each firm withcertainty. A coordination game results if banks are assumed to play a Nash strategy in theirchoice of industry. The outcome of this game determines the inter-bank correlation.If ... inter-bank linkages in contagion.169.3 Herding Linked to Manager ial ReputationScharfstein and Stein (1990), Rajan (1994) modeled herding behavior based on managerialconcerns for reputation.17Scharfstein...
... effort has already been invested in mathematicalmodelling of the glycolytic pathway in yeast [3–8] and in other organisms, such as Trypanosoma brucei, the parasitethat causes sleeping sickness ... metaboliteconcentration and flux are obtained from populations of yeast cells and so reflect an aggregate of the states of many individual organisms. Although no fitted model in thispaper individually ... role for hexokinase and the remainder of main-chain glycolysis having only minimal relevance. This patternhas previously been observed ina study of glycolytic fluxcontrol in rat heart perfused...